Labshake search
Citations for Jena Bioscience :
1 - 50 of 164 citations for Cyclic GMP cGMP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... To assemble microtubules stabilized with guanylyl-(a,(3)-methylene-diphosphonate (GMP-CPP) (Jena Bioscience), tubulin was diluted to 2 mg/mL in BRB80 buffer supplemented with 0.5 mM GMP-CPP ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 mM GTP (Roth, K056.4) or 1 mM GMP (Jena Biosciences, JBS-NU-1028), was added to the washed beads and incubated gently by rocking for 30 min at 4°C ...
-
bioRxiv - Biophysics 2020Quote: ... Tubulin solutions were mixed with 1 µl 10 mM GMP-CPP (#NU-405S, Jena Bioscience) on ice and incubated for 10 minutes to allow nucleotide exchange ...
-
bioRxiv - Plant Biology 2023Quote: ... for degradation of bound GDP to GMP and Pi in the presence of 1.5-fold molar excess of non-hydrolyzable GTP analog GppCp (Jena Bioscience, Germany). After confirmation of GDP degradation by HPLC ...
-
bioRxiv - Plant Biology 2023Quote: ... then 5×106 cells were plated on ESAW agar plates containing 450 μg mL-1 nourseothricin (Jena Bioscience), and incubated in constant light at 18 °C.
-
bioRxiv - Microbiology 2021Quote: ... The assay was performed as per kit instructions using a Tecan Spark 10M multimode plate reader and ATP (nM) was determined using the ATP (Jena Bioscience) standard curve produced.
-
bioRxiv - Genetics 2021Quote: ... the PCR labelling Cy-3 and Cy-5 kits (Jena Bioscience, ref# PP-301L-Cy3 and #APP-101-Cy5) according to the manufacturer’s instructions with M13 universal primers ...
-
bioRxiv - Biophysics 2021Quote: ... initial crystallization conditions were screened on sitting-drop Greiner 609120 96-well plates using a Honeybee963 robot (Digilab) and commercial kits from Jena Bioscience (Jena) and Hampton Research (Aliso Viejo ...
-
bioRxiv - Biophysics 2020Quote: ... of λ-DNA using the following primers: 5’-CGAACTCTTCAAATTCTTCTTCCA-3’ and 5’-GATTGCTCTTCTGTAAGGTTTTG-3’ with a 5:1 ratio of dTTP:biotin-11-dUTP (Jena Bioscience). Similarly ...
-
bioRxiv - Biochemistry 2022Quote: Initial crystallization conditions for P9-1ΔC-arm were screened at room temperature on 96-well sitting-drop Greiner 609120 plates using a Digilab Honeybee963 robot (Marlborough, USA) and commercial kits from Jena Bioscience (Jena, Germany) and Hampton Research (Aliso Viejo ...
-
bioRxiv - Molecular Biology 2023Quote: ... Additional 5-Ethynyl-uridine (5-EU) was purchased from Jena Bioscience (CLK-N002) and resuspended to an initial stock concentration of 400 mM in DMSO.
-
bioRxiv - Molecular Biology 2021Quote: ... The 100% m6A modified full-length PAN transcript was synthesized using the MEGAscript T7 Transcription Kit following the manufacturer’s protocol but substituting adenosine-triphosphate with N6-methyladenosine-5’-triphosphate (Jena Bioscience NU-1101S). RNAs were purified using the MEGAclear Transcription Clean-Up Kit (ThermoFisher AM1908).
-
bioRxiv - Molecular Biology 2019Quote: ... 5 % CO2 for 1 h after a final concentration of 500 µM 5-Ethynyluridine (5EU, Jena Bioscience) was added ...
-
bioRxiv - Microbiology 2019Quote: ... 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne ...
-
bioRxiv - Cell Biology 2022Quote: ... 5 mM AF647-Picolyl-azide (Jena Biosciences) and 10 mM sodium ascorbate (Sigma ...
