Labshake search
Citations for Cytoskeleton :
1 - 50 of 217 citations for Mouse E3 Ubiquitin Protein Ligase SIAH2 SIAH2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... The isolation of ubiquitinated proteins was performed with the Signal-Seeker Ubiquitin Enrichment kit (Cytoskeleton). Cell fractionation was performed with the Qproteome Cell Compartment kit (Qiagen).
-
bioRxiv - Neuroscience 2019Quote: ... mouse anti-ubiquitin (P4D1, Cytoskeleton Inc., IF: 1:250), chicken anti-vimentin (AB5733 ...
-
bioRxiv - Microbiology 2023Quote: ... For ubiquitin pulldown assays using the SignalSeeker kit (Cytoskeleton Inc), cells were lysed in provided BlastR buffer with protease inhibitor and NEM ...
-
bioRxiv - Cell Biology 2020Quote: We used the following monoclonal antibodies: mouse anti-ubiquitin clone P4D1 (Cytoskeleton, Inc, Denver, Co, USA), mouse anti-β-actin clone BA3R (Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2020Quote: ... Ubiquitin (Cytoskeleton Inc., AUB01, 1:40). After washing four times with PBS ...
-
bioRxiv - Physiology 2020Quote: ... Ubiquitin (Cytoskeleton Inc., AUB01, 1:400), CHIP/STUB1 (Santa Cruz Biotechnology ...
-
bioRxiv - Cell Biology 2020Quote: Commercial ELISA kits were purchased from Cytoskeleton (G-LISA) to quantify RhoA and Rac1 activation (Cytoskeleton) ...
-
bioRxiv - Cancer Biology 2022Quote: RAS activation was assessed by ELISA using a commercially available kit (Cytoskeleton: BK131) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed by western blot using anti-ubiquitin antibody (P4D1, Cytoskeleton AUB01-HRP, 1:1000) and visualized with Clarity Western ECL Substrate (BioRad).
-
bioRxiv - Neuroscience 2020Quote: ... and RAC1 were performed through the use of an ELISA-based Rho-GTPase activation assay kit (Cytoskeleton, Inc, catalog# BK124, BK127, BK128). Briefly ...
-
bioRxiv - Biochemistry 2024Quote: ... we used a MT binding protein spin-down assay kit (# BK029, Cytoskeleton, Inc.) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2023Quote: ... MT assays were performed using the MT Binding Protein Spin-down kit from Cytoskeleton, Inc ...
-
bioRxiv - Cell Biology 2023Quote: Actin binding and polymerization were assessed using the actin binding protein kit (Cytoskeleton, BK013). Actin pellet gel bands were quantified by densitometry using FIJI.
-
bioRxiv - Neuroscience 2023Quote: ... or Cdc42 activation in vivo assay biochemical kits for the respective protein (Cytoskeleton Inc., Denver, CO). The tissues were lysed in lysis buffer (50mM Tris-HCl pH7.4 ...
-
bioRxiv - Plant Biology 2020Quote: The actin polymerization modulation activity of the proteins was checked using Actin Polymerization Biochem Kit (Cytoskeleton, USA), using the manufacturer’s instruction ...
-
bioRxiv - Biophysics 2022Quote: ... The membrane was then probed with a mouse monoclonal anti-RhoA antibody (supplied with the Cytoskeleton Kit) at a working dilution of 1:500 overnight at 4 °C with constant agitation ...
-
bioRxiv - Microbiology 2020Quote: The microtubules binding experiments were performed with the Microtubule Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton, Inc.) following manufacturer protocol.
-
bioRxiv - Neuroscience 2023Quote: Actin bundling assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton Inc.) according to protocol of the manufacturer.
-
bioRxiv - Cell Biology 2022Quote: ... Cdc42 and ARP3 proteins or ARP2/3 protein complex (Cytoskeleton) were used as bait and full-length GST-ERK3 (SignalChem ...
-
bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... Protein quantification was performed using Precision Red Protein Assay Reagent (Cytoskeleton) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein concentration was determined using Precision Red Advanced Protein Assay (Cytoskeleton, Inc.). 20ug protein were loaded onto 8-12% SDS-PAGE gels and transferred onto nitrocellulose membranes ...
-
bioRxiv - Neuroscience 2020Quote: ... In vitro microtubule-binding assay was performed using the microtubule-binding protein spin down assay kit (Cytoskeleton, Denver, CO, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... and Rac or Rho activity was assessed in duplicate using the G-LISA small G-protein activation assay kit (BK128, BK124, Cytoskeleton) according to the manufacturer’s directions ...
