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Citations for Cytoskeleton :
1 - 50 of 174 citations for Cow NADH ubiquinone oxidoreductase chain 5 MT ND5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... MT assays were performed using the MT Binding Protein Spin-down kit from Cytoskeleton, Inc ...
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bioRxiv - Biophysics 2019Quote: MTs were prepared by polymerizing 5 mg/ml tubulin (Cytoskeleton, Denver, CO) in BRB80 buffer (80 mM PIPES ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: MTs were polymerized by incubating 5 µM tubulin (4% HyLite 647 tubulin (Cytoskeleton #TL670M), ∼1% biotin tubulin (Cytoskeleton #T333P) ...
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bioRxiv - Cell Biology 2023Quote: ... rhodamine-labeled MTs were polymerized using a mixture containing 5% rhodamine-labeled tubulin (Cytoskeleton, TL590M), 5% biotin-labeled tubulin (Cytoskeleton ...
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bioRxiv - Biochemistry 2024Quote: ... we used a MT binding protein spin-down assay kit (# BK029, Cytoskeleton, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: MTs were polymerized (tubulin, Cytoskeleton Inc) in BRB80 buffer (80 mM Pipes/KOH pH 6.8 ...
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bioRxiv - Cell Biology 2020Quote: Commercial ELISA kits were purchased from Cytoskeleton (G-LISA) to quantify RhoA and Rac1 activation (Cytoskeleton) ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: MT polymerization assays were performed using a fluorescence-based Tubulin Polymerization kit (Cytoskeleton Inc, Denver CO, Cat. # BK011P) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... MT affinity/dead-head spin was performed by binding motor protein to newly polymerized MTs (unlabeled, 2uM Paclitaxel (Taxol (Cytoskeleton, Denver, CO, US, TXD01) -stabilized ...
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bioRxiv - Cell Biology 2022Quote: ... MT was polymerized by mixing unlabeled bovine brain tubulin (Cytoskeleton) and rhodamine-tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2019Quote: ... MTs were made by incubating 77% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Biochemistry 2023Quote: Microtubules (MTs) were prepared from porcine brain tubulin (Cytoskeleton, Inc. CO). Tubulin lyophilized pellets were resuspended in BRB80 (80 mM K-PIPES ...
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bioRxiv - Cancer Biology 2022Quote: RAS activation was assessed by ELISA using a commercially available kit (Cytoskeleton: BK131) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... GMPCPP MT seeds were prepared by incubating 77% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Biochemistry 2022Quote: ... paclitaxel-stabilised MTs containing 10% X-rhodamine-labelled and 10% biotin-labelled tubulin (Cytoskeleton) were polymerized as above and were left at room temperature for 48 hours before use in the TIRF assay.
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Cell Biology 2019Quote: ... GMPCPP MT bright seeds were prepared as described above by incubating 66.7% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Biophysics 2021Quote: ... Labeled MTs were generated by mixing 4μL of 20 μM Cy5 (PurSolutions) or Rhodamine (Cytoskeleton, Denver, CO) labeled tubulin with 9 μL unlabeled MTs and transferred to a 37°C water bath for 20 min and stabilized by adding 10 μM paclitaxel (Cytoskeleton ...
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bioRxiv - Neuroscience 2021Quote: Effects of tau and nocodazole on MT polymerization were tested using a Tubulin Polymerization Assay (Cytoskeleton Inc., Denver, CO). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... First double cycled GMPCPP MT seeds were prepared by incubating tubulin mix containing 70% unlabeled porcine brain tubulin (Cytoskeleton), 18 % biotin-tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... Seeds were formed by shearing larger MTs (GMPCPP-stabilized, labeled 1:40 with HiLyte 488 tubulin (Cytoskeleton, TL488M-B) with a Hamilton syringe (Avanti Polar Lipids ...
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bioRxiv - Neuroscience 2020Quote: ... and RAC1 were performed through the use of an ELISA-based Rho-GTPase activation assay kit (Cytoskeleton, Inc, catalog# BK124, BK127, BK128). Briefly ...
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bioRxiv - Biophysics 2021Quote: ... labeled tubulin with 9 μL unlabeled MTs and transferred to a 37°C water bath for 20 min and stabilized by adding 10 μM paclitaxel (Cytoskeleton, Denver, CO). MTs were stored at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mL DMSO (Cytoskeleton)] was added to the stock microtubule solution at room temperature.
