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Citations for Cytoskeleton :
1 - 50 of 234 citations for Polybrominated Diphenyl Ether Predominant Congener Mixture 1 100 Dilution Unlabeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: A mixture of unlabeled porcine brain tubulin (Cytoskeleton, Inc. Cat. No. T240) was copolymerized with either rhodamine-labelled tubulin (Cytoskeleton ...
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bioRxiv - Biophysics 2022Quote: ... 75 μM unlabeled tubulin (Cytoskeleton) and 5 μM tubulin-AlexaFluor647 (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μM unlabeled porcine tubulin (Cytoskeleton) prepared in binding buffer containing 10 mM DTT and 0.2 mM PMSF was added and incubated overnight on a shaker in a cold room ...
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bioRxiv - Cell Biology 2023Quote: ... and 90% unlabeled tubulin (Cytoskeleton, T240) in BRB80 buffer (80 mM PIPES ...
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bioRxiv - Cell Biology 2021Quote: ... and unlabeled G-actin (AKL99, Cytoskeleton Inc) successively into the assembly buffer and let them equilibrate at 37 °C for 1 hour ...
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bioRxiv - Biophysics 2021Quote: ... 20 µM unlabeled porcine brain tubulin (Cytoskeleton #T240) was polymerized at 37°C for 2 hours in BRB80 (80mM PIPES pH 6.8 ...
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Cell Biology 2021Quote: Microtubules were prepared using unlabeled porcine brain tubulin (Cytoskeleton) reconstituted in ice-cold BRB80 buffer to 5 mg/ml and polymerized in the presence of 1mM Mg-GTP for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 2 µL of 10 mg/mL unlabeled tubulin (Cytoskeleton) was mixed with 2 µL o f 1 mg/mL biotin-tubulin and 2 µL of 1 mg/mL dye-labeled tubulin on ice ...
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bioRxiv - Biochemistry 2023Quote: ... Taxol-stabilized microtubules assembled from unlabeled porcine tubulin (Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Cell Biology 2022Quote: ... MT was polymerized by mixing unlabeled bovine brain tubulin (Cytoskeleton) and rhodamine-tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2019Quote: ... MTs were made by incubating 77% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Developmental Biology 2023Quote: ... The explants were then incubated overnight with a 1:100 dilution of Alexa488 Acti-stain (Cytoskeleton PHDG1) and imaged using a Nikon AX confocal microscope and a10x objective.
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bioRxiv - Cell Biology 2021Quote: GMPCPP-stabilized microtubules were assembled using 50 μM unlabeled porcine tubulin containing 1:12 biotin-labeled porcine tubulin (Cytoskeleton) and polymerized in the presence of 1mM GMPCPP (Jena Bioscience ...
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bioRxiv - Biophysics 2019Quote: ... by incubating the tubulin mix containing 70% unlabeled porcine brain tubulin (Cytoskeleton), 18% biotin-tubulin (Cytoskeleton ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 μL of 10 mg/mL unlabeled tubulin (Cytoskeleton Inc., #T240) was mixed on ice ...
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bioRxiv - Biochemistry 2021Quote: Porcine Tubulin at 1:5 ratio (rhodamine-labeled: unlabeled) (cat. no. T240 and TL590M-A respectively, Cytoskeleton, Inc., Denver, USA) was reconstituted to 4.0 mg/mL in cold polymerization buffer ...
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bioRxiv - Microbiology 2021Quote: Rabbit skeletal muscle actin was purchased (unlabeled and pyrene-labeled actin; Cytoskeleton Inc.). All actin was maintained in G buffer (5 mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... GMPCPP MT seeds were prepared by incubating 77% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Cell Biology 2022Quote: Microtubules were polymerized (purified porcine tubulin unlabeled and HiLyte-647-labeled, Cytoskeleton Inc) in BRB80 buffer [80 mM Pipes/KOH pH 6.8 ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μM rabbit skeletal muscle actin (90% unlabeled and 10% rhodomine-actin) (Cytoskeleton Inc.), 1 μM Shi were incubated in 6.5 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: Unlabeled and labeled (biotin and TRITC) lyophilized tubulin from porcine brain were purchased from Cytoskeleton Inc (Denver ...
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bioRxiv - Cell Biology 2019Quote: ... Actin filaments (28 μM) were made by incubating 83% unlabeled G-actin (APHL99; Cytoskeleton Inc.) with 8.5% biotin-G-actin (AB07 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-SUMO2/3 (Asm23, Cytoskeleton, 1:200 dilution), and anti-PML (sc966 ...
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bioRxiv - Cell Biology 2021Quote: Microtubules were polymerized (unlabeled and HiLyte-647-labeled porcine brain tubulin, Cytoskeleton Inc #T240 and #TL670M) in BRB80 buffer (80 mM Pipes/KOH pH 6.8 ...
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bioRxiv - Cell Biology 2021Quote: ... Different types of labelled and unlabeled purified tubulin used in the assays were either purchased from Cytoskeleton or purified as described previously (57 ...
