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Citations for Cytoskeleton :
1 - 44 of 44 citations for 4 Methoxyestradiol Unlabeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... 75 μM unlabeled tubulin (Cytoskeleton) and 5 μM tubulin-AlexaFluor647 (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μM unlabeled porcine tubulin (Cytoskeleton) prepared in binding buffer containing 10 mM DTT and 0.2 mM PMSF was added and incubated overnight on a shaker in a cold room ...
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bioRxiv - Cell Biology 2023Quote: ... and 90% unlabeled tubulin (Cytoskeleton, T240) in BRB80 buffer (80 mM PIPES ...
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bioRxiv - Cell Biology 2021Quote: ... and unlabeled G-actin (AKL99, Cytoskeleton Inc) successively into the assembly buffer and let them equilibrate at 37 °C for 1 hour ...
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bioRxiv - Biophysics 2021Quote: ... 20 µM unlabeled porcine brain tubulin (Cytoskeleton #T240) was polymerized at 37°C for 2 hours in BRB80 (80mM PIPES pH 6.8 ...
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bioRxiv - Cell Biology 2021Quote: Microtubules were prepared using unlabeled porcine brain tubulin (Cytoskeleton) reconstituted in ice-cold BRB80 buffer to 5 mg/ml and polymerized in the presence of 1mM Mg-GTP for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 2 µL of 10 mg/mL unlabeled tubulin (Cytoskeleton) was mixed with 2 µL o f 1 mg/mL biotin-tubulin and 2 µL of 1 mg/mL dye-labeled tubulin on ice ...
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bioRxiv - Biochemistry 2023Quote: ... Taxol-stabilized microtubules assembled from unlabeled porcine tubulin (Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Cell Biology 2022Quote: ... MT was polymerized by mixing unlabeled bovine brain tubulin (Cytoskeleton) and rhodamine-tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2019Quote: ... MTs were made by incubating 77% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Biophysics 2019Quote: ... by incubating the tubulin mix containing 70% unlabeled porcine brain tubulin (Cytoskeleton), 18% biotin-tubulin (Cytoskeleton ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 μL of 10 mg/mL unlabeled tubulin (Cytoskeleton Inc., #T240) was mixed on ice ...
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bioRxiv - Biophysics 2023Quote: A mixture of unlabeled porcine brain tubulin (Cytoskeleton, Inc. Cat. No. T240) was copolymerized with either rhodamine-labelled tubulin (Cytoskeleton ...
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bioRxiv - Microbiology 2021Quote: Rabbit skeletal muscle actin was purchased (unlabeled and pyrene-labeled actin; Cytoskeleton Inc.). All actin was maintained in G buffer (5 mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... GMPCPP MT seeds were prepared by incubating 77% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Cell Biology 2022Quote: Microtubules were polymerized (purified porcine tubulin unlabeled and HiLyte-647-labeled, Cytoskeleton Inc) in BRB80 buffer [80 mM Pipes/KOH pH 6.8 ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μM rabbit skeletal muscle actin (90% unlabeled and 10% rhodomine-actin) (Cytoskeleton Inc.), 1 μM Shi were incubated in 6.5 mM HEPES ...
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Biophysics 2019Quote: Unlabeled and labeled (biotin and TRITC) lyophilized tubulin from porcine brain were purchased from Cytoskeleton Inc (Denver ...
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Cell Biology 2019Quote: ... Actin filaments (28 μM) were made by incubating 83% unlabeled G-actin (APHL99; Cytoskeleton Inc.) with 8.5% biotin-G-actin (AB07 ...
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bioRxiv - Cell Biology 2021Quote: Microtubules were polymerized (unlabeled and HiLyte-647-labeled porcine brain tubulin, Cytoskeleton Inc #T240 and #TL670M) in BRB80 buffer (80 mM Pipes/KOH pH 6.8 ...
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bioRxiv - Cell Biology 2021Quote: ... Different types of labelled and unlabeled purified tubulin used in the assays were either purchased from Cytoskeleton or purified as described previously (57 ...
