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Citations for Cytoskeleton :
1 - 47 of 47 citations for Recombinant Mouse Map2k4 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... and GST-tagged VCA domain of human WASP protein (Cytoskeleton) were used to initiate actin nucleation ...
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bioRxiv - Biophysics 2024Quote: ... GST-tagged WCA domain of human WASP protein (GST-WCA) was purchased from Cytoskeleton (Cat. #VCG03-A) and used without further purification ...
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bioRxiv - Plant Biology 2019Quote: The VCA domain of human WASP protein was expressed in a bacterial expression system as a GST-tagged fusion protein (Cytoskeleton). The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton) ...
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bioRxiv - Neuroscience 2023Quote: ... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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bioRxiv - Microbiology 2021Quote: ... Analysis of activated RhoA-GST was performed using GST-Rhotekin-RBD from Cytoskeleton Inc(70).
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bioRxiv - Cell Biology 2022Quote: PAK-GST-coated beads (Cytoskeleton BK035) and Rhotekin-RBD-coated beads(Cytoskeleton BK036 ...
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bioRxiv - Cell Biology 2020Quote: ... and GST-WASP-VCA (Cytoskeleton, Inc. VCG03-A) were reconstituted and used as described in manufacture’s protocols except for size exclusion chromatography (GE Superdex 200 increase 10/300 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and GST-WASP-VCA (VCG03-A) were purchased from Cytoskeleton. ATP for actin dialysis buffer was purchased from Gold Biotechnology ...
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bioRxiv - Plant Biology 2020Quote: ... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
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Mapping chromatin state and transcriptional response in CIC-DUX4 undifferentiated round cell sarcomabioRxiv - Cancer Biology 2023Quote: The RAS GST-RBD activation kit was obtained from Cytoskeleton (Denver, CO, USA; cat #BK008). The protocol was performed following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
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bioRxiv - Biochemistry 2023Quote: ... rhodamine- and Hi-Lyte647-labelled porcine tubulin were purchased from Cytoskeleton (T240 ...
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bioRxiv - Cancer Biology 2023Quote: ... NCI-H295R cells were fluorescently tagged using MemGlow 560 (Cytoskeleton, Inc., Denver, CO) following manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the purified His-MBP-NRIPs were incubated with F-actin (AD99, Cytoskeleton) in 1 ml of IP buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... Solutions of 434 nM His-CLASP2α or 1 μM α-actinin (Cytoskeleton, Inc.) were incubated with and without 15 μM phalloidin-stabilized F-actin for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... and with different recombinant Cdc42 (3µg of human Cdc42, #CD01 Cytoskeleton; 3µg of recombinant MBP tagged-C ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then incubated with 30 µg of Ras-GTP Binding Domain (RBD of human c-Raf kinase) fused with GST associated with beads (#RF02, Cytoskeleton) at 4°C for 1h ...
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bioRxiv - Cell Biology 2022Quote: ... taxol-stabilized microtubules and recombinant human kinesin (KIF5A) were purchased from Cytoskeleton™ (Denver ...
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bioRxiv - Cell Biology 2020Quote: To analyze the competition between actin and His-Rap1b (Cytoskeleton Inc, cat. no. RR02-A) biding to GST SPN some modifications were made to the assay ...
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bioRxiv - Biophysics 2022Quote: ... were cultured on fiber scaffolds (for training data set generation, treatment with rhodamine tagged fibronectin (Cytoskeleton Inc, CO)) in 5% CO2 and 37°C ...
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bioRxiv - Cell Biology 2019Quote: Active Rac levels were determined by pull-down assay using GST-PAK-CRIB immobilised to Glutathione beads according to manufacturer’s instructions (Cytoskeleton, Inc, Active Rac Kit). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... or along with the human recombinant proteins: WASP-VCA domain protein (Cat# VCG03, Cytoskeleton) or ERK3 protein (M31-34G ...
