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Citations for Jena Bioscience :
1 - 50 of 165 citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... To assemble microtubules stabilized with guanylyl-(a,(3)-methylene-diphosphonate (GMP-CPP) (Jena Bioscience), tubulin was diluted to 2 mg/mL in BRB80 buffer supplemented with 0.5 mM GMP-CPP ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM GTP (Roth, K056.4) or 1 mM GMP (Jena Biosciences, JBS-NU-1028), was added to the washed beads and incubated gently by rocking for 30 min at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... Tubulin solutions were mixed with 1 µl 10 mM GMP-CPP (#NU-405S, Jena Bioscience) on ice and incubated for 10 minutes to allow nucleotide exchange ...
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bioRxiv - Plant Biology 2023Quote: ... for degradation of bound GDP to GMP and Pi in the presence of 1.5-fold molar excess of non-hydrolyzable GTP analog GppCp (Jena Bioscience, Germany). After confirmation of GDP degradation by HPLC ...
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bioRxiv - Genetics 2023Quote: ... The probes were obtained by direct labeling of the bacterial DNA (1.5 μg) using the Nick Translation kit from Jena Bioscience (Atto488 NT Labeling Kit), the labeling reaction was performed at 15°C for 90 min ...
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bioRxiv - Genomics 2023Quote: ... mycoides probe was obtained by direct labeling of the bacterial DNA (1.5 µg) using the Nick Translation kit from Jena Bioscience (Atto488 NT Labeling Kit). For the 2µ plasmid labeling ...
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bioRxiv - Plant Biology 2023Quote: ... then 5×106 cells were plated on ESAW agar plates containing 450 μg mL-1 nourseothricin (Jena Bioscience), and incubated in constant light at 18 °C.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alkyne labelled proteins were enriched and affinity purified by direct attachment to azide agarose beads (Jena Bioscience) using copper catalysed click chemistry ...
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bioRxiv - Microbiology 2021Quote: ... The assay was performed as per kit instructions using a Tecan Spark 10M multimode plate reader and ATP (nM) was determined using the ATP (Jena Bioscience) standard curve produced.
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bioRxiv - Genetics 2021Quote: ... the PCR labelling Cy-3 and Cy-5 kits (Jena Bioscience, ref# PP-301L-Cy3 and #APP-101-Cy5) according to the manufacturer’s instructions with M13 universal primers ...
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bioRxiv - Biophysics 2021Quote: ... initial crystallization conditions were screened on sitting-drop Greiner 609120 96-well plates using a Honeybee963 robot (Digilab) and commercial kits from Jena Bioscience (Jena) and Hampton Research (Aliso Viejo ...
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bioRxiv - Biophysics 2020Quote: ... of λ-DNA using the following primers: 5’-CGAACTCTTCAAATTCTTCTTCCA-3’ and 5’-GATTGCTCTTCTGTAAGGTTTTG-3’ with a 5:1 ratio of dTTP:biotin-11-dUTP (Jena Bioscience). Similarly ...
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bioRxiv - Biochemistry 2022Quote: Initial crystallization conditions for P9-1ΔC-arm were screened at room temperature on 96-well sitting-drop Greiner 609120 plates using a Digilab Honeybee963 robot (Marlborough, USA) and commercial kits from Jena Bioscience (Jena, Germany) and Hampton Research (Aliso Viejo ...
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bioRxiv - Molecular Biology 2023Quote: ... Additional 5-Ethynyl-uridine (5-EU) was purchased from Jena Bioscience (CLK-N002) and resuspended to an initial stock concentration of 400 mM in DMSO.
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bioRxiv - Molecular Biology 2021Quote: ... The 100% m6A modified full-length PAN transcript was synthesized using the MEGAscript T7 Transcription Kit following the manufacturer’s protocol but substituting adenosine-triphosphate with N6-methyladenosine-5’-triphosphate (Jena Bioscience NU-1101S). RNAs were purified using the MEGAclear Transcription Clean-Up Kit (ThermoFisher AM1908).
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bioRxiv - Molecular Biology 2019Quote: ... 5 % CO2 for 1 h after a final concentration of 500 µM 5-Ethynyluridine (5EU, Jena Bioscience) was added ...
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bioRxiv - Microbiology 2019Quote: ... 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM AF647-Picolyl-azide (Jena Biosciences) and 10 mM sodium ascorbate (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... 2’-deoxythymidine-5’-[α-thio]-triphosphate (dTTPαS) and 2’-deoxyguanosine-5’-[α-thio]-triphosphate (dGTPαS) were obtained from Jena Biosciences. N-methylanthraniloyl guanosine-5’-triphosphate (mant-GTP ...
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bioRxiv - Cell Biology 2019Quote: ... nascent RNA was labelled by incubating the cells with 400 μM 5-ethynyluridine (5-EU; Jena Bioscience; CLK-N002-10,), which was then visualized with a click-iT mix consisting of 50mM Tris buffer pH8 ...
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bioRxiv - Molecular Biology 2022Quote: ... in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680, NU-821-680, Jena bioscience) in each reaction ...
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bioRxiv - Plant Biology 2019Quote: ... 5-Ethynyl-UTP was purchased from Jena Bioscience. A fraction of the reaction was analyzed using a standard agarose gel ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience) at 2 mM and MgCl2 at 4 mM was incubated for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 5’-guanylyl imidodiphosphate (GDPNP; Jena Bioscience NU-401-50) was added to the wells 16 minutes after the start of the reaction for five minutes at a concentration of 100uM followed by a five-minute pretreatment of either 208uM emetine (Cayman Chemical 21048 ...
