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Citations for Cytoskeleton :
1 - 50 of 231 citations for DIRAS Family GTP Binding RAS Like 1 DIRAS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the RAS-GTP pull-down assay kit (Cytoskeleton #BK008) was used ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then incubated with 30 µg of Ras-GTP Binding Domain (RBD of human c-Raf kinase) fused with GST associated with beads (#RF02, Cytoskeleton) at 4°C for 1h ...
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bioRxiv - Cancer Biology 2023Quote: GTP-bound RAS levels were assessed with a RAS G-LISA Assay Kit per manufacturer’s instructions (Cytoskeleton INC., #BK131). 9 ξ 105 cells were plated in a 3D 24-well plate ...
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bioRxiv - Cancer Biology 2022Quote: ... a Ras GTP-ase immunosorbent assay (G-LISA) Activation Assay Kit (#BK131, Cytoskeleton) was used ...
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bioRxiv - Systems Biology 2020Quote: ... 300µg of total cell protein was used to pull-down GTP-bound RAS/RAF-RBD complexes according to the manufacturer’s instructions (Cytoskeleton). Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Equalized protein extractions were transferred to a Rho-GTP-binding protein pre-coated plate (Cytoskeleton, Inc.). The plate was placed on a microplate shaker at 300 rpm for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Pan-RAS (Cytoskeleton AESA02, 1:250), APOBEC3B (PA6889 ...
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bioRxiv - Cell Biology 2023Quote: ... resuspended in BRB80 with 1 mM GTP (Cytoskeleton), 1.25 μl BRB80 ...
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bioRxiv - Cancer Biology 2022Quote: RAS activity was analyzed with a Ras Activation Assay Biochem Kit (Cytoskeleton, BK008). The QGY cell lines containing the control group ...
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bioRxiv - Cell Biology 2021Quote: ... pH adjusted to 6.9 with KOH] supplemented with 1 mM GTP (Cytoskeleton BST06) and kept on ice ...
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bioRxiv - Molecular Biology 2020Quote: RhoA-GTP pulldown activation assays (Cytoskeleton, #BK036) were carried out according to protocol ...
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bioRxiv - Biophysics 2022Quote: ... GTP and Paclitaxel were purchased from Cytoskeleton, Inc ...
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bioRxiv - Biophysics 2020Quote: ... GTP-gamma-S was purchased from Cytoskeleton, Inc (#BS01) ...
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bioRxiv - Cancer Biology 2022Quote: RAS Activation Assay Biochem Kit™ (BK008; Cytoskeleton, Inc) was used to assess KRAS activity following manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: Guanosine Triphosphate (GTP) and paclitaxel (taxol) were purchased from Cytoskeleton Inc (Denver ...
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bioRxiv - Cancer Biology 2022Quote: ... the colorimetric-based RAS G-LISA activation assay (Cytoskeleton #BK131) was used ...
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bioRxiv - Biochemistry 2023Quote: ... 2.5 μl tubulin glycerol buffer and 0.75 μl 20 mM GTP (Cytoskeleton), the mixture was incubated at 37 ° C for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... of each was incubated for 15 min at 37°C in General Tubulin Buffer with 2.25 mg/mL of 99% pure porcine tubulin and 1 mM GTP (all from Cytoskeleton Inc.), in a final volume of 50 μL ...
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bioRxiv - Cell Biology 2024Quote: Pulldown of GTP-RhoA was performed according to the manufacturer’s instruction (Cytoskeleton; catalog #BK030). In brief ...
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bioRxiv - Cancer Biology 2022Quote: RAS activation was assessed by ELISA using a commercially available kit (Cytoskeleton: BK131) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Actin binding and polymerization were assessed using the actin binding protein kit (Cytoskeleton, BK013). Actin pellet gel bands were quantified by densitometry using FIJI.
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bioRxiv - Cancer Biology 2022Quote: We performed Rac1–GTP pull-down assay using the Rac1 activation kit (# BK035-S, Cytoskeleton) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: For GTP-RhoA pull down assay the Rho Activation Assay biochem kit (Cytoskeleton inc, Cat# BK036) was used as described in the product manual ...
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bioRxiv - Cancer Biology 2023Quote: ... The following steps were performed using the Cytoskeleton Ras-activation assay biochem kit (Cytoskeleton, #BK008) according to the manufacturer’s instructions ...
