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Citations for Jena Bioscience :
1 - 50 of 182 citations for 3' 3' Cyclic GAMP cGAMP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience) at 2 mM and MgCl2 at 4 mM was incubated for 15 minutes at room temperature ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyguanosine-5’-triphosphate (3’-dGTP, Jena Bioscience) and 4 mM MgCl2 ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience), 4 mM MgCl2 and 2 μM G-actin ...
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bioRxiv - Biophysics 2020Quote: ... of λ-DNA using the following primers: 5’-CGAACTCTTCAAATTCTTCTTCCA-3’ and 5’-GATTGCTCTTCTGTAAGGTTTTG-3’ with a 5:1 ratio of dTTP:biotin-11-dUTP (Jena Bioscience). Similarly ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 μM ppGpp (Guanosine-3’,5’-bisdiphosphate) (Jena Bioscience).
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were labeled at the 3’ end using pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl) phosphate) (Jena Biosciences). For each binding experiment ...
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bioRxiv - Biophysics 2020Quote: ... 5’-TAGTCCAGAACGAGACCGCAACAGCACAACCCAAACTG-3’ and 5’-AATCTGCTGCAATGCCACAG-3’ (underline section indicates overhang) with a 10:1 ratio of dTTP:digoxigenin-11-dUTP (Jena Bioscience). The labelled fragments were purified using a PCR purification kit (QIAquick ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3’deoxy GTP (3′dGTP) from Jena Bioscience.
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bioRxiv - Genetics 2021Quote: ... the PCR labelling Cy-3 and Cy-5 kits (Jena Bioscience, ref# PP-301L-Cy3 and #APP-101-Cy5) according to the manufacturer’s instructions with M13 universal primers ...
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bioRxiv - Biophysics 2019Quote: ... Cy3 labeled 2’/3’-O-(2-aminoethyl-carbamoyl)-adenosine-5’-triphosphate (Jena Bioscience) was added into the imaging buffer instead of ATP.
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bioRxiv - Biochemistry 2021Quote: ... For labeling pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl)phosphate (Jena bioscience), was ligated to the 3’ end of the 16 nt RNA using T4 RNA ligase (Thermo Scientific) ...
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Tail Length and E525K Dilated Cardiomyopathy Mutant Alter Human β-Cardiac Myosin Super-Relaxed StatebioRxiv - Molecular Biology 2023Quote: The fluorescence of 3’-O-(N-Methyl-anthraniloyl)-2’-deoxyadenosine-5’-triphosphate (mantATP) (Jena Biosciences) was measured with 290 nm excitation and a 395 nm long-pass emission filter in a stopped-flow apparatus (Applied Photophysics ...
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bioRxiv - Molecular Biology 2022Quote: ... in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680, NU-821-680, Jena bioscience) in each reaction ...
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bioRxiv - Biochemistry 2020Quote: ... 3’dGTP and cytidine-5’-[(α,β)-methyleno]triphosphate (CMPCPP) were from Jena Bioscience (Jena, Germany); 2’dGTP ...
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bioRxiv - Molecular Biology 2022Quote: ... ≥95% purity for 3’-dGTP (Jena Bioscience)] and incubated 2 min at 37°C (to “chase,” and thereby to stabilize ...
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bioRxiv - Molecular Biology 2022Quote: ... ≥95% purity for 3’-dGTP (Jena Bioscience)] and incubated 2 min at 37°C (to “chase” non-terminated complexes) ...
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bioRxiv - Cell Biology 2023Quote: ... 3 µM Picolyl-Alexa647-Azide (Jena Bioscience) and 80 µM ascorbic acid (Merck) ...
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bioRxiv - Biophysics 2020Quote: ... and 3 mM GMPCPP (NU-405S, Jena Bioscience) was mixed on ice ...