-
bioRxiv - Biochemistry 2022Quote: ... The dGAE protein concentration was measured using Advanced Protein Assay Reagent (Cytoskeleton, Inc.) with bovine serum albumin as a standard ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the Precision Red Advanced Protein Assay Reagent (Cytoskeleton ADV02). Extracted proteins (25-35 μg ...
-
bioRxiv - Cell Biology 2019Quote: ... and protein concentration determined using the Precision Red™ Advanced Protein Assay Reagent (Cytoskeleton). Respective samples were treated with 300nM Nocodazole for 15 min before lysis ...
-
bioRxiv - Cell Biology 2022Quote: ... or along with the human recombinant proteins: WASP-VCA domain protein (Cat# VCG03, Cytoskeleton) or ERK3 protein (M31-34G ...
-
bioRxiv - Cell Biology 2020Quote: Purified actin protein (Cytoskeleton, Inc.) was used for the in vitro actin polymerization reaction ...
-
bioRxiv - Neuroscience 2020Quote: ... and protein concentration estimated in the supernatant using Precision Red Advanced Protein Assay (Cytoskeleton, CA). Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad) ...
-
bioRxiv - Immunology 2023Quote: ... Protein concentrations of crude extracts were determined using the Advanced Protein Assay (Cytoskeleton, Denver, USA).
-
bioRxiv - Cancer Biology 2022Quote: ... Rac1 (Cytoskeleton.com; ARC03; mouse monoclonal), Cdc42 (Santa Cruz Biotechnology ...
-
bioRxiv - Plant Biology 2019Quote: ... The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton). The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare ...
-
bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were measured using the Precision Red Advanced Protein Assay Reagent (# ADV02; Cytoskeleton Inc.) and finally equalized with ice-cold lysis buffer to 1.0 mg/ml ...
-
bioRxiv - Cell Biology 2019Quote: ... Equalized protein extractions were transferred to a Rho-GTP-binding protein pre-coated plate (Cytoskeleton, Inc.). The plate was placed on a microplate shaker at 300 rpm for 30 min at 4°C ...
-
bioRxiv - Developmental Biology 2019Quote: ... C3 exoenzyme protein (CT03; Cytoskeleton, Inc.) was resuspended and dialyzed into PBS and injected at 1 mg/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein concentration of the cleared lysates was determined by Precision Red Advanced Protein Assay Reagent (Cytoskeleton Inc.). Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen ...
-
bioRxiv - Genetics 2024Quote: ... Protein quantification was determined via Bradford assay using PrecisionRed Advanced Protein Assay reagent (#ADV01, Cytoskeleton, Denver, CO, USA). Lysate concentration was normalized before dilution in 2X Laemmli Sample buffer with 10% v/v 2-mercaptoethanol ...
-
bioRxiv - Cell Biology 2020Quote: Purified tubulin proteins were obtained from Cytoskeleton, Inc ...
-
bioRxiv - Cancer Biology 2021Quote: ... mouse anti-RHOA (Cytoskeleton #ARH05, 1:500); rabbit anti-ROCK2 (Cell Signaling #9029 ...
-
bioRxiv - Cell Biology 2020Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), Guinea Pig anti-SUMO2/3 (1:300 ...
-
bioRxiv - Cell Biology 2019Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), and Guinea Pig anti-SUMO2/3 (1:300 ...
-
bioRxiv - Immunology 2021Quote: ... Mouse monoclonal anti-CDC42 (Cytoskeleton, 1:1000), mouse polyclonal GAPDH (FUJIFILM Wako ...
-
bioRxiv - Cell Biology 2023Quote: ... Mouse anti-RhoA (Cytoskeleton ARH05, 1:500); Mouse anti-Rac1 (Cy-toskeleton ARC03 ...
-
bioRxiv - Plant Biology 2019Quote: The VCA domain of human WASP protein was expressed in a bacterial expression system as a GST-tagged fusion protein (Cytoskeleton). The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton) ...
-
bioRxiv - Microbiology 2019Quote: ... Whole colonic tissue was homogenized in cell lysis buffer and protein concentration was determined using the Precision Red Protein Assay (Cytoskeleton). Samples were sent to Eve Technologies for analysis using their 32-multiplex Mouse Cytokine/Chemokine Array.
-
bioRxiv - Neuroscience 2023Quote: ... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
-
bioRxiv - Molecular Biology 2020Quote: ... 160 nM WASP VCA domain protein (#VCG03, Cytoskeleton) and 60 nM Arp2/3 protein complex (#RP01P ...
-
bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined using Precision Red (Cytoskeleton) and 60μg of protein were separated using 4-15% SDS-PAGE gels and transferred onto nitrocellulose membrane ...
-
bioRxiv - Cancer Biology 2022Quote: ... Precision Red Advanced Protein Assay (ADV02-A; Cytoskeleton), calcein AM (C1430 ...