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bioRxiv - Cell Biology 2022Quote: ... and 5 mM ATP (Jena Biosciences; NU-1010-SOL)) supplemented with 220 nM Sytox Orange at 100 μl/min ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 μM ppGpp (Guanosine-3’,5’-bisdiphosphate) (Jena Bioscience).
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bioRxiv - Immunology 2023Quote: ... 10 μM EdU (5-ethynyl-2’-deoxyuridine, Jena Bioscience) was added to the cell culture medium for 10 min at the end of 72 h culture on anti-CD3ε- and anti-CD28-coated cell culture plates ...
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bioRxiv - Microbiology 2023Quote: ... 5 µl biotin-11-CTP (1 mM) (Jena Bioscience), 0.8 µl DTT (0.5 M ...
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bioRxiv - Cell Biology 2023Quote: ... 5-fold molar excess of DBCO-AF647 (Jena Bioscience) was added to the azide-coupled Nb followed by incubation for 2 hours at room temperature and subsequently purified by dialysis and hydrophobic interaction chromatography (HIC).
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bioRxiv - Genomics 2023Quote: ... N1-Methylpseudouridine-5’-Triphosphate (m1meΨTP, Jena Bioscience, NU-890S) instead of UTP or N4-Acetyl-cytidine-5’-triphosphate (N4-Acetyl-CTP ...
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bioRxiv - Zoology 2020Quote: ... in which a third of the dTTP had been replaced with Digoxigenin-X-(5-aminoallyl)-2’-deoxyuridine-5’-triphosphate (Jena Bioscience, Jena, Germany) for 40 cycles ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyguanosine-5’-triphosphate (3’-dGTP, Jena Bioscience) and 4 mM MgCl2 ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience), 4 mM MgCl2 and 2 μM G-actin ...
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bioRxiv - Neuroscience 2023Quote: ... 0,2 µM 5/6 TAMRA-PEG4-Alkyne tag (Jena Biosciences) and 0,2 mM freshly prepared CuSO4 ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM of m7GP3G monomethylated cap analogue (Jena Bioscience, NU-852) was soaked into mature NSP14 crystals (grown in 1.26 M sodium phosphate monobasic ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with 5’ethynyluridine (EU) (0.5 mM) (Jena Bioscience) and Click-iT Alexa Fluor 647 labelling kit (Thermofisher ...
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bioRxiv - Molecular Biology 2024Quote: Cells seeded in 6-well plates were treated with 20 μM OP-puro (Jena Bioscience) at 37°C for 30 min ...
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bioRxiv - Genomics 2022Quote: ... with 50% supplement of 5-Methyl-CTP and Pseudo-UTP (Jena Biosciences), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... and N1-methyladenoside 5’-phosphate (N1-methyl-AMP) was from Jena Bioscience. MTase-Glo™ Methyltransferase Assay Kit containing SAM and SAH were purchased from Promega ...
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bioRxiv - Cancer Biology 2023Quote: EDA-m7GTP-5-FAM (NU-824-5FM) was purchased from Jena Bioscience and used as received ...
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bioRxiv - Immunology 2023Quote: ... Cells were pulsed with OPP (20μM, Jena Bioscience, cat: NU-931-5) in complete RPMI supplemented with the cytoplasmic translation inhibitor harringtonine (1μM ...
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bioRxiv - Microbiology 2022Quote: ... Transformants were selected onto YPD agar plates containing 200 μg/mL nourseothricin (Jena Bioscience GmbH, Germany) and screened by colony PCR to confirm the presence of the mutation (Table S2) ...
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bioRxiv - Biophysics 2019Quote: ... Cy3 labeled 2’/3’-O-(2-aminoethyl-carbamoyl)-adenosine-5’-triphosphate (Jena Bioscience) was added into the imaging buffer instead of ATP.
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bioRxiv - Biochemistry 2021Quote: ... For labeling pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl)phosphate (Jena bioscience), was ligated to the 3’ end of the 16 nt RNA using T4 RNA ligase (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 30 min incubation with 50μM OPP (Jena Bioscience, NU-913-5). Cultures were then washed with room temperature PBS and fixed with 4 % of PFA ...
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bioRxiv - Cell Biology 2023Quote: ... This media was changed and ATP (5 mM) (Jena Biosciences cat. NU-1010) was added for 24 h prior to collection of conditioned media ...
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bioRxiv - Cell Biology 2023Quote: ... This media was changed and ATP (5 mM) (Jena Biosciences cat. NU-1010) was added for 24 h prior to collection of conditioned media ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM of each triphosphate ribonucleotide (standard nucleotides were purchased from Jena Bioscience GmBH and pseudouridine and N1-methylpseudouridine from BOC sciences) ...
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bioRxiv - Neuroscience 2020Quote: ... AMP-CPP (α,β-methyleneadenosine 5’-triphosphate lithium salt, Jena Bioscience #NU-421-25), AMP-PCP (β,γ-Methyleneadenosine 5′-triphosphate disodium salt ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 μM 5/6- TAMRA-PEG 4 -Alkyne (Jena Bioscience; Jena, Germany; CLK-TA108) and 0.2 mM freshly prepared CuSO4 in 1x PBS pH7.8 ...