Labshake search
Citations for Beckman :
1 - 50 of 64 citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
-
bioRxiv - Microbiology 2024Quote: ... The virus was enriched by ultracentrifugation (Beckman Coulter Optima™ L-80XP) at 246,347g for 4 hours at 4 °C ...
-
bioRxiv - Microbiology 2021Quote: ... each strain’s optical density (OD500nm) was measured by spectrophotometry (Beckman Coulter). Strains were then each washed and diluted to an OD of 1 via centrifugation and resuspension in 1X phosphate buffered saline (PBS) ...
-
bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
-
bioRxiv - Synthetic Biology 2020Quote: Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 28) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor) for 2h at 4 °C ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
-
bioRxiv - Biochemistry 2021Quote: ... The virus-containing filtrate was then further purified using an ultracentrifuge (Optima XPN-90 Ultracentrifuge, Beckman Coulter, USA) with a cycle of 1,000 rpm (116 g ...
-
bioRxiv - Microbiology 2020Quote: ... The virus was then purified over a 20% sucrose cushion by centrifugation at 35,000 rpm (Beckman Coulter SW41 rotor) for 3 h at 4°C ...
-
Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
-
bioRxiv - Molecular Biology 2020Quote: ... Virus particles in the supernatant were pelleted by ultracentrifugation at 45.000 rpm for 45 min (Beckman 70.1 Ti Rotor) over a pad of 15% sucrose in 10 mM Tris-HCl buffer (pH 7.4) ...
-
bioRxiv - Microbiology 2024Quote: ... Virus RNA was extracted with a magnetic bead-based RNA extraction kit (RNAdvance Viral Reagent kit, Beckman Coulter, C63510) on an epMotion® 5073t liquid handler (Eppendorf ...
-
bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
-
bioRxiv - Biophysics 2023Quote: ... Virus-containing supernatants were collected at 48 hours and 72 hours after transfection and concentrated using ultracentrifugation (Ultraclear, Beckman 344058). The transduction capacity of the viral vector product was confirmed by culturing non-transfected HEK293T cells with the virus after 24 hours and assessing via fluorescent imaging.
-
bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and virus pelleted through 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
-
bioRxiv - Cell Biology 2021Quote: Fluorescence intensity of different strains stably expressing Ste3-GFP-DUb was measured using a CytoFLEX flow cytometer (Beckman Coulter) with 488 nm laser excitation ...
-
bioRxiv - Microbiology 2019Quote: ... cholerae strain BGT49 was extracted using the PacBio Phenol-Chloroform recommended protocol and cleaned using AMPure XP beads (Beckman Coulter). Purified DNA was sent to the University of Washington PacBio Sequencing Services ...
-
bioRxiv - Genetics 2021Quote: ... The sheared DNA fragments were then purified for each of the 16 mice strains using AMPure XP Beads (Beckman, USA), following the instructions of the reagent supplier.
-
bioRxiv - Microbiology 2023Quote: ... After ON growth at 37° C with shaking strain frequencies were measured using flow cytometry (Beckman Coulter CytoFLEX flow cytometer). Relative fitness was calculated as the ratio of relative frequency of the strains at the end of the experiment to their initial frequency.
-
bioRxiv - Evolutionary Biology 2022Quote: Competition assays were performed by using GFP-tagged strains to distinguish between populations with flow cytometry (CytoFLEX Platform Beckman Coulter Life Sciences, USA). Parameters were ...
-
bioRxiv - Immunology 2020Quote: ... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
-
bioRxiv - Molecular Biology 2023Quote: Proteins were collected by centrifugation (Beckman-Coulter JA-14 rotor ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein aggregates were pelleted by ultracentrifugation of the proteins individually at 80,000 rpm in TLA 100 (Beckman Coulter) for 15 minutes ...
-
bioRxiv - Immunology 2021Quote: ... recombinant protein and anti-CD14 antibody (Beckman) were used at 20 μg/ml ...
-
bioRxiv - Microbiology 2020Quote: ... The proteins were sedimented in the SW40Ti rotor (Beckman Coulter) at 100,000 × g for 17 h at 4 °C ...
-
bioRxiv - Microbiology 2022Quote: Supernatant containing proteins and membrane were ultracentrifuged at 200,000xg (Beckman Coulter Ultracentrifuge ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins and liposomes were mixed in polycarbonate tubes (Beckman Coulter) and incubated for 30 min at RT ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein complexes were then fractionated by centrifugation in an SW 41Ti rotor (Beckman) at 38,000 rpm ...