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bioRxiv - Biochemistry 2021Quote: ... RNAs were fluorescently labeled with cyanine 5 at the 3’ end with the help of pCp-Cy5 (Jena Bioscience, 84179) and T4 RNA ligase I (NEB ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... and 3-azido-7-hydroxycoumarin (Jena Bioscience, CLK-FA047). LD540 dye was a kind gift from Christoph Thiele (University of Bonn ...
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bioRxiv - Biophysics 2019Quote: ... 2’-(3’)-bis-O-(N-methylanthraniloyl)-GDP (Jena Biosciences). Rab5 was loaded with mant-GDP in Rab5 buffer + 10mM EDTA and 5-fold excess mant-GDP ...
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bioRxiv - Biochemistry 2019Quote: ... 3’ end-labelled using Aminoallyl-UTP-Cy5 (Jena Bioscience) incubated with TdT.
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bioRxiv - Genetics 2019Quote: ... EdU was coupled to a cleavable biotin-azide linker (Azide-PEG(3+3)-S-S-biotin) (Jena Biosciences, Cat. No. CLK-A2112-10) using the reagents of the Click-it Kit (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: Guanine nucleotide exchange activity was measured by following changes in the fluorescence of the GDP derivative mant-dGDP [2’-Deoxy-3’-O-(N-Methyl-anthraniloyl) guanosine-5’-diphosphate sodium salt (Jena Bioscience GmbH)] (Margarit et al. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... In-vitro transcription was conducted with HighYield T7 Cap 1 AG (3‘-OMe) mRNA Synthesis Kit (m5CTP) (Jena Bioscience, Germany) using 800 ng of amplified template followed by Turbo DNase treatment (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: 3’-(O-Propargyl)-ATP (NU-945) was offered by Jena Biosciences. To complete the set ...
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bioRxiv - Molecular Biology 2023Quote: T7 promoter containing double stranded DNA (Supplementary Table 3) was used as a template for in vitro transcription with HighYield T7 mRNA Synthesis Kit (ac4CTP) (Jena Bioscience).
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bioRxiv - Cell Biology 2023Quote: The in vitro transcribed RNAs were 3’ biotinylated using pCp-Biotin (Jena Biosciences). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... The pre-tRNAs were 3’ biotinylated with pCp-biotin (750 µM; Jena Bioscience) and T4 RNA ligase (2 units/µl) ...
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bioRxiv - Biochemistry 2022Quote: ... To assemble microtubules stabilized with guanylyl-(a,(3)-methylene-diphosphonate (GMP-CPP) (Jena Bioscience), tubulin was diluted to 2 mg/mL in BRB80 buffer supplemented with 0.5 mM GMP-CPP ...
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bioRxiv - Genomics 2020Quote: ... Female genomic probe was labelled with Cy3-dUTP (cyanine 3-deoxyuridine triphosphate; Jena Bioscience, Germany) by nick translation ...
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bioRxiv - Microbiology 2022Quote: ... and 1 μL of 3-azido-7-hydroxycoumarin solution (1 mM in EtOH; Jena Bioscience) was added ...
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bioRxiv - Biophysics 2024Quote: ... a 3:1 BrdU/BrdC mix (Sigma-Aldrich, #B5002, and Jena Bioscience, #N-DN-6496) was added to the cells ...
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bioRxiv - Immunology 2021Quote: ... XCL1-K(N3) and sdAb-K(N3) were subsequently reacted with DBCO-Cy5.5 (3 equiv.) (CLK-1046, Jena BioSciences) overnight at RT on an end-over-end shaker ...
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bioRxiv - Biochemistry 2020Quote: Guanosine nucleotides labeled with fluorescent N-methyl-anthranoyl (mant) groups O-linked with 2’ or 3’ of ribose (Jena Biosciences), were used for binding studies ...
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bioRxiv - Plant Biology 2023Quote: ... then 5×106 cells were plated on ESAW agar plates containing 450 μg mL-1 nourseothricin (Jena Bioscience), and incubated in constant light at 18 °C.
