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Citations for Cytoskeleton :
251 - 300 of 334 citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: The F-actin/G-actin ratio was measured using G-Actin/F-Actin In Vivo Assay Biochem Kit (#BK037, Cytoskeleton, Inc.), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: F- and G-actin fractions from lysates were prepared using a G-Actin/F-Actin in Vivo Assay Biochem kit (Cytoskeleton, #BK037) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Western blots to determine the ratio of G-actin to F-actin were performed on retinal lysates that were processed using a G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... NIH3T3 cells were imaged using four wavelengths (405 nm for tagBFP; 488 nm for Citrine; 561 nm for mCherry or FusionRed; 674 nm for SiR700-Tubulin Kit, #CY-SC014, Cytoskeleton Inc.).
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bioRxiv - Genetics 2024Quote: Mouse podocytes were lysed and examined for their total G-actin and F-actin levels using a G-actin/F-actin In vivo Biochemical Assay Kit (Cytoskeleton, CO). Briefly ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... labeled at a 1:40 ratio with HiLyte Fluor 647 tubulin (Cytoskeleton, Denver, CO), were flowed into the chamber and immobilized by interaction with rigor kinesin-1E236A ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... Actin-F was labelled with Sir-Actin dye (1:5000, Cytoskeleton, Denver, CO, USA) for 4 h prior to infection.
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bioRxiv - Cell Biology 2023Quote: ... labeled at a 1:40 ratio with HiLyte Fluor 488 tubulin (Cytoskeleton, Denver, CO), were flowed into the chamber and immobilized by interaction with rigor kinesin-1E236A.
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Neuroscience 2023Quote: ... F-actin staining was done using Acti-stain 555 phalloidin (1:2000, Cytoskeleton, PHDH1A) and counter stained with DAPI.
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bioRxiv - Biophysics 2024Quote: ... cell nuclei were labeled by addition of SiR-Hoechst [45] (1 μM; Cytoskeleton Inc.) to Cnt-Pr-D+1.2 mM Ca2+ bath media for 1 hour prior to imaging ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 30 min with 0.5 μM of SiR-actin or 60 min with 0.5 μM of SiR-tubulin and 10 μM of verapamil in culture medium (SiR Cytoskeleton Kit, Spirochrome SC006).
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bioRxiv - Neuroscience 2020Quote: ... and RAC1 were performed through the use of an ELISA-based Rho-GTPase activation assay kit (Cytoskeleton, Inc, catalog# BK124, BK127, BK128). Briefly ...
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bioRxiv - Neuroscience 2020Quote: RhoA activation was assessed with the G-LISA activation kit in accordance with the manufacturer’s instructions (Cytoskeleton Inc., catalog no. BK124-S). Active GTP-bound RhoA was detected with HRP-conjugated anti-RhoA antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... The RhoActivity assay was performed using the RhoA pull-down activation assay biochem kit (Bead pull-down format) (Cytoskeleton, Cat No. BK036). The assay was carried out by incubating 800 µg of total cell lysate with 50 µg of Rhotekin as described in the manufacturers protocol following which western blots were performed ...
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bioRxiv - Cell Biology 2023Quote: Measurements of the microtubule-stimulated ATPase activities of KIF1A MD-NC-CC1-FHA and various mutants were performed by using the HTS Kinesin ATPase Endpoint Assay Biochem Kit (Cytoskeleton, Inc., BK053). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein was extracted from multiple cell cultures (G411, G729, G797) using the RAC1 Activation Assay Biochem Kit per manufacturer’s instructions (Cytoskeleton Inc, #BK035-S) five days post transduction with lentiviral non-targeting or GJB2 shRNA ...
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bioRxiv - Neuroscience 2024Quote: ... and a 642 nm laser was used for JF635 or JF646 HaloTag-ligands and SirActin (SiR-Actin Kit, Cytoskeleton Inc, CY-SC001). Fluorescence emission was detected by pco.edge CLHS sCMOS cameras with appropriate bandpass filters LBF 405/488/561/642 + SBS LP 560 nm ...
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Biophysics 2021Quote: ... Washed cells were diluted 1:9 and stained with 50 nM SiR-actin (Spirochrome/Cytoskeleton) for 30 minutes (Lukinavicius et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Developmental Biology 2024Quote: ... Cysts were incubated in a culture medium containing SPY650-actin (Cytoskeleton, Inc., 1:2000 dilution) for 3 h before indentation ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used included anti-β-actin (1:1,000, AAN01, Cytoskeleton, Inc., Denver, CO, USA), anti-AMPKα (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with a 1:1000 dilution of SPY555-Actin Probe (CY-SC202, Cytoskeleton) in complete DMEM at 37°C for three hours prior to imaging ...
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bioRxiv - Cell Biology 2023Quote: ... Phalloidin (Alexa Fluor 488 and 670, Cytoskeleton Jomar, PHDG1-A, PHDN1-A, ICC, 1:500).