-
bioRxiv - Biochemistry 2022Quote: ... 2’-deoxythymidine-5’-[α-thio]-triphosphate (dTTPαS) and 2’-deoxyguanosine-5’-[α-thio]-triphosphate (dGTPαS) were obtained from Jena Biosciences. N-methylanthraniloyl guanosine-5’-triphosphate (mant-GTP ...
-
bioRxiv - Cell Biology 2019Quote: ... nascent RNA was labelled by incubating the cells with 400 μM 5-ethynyluridine (5-EU; Jena Bioscience; CLK-N002-10,), which was then visualized with a click-iT mix consisting of 50mM Tris buffer pH8 ...
-
bioRxiv - Molecular Biology 2022Quote: ... in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680, NU-821-680, Jena bioscience) in each reaction ...
-
bioRxiv - Plant Biology 2019Quote: ... 5-Ethynyl-UTP was purchased from Jena Bioscience. A fraction of the reaction was analyzed using a standard agarose gel ...
-
Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience) at 2 mM and MgCl2 at 4 mM was incubated for 15 minutes at room temperature ...
-
bioRxiv - Biochemistry 2020Quote: ... 5’-guanylyl imidodiphosphate (GDPNP; Jena Bioscience NU-401-50) was added to the wells 16 minutes after the start of the reaction for five minutes at a concentration of 100uM followed by a five-minute pretreatment of either 208uM emetine (Cayman Chemical 21048 ...
-
bioRxiv - Cell Biology 2022Quote: ... and 5 mM ATP (Jena Biosciences; NU-1010-SOL)) supplemented with 220 nM Sytox Orange at 100 μl/min ...
-
bioRxiv - Molecular Biology 2023Quote: ... 250 μM ppGpp (Guanosine-3’,5’-bisdiphosphate) (Jena Bioscience).
-
bioRxiv - Immunology 2023Quote: ... 10 μM EdU (5-ethynyl-2’-deoxyuridine, Jena Bioscience) was added to the cell culture medium for 10 min at the end of 72 h culture on anti-CD3ε- and anti-CD28-coated cell culture plates ...
-
bioRxiv - Microbiology 2023Quote: ... 5 µl biotin-11-CTP (1 mM) (Jena Bioscience), 0.8 µl DTT (0.5 M ...
-
bioRxiv - Cell Biology 2023Quote: ... 5-fold molar excess of DBCO-AF647 (Jena Bioscience) was added to the azide-coupled Nb followed by incubation for 2 hours at room temperature and subsequently purified by dialysis and hydrophobic interaction chromatography (HIC).
-
bioRxiv - Genomics 2023Quote: ... N1-Methylpseudouridine-5’-Triphosphate (m1meΨTP, Jena Bioscience, NU-890S) instead of UTP or N4-Acetyl-cytidine-5’-triphosphate (N4-Acetyl-CTP ...
-
bioRxiv - Zoology 2020Quote: ... in which a third of the dTTP had been replaced with Digoxigenin-X-(5-aminoallyl)-2’-deoxyuridine-5’-triphosphate (Jena Bioscience, Jena, Germany) for 40 cycles ...
-
Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyguanosine-5’-triphosphate (3’-dGTP, Jena Bioscience) and 4 mM MgCl2 ...
-
Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience), 4 mM MgCl2 and 2 μM G-actin ...
-
bioRxiv - Neuroscience 2023Quote: ... 0,2 µM 5/6 TAMRA-PEG4-Alkyne tag (Jena Biosciences) and 0,2 mM freshly prepared CuSO4 ...
-
bioRxiv - Biochemistry 2022Quote: ... 5 mM of m7GP3G monomethylated cap analogue (Jena Bioscience, NU-852) was soaked into mature NSP14 crystals (grown in 1.26 M sodium phosphate monobasic ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with 5’ethynyluridine (EU) (0.5 mM) (Jena Bioscience) and Click-iT Alexa Fluor 647 labelling kit (Thermofisher ...