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Mapping chromatin state and transcriptional response in CIC-DUX4 undifferentiated round cell sarcomabioRxiv - Cancer Biology 2023Quote: The RAS GST-RBD activation kit was obtained from Cytoskeleton (Denver, CO, USA; cat #BK008). The protocol was performed following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The microtubules binding experiments were performed with the Microtubule Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton, Inc.) following manufacturer protocol.
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bioRxiv - Cancer Biology 2021Quote: ... GTP-pulldown and western blot procedures were performed according to RHOA/RAC1/CDC42 Activation Assay Combo kit (Cytoskeleton) manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: The levels of active GTP-bound Rac1 were measured by using Rac1 G-LISA kit (Cytoskeleton Inc., U.S.A) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... GTP-bound RhoA and Rac1 were immunoprecipitated from cells according to manufacturers’ protocols (BK-036, BK-128, Cytoskeleton).
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bioRxiv - Cell Biology 2024Quote: ... Rho-GTP was measured using the RhoA G-LISA Activation Assay Kit (Colorimetric Format; Cat# BK124) from Cytoskeleton, according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... In vitro microtubule-binding assay was performed using the microtubule-binding protein spin down assay kit (Cytoskeleton, Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... GTP-bound form of CDC42 was pulled down for analysis using the Cdc42 Pull-Down Activation Assay Kit (Cytoskeleton). FLAG-tagged protein pull-down assays were performed using DDDDK tagged protein purification kit (MBL) ...
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bioRxiv - Cell Biology 2023Quote: ... GTP-bound ARF6 was detected in cell lysates using the ARF6 Activation Assay Biochem Kit™ (Cytoskeleton, BK-033) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: For the determination of active GTP bound form of Cdc42 we used the G-LISA kit for Cdc42 from Cytoskeleton according to the protocol of the manufacturer.
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bioRxiv - Biophysics 2020Quote: ... Rac1-GTP and Cdc42-GTP using the RhoA and Cdc42 pull-down activation assay biochem kit (PN:BK036-S and BK034-S, Cytoskeleton, Inc.) following the protocol provided by the manufacturer ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysate preparation and measurement of RHOA-GTP levels were carried out with the RHOA G-LISA activation assay kit (#BK124, Cytoskeleton) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Activation of ARF6 (ARF6-GTP) was quantitated using the Arf6 Pull-Down Activation Assay Biochem Kit from Cytoskeleton (BK033-S, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... GAP assays were performed as described previously (Faix et al., 1998) except that GTP hydrolysis was quantitated using CytoPhos reagent (Cytoskeleton Inc) to measure the amount of free phosphate ...
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bioRxiv - Neuroscience 2024Quote: Active GTP-bound GTPase levels in brain lysates were measured using G-LISA activation assay kits for Rac1 (Cytoskeleton, Inc., BK128) and RhoA (Cytoskeleton ...
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bioRxiv - Neuroscience 2023Quote: ... or with PAK-p21 binding domain (PBD) beads (20 µg; Cytoskeleton Inc.) at 4°C under constant rotation to bind active RhoA or Cdc42 and Rac1 ...
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bioRxiv - Biochemistry 2024Quote: ... we used a MT binding protein spin-down assay kit (# BK029, Cytoskeleton, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... MT assays were performed using the MT Binding Protein Spin-down kit from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2022Quote: The Microtubule Binding Protein Spin-down assay was performed according to the manufacturers’ protocol (Cytoskeleton). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Cleared lysates were incubated with Rhotekin-Rho binding domain (RBD) beads (60 µg; Cytoskeleton Inc.) or with PAK-p21 binding domain (PBD ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were incubated in primary antibody diluted in TBST (1:500 anti-RHOA antibody, Cytoskeleton ARH04) or diluted in block (1:30,000 anti-a-alpha tubulin antibody ...
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bioRxiv - Molecular Biology 2019Quote: Co-pelleting assay was performed according to the F-actin binding co-sedimentation assay from Cytoskeleton Inc ...
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bioRxiv - Neuroscience 2023Quote: Actin bundling assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton Inc.) according to protocol of the manufacturer.
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bioRxiv - Neuroscience 2021Quote: ... incubated with a polyclonal antibody against actin (1:500; Cytoskeleton Inc.) and developed by chemiluminescence ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Cdc42 monoclonal antibody (1:250, Cytoskeleton, Cat. # ACD03), anti-Rac1 (1:250 ...
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bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...