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Citations for Cytoskeleton :
1 - 50 of 136 citations for Recombinant Mouse Pcsk9 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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bioRxiv - Plant Biology 2019Quote: ... and GST-tagged VCA domain of human WASP protein (Cytoskeleton) were used to initiate actin nucleation ...
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bioRxiv - Cell Biology 2022Quote: ... or along with the human recombinant proteins: WASP-VCA domain protein (Cat# VCG03, Cytoskeleton) or ERK3 protein (M31-34G ...
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bioRxiv - Plant Biology 2019Quote: The VCA domain of human WASP protein was expressed in a bacterial expression system as a GST-tagged fusion protein (Cytoskeleton). The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton) ...
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bioRxiv - Molecular Biology 2023Quote: ... biotinylated tubulin (Cytoskeleton) and 6.5 μL of 13 mg/mL unlabelled pig tubulin13 in BRB80 buffer (80 mM PIPES pH 6.8 ...
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bioRxiv - Biophysics 2024Quote: ... GST-tagged WCA domain of human WASP protein (GST-WCA) was purchased from Cytoskeleton (Cat. #VCG03-A) and used without further purification ...
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bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
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bioRxiv - Biophysics 2020Quote: For preparation of biotinylated microtubules isolated tubulin was mixed with biotinylated tubulin (Cytoskeleton Inc., T333P) at 50:1 mass ratio ...
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bioRxiv - Biochemistry 2022Quote: ... Biotinylated tubulin was obtained from Cytoskeleton.
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bioRxiv - Microbiology 2024Quote: ... 4 μg of biotinylated tubulin (Cytoskeleton) and 100 μg of unlabelled pig tubulin in BRB80 buffer (80 mM PIPES pH 6.8 ...
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bioRxiv - Cell Biology 2021Quote: ... consisting of 6% biotinylated-tubulin (Cytoskeleton Inc) and 6% fluorescent (X-Rhodamine or HiLyte647 ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants were incubated with biotinylated-actin (Cytoskeleton, Inc), previously prepared as indicated by the manufacturer’s ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μl biotinylated tubulin (2 mg/ml, Cytoskeleton) and 7 μl unlabelled pig tubulin (Schlager et al. ...
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bioRxiv - Biophysics 2023Quote: ... 0.3 nM TMR-phalloidin-labeled biotinylated actin (Cytoskeleton) filaments ...
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bioRxiv - Biochemistry 2023Quote: ... Lyophilized biotinylated porcine tubulin was purchased (Cytoskeleton; T333P) and used at a ratio of 1% for generating biotinylated GMPCPP microtubule seeds.
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bioRxiv - Biophysics 2021Quote: ... G-actin and biotinylated actin were bought from Cytoskeleton, Inc ...
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bioRxiv - Biophysics 2021Quote: ... 5 μM F-actin (mixture 20% Biotinylated actin (Cytoskeleton Inc.)) was added to the mixture and incubated at RT for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2020Quote: ... HiLyte-647 tubulin and biotinylated tubulin were all purchased from Cytoskeleton. GMPCPP-stabilised microtubules were polymerised by resuspending 1 μl aliquots of HiLyte-647 labelled tubulin mixes (60% unlabelled tubulin ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μL of 1 mg/mL biotinylated tubulin (Cytoskeleton Inc., #T333P), and 2 μL of 10 mg/mL unlabeled tubulin (Cytoskeleton Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... rhodamine- and Hi-Lyte647-labelled porcine tubulin were purchased from Cytoskeleton (T240 ...
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bioRxiv - Cancer Biology 2023Quote: ... NCI-H295R cells were fluorescently tagged using MemGlow 560 (Cytoskeleton, Inc., Denver, CO) following manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the purified His-MBP-NRIPs were incubated with F-actin (AD99, Cytoskeleton) in 1 ml of IP buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2019Quote: ... biotinylated actin and Alexa-488-labeled actin were purchased from Cytoskeleton (AKL99, AB07) and Thermofisher (A12373 ...
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bioRxiv - Biochemistry 2024Quote: ... Taxol-stabilized human unmodified microtubules assembled with 1.5% biotinylated tubulin (Cytoskeleton, Inc. #T333P) and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton ...
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bioRxiv - Biochemistry 2024Quote: ... filaments of 0.3 nM TMR-phalloidin-labelled biotinylated actin (Cytoskeleton, Denver, CO, USA), 0.018 mg/mL catalase ...