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bioRxiv - Cell Biology 2019Quote: ... GMPCPP MT bright seeds were prepared as described above by incubating 66.7% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Cell Biology 2019Quote: ... First double cycled GMPCPP MT seeds were prepared by incubating tubulin mix containing 70% unlabeled porcine brain tubulin (Cytoskeleton), 18 % biotin-tubulin (Cytoskeleton ...
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bioRxiv - Biophysics 2020Quote: ... 10% rhodamine-labeled F-actin was prepared by co-polymerizing 0.9 μM unlabeled actin monomers and 0.1 μM rhodamine-labeled actin monomers (Cytoskeleton, AR05) in the presence of G-Mg (2 mM Tris-HCl pH 8.0 ...
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Trypanosoma brucei colonises the tsetse gut via an immature peritrophic matrix in the proventriculusbioRxiv - Microbiology 2019Quote: ... stained with SiR-actin (1:1000 dilution in PBS, Cytoskeleton Inc.) for 4 hours ...
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bioRxiv - Immunology 2021Quote: ... or acti-stain 488 phalloidin (Cytoskeleton, Inc, at 1:75 dilution) in the blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... anti-alpha/beta tubulin (1:100; Cytoskeleton). F(ab’)2 fragment affinity-purified secondary antibodies (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... incubated with rhodamine phalloidin (1:100) (Cytoskeleton Inc.) for 30 minutes at room temperature in a humidified ...
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bioRxiv - Cell Biology 2021Quote: ... MT affinity/dead-head spin was performed by binding motor protein to newly polymerized MTs (unlabeled, 2uM Paclitaxel (Taxol (Cytoskeleton, Denver, CO, US, TXD01) -stabilized ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with a 1:1000 dilution of SPY555-Actin Probe (CY-SC202, Cytoskeleton) in complete DMEM at 37°C for three hours prior to imaging ...
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bioRxiv - Developmental Biology 2024Quote: ... Cysts were incubated in a culture medium containing SPY650-actin (Cytoskeleton, Inc., 1:2000 dilution) for 3 h before indentation ...
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bioRxiv - Biochemistry 2020Quote: ... Fixed tissues were washed in PBS (and stained with SiR-actin (1:1000 dilution, Cytoskeleton Inc.) for 3 hours at room temperature (RT ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... actin filaments (Acti-stain 670 phalloidin, 1:600 dilution; Cat: SKU PHDN1, Cytoskeleton, Denver, CO USA). Imaging was performed using a spinning disk confocal microscope (Yokogawa CSU-X1 ...
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bioRxiv - Biophysics 2021Quote: ... 5 μM F-actin (mixture 20% Biotinylated actin (Cytoskeleton Inc.)) was added to the mixture and incubated at RT for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with 1:100 rhodamine phalloidin (# PHDR1, Cytoskeleton Inc.) for 60 min at room temperature.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were stained in suspension with a 1:300 dilution of Anti-Stain 488 Fluorescent Phalloidin (Cytoskeleton, #PHDG1), gently mixed ...
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bioRxiv - Microbiology 2022Quote: ... 2% BSA) prior to immunostaining for 20 min with a 1:500 dilution of Acti-stain-555 (Cytoskeleton Inc). The ratio of cytoplasmic actin to cortical actin was measured using ImageJ (Fiji 2.1.0/1.53c ...
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bioRxiv - Cell Biology 2019Quote: ... The PDMS stamps were inked either with 100 μg/ml 1:1 ratio of rhodamine-conjugated fibronectin (Cytoskeleton FNR01) and non-labelled fibronectin (Sigma-Aldrich F2006) ...
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bioRxiv - Bioengineering 2019Quote: HUVECs were seeded on the PEG islands and control SiO2 substrates for 3 h and then flooded with cell culture medium containing 1:1000 dilution of SiR-DNA (Cytoskeleton Inc). An additional hour was required for cells to uptake SiR-DNA to be trackable through SiR-DNA probe using Leica DMI6000 microscope ...
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bioRxiv - Cell Biology 2023Quote: ... Polymerization assays were performed using a mixture of commercial rhodamine labeled actin monomers (Cytoskeleton, Inc.) and unlabeled actin monomers that were mixed at a 1:1 ratio ...
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bioRxiv - Cell Biology 2023Quote: ... rhodamine-labeled MTs were polymerized using a mixture containing 5% rhodamine-labeled tubulin (Cytoskeleton, TL590M), 5% biotin-labeled tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1xPBS and lysed in appropriate volume of lysis and F-actin stabilization buffer supplemented with 1mM ATP and protease inhibitor mixture for F-actin stabilization (Cytoskeleton). Lysates were centrifuged at 100 000 x g at 37°C for 1 h to separate the G-actin fraction in the supernatants and pelleted F- actin ...
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bioRxiv - Cell Biology 2022Quote: ... and 100 nM SiR-DNA (Cytoskeleton inc. ...
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bioRxiv - Cell Biology 2022Quote: ... was added to start the assay at a final concentration of 1μM and the mixture was added to flow chambers that were preincubated with N-Ethylmaleimide (NEM)-Heavy-Mero-Myosin II (Cytoskeleton, Inc.) and washed with 1% bovine serum albumin (Thermo Fisher Scientific ...