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Citations for Corning :
1 - 50 of 2260 citations for Recombinant HIV 1 GP120 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... ZIKV envelope or HIV-1 proteins were coated overnight at 4°C on the wells of 384-well plates (Corning Life Sciences). Each protein was coated at 2 μg/mL in 15 μl in sodium bicarbonate ...
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bioRxiv - Immunology 2020Quote: ... Plant-expressed 6-His tagged ZE3 at 50 ng/well was bound to 96-well high-binding polystyrene plates (Corning Inc, Corning, NY, USA), and the plates were blocked with 5% nonfat dry milk in PBST ...
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bioRxiv - Immunology 2020Quote: ... Plant-expressed 6-His tagged ZE3 at 50 ng/well was bound to 96-well high-binding polystyrene plates (Corning Inc, Corning, NY, USA), and the plates were blocked with 5% nonfat dry milk in PBST ...
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bioRxiv - Cancer Biology 2019Quote: ... recombinant proteins were immobilized on a 96-well flat bottom plate (Corning, CLS3595) by incubation at 4 °C for 12-16 hr ...
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bioRxiv - Microbiology 2020Quote: HIV-1 specific binding antibody titers were performed by coating high-binding 384 well plates (Corning, Corning, NY) overnight at 4°C with 1086C and Con6 gp120s ...
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bioRxiv - Microbiology 2020Quote: HIV-1 specific binding antibody titers were performed by coating high-binding 384 well plates (Corning, Corning, NY) overnight at 4°C with 1086C and Con6 gp120s ...
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bioRxiv - Microbiology 2021Quote: ... HIV-1 infected donor T cells were separated from target T cells by a 0.4μm transwell insert (Corning). Experiments to quantify cell-to-cell versus cell-free infection in the presence and absence of a transwell were performed in equivalent volumes (600μl) ...
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bioRxiv - Biochemistry 2020Quote: Recombinant SARS-CoV-2 Spike protein was coated onto high-binding 96-well plates (Corning, 3690) at 2 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... His-S1 or ecto-spike proteins were plated on half-area high-binding 96-well polystyrene plates (Corning) at 1 μg/mL or 0.5 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% HI-FBS (Corning®), 1% (v/v ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... Recombinant SARS-CoV-2 S protein (10 ng/well) was coated onto a 96-well plate (Costar assay plate, Corning, 3369) for 24 h at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... human recombinant EGF (Corning, Bedford, MA), hydrocortisone (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... 4% heat-inactivated FBS (HI-FBS, FBS from Corning, #35-010-CV ...
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bioRxiv - Cancer Biology 2020Quote: ... recombinant protein diluted in PBS or SUIT2 conditioned media was immobilized overnight on high binding ELISA 96-well microplates (Corning, cat. no. 9018). The wells were then washed three times with 200 µL PBS with 0.05% Tween-20 (PBS-T ...
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bioRxiv - Microbiology 2021Quote: HEK 293T cells (NIH HIV Reagent Program) and Hela cells (ATCC, VA) were grown in Dulbecco’s-modified Eagles medium (Corning, NY) supplemented with 10% fetal clone III (Cytiva Life Sciences ...
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bioRxiv - Bioengineering 2020Quote: ... or recombinant human laminin 521 (10μg/mL rLaminin, Corning). Wells were uniformly coated using 125 μL/well (223 μL/cm2 ...
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bioRxiv - Biochemistry 2022Quote: ... OX40 overexpressing Jurkat cells engineered with an NFκB luciferase reporter were seeded at 80,000 cells/well in 20 µl RPMI containing 1% L-glutamine and 10% HI FBS in a 384-well tissue culture plate (Corning Inc., 3985BC). Anti-OX40 antibody formats were serially diluted three-fold in media starting at 30 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... the optimized reaction mixture (1.5 nM of 6His-MSN and 25 nM of FITC-CD44 peptide in the presence of anti-His-Tb at final 1:1000 dilution) was dispensed at 5 µL per well into 1536-well black plates (Corning Costar, #3724). 0.1 µL of library compound dissolved in DMSO was added using a pin tool integrated with the Biomek NX automated liquid handling workstation (Beckman Coulter) ...
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bioRxiv - Neuroscience 2023Quote: ... 10% heat inactivated fetal bovine serum (HI FBS) (USA Sourced, Corning, USA), 1x antibiotic/antimycotic (P/S ...
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bioRxiv - Microbiology 2022Quote: ... transfection mixes were prepared using PEI (4 μl per 1 μg of DNA) with the GFP-HIV (6 μg) and VSV-G (4 μg) constructs in serum-free DMEM medium (Corning #10-013-CM) and added dropwise to HEK293T cells that were seeded onto 10-cm dishes a day prior at a density of 5-6 × 106 cells in 10 ml of fresh DMEM with 10% heat inactivated FBS (Sigma-Aldrich #14009C) ...
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bioRxiv - Cancer Biology 2019Quote: ... 40ng/ml human recombinant basic fibroblast growth factor (bFGF, Corning), 1xB27 (Gibco) ...
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bioRxiv - Microbiology 2023Quote: Peripheral blood mononuclear cells (PBMCs) from HIV-seronegative donors (Vitalant) were isolated by Ficoll-Hypaque density gradient centrifugation (Corning, Inc., Cat. #25-072-Cl) at 2000 rpm (∼ 850 x g ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 20ng/ml human recombinant epidermal growth factor (EGF, Corning), 40ng/ml human recombinant basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2024Quote: ... were coated with recombinant CD45RO at 0.2µg/mL in PBS (Corning) overnight at 4C ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were frozen in 10% DMSO/10% heat inactivated (HI) human AB serum (Corning) for later flow cytometry staining ...