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bioRxiv - Biophysics 2022Quote: ... and 5 μM tubulin-AlexaFluor647 (Cytoskeleton) were combined with 1mM DTT and 0.6mM GMP-CPP in M2B buffer and incubated spun at 300,000 g to remove aggregates ...
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bioRxiv - Cell Biology 2023Quote: ... 5% biotin-labeled tubulin (Cytoskeleton, T333P), and 90% unlabeled tubulin (Cytoskeleton ...
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bioRxiv - Microbiology 2023Quote: ... 5 µg of purified porcine tubulin (Cytoskeleton) was present in protein mixtures prior to Flag Ab immunoprecipitation.
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bioRxiv - Microbiology 2023Quote: Microtubules with ∼5% biotin–tubulin (Cytoskeleton inc.) and ∼5% Alexa488-labeled or Alexa647-labeled fluorescent tubulin (Cytoskeleton inc. ...
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bioRxiv - Neuroscience 2020Quote: ... Non-muscle actin (5 µM, APHL99, Cytoskeleton Inc.) was included in the pipette to prevent wash-out of plasticity63 ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μM β-actin (human platelets; Cytoskeleton) whose pH was adjusted to 7.25 with KOH ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μg/mL RhoA Activator II (Cytoskeleton, Inc.) or 2 μg/mL Rho A Inhibitor I (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μl of soluble TRITC-labelled tubulin (Cytoskeleton, TL590M) in phase separation buffer containing 500 ng ml−1 nocodazole was added for a final tubulin concentration of 5 μM ...
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bioRxiv - Biophysics 2023Quote: 5 μM porcine brain tubulin (Cytoskeleton, Cat No. T240) and 5 μM MAP7 MTBD were mixed with 2 mM GTP (final concentration ...
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bioRxiv - Biophysics 2021Quote: ... 5 μM F-actin (mixture 20% Biotinylated actin (Cytoskeleton Inc.)) was added to the mixture and incubated at RT for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... 22 μl of 5 mg ml−1 tubulin protein (Cytoskeleton, T240) was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Microbiology 2023Quote: ... and ∼5% Alexa488-labeled or Alexa647-labeled fluorescent tubulin (Cytoskeleton inc.) were prepared as described64 and stabilized with final 20µM taxol (Cytoskeleton inc.) ...
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bioRxiv - Biophysics 2020Quote: ... 5 μL of 10x polymerization buffer solution (BSA02; Cytoskeleton, Inc., Denver, CO) mixed with 45 μL of the previously produced solution were left for 1h at room temperature to polymerize ...
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bioRxiv - Cell Biology 2022Quote: ... 0.4 µl of 5 mg/ml biotin labeled tubulin (Cytoskeleton, #T333P-A), 20% glycerol ...
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bioRxiv - Cell Biology 2022Quote: ... in a ratio of 1:5 and then stabilizing with paclitaxel (Cytoskeleton). MTs and test proteins were incubated in binding buffer A (80 mM Pipes ...
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bioRxiv - Biochemistry 2023Quote: ... cells were pre-extracted for 5 minutes with cold CSK buffer (‘Cytoskeleton buffer’ ...
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bioRxiv - Immunology 2021Quote: ... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Cell Biology 2021Quote: Actin Polymerization Biochem Kit (Cytoskeleton) was used to investigate F-actin depolymerization by cofilin in the absence or presence of control protein BSA ...
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bioRxiv - Biophysics 2022Quote: ... provided along with the kit (Cytoskeleton) was used to detect G- and F-actins ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60min-old Tau:RNA or Tau:RNA:PEG coacervates were mixed with 5 μM porcine tubulin-Alexa594 (Cytoskeleton), and samples were pipetted onto the glass surface of an imaging dish (MaTek ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2023Quote: ... which was 5% fluorescently labelled with HiLyte 488 or HiLyte 647 conjugated porcine brain tubulin (Cytoskeleton), was assembled at 40 μM at 1 mM GTP for 30 mins at 37°C ...
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bioRxiv - Cell Biology 2020Quote: A solution of 5 μg/mL Fibronectin labeled with HiLyte fluor 488 (FNR02-A, Cytoskeleton, Inc. USA) in PBS was incubated on glass bottom petri dish for 30 min at 4°C and rinsed with cold PBS thrice ...
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bioRxiv - Cancer Biology 2021Quote: Rac1 and RhoA GTPase actitation was measured by G-LISA Rac1 activation assay Kit and G-LISA RhoA activation Kit (Cytoskeleton), according to manufacturer’s protocol ...