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Citations for Cytoskeleton :
1 - 50 of 194 citations for Human NADH ubiquinone oxidoreductase chain 5 MT ND5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... MT assays were performed using the MT Binding Protein Spin-down kit from Cytoskeleton, Inc ...
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bioRxiv - Biophysics 2019Quote: MTs were prepared by polymerizing 5 mg/ml tubulin (Cytoskeleton, Denver, CO) in BRB80 buffer (80 mM PIPES ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: MTs were polymerized by incubating 5 µM tubulin (4% HyLite 647 tubulin (Cytoskeleton #TL670M), ∼1% biotin tubulin (Cytoskeleton #T333P) ...
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bioRxiv - Cell Biology 2023Quote: ... rhodamine-labeled MTs were polymerized using a mixture containing 5% rhodamine-labeled tubulin (Cytoskeleton, TL590M), 5% biotin-labeled tubulin (Cytoskeleton ...
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bioRxiv - Biochemistry 2024Quote: ... we used a MT binding protein spin-down assay kit (# BK029, Cytoskeleton, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: MTs were polymerized (tubulin, Cytoskeleton Inc) in BRB80 buffer (80 mM Pipes/KOH pH 6.8 ...
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bioRxiv - Cell Biology 2020Quote: Commercial ELISA kits were purchased from Cytoskeleton (G-LISA) to quantify RhoA and Rac1 activation (Cytoskeleton) ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μM β-actin (human platelets; Cytoskeleton) whose pH was adjusted to 7.25 with KOH ...
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bioRxiv - Cell Biology 2019Quote: MT polymerization assays were performed using a fluorescence-based Tubulin Polymerization kit (Cytoskeleton Inc, Denver CO, Cat. # BK011P) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... MT affinity/dead-head spin was performed by binding motor protein to newly polymerized MTs (unlabeled, 2uM Paclitaxel (Taxol (Cytoskeleton, Denver, CO, US, TXD01) -stabilized ...
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bioRxiv - Cell Biology 2022Quote: ... MT was polymerized by mixing unlabeled bovine brain tubulin (Cytoskeleton) and rhodamine-tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2019Quote: ... MTs were made by incubating 77% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Biochemistry 2023Quote: Microtubules (MTs) were prepared from porcine brain tubulin (Cytoskeleton, Inc. CO). Tubulin lyophilized pellets were resuspended in BRB80 (80 mM K-PIPES ...
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bioRxiv - Cancer Biology 2022Quote: RAS activation was assessed by ELISA using a commercially available kit (Cytoskeleton: BK131) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... GMPCPP MT seeds were prepared by incubating 77% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Biochemistry 2022Quote: ... paclitaxel-stabilised MTs containing 10% X-rhodamine-labelled and 10% biotin-labelled tubulin (Cytoskeleton) were polymerized as above and were left at room temperature for 48 hours before use in the TIRF assay.
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Cell Biology 2019Quote: ... GMPCPP MT bright seeds were prepared as described above by incubating 66.7% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Biophysics 2021Quote: ... Labeled MTs were generated by mixing 4μL of 20 μM Cy5 (PurSolutions) or Rhodamine (Cytoskeleton, Denver, CO) labeled tubulin with 9 μL unlabeled MTs and transferred to a 37°C water bath for 20 min and stabilized by adding 10 μM paclitaxel (Cytoskeleton ...
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bioRxiv - Neuroscience 2021Quote: Effects of tau and nocodazole on MT polymerization were tested using a Tubulin Polymerization Assay (Cytoskeleton Inc., Denver, CO). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... First double cycled GMPCPP MT seeds were prepared by incubating tubulin mix containing 70% unlabeled porcine brain tubulin (Cytoskeleton), 18 % biotin-tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... Seeds were formed by shearing larger MTs (GMPCPP-stabilized, labeled 1:40 with HiLyte 488 tubulin (Cytoskeleton, TL488M-B) with a Hamilton syringe (Avanti Polar Lipids ...
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bioRxiv - Neuroscience 2020Quote: ... and RAC1 were performed through the use of an ELISA-based Rho-GTPase activation assay kit (Cytoskeleton, Inc, catalog# BK124, BK127, BK128). Briefly ...