-
bioRxiv - Molecular Biology 2024Quote: Cells seeded in 6-well plates were treated with 20 μM OP-puro (Jena Bioscience) at 37°C for 30 min ...
-
bioRxiv - Genomics 2022Quote: ... with 50% supplement of 5-Methyl-CTP and Pseudo-UTP (Jena Biosciences), following the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2021Quote: ... and N1-methyladenoside 5’-phosphate (N1-methyl-AMP) was from Jena Bioscience. MTase-Glo™ Methyltransferase Assay Kit containing SAM and SAH were purchased from Promega ...
-
bioRxiv - Cancer Biology 2023Quote: EDA-m7GTP-5-FAM (NU-824-5FM) was purchased from Jena Bioscience and used as received ...
-
bioRxiv - Immunology 2023Quote: ... Cells were pulsed with OPP (20μM, Jena Bioscience, cat: NU-931-5) in complete RPMI supplemented with the cytoplasmic translation inhibitor harringtonine (1μM ...
-
bioRxiv - Microbiology 2022Quote: ... Transformants were selected onto YPD agar plates containing 200 μg/mL nourseothricin (Jena Bioscience GmbH, Germany) and screened by colony PCR to confirm the presence of the mutation (Table S2) ...
-
bioRxiv - Biophysics 2019Quote: ... Cy3 labeled 2’/3’-O-(2-aminoethyl-carbamoyl)-adenosine-5’-triphosphate (Jena Bioscience) was added into the imaging buffer instead of ATP.
-
bioRxiv - Biochemistry 2021Quote: ... For labeling pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl)phosphate (Jena bioscience), was ligated to the 3’ end of the 16 nt RNA using T4 RNA ligase (Thermo Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 30 min incubation with 50μM OPP (Jena Bioscience, NU-913-5). Cultures were then washed with room temperature PBS and fixed with 4 % of PFA ...
-
bioRxiv - Cell Biology 2023Quote: ... This media was changed and ATP (5 mM) (Jena Biosciences cat. NU-1010) was added for 24 h prior to collection of conditioned media ...
-
bioRxiv - Cell Biology 2023Quote: ... This media was changed and ATP (5 mM) (Jena Biosciences cat. NU-1010) was added for 24 h prior to collection of conditioned media ...
-
bioRxiv - Biochemistry 2023Quote: ... 5 mM of each triphosphate ribonucleotide (standard nucleotides were purchased from Jena Bioscience GmBH and pseudouridine and N1-methylpseudouridine from BOC sciences) ...
-
bioRxiv - Neuroscience 2020Quote: ... AMP-CPP (α,β-methyleneadenosine 5’-triphosphate lithium salt, Jena Bioscience #NU-421-25), AMP-PCP (β,γ-Methyleneadenosine 5′-triphosphate disodium salt ...
-
bioRxiv - Neuroscience 2022Quote: ... 0.4 μM 5/6- TAMRA-PEG 4 -Alkyne (Jena Bioscience; Jena, Germany; CLK-TA108) and 0.2 mM freshly prepared CuSO4 in 1x PBS pH7.8 ...
-
bioRxiv - Plant Biology 2023Quote: ... DNA oligonucleotides used as probes were extended with 5-Azido-PEG4-dCTP (Jena Bioscience) and terminal transferase (NEB) ...
-
bioRxiv - Molecular Biology 2019Quote: ... 100 % of UTP (resp. GTP) was substituted with either 5-ethynyl-UTP (5EU, Jena Bioscience), 5-bromo-UTP (5BrU ...
-
bioRxiv - Molecular Biology 2022Quote: ... 100 pmols of RNA were incubated with 150 pmoles of pCp-Cy-5 (Jena Biosciences), 20 units of T4-RNA ligase (NEB) ...