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bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
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bioRxiv - Neuroscience 2023Quote: ∼30µg of purified recombinant TDP-43 and 10µg of rabbit skeletal muscle actin (Cytoskeleton, #BK037) were incubated with Ni-NTA beads at 4°C overnight on a rotating wheel ...
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bioRxiv - Cell Biology 2023Quote: Stable HeLa cells that had been induced to express GFP-tagged ESCO1 transgenes for 24 hours were incubated with 250nM SIR-DNA (Cytoskeleton Inc.) and 2µg/ml doxycycline in Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Rac1 (Cytoskeleton.com; ARC03; mouse monoclonal), Cdc42 (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: ... recombinant human cofilin-1 (CFL1 P23528) and arp2/3 complex (ARPC4 Q148J6) from bovine brain were all purchased from Cytoskeleton Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-RHOA (Cytoskeleton #ARH05, 1:500); rabbit anti-ROCK2 (Cell Signaling #9029 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), Guinea Pig anti-SUMO2/3 (1:300 ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), and Guinea Pig anti-SUMO2/3 (1:300 ...
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bioRxiv - Immunology 2021Quote: ... Mouse monoclonal anti-CDC42 (Cytoskeleton, 1:1000), mouse polyclonal GAPDH (FUJIFILM Wako ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-RhoA (Cytoskeleton ARH05, 1:500); Mouse anti-Rac1 (Cy-toskeleton ARC03 ...
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bioRxiv - Neuroscience 2021Quote: ... Rac1 (mouse anti-Rac1, ARC03 Cytoskeleton; 1:500) and RhoA (mouse anti-RhoA ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was used at 1:500 dilution in TBST overnight.
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was obtained through the Cytoskeleton inc Rho A activation Assay Biochem Kit and used at 1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and RhoA (mouse anti-RhoA, ARH05 Cytoskeleton; 1:500), and visualized by ECL (Pierce ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-ubiquitin (P4D1, Cytoskeleton Inc., IF: 1:250), chicken anti-vimentin (AB5733 ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Cdc42 monoclonal antibody (1:250, Cytoskeleton, Cat. # ACD03), anti-Rac1 (1:250 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-CDC42 (Cytoskeleton Inc., ACD03, 1:250 for Western blot); mouse monoclonal anti-Rac1 (Merck Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used were as follows: mouse anti-RAC1 (Cytoskeleton #ARC03, 1:250); mouse anti-RHOA (Cytoskeleton #ARH05 ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used were mouse anti-Rac1 (1:500, Cytoskeleton Inc, #ARC03) and rabbit anti-GAPDH (1:3000 ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with monoclonal mouse anti-human RhoA primary antibody (# GL01A; 1:250; Cytoskeleton Inc.), followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02 ...
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bioRxiv - Cell Biology 2019Quote: ... followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02; 1:62.5; Cytoskeleton Inc.) on a shaker at 300 rpm at room temperature ...
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bioRxiv - Cell Biology 2020Quote: We used the following monoclonal antibodies: mouse anti-ubiquitin clone P4D1 (Cytoskeleton, Inc, Denver, Co, USA), mouse anti-β-actin clone BA3R (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... The membrane was then probed with a mouse monoclonal anti-RhoA antibody (supplied with the Cytoskeleton Kit) at a working dilution of 1:500 overnight at 4 °C with constant agitation ...
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bioRxiv - Neuroscience 2021Quote: ... washed and the immunoprecipitated resolved on 12% SDS-PAGE and detected via Western blot analysis using Cdc42 (mouse anti-Cdc42, ACD03 Cytoskeleton; 1:250), Rac1 (mouse anti-Rac1 ...
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bioRxiv - Physiology 2020Quote: Recombinant tau from bacteria or heat-stable tau fractions isolated from mouse brains or SH-SY5Y cells stably expressing tau were incubated with 30 μM porcine brain tubulin (Cytoskeleton, Denver, CO, USA) in PEM buffer (80 mM PIPES ...