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Dried lipid films were click reacted in a 40 μl reaction mix containing 0.45 mM fluorogenic dye 3-azido-7-hydroxycoumarin (Jena Bioscience, CLK-FA047), 1.4 mM Cu(I)tetra(acetonitrile ...
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bioRxiv - Evolutionary Biology 2024Quote: ... strains with MYO3 or MYO5 locus fused at its 3’ to the DHFR F[1,2] with a nourseothricin-resistance marker (NAT 100 μg/ml, Jena Bioscience GmbH, Germany). To insert the variant libraries instead of the wild-type SH3 domains ...
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bioRxiv - Biophysics 2022Quote: ... The 2’-deoxy-ATP labeled with N-Methylanthraniloyl at the 3’-ribose position (mantATP) was also purchased from Jena Biosciences (10 mM stock). ATP and ADP were prepared from powder (MilliporeSigma ...
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bioRxiv - Genomics 2023Quote: ... 3 µL of 1 mM each dATP/ddCTP/ddGTP/ddTTP (dATP/ddBTP) (dATP, Thermo Fisher R0141; ddCTP/ddGTP/ddTTP, Jena Bioscience NU-1019S), and 7.5 U of Klenow fragment 3’→5’ exo-(NEB) ...
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bioRxiv - Microbiology 2021Quote: ... The assay was performed as per kit instructions using a Tecan Spark 10M multimode plate reader and ATP (nM) was determined using the ATP (Jena Bioscience) standard curve produced.
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bioRxiv - Biophysics 2021Quote: ... initial crystallization conditions were screened on sitting-drop Greiner 609120 96-well plates using a Honeybee963 robot (Digilab) and commercial kits from Jena Bioscience (Jena) and Hampton Research (Aliso Viejo ...
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bioRxiv - Biochemistry 2022Quote: Initial crystallization conditions for P9-1ΔC-arm were screened at room temperature on 96-well sitting-drop Greiner 609120 plates using a Digilab Honeybee963 robot (Marlborough, USA) and commercial kits from Jena Bioscience (Jena, Germany) and Hampton Research (Aliso Viejo ...
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bioRxiv - Molecular Biology 2023Quote: ... Additional 5-Ethynyl-uridine (5-EU) was purchased from Jena Bioscience (CLK-N002) and resuspended to an initial stock concentration of 400 mM in DMSO.
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bioRxiv - Molecular Biology 2021Quote: ... The 100% m6A modified full-length PAN transcript was synthesized using the MEGAscript T7 Transcription Kit following the manufacturer’s protocol but substituting adenosine-triphosphate with N6-methyladenosine-5’-triphosphate (Jena Bioscience NU-1101S). RNAs were purified using the MEGAclear Transcription Clean-Up Kit (ThermoFisher AM1908).
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bioRxiv - Molecular Biology 2019Quote: ... 5 % CO2 for 1 h after a final concentration of 500 µM 5-Ethynyluridine (5EU, Jena Bioscience) was added ...
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bioRxiv - Microbiology 2019Quote: ... 5-Tetramethylrhodamine-Alkyne (TAMRA-Alkyne; Jena Bioscience), Acetylene-PEG4-Biotin (Biotin-Alkyne ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM AF647-Picolyl-azide (Jena Biosciences) and 10 mM sodium ascorbate (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... 2’-deoxythymidine-5’-[α-thio]-triphosphate (dTTPαS) and 2’-deoxyguanosine-5’-[α-thio]-triphosphate (dGTPαS) were obtained from Jena Biosciences. N-methylanthraniloyl guanosine-5’-triphosphate (mant-GTP ...
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bioRxiv - Cell Biology 2019Quote: ... nascent RNA was labelled by incubating the cells with 400 μM 5-ethynyluridine (5-EU; Jena Bioscience; CLK-N002-10,), which was then visualized with a click-iT mix consisting of 50mM Tris buffer pH8 ...