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1 - 12 of 12 citations for 9 TRIMETHYLSILYL FLUORENE 99% since 2019

• The mechanism of selective kinesin inhibition by kinesin binding protein.

  Joseph Atherton, et al., bioRxiv - Biochemistry 2020

  Quote: ... porcine tubulin (>99% pure, Cytoskeleton Inc.) was polymerised in MES polymerisation buffer (100 mM MES ...
• De Novo Variants in MRTFB have gain of function activity in Drosophila and are associated with a novel neurodevelopmental phenotype with dysmorphic features

  Jonathan C. Andrews, et al., bioRxiv - Genetics 2022

  Quote: Purified (>99%) human actin monomers (Cytoskeleton, APLC99) were prepared (5 mM Tris-HCl pH 8.0 ...
• Multi-monoubiquitination controls VASP-mediated actin dynamics

  Laura E. McCormick, et al., bioRxiv - Cell Biology 2023

  Quote: ... Lyophilized rabbit skeletal muscle actin (99% pure, Cytoskeleton) was resuspended in water and diluted to 10 mg/mL in fresh Ca-G buffer (2mM Tris ...
• Photoswitchable microtubule inhibitors enabling robust, GFP-orthogonal optical control over the tubulin cytoskeleton

  Li Gao, et al., bioRxiv - Cell Biology 2019

  Quote: 99% purity tubulin from porcine brain was obtained from Cytoskeleton Inc ...
• Hydrodynamic and Polyelectrolyte Properties of Actin Filaments: Theory and Experiments

  Ernesto Alva, et al., bioRxiv - Neuroscience 2022

  Quote: Actin from rabbit skeletal muscle (> 99% pure) was purchased from Cytoskeleton Inc ...
• Septin 2/6/7 complexes tune microtubule plus end growth and EB1 binding in a concentration- and filament-dependent manner

  Konstantinos Nakos, et al., bioRxiv - Cell Biology 2019

  Quote: ... which contained 9% HiLyte-488-tubulin (TL488M; Cytoskeleton Inc.) in BRB80 supplemented with 1 mM GTP ...
• PFN2 and NAA80 cooperate to efficiently acetylate the N-terminus of actin

  Rasmus Ree, et al., bioRxiv - Biochemistry 2020

  Quote: ... For measurements including actin (human platelet actin, 99% pure, from Cytoskeleton, Inc., catalog nr: APHL99), the buffer was 5 mM Tris ...
• In vitro reconstitution of branched microtubule nucleation

  Ammarah Tariq, et al., bioRxiv - Cell Biology 2019

  Quote: ... of each was incubated for 15 min at 37°C in General Tubulin Buffer with 2.25 mg/mL of 99% pure porcine tubulin and 1 mM GTP (all from Cytoskeleton Inc.), in a final volume of 50 μL ...
• Extraction of accurate cytoskeletal actin velocity distributions from noisy measurements

  Cayla M. Miller, et al., bioRxiv - Biophysics 2021

  Quote: ... After a 9 minute incubation with 50 nM SiR-Actin (Cytoskeleton Inc., CY-SC001), the sam-ple was incubated with Prolong Live Antifade Reagent (In-vitrogen P36975 ...
• Molecular mechanism of a parasite kinesin motor and implications for its inhibition

  Alexander D. Cook, et al., bioRxiv - Biophysics 2021

  Quote: ... or biotin labelled Sus scrofa brain tubulin - except X-rhodamine which was from Bos taurus -(catalogue numbers T240C, TL620M T333P, respectively, all >99% pure, Cytoskeleton Inc.) was reconstituted to 10 mg/mL in BRB80 (80 mM PIPES pH 6.8 ...
• Nanoscale organization of Actin Filaments in the Red Blood Cell Membrane Skeleton

  Roberta B. Nowak, et al., bioRxiv - Biophysics 2021

  Quote: ... Washed cells were diluted 1:9 and stained with 50 nM SiR-actin (Spirochrome/Cytoskeleton) for 30 minutes (Lukinavicius et al. ...
• ERK3/MAPK6 dictates Cdc42/Rac1 activity and ARP2/3-dependent actin polymerization

  Katarzyna Bogucka-Janczi, et al., bioRxiv - Cell Biology 2022

  Quote: Guanine nucleotide exchange (GEF) activity of ERK3 (9-327 aa) was further evaluated using RhoGEF Exchange Assay (Cat# BK100, Cytoskeleton). Assay was performed according to the manufactures’s instructions and included Dbs GEF as a positive control.