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bioRxiv - Biochemistry 2019Quote: ... The final assay solution contained pM concentrations of 10 - 15% biotinylated actin (Cytoskeleton Inc,) from rabbit skeletal muscle stabilized by rhodamine phalloidin ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.1 - 0.25 nM rabbit skeletal muscle actin polymerized with 15% biotinylated actin (Cytoskeleton) stabilized by rhodamine-phalloidin (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Solutions of 434 nM His-CLASP2α or 1 μM α-actinin (Cytoskeleton, Inc.) were incubated with and without 15 μM phalloidin-stabilized F-actin for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... and with different recombinant Cdc42 (3µg of human Cdc42, #CD01 Cytoskeleton; 3µg of recombinant MBP tagged-C ...
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bioRxiv - Biophysics 2020Quote: ... and 0.1-0.25 nM rabbit skeletal actin filaments polymerized with 10-15% biotinylated actin (Cytoskeleton) and stabilized by rhodamine-phalloidin (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... taxol-stabilized microtubules and recombinant human kinesin (KIF5A) were purchased from Cytoskeleton™ (Denver ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μm carboxylate beads (01-02-103, micromer) were coated with biotinylated laminin (LMN03, Cytoskeleton, Inc.) or FN7-10 (a four-domain segment of fibronectin responsible for cell binding and containing the RGD and PHSRN motifs102 ...
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bioRxiv - Biochemistry 2021Quote: ... a solution containing filaments of 0.3 nM TMR-phalloidin-labeled biotinylated actin (Cytoskeleton, Denver, CO, USA), 0.4% glucose ...
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bioRxiv - Cell Biology 2020Quote: To analyze the competition between actin and His-Rap1b (Cytoskeleton Inc, cat. no. RR02-A) biding to GST SPN some modifications were made to the assay ...
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bioRxiv - Biophysics 2022Quote: ... were cultured on fiber scaffolds (for training data set generation, treatment with rhodamine tagged fibronectin (Cytoskeleton Inc, CO)) in 5% CO2 and 37°C ...
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bioRxiv - Neuroscience 2023Quote: ∼30µg of purified recombinant TDP-43 and 10µg of rabbit skeletal muscle actin (Cytoskeleton, #BK037) were incubated with Ni-NTA beads at 4°C overnight on a rotating wheel ...
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bioRxiv - Cell Biology 2022Quote: ... Cdc42 and ARP3 proteins or ARP2/3 protein complex (Cytoskeleton) were used as bait and full-length GST-ERK3 (SignalChem ...
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bioRxiv - Bioengineering 2022Quote: ... The beads were cleaned 3 times in PBS and resuspended in a solution of PBS with 20 μg/ml biotinylated fibronectin (Cytoskeleton) or biotinylated BSA (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... Seed mixture was prepared as 26 μM of porcine brain tubulin containing 10% biotinylated tubulin and 10% HiLyte 488 tubulin (both from Cytoskeleton) in 1 mM GMPCPP in KPEM ...
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bioRxiv - Cell Biology 2023Quote: Stable HeLa cells that had been induced to express GFP-tagged ESCO1 transgenes for 24 hours were incubated with 250nM SIR-DNA (Cytoskeleton Inc.) and 2µg/ml doxycycline in Opti-MEM (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein quantification was performed using Precision Red Protein Assay Reagent (Cytoskeleton) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... GMPCPP-stabilized, biotinylated microtubule seeds were assembled from bovine brain tubulin (Castoldi & Popov, 2003) and porcine biotin tubulin (Cytoskeleton, Cat #T333), and incubated on the coverslip for 5 min before washing with growth buffer (1 mM GTP in BRB80 80 mM PIPES ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration was determined using Precision Red Advanced Protein Assay (Cytoskeleton, Inc.). 20ug protein were loaded onto 8-12% SDS-PAGE gels and transferred onto nitrocellulose membranes ...
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bioRxiv - Biochemistry 2022Quote: ... The dGAE protein concentration was measured using Advanced Protein Assay Reagent (Cytoskeleton, Inc.) with bovine serum albumin as a standard ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the Precision Red Advanced Protein Assay Reagent (Cytoskeleton ADV02). Extracted proteins (25-35 μg ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentration determined using the Precision Red™ Advanced Protein Assay Reagent (Cytoskeleton). Respective samples were treated with 300nM Nocodazole for 15 min before lysis ...
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bioRxiv - Cell Biology 2020Quote: Purified actin protein (Cytoskeleton, Inc.) was used for the in vitro actin polymerization reaction ...
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bioRxiv - Neuroscience 2020Quote: ... and protein concentration estimated in the supernatant using Precision Red Advanced Protein Assay (Cytoskeleton, CA). Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad) ...
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bioRxiv - Immunology 2023Quote: ... Protein concentrations of crude extracts were determined using the Advanced Protein Assay (Cytoskeleton, Denver, USA).