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Citations for Cytoskeleton :
1 - 48 of 48 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-CDC42 (Cytoskeleton Inc., ACD03, 1:250 for Western blot); mouse monoclonal anti-Rac1 (Merck Millipore ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal protein levels were confirmed by a control western blot of rabbit anti-Kinesin (Cytoskeleton) from the cleared lysates ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed by western blot using anti-ubiquitin antibody (P4D1, Cytoskeleton AUB01-HRP, 1:1000) and visualized with Clarity Western ECL Substrate (BioRad).
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bioRxiv - Cell Biology 2023Quote: ... The samples were stored at −20°C prior to western blot analysis for actin (Cytoskeleton 1:1000). Standards (10 ...
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bioRxiv - Cancer Biology 2021Quote: ... GTP-pulldown and western blot procedures were performed according to RHOA/RAC1/CDC42 Activation Assay Combo kit (Cytoskeleton) manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037, Cytoskeleton Inc.).
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: Globular and filamentous actin ratios were determined by western blot as described by commercially available G-actin/ F-actin In Vivo Assay Kit (Cytoskeleton). Globular and filamentous immunocytochemistry was performed as previously described [53] ...
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bioRxiv - Neuroscience 2021Quote: ... washed and the immunoprecipitated resolved on 12% SDS-PAGE and detected via Western blot analysis using Cdc42 (mouse anti-Cdc42, ACD03 Cytoskeleton; 1:250), Rac1 (mouse anti-Rac1 ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were resolved on 12% SDS-PAGE and detected via Western blot analysis using actin (rabbit anti-actin, BK037 Cytoskeleton Inc.; 1:500) and visualized by ECL (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... BlastR™ Lysis Buffer (# BLST01, Cytoskeleton) with 1x Protease inhibitor cocktail (PIC02 ...
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bioRxiv - Cell Biology 2019Quote: ... in G-PEM buffer (Cytoskeleton Inc. BST01). Unpolymerized tubulin ...
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bioRxiv - Neuroscience 2023Quote: ... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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bioRxiv - Biophysics 2021Quote: ... pH 7.5 (actin polymerization buffer, Cytoskeleton, Inc. BSA02) for an hour ...
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bioRxiv - Cancer Biology 2019Quote: ... 15% tubulin glycerol cushion buffer (Cytoskeleton, Inc.; catalog # BPST05-001) and the indicated concentrations of rigosertib (obtained from either Selleckchem [chemical grade] or Onconova Therapeutics ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed in PFA (4% PFA in Cytoskeleton Buffer (CB) – in mM ...
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bioRxiv - Biochemistry 2023Quote: ... 0.2 mM CaCl2 (from General Actin Buffer, cat. BSA01, Cytoskeleton Inc.), 50 mM KCl ...
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bioRxiv - Biophysics 2020Quote: ... 5 μL of 10x polymerization buffer solution (BSA02; Cytoskeleton, Inc., Denver, CO) mixed with 45 μL of the previously produced solution were left for 1h at room temperature to polymerize ...
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bioRxiv - Neuroscience 2021Quote: ... The F-actin pellet was resuspended in a depolymerizing buffer (Cytoskeleton Inc) and then F-and G-actin fractions were diluted in loading buffer (50 mM Tris–HCl ...
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bioRxiv - Biochemistry 2023Quote: ... 2.5 μl tubulin glycerol buffer and 0.75 μl 20 mM GTP (Cytoskeleton), the mixture was incubated at 37 ° C for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... cells were pre-extracted for 5 minutes with cold CSK buffer (‘Cytoskeleton buffer’ ...
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bioRxiv - Genetics 2022Quote: ... general actin buffer (5mM Tris-HCl pH 8.0 and 0.2mM CaCl2, Cytoskeleton) were incubated with actin protein (1mM ...
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bioRxiv - Genetics 2023Quote: ... and actin polymerization buffer are provided by Cytoskeleton (Cytoskeleton, catalogue no. BK003).
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bioRxiv - Neuroscience 2021Quote: ... F-actin pellet was resuspended in a depolymerizing buffer (BK037, Cytoskeleton Inc., USA) and then F- and G-actin fractions were diluted in loading buffer (50 mM Tris–HCl ...
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bioRxiv - Biophysics 2021Quote: ... the buffer also contained 50 µM non-muscle human actin protein (Cytoskeleton, Inc) and 0.2 mM ATP and 0.2 mM CaCl2.
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bioRxiv - Cell Biology 2019Quote: Cells were collected in lysis and F-actin stabilization buffer (LAS01, Cytoskeleton Inc) at 37°C in the presence of Halt Protease Inhibitor Cocktail (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stored in G-LISA lysis buffer with manufacturer’s protease inhibitors (Cytoskeleton). Protein concentrations were determined using Precision Red Advanced Protein Assay Reagent with absorbance at 600 nm measured spectrophotometer ...