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bioRxiv - Cell Biology 2019Quote: ... GMPCPP MT bright seeds were prepared as described above by incubating 66.7% unlabeled tubulin (T240; Cytoskeleton Inc.) with 11.5% biotin-tubulin (T333P ...
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bioRxiv - Cell Biology 2019Quote: ... First double cycled GMPCPP MT seeds were prepared by incubating tubulin mix containing 70% unlabeled porcine brain tubulin (Cytoskeleton), 18 % biotin-tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: GMPCPP-stabilized microtubules were assembled using 50 μM unlabeled porcine tubulin containing 1:12 biotin-labeled porcine tubulin (Cytoskeleton) and polymerized in the presence of 1mM GMPCPP (Jena Bioscience ...
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bioRxiv - Biophysics 2020Quote: ... 10% rhodamine-labeled F-actin was prepared by co-polymerizing 0.9 μM unlabeled actin monomers and 0.1 μM rhodamine-labeled actin monomers (Cytoskeleton, AR05) in the presence of G-Mg (2 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: Porcine Tubulin at 1:5 ratio (rhodamine-labeled: unlabeled) (cat. no. T240 and TL590M-A respectively, Cytoskeleton, Inc., Denver, USA) was reconstituted to 4.0 mg/mL in cold polymerization buffer ...
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bioRxiv - Cell Biology 2021Quote: ... MT affinity/dead-head spin was performed by binding motor protein to newly polymerized MTs (unlabeled, 2uM Paclitaxel (Taxol (Cytoskeleton, Denver, CO, US, TXD01) -stabilized ...
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bioRxiv - Biophysics 2023Quote: ... and ∼4% rhodamine-tubulin (Cytoskeleton) were incubated with 1 mM GMPCPP or 1 mM GTP with 10 mM taxol at 37°C for 3 h ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of biotinylated tubulin (Cytoskeleton) and 100 μg of unlabelled pig tubulin in BRB80 buffer (80 mM PIPES pH 6.8 ...
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bioRxiv - Physiology 2021Quote: ... podocytes were fixed in 4% paraformaldehyde and 4% sucrose and stained with Acti-stain™ 488 Phalloidin (Cytoskeleton) and 50 cells per condition were assessed ...
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bioRxiv - Biophysics 2023Quote: ... or α4A/β2A tubulins ∼4% biotin-tubulin (Cytoskeleton) and ∼4% rhodamine-tubulin (Cytoskeleton ...
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bioRxiv - Molecular Biology 2023Quote: ... SiR-Tubulin incubation was performed for 4 h (Cytoskeleton, with 2 μM SiR-Tubulin and 10 μM verapamil) ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed in PFA (4% PFA in Cytoskeleton Buffer (CB) – in mM ...
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bioRxiv - Microbiology 2024Quote: Microtubules were made by mixing 4 μg of HiLyte Fluor 488 tubulin (Cytoskeleton), 4 μg of biotinylated tubulin (Cytoskeleton ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: MTs were polymerized by incubating 5 µM tubulin (4% HyLite 647 tubulin (Cytoskeleton #TL670M), ∼1% biotin tubulin (Cytoskeleton #T333P) ...
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bioRxiv - Cell Biology 2020Quote: In order to constitutively activate RhoA vessels at day 4 were perfused with Rho Activator II (Cytoskeleton) at a concentration of 2 µg/mL for four hours prior to use in permeability tests or fixation for immunocytochemistry ...
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bioRxiv - Microbiology 2020Quote: ... Samples were fixed in 4% (v/v) Paraformaldehyde Aqueous Solution (15710-s, EM Grade) in PEM (Cytoskeleton-preserving buffer PIPES-EGTA-Magnesium ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... actomyosin contractility of MECs stably expressing an inducible ROCK construct was induced overnight using 4-hydroxytamoxifen and the actin cortex of these cells was labelled with SiR-actin dye (Cytoskeleton) according to manufacturer’s instructions prior to laser ablation.
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...