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Citations for Cytoskeleton :
1 - 50 of 152 citations for Neural Precursor Cell Expressed Developmentally Down Regulated 9 NEDD9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... GTP-bound form of CDC42 was pulled down for analysis using the Cdc42 Pull-Down Activation Assay Kit (Cytoskeleton). FLAG-tagged protein pull-down assays were performed using DDDDK tagged protein purification kit (MBL) ...
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bioRxiv - Cell Biology 2024Quote: ... and pten– cells was assessed using a pull-down assay kit (Cat. #BK008-S; Cytoskeleton, Denver, CO) following the manufacturer’s instructions as previously described (Kirolos and Gomer ...
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bioRxiv - Cancer Biology 2024Quote: ... The RhoActivity assay was performed using the RhoA pull-down activation assay biochem kit (Bead pull-down format) (Cytoskeleton, Cat No. BK036). The assay was carried out by incubating 800 µg of total cell lysate with 50 µg of Rhotekin as described in the manufacturers protocol following which western blots were performed ...
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bioRxiv - Cancer Biology 2022Quote: ... the RAS-GTP pull-down assay kit (Cytoskeleton #BK008) was used ...
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bioRxiv - Cell Biology 2021Quote: ... cells lysates were prepared according to manufacturer’s instructions (RhoA Pull-Down Activation Assay Biochem Kit; Cytoskeleton Inc., BK036). Specifically ...
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bioRxiv - Biophysics 2021Quote: ... Washed cells were diluted 1:9 and stained with 50 nM SiR-actin (Spirochrome/Cytoskeleton) for 30 minutes (Lukinavicius et al. ...
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bioRxiv - Systems Biology 2020Quote: ... 300µg of total cell protein was used to pull-down GTP-bound RAS/RAF-RBD complexes according to the manufacturer’s instructions (Cytoskeleton). Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: The RhoA Activation Assay Biochem Kit (bead pull-down format, Cytoskeleton) was used for performing the pull-down assay on 2D monolayers that were either uninduced or induced for the (DN ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were lysed in 250 µl cell lysis buffer supplemented with protease inhibitors and subjected to a pull-down assay for activated Rac1 (#BK035, Cytoskeleton). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: Rho-GTPase pull-down assays were performed following the manufacturer’s protocol (Cytoskeleton, Inc.). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... and the Cdc42 pull-down activation assay kit (#BK034) were purchased from Cytoskeleton, Inc ...
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bioRxiv - Biochemistry 2024Quote: ... we used a MT binding protein spin-down assay kit (# BK029, Cytoskeleton, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... which contained 9% HiLyte-488-tubulin (TL488M; Cytoskeleton Inc.) in BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Neuroscience 2019Quote: ... cells were rinsed with warmed PBS and cell lysates prepared in cell lysis buffer as instructed and provided by the RhoA or Rac1 pull-down instruction manual and kit (Cytoskeleton, Inc. Denver, CO). Protein quantitation was performed by BCA method and 600 μg of protein was incubated with 50 μg Rhotekin-RBD beads for RhoA pull-down or with 10 µg of PAK-PBD beads for Rac1 pull-down at 4°C for 1 h ...
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bioRxiv - Pathology 2023Quote: ... MT assays were performed using the MT Binding Protein Spin-down kit from Cytoskeleton, Inc ...
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bioRxiv - Cancer Biology 2022Quote: We performed Rac1–GTP pull-down assay using the Rac1 activation kit (# BK035-S, Cytoskeleton) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: The Microtubule Binding Protein Spin-down assay was performed according to the manufacturers’ protocol (Cytoskeleton). Briefly ...
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bioRxiv - Cell Biology 2020Quote: For GTP-RhoA pull down assay the Rho Activation Assay biochem kit (Cytoskeleton inc, Cat# BK036) was used as described in the product manual ...
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bioRxiv - Cell Biology 2020Quote: Rho activation assay was performed using a RhoA Pull-down Activation Assay Biochem Kit from Cytoskeleton Inc (BK036-S) ...
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bioRxiv - Cell Biology 2021Quote: The Rac1 activation assay was conducted using the Rac1 Pull-Down Activation Assay Biochem Kit (Cytoskeleton) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Rac1 activity was determined using the Rac1 Pull-Down Activation Assay Biochem Kit (Cytoskeleton, Inc., BK035), following manufacture’s protocol ...