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bioRxiv - Biophysics 2021Quote: ... labeled tubulin with 9 μL unlabeled MTs and transferred to a 37°C water bath for 20 min and stabilized by adding 10 μM paclitaxel (Cytoskeleton, Denver, CO). MTs were stored at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Human platelet actin (Cytoskeleton Inc.) was polymerized in F-actin buffer (5 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: Human non muscle actin (Cytoskeleton, Inc.) in general actin buffer (5 mM Tris-HCl [pH 8.0] and 0.2 mM CaCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... human platelet actin (Cytoskeleton Inc., APHL99), were mixed in equimolar ratios at 50 μM and incubated on ice for 30 minutes prior to measurement.
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bioRxiv - Cell Biology 2020Quote: Purified human platelet actin (APHL99, Cytoskeleton) was polymerized following the manufacturer’s protocol and attached to polylysine coated coverslips by incubation with 2 µg/ml actin in actin polymerization solution ...
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bioRxiv - Cancer Biology 2023Quote: Human non-muscle actin (Cytoskeleton, # APHL99) was polymerized or de-polymerized according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: Purified (>99%) human actin monomers (Cytoskeleton, APLC99) were prepared (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mL DMSO (Cytoskeleton)] was added to the stock microtubule solution at room temperature.
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bioRxiv - Biophysics 2022Quote: ... Lyophilized human cofilin 1 was purchased from Cytoskeleton (CF01) and reconstituted in MB buffer (20 mM MOPS pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... Human full-length gelsolin was purchased from Cytoskeleton (US).
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bioRxiv - Plant Biology 2019Quote: ... and GST-tagged VCA domain of human WASP protein (Cytoskeleton) were used to initiate actin nucleation ...
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bioRxiv - Biophysics 2022Quote: ... and 5 μM tubulin-AlexaFluor647 (Cytoskeleton) were combined with 1mM DTT and 0.6mM GMP-CPP in M2B buffer and incubated spun at 300,000 g to remove aggregates ...
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bioRxiv - Cell Biology 2023Quote: ... 5% biotin-labeled tubulin (Cytoskeleton, T333P), and 90% unlabeled tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2023Quote: ... and with different recombinant Cdc42 (3µg of human Cdc42, #CD01 Cytoskeleton; 3µg of recombinant MBP tagged-C ...
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bioRxiv - Cell Biology 2022Quote: ... taxol-stabilized microtubules and recombinant human kinesin (KIF5A) were purchased from Cytoskeleton™ (Denver ...
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bioRxiv - Microbiology 2023Quote: ... 5 µg of purified porcine tubulin (Cytoskeleton) was present in protein mixtures prior to Flag Ab immunoprecipitation.
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bioRxiv - Microbiology 2023Quote: Microtubules with ∼5% biotin–tubulin (Cytoskeleton inc.) and ∼5% Alexa488-labeled or Alexa647-labeled fluorescent tubulin (Cytoskeleton inc. ...
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bioRxiv - Biophysics 2021Quote: ... the buffer also contained 50 µM non-muscle human actin protein (Cytoskeleton, Inc) and 0.2 mM ATP and 0.2 mM CaCl2.
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bioRxiv - Biochemistry 2024Quote: ... Taxol-stabilized human unmodified microtubules assembled with 1.5% biotinylated tubulin (Cytoskeleton, Inc. #T333P) and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton ...
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bioRxiv - Neuroscience 2020Quote: ... Non-muscle actin (5 µM, APHL99, Cytoskeleton Inc.) was included in the pipette to prevent wash-out of plasticity63 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μg/mL RhoA Activator II (Cytoskeleton, Inc.) or 2 μg/mL Rho A Inhibitor I (Cytoskeleton ...
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bioRxiv - Cell Biology 2022Quote: ... or along with the human recombinant proteins: WASP-VCA domain protein (Cat# VCG03, Cytoskeleton) or ERK3 protein (M31-34G ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μl of soluble TRITC-labelled tubulin (Cytoskeleton, TL590M) in phase separation buffer containing 500 ng ml−1 nocodazole was added for a final tubulin concentration of 5 μM ...
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bioRxiv - Biophysics 2023Quote: 5 μM porcine brain tubulin (Cytoskeleton, Cat No. T240) and 5 μM MAP7 MTBD were mixed with 2 mM GTP (final concentration ...
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bioRxiv - Biochemistry 2020Quote: ... For measurements including actin (human platelet actin, 99% pure, from Cytoskeleton, Inc., catalog nr: APHL99), the buffer was 5 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... 5 μM F-actin (mixture 20% Biotinylated actin (Cytoskeleton Inc.)) was added to the mixture and incubated at RT for 10 min ...