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bioRxiv - Microbiology 2022Quote: ... around 3× 107 trophozoites were incubated in cell lysis buffer (Cytoskeleton Inc, Colorado, USA) with vigorous agitation at 4°C for 30 min and homogenized by a 23-gauge needle on a 5-ml syringe ...
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bioRxiv - Cell Biology 2022Quote: ... or TMR-labeled tubulin (1.25 ng in 0.5 nL General Tubulin Buffer, Cytoskeleton, TL590M) was injected with UtrCH-sfGFP and JF646 LANA ...
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bioRxiv - Biophysics 2020Quote: ... were resuspended to using 250 μL of General Actin Buffer (BSA01; Cytoskeleton, Inc., Denver, CO) containing 0.2 mM ATP (New England Biolabs Inc. ...
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bioRxiv - Cell Biology 2019Quote: ... and 100 nM Actistain conjugated to AlexaFluor 555 (in blocking buffer; catalog no. PHDH1, Cytoskeleton), followed by three washes with PBS ...
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bioRxiv - Cell Biology 2023Quote: Stock actin (10 mg/ml) was diluted 1:10 into ATP-supplemented general actin buffer from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) supplemented with 4.51% of sucrose to adjust osmotic pressure (Cytoskeleton buffer with sucrose; CBS) and then fixed in 3% PFA in CBS for 15 min ...
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bioRxiv - Cancer Biology 2019Quote: ... in general tubulin buffer (80mM PIPES, pH 6.9/ 2mM MgCl2/ 0.5mM EGTA/10μM fluorescence reporter) (catalog # BP01; Cytoskeleton, Inc.) was combined with 1mM GTP ...
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bioRxiv - Cell Biology 2022Quote: ... Actin was then polymerized either alone or in the presence of GJA1-20k (final concentration 0.1mM) in 0.25x polymerization buffer (Cytoskeleton). For the depolymerization assay ...
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bioRxiv - Microbiology 2023Quote: ... the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (PHDR1) (1:500) (Cytoskeleton, Inc.) for 30 minutes in the dark at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were then stimulated with vehicle or 100ng/ml EGF for 15 minutes or 2 hours and lysed with Cell Lysis Buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) (Cytoskeleton Inc.) containing protease and phoshatase inhibitors (Pierce/Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μl of porcine tubulin (5mg/ml) were mixed with 0.25 μl of TMR labelled tubulin (5mg/ml, in general tubulin buffer, vendor: Cytoskeleton) and incubated for 5 min on ice ...
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bioRxiv - Microbiology 2022Quote: Proteins were collected in cell lysis buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) supplemented with 1X Protease inhibitor cocktail (Cytoskeleton, PIC02). Lysates were cleared by 1min centrifugation at 10,000g at 4°C and the collected supernatants were snap-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... of each was incubated for 15 min at 37°C in General Tubulin Buffer with 2.25 mg/mL of 99% pure porcine tubulin and 1 mM GTP (all from Cytoskeleton Inc.), in a final volume of 50 μL ...
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bioRxiv - Microbiology 2019Quote: ... Whole colonic tissue was homogenized in cell lysis buffer and protein concentration was determined using the Precision Red Protein Assay (Cytoskeleton). Samples were sent to Eve Technologies for analysis using their 32-multiplex Mouse Cytokine/Chemokine Array.
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1xPBS and lysed in appropriate volume of lysis and F-actin stabilization buffer supplemented with 1mM ATP and protease inhibitor mixture for F-actin stabilization (Cytoskeleton). Lysates were centrifuged at 100 000 x g at 37°C for 1 h to separate the G-actin fraction in the supernatants and pelleted F- actin ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were lysed in 250 µl cell lysis buffer supplemented with protease inhibitors and subjected to a pull-down assay for activated Rac1 (#BK035, Cytoskeleton). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... was added to either supplemented actin buffer alone or in the presence of 0.4 mg mL-1 G-actin (cat. AKL99, Cytoskeleton Inc.). The final ligand concentration was 2.5 μM ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM guanidine carbonate and 1.2 mM ATP (from Actin Polymerization Buffer, cat. BSA02, and ATP, cat. BSA04, Cytoskeleton Inc.). Samples were incubated at 37 °C for 2 h.
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was carefully removed and the pellet was resuspended in a volume of F-actin depolymerization buffer (FAD-02, Cytoskeleton Inc.) matching the volume of the supernatant ...
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bioRxiv - Microbiology 2022Quote: ... 110 μl of LAS2 buffer was prepared according to the protocol of a G-actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) and mixed with the pellet ...
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bioRxiv - Neuroscience 2019Quote: ... cells were rinsed with warmed PBS and cell lysates prepared in cell lysis buffer as instructed and provided by the RhoA or Rac1 pull-down instruction manual and kit (Cytoskeleton, Inc. Denver, CO). Protein quantitation was performed by BCA method and 600 μg of protein was incubated with 50 μg Rhotekin-RBD beads for RhoA pull-down or with 10 µg of PAK-PBD beads for Rac1 pull-down at 4°C for 1 h ...