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bioRxiv - Biophysics 2023Quote: ... F-actin was labeled with rhodamine-phalloidin (PHDR1; Cytoskeleton, Inc., Denver, CO, USA; 1:167).
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bioRxiv - Bioengineering 2024Quote: ... with cell morphology and nucleus visualized using Acti-stain 555 phalloidin (1:2000, Cytoskeleton, PHDH1A) and DAPI (2ng/ml ...
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bioRxiv - Cancer Biology 2019Quote: The G-actin/F-actin assay was performed with a G-actin/F-actin in vivo assay kit (Cytoskeleton, Inc., Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: The G-actin/F-actin ratio in 229 R cells was assayed using the G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Denver, CO, USA). 229 R cells were scraped in LAS2 buffer and lysed ...
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bioRxiv - Cell Biology 2019Quote: Soluble G-actin was separated from filamentous F-actin using the In Vivo G-actin/F-actin Assay Kit (Cat# BK037, Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... cells were rinsed with warmed PBS and cell lysates prepared in cell lysis buffer as instructed and provided by the RhoA or Rac1 pull-down instruction manual and kit (Cytoskeleton, Inc. Denver, CO). Protein quantitation was performed by BCA method and 600 μg of protein was incubated with 50 μg Rhotekin-RBD beads for RhoA pull-down or with 10 µg of PAK-PBD beads for Rac1 pull-down at 4°C for 1 h ...
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bioRxiv - Neuroscience 2024Quote: F-actin and G-actin content was measured using the G-actin/F-actin in vivo assay biochemical kit (Cytoskeleton Inc., Denver, CO) as previously described in (Laguesse et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and then placed in 1.5 ml imaging media containing 1 μM SiR-DNA (CY-SC007, Cytoskeleton) + 10 μM Verapamil (for nucleus staining ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μm carboxylate beads (01-02-103, micromer) were coated with biotinylated laminin (LMN03, Cytoskeleton, Inc.) or FN7-10 (a four-domain segment of fibronectin responsible for cell binding and containing the RGD and PHSRN motifs102 ...
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bioRxiv - Biochemistry 2020Quote: ... Fixed tissues were washed in PBS (and stained with SiR-actin (1:1000 dilution, Cytoskeleton Inc.) for 3 hours at room temperature (RT ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained with 1 µM SiR-actin and 10 µM verapamil (Cytoskeleton, Inc., CY-SC001) for 1-3 hours before imaging ...
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bioRxiv - Microbiology 2020Quote: ... The cells were washed twice with PBS and incubated with rhodamine phalloidin (1:500) (Cytoskeleton Inc.) in IF washing buffer for 30 minutes at room temperature in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with monoclonal mouse anti-human RhoA primary antibody (# GL01A; 1:250; Cytoskeleton Inc.), followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02 ...
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bioRxiv - Cell Biology 2019Quote: ... followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02; 1:62.5; Cytoskeleton Inc.) on a shaker at 300 rpm at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... 0.025% saponin and 1 mM ATP were added followed by 0.45 μM rhodamine actin (Cytoskeleton Inc). The culture medium was carefully removed by pipette and enough permeabilization buffer was added to cover cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were incubated in primary antibody diluted in TBST (1:500 anti-RHOA antibody, Cytoskeleton ARH04) or diluted in block (1:30,000 anti-a-alpha tubulin antibody ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were made by mixing 1 μl of HiLyte Fluor 488 tubulin (2 mg/ml, Cytoskeleton), 2 μl biotinylated tubulin (2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed by western blot using anti-ubiquitin antibody (P4D1, Cytoskeleton AUB01-HRP, 1:1000) and visualized with Clarity Western ECL Substrate (BioRad).
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... actin filaments (Acti-stain 670 phalloidin, 1:600 dilution; Cat: SKU PHDN1, Cytoskeleton, Denver, CO USA). Imaging was performed using a spinning disk confocal microscope (Yokogawa CSU-X1 ...
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bioRxiv - Biophysics 2020Quote: ... F-actin filaments and the nuclei were labeled with 1 µM of SiR-actin (Cytoskeleton, Denver, USA) and 1 µg/ml of Hoechst 33342 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoblots were performed using the following primary antibodies: rabbit anti-KIF15 (1:1000; Cytoskeleton Inc., Cat#AKIN13), rabbit anti-KIF2A (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2 μl of 1 mg/ml HiLyte Fluor 647 or X-rhodamine-labelled tubulin (#TL670M, #TL6290M, Cytoskeleton) and 0.5 μl of 0.5 mg/ml biotin tubulin (#T333P ...
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bioRxiv - Biophysics 2019Quote: ... the primary antibodies used in the study were sheep anti-tubulin (2.5 μg mL−1, ATN02, Cytoskeleton) and mouse anti-TOM20 (2.5 μg mL−1 ...
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bioRxiv - Cell Biology 2023Quote: Stock actin (10 mg/ml) was diluted 1:10 into ATP-supplemented general actin buffer from Cytoskeleton Inc ...