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bioRxiv - Immunology 2023Quote: ... RhoA GTPase activity was measured using the RhoA Pull-Down Activation Assay Biochem Kit (#BK036; Cytoskeleton) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The microtubules binding experiments were performed with the Microtubule Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton, Inc.) following manufacturer protocol.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Active RhoA levels were measured using the RhoA Pull-down Activation Assay Biochem Kit (BK036-S, Cytoskeleton Inc.) using GST Rhotekin beads ...
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bioRxiv - Cell Biology 2021Quote: Cdc42 and Rac1 Pull-Down assays were carried out using Cdc42/Rac1 Activation Assay Biochem Kits (Cytoskeleton, inc), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Actin bundling assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton Inc.) according to protocol of the manufacturer.
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bioRxiv - Biophysics 2021Quote: ... After a 9 minute incubation with 50 nM SiR-Actin (Cytoskeleton Inc., CY-SC001), the sam-ple was incubated with Prolong Live Antifade Reagent (In-vitrogen P36975 ...
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bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Rac1-GTP and Cdc42-GTP using the RhoA and Cdc42 pull-down activation assay biochem kit (PN:BK036-S and BK034-S, Cytoskeleton, Inc.) following the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: ... In vitro microtubule-binding assay was performed using the microtubule-binding protein spin down assay kit (Cytoskeleton, Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: RhoAGTP and Rac1GTP pull down as-says were performed using the RhoA and Rac1 Activation Assay Biochem Kit (Cytoskeleton BK030) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Activation of ARF6 (ARF6-GTP) was quantitated using the Arf6 Pull-Down Activation Assay Biochem Kit from Cytoskeleton (BK033-S, USA) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: The VCA domain of human WASP protein was expressed in a bacterial expression system as a GST-tagged fusion protein (Cytoskeleton). The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton) ...
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bioRxiv - Neuroscience 2022Quote: Rac1 activation was measured by using a Rac1 activation assay kit (Rac1 Pull-down Activation Assay Biochem Kit bead pulldown format, Cytoskeleton, catalog #BK035), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: Active Rac levels were determined by pull-down assay using GST-PAK-CRIB immobilised to Glutathione beads according to manufacturer’s instructions (Cytoskeleton, Inc, Active Rac Kit). Briefly ...
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bioRxiv - Cell Biology 2022Quote: Guanine nucleotide exchange (GEF) activity of ERK3 (9-327 aa) was further evaluated using RhoGEF Exchange Assay (Cat# BK100, Cytoskeleton). Assay was performed according to the manufactures’s instructions and included Dbs GEF as a positive control.
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were incubated in primary antibody diluted in TBST (1:500 anti-RHOA antibody, Cytoskeleton ARH04) or diluted in block (1:30,000 anti-a-alpha tubulin antibody ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed and sequentially incubated with primary antibodies to RhoA followed by HRP secondary antibodies (Cytoskeleton), and the colorimetric reaction was performed for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was used at 1:500 dilution in TBST overnight.
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was obtained through the Cytoskeleton inc Rho A activation Assay Biochem Kit and used at 1:500 ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal Rac1 antibodies (Cat. # ARC03) were purchased from Cytoskeleton.
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bioRxiv - Immunology 2022Quote: Monoclonal Rac1 antibodies (Cat. # ARC03) were purchased from Cytoskeleton.
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bioRxiv - Molecular Biology 2019Quote: Mouse antibodies against Myc-tag (M185-3L/M047-3, MBL, Nagoya, Japan), rabbit antibody against GFP-tag (D110008, BBI, Shanghai, China) and sheep antibody against Tubulin (ATN02, Cytoskeleton, Denver, United States) were subjected to Western blot according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Primary antibodies used were rabbit anti-actin polyclonal (Cytoskeleton, Denver, CO) and mouse anti–MYO-3 monoclonal (5–6 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with a polyclonal antibody against actin (1:500; Cytoskeleton Inc.) and developed by chemiluminescence ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Cdc42 monoclonal antibody (1:250, Cytoskeleton, Cat. # ACD03), anti-Rac1 (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... and incubation in secondary antibody and Acti-Stain 670 Phalloidin (Cytoskeleton, inc), plus Hoechst (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... moderate amounts of anti-ac-K antibody agarose beads (Cytoskeleton, Inc., USA) were added to the lysate and incubated for 12 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used were as follows: mouse anti-RAC1 (Cytoskeleton #ARC03, 1:250); mouse anti-RHOA (Cytoskeleton #ARH05 ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used: rabbit anti-cofilin (Cytoskeleton ACFL02; 1:500), rabbit anti-Ser3-phosphorylated cofilin (Cell Signaling Technology #3311 ...