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bioRxiv - Molecular Biology 2020Quote: ... TASOR knockout cells expressing HA-BirA-tagged TASOR variants were grown in square 500 cm2 dishes (Corning) in DMEM ...
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bioRxiv - Neuroscience 2021Quote: ... on either human recombinant laminin LN521 (Biolamina #LN521-02) or Matrigel (Corning #356237) coated plates ...
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bioRxiv - Biochemistry 2023Quote: HEK-293 stably expressing the Glosensor cAMP reporter (Promega Corp.)21 and epitope-tagged NK1R were seeded in a black-walled 96-well plate flat clear bottom (Corning) and grown to confluency at 37°C in a 5% humidified CO2 incubator ...
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bioRxiv - Microbiology 2021Quote: ... was set up at 6-8 mg/mL in loading buffer containing 0 or 500 mM NaCl as 2 μL crystallization drops (1 μL protein: 1 μL reservoir solution) in 96-well plates (Corning) using commercial Classics II ...
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bioRxiv - Cancer Biology 2021Quote: ... c-Met and EGFR signalling were concomitantly stimulated with recombinant human EGF (Corning, NY, USA) at a final concentration of 20 nM ...
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bioRxiv - Immunology 2024Quote: Recombinant full-length HA ectodomains or heads were coated on 96-well plates (Corning, #9018) in PBS at 2 µg/ml (full-length ...
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bioRxiv - Genomics 2021Quote: ... for imaging or poly-D-lysine coated 100 mm culture plates for Hi-C experiments (Corning BioCoat 356469), and maintained at 4° C for a few hours or overnight until nuclei were settled and adhering to the dish ...
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bioRxiv - Biochemistry 2019Quote: ... as a methyl group donor and biotinylated oligonucleotides of varying sizes bound on avidin-coated high-binding Elisa plates (Corning) as described54 ...
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bioRxiv - Biochemistry 2023Quote: ... 21 and NK1R wide-type or epitope-tagged NK1R were seeded in a white-walled 96-well flat clear bottom plate (Corning #3610) and grown to confluency at 37°C in a 5% humidified CO2 incubator ...
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bioRxiv - Systems Biology 2019Quote: ... HT-1080 cells were cultured in DMEM Hi-glucose media (Corning Life Science, Cat. No. MT-10-013-CV) supplemented with 1% non-essential amino acids (NEAAs ...
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bioRxiv - Microbiology 2020Quote: Ten recombinant CD antigens were diluted to 0.5 mg/mL in Phosphate Buffered Saline (Corning Cellgro), or concentrated up to 0.5 mg/mL using 10K MWCO Amicon Ultra-15 centrifugal filters (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... the protein was coated to high protein-binding ELISA plates (Corning) at a concentration of 0.5 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: HT-1080 and 293T cells were cultured in DMEM Hi-glucose medium (Cat# MT-10-013-CV, Corning Life Science) supplemented with 1% non-essential amino acids (NEAAs ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (v/v) FBS-HI 24-72 h prior to infection using 150-mm tissue culture-treated plates (Corning) at 6×106 cells per dish (for mass-spectrometry (MS ...
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bioRxiv - Immunology 2022Quote: ... and Omicron (BA.1) spike protein by coating of high-binding 96-half-well plates (Corning 3690) with 50 μl per well of a 1μg/ml indicated protein solution in Phosphate-buffered Saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... 3HA-tagged F508del-CFTR expressing baby hamster kidney (BHK) cells were seeded in 96-well plates (Corning half area, black-sided, clear bottom) at 15,000 cells per well ...
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bioRxiv - Biochemistry 2023Quote: HEK293T stable cell lines expressing N-terminally tagged NanoLuc-chaperones (HSP70 (HSPA8) or HSP90 (HSP90AB1)) were maintained in 10-cm petri dishes (Corning, 08-772-22) grown in a humidity-controlled incubator at 37° C with 5% CO2 in DMEM (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... The SVF from interscapular brown fat pads of 6 – 8 mice were then resuspended in 6 ml DMEM supplemented with 10 % HI NCBS and plated on 3 wells of a six well plate (Corning, #3506). After 4 and 24 h ...
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bioRxiv - Bioengineering 2021Quote: 6x-His tag antibodies were coated at 2 ug/mL in PBS onto 96-well half-area high binding plates (Corning, 3690) overnight at 4°C or 2 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... We diluted cells to 3.3 x 106 cells/mL RPMI/10%HI-FBS and transferred 900 µL each to 2 mL external thread cryogenic vials (Corning, Corning, NY) for each experimental condition ...
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bioRxiv - Immunology 2022Quote: ... The eluted protein was further purified by SEC with Phosphate-Buffered Saline (PBS, 1×, Corning, 21-040-CM) as the mobile phase on the Superose 6 Increase 10/300 GL (Millipore Sigma ...
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bioRxiv - Immunology 2020Quote: ... 900 ng of purified dengue consensus envelope domain III tagged with 6D8 epitope (Kim et al., 2015) were bound to a 96-well high-binding polystyrene plate (Corning Inc, Corning, NY, USA). After a 1-hour incubation at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... We diluted cells to 3.3 x 106 cells/mL RPMI/10%HI-FBS and transferred 900 µL each to 2 mL external thread cryogenic vials (Corning, Corning, NY) for each experimental condition ...
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bioRxiv - Immunology 2024Quote: ... BMDMs were then subjected to serum shock by replacing serum-free media with RPMI supplemented with 50% (v/V) HI horse serum (Corning, CAT#35030CV) at 37⁰C in air enriched with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: Purified MN100 protein constructs at 1 μg/mL were coated in the wells of 96-well microtiter plates (Corning) in carbonate buffer (50 mM Na2CO3-NaHCO3 ...