-
bioRxiv - Cell Biology 2019Quote: ... The protein solution was then centrifuged at 100,000 rpm in an Airfuge (Beckman Coulter) to remove aggregates ...
-
bioRxiv - Cell Biology 2019Quote: ... Intracellular proteins were then stained with anti-Gag KC57 RD1 antibody (1/100, Beckman Coulter) and/or anti-α-tubulin (5 μg/mL ...
-
bioRxiv - Neuroscience 2020Quote: ... aggregated proteins were removed by centrifugation at 30,000×g for 20 min (Beckman Coulter, USA). Protein was concentrated by centrifugal spin filters (Millipore ...
-
bioRxiv - Bioengineering 2023Quote: ... green fluorescent protein (GFP)-positive cells were FACS-sorted (MoFlo Astrios, Beckman Coulter, California, USA) as single cells into a 96-well plate and clonally propagated ...
-
bioRxiv - Genomics 2020Quote: The level of MPL protein on the platelet membrane was measured by flow cytometry (Beckman Coulter FC500) using the monoclonal antibodies ...
-
bioRxiv - Biochemistry 2019Quote: ... the soluble Tau protein was centrifuged at 60,000 rpm for 1 hour (Optima Max XP Beckman Coulter) to remove aggregates present if any and 20 μM of soluble Tau protein was incubated in 20 mM BES buffer pH 7.4 with 5 μM of Heparin 17,500 Da in presence of 25 mM NaCl ...
-
bioRxiv - Immunology 2020Quote: CCMV-SS and CCMV-Ctt830-SS VLPs were separated from cellular proteins by ultracentrifugation (SW28 rotor, Beckman, Palo Alto ...
-
bioRxiv - Microbiology 2023Quote: ... The surface expression level of S proteins (Figure 4B) was measured using CytoFLEX Flow Cytometer (Beckman Coulter) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
-
bioRxiv - Immunology 2024Quote: Antibodies and clones against the following proteins were used (clone, fluorophore, company): CD3 (UCTH1, PECy5, Beckman Coulter), CD7 (Horizon V450 or BUV395 ...
-
bioRxiv - Biochemistry 2020Quote: ... The dialysed proteins were clarified by centrifugation in an Optima MAX-XP tabletop ultracentrifuge (Beckman Coulter Life Sciences) (90,720 x g ...
-
bioRxiv - Cancer Biology 2022Quote: ... proteins enriched by IgG-agarose beads were eluted and processed with Agencourt AMPure-XP magnetic beads (Beckman Coulter) as previously described [40] ...
-
bioRxiv - Cell Biology 2020Quote: ... Unbound protein was separated from liposomes by transferring 100μl of this mixture to 7×20mm PC ultracentrifuge tubes (Beckman) and carefully layering an equal volume of 0.75M sucrose buffer followed by 25μl of sucrose-free HK buffer ...
-
bioRxiv - Cancer Biology 2020Quote: Cells expressing different fluorescent proteins were harvested from culture and counted using a ViCELL-XR (Beckman Coulter, Indianapolis, IN). Cells were washed 3 times in PBS to remove remaining cytokine ...
-
bioRxiv - Cell Biology 2019Quote: ... 50 μg of each standard protein were loaded on top of the gradient and centrifuged at 29.000 rpm in a SW41 rotor (Beckman) for 29 h at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: ... The gradients were loaded with 50 μg of recombinant CLASP2 C-term or protein standards of known sedimentation coefficient and centrifuged for 29 h at 29.000 rpm in a SW41 rotor (Beckman). Fractions of 700 μl were collected starting from the bottom of the tube using a peristaltic pump ...
-
bioRxiv - Systems Biology 2021Quote: ... Cells expressing the DsRed fluorescent protein were FAC sorted with a MoFlo cell sorter (Beckman Coulter GmbH, Krefeld, Germany) to obtain a highly enriched fraction for neuronal cells.
-
bioRxiv - Microbiology 2023Quote: ... The denatured protein fraction was then removed via ultracentrifugation at 100,000 g for 20 min at 4°C (Beckman Coulter Optima XE-90 – IVD ultracentrifuge with a 42.2 Ti rotor) ...
-
bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
-
bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 24 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 18 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
-
bioRxiv - Biochemistry 2021Quote: ... 300 μL of metabolite pool was aliquoted per target protein replicate and sealed with aluminum foil seals (Beckman Coulter 538619) to create the metabolite chambers ...