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Citations for Beckman :
1 - 50 of 64 citations for Water Nuclease Free Dist since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... DNA was eluted into nuclease-free water and purified with SPRIselect reagent (Beckman Coulter). All libraries were sequenced in one sequencing run on NextSeq 500 high-output (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μg/ml CHX) in nuclease-free water and centrifuged in a SW41Ti rotor (Beckman) for 2.5 hr at 40,000 rpm at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 100 µg/ml CHX) in nuclease-free water and centrifuged in a SW41Ti rotor (Beckman Coulter) for 2.5 hours at 40,000 rpm at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The sample was then diluted with nuclease-free water and cleaned twice with 1.5× vol of Agencourt RNAClean XP beads (Beckman Coulter, A63987). Immediately following this step ...
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bioRxiv - Plant Biology 2023Quote: ... All clean-up steps were performed using nuclease-free AMPure XP beads (Beckman Coulter) or Sera-mag SpeedBeads (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... The cDNA was eluted from the beads with 25 µL RNase-free water and purified again with 25 µL (1.0X volume) of Ampure XP beads (Beckman Coulter Life Sciences) then incubated at RT for 15 min then washed and dried as described above ...
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bioRxiv - Microbiology 2021Quote: ... RNA in the aqueous phase was purified and collected in 10 μL of RNase-free water using Agencourt RNAClean XP (Beckman Coulter, A63987) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... The fractions were washed two times with water (resuspended in water followed by 45 minutes at 42,000 r.p.m. using a 70 Ti Beckman rotor). Finally ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclease treated media was next transferred to 38-mL Ultra-Clear centrifuge tubes (Beckman Coulter) and centrifuged for 60 min at 133,000 x g at 4°C in a Sure Spin 630 36 mL rotor (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... USA) and Versalyse wash-free lysis (Beckman Coulter, USA).
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bioRxiv - Microbiology 2019Quote: Bacteria were enumerated in duplicate water samples by flow cytometry using a CytoFLEX (Beckman Coulter, Singapore) equipped with blue (488 nm ...
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bioRxiv - Neuroscience 2020Quote: ... sgRNA was purified using Ampure RNAse-free SPRI beads (Beckman-Coulter #A63987) and eluted in Ultrapure water (Invitrogen #10977–015) ...
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bioRxiv - Neuroscience 2020Quote: ... purifying with RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987), and eluting in Ambion nuclease-free water (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... After removal of free adaptors using Agencourt magnetic beads (Beckman Coulter, USA), the libraries were PCR-amplified for 12 cycles using primers compatible Illumina dual-index sequences ...
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bioRxiv - Genomics 2022Quote: ... Cell-free plasma was isolated using the Apostle MiniMax Kit (Beckman Coulter) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the casein-free whey was centrifuged at 100,000 g (Beckman Coulter, USA) for 105 min to precipitate the milk-exos ...
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bioRxiv - Neuroscience 2022Quote: ... sgRNA was purified using RNAse-free SPRI beads (Ampure RNAclean, Beckman-Coulter A63987), and eluted in Ultrapure water (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... 1X EDTA-free protease inhibitor cocktail) and ultra-centrifuged in SW40Ti rotor (Beckman) for 3.5 h at 170,920 x g at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... sgRNA was purified using RNAse-free SPRI beads (Ampure RNAclean, Beckman-Coulter A63987), and eluted in Ultrapure water (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... 1X EDTA-free protease inhibitor cocktail) and ultra-centrifuged in SW40Ti rotor (Beckman) for 3.5 h at 170,920 x g at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... sgRNA was purified using RNAse-free SPRI beads (Ampure RNAclean, Beckman-Coulter #A63987), and eluted in Ultrapure water (Invitrogen #10977–015) ...
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bioRxiv - Cell Biology 2020Quote: ... MTs were separated from free tubulin by centrifugation using an Airfuge (Beckman Coulter) for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mM Tris-HCl in DEPC-treated water) in a thick-walled ultracentrifuge tube (Beckman Coulter, Cat # 355631) and spun at 107,000 RCF ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... Nuclei were collected and resuspended in 150 µL water and combined with 300 μL AMPure XP beads (Beckman). DNA-end repair with T4 DNA Polymerase (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... and adding this to 3 ml sterile inoculum water (Beckman Coulter, Inc. Brea, CA, cat no B1015-2) and 840 μl phenol red solution ...
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bioRxiv - Neuroscience 2021Quote: ... which was followed by purification with Agencourt RNAclean XP (RNAse free) beads (Beckman Coulter). Amplified RNA was fragmented with fragmentation buffer and purified using the RNA cleanXP beads ...
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bioRxiv - Neuroscience 2020Quote: ... sgRNA was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter #A63987), and eluted in Ambion nuclease-free water (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... The fiber-free supernatant was then centrifuged at 100,000g with tabletop ultracentrifuge (Beckman Coulter) for 70 min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The flasks were cooled in an ice water bath and cell cultures were centrifuged in a rotor JA-25.50 (Beckman) at 4°C and 4000 x g for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... water was added to a total volume of 50 μl and reactions were purified using AMPure XP beads (Beckman Coulter) with a 0.9X ratio according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Microbial cells were collected from to 2-3 L of water samples by centrifugation at 12,000 × g and 4°C for 20 min in a high-speed centrifuge (Beckman, USA). Total genomic DNA was extracted using AxyPrep™ Genomic DNA Miniprep Kit (Axygen ...
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bioRxiv - Plant Biology 2023Quote: ... and water to a final volume of either 2 μL for the acoustic liquid handling robot (Labcyte Echo 550, Beckman) or 5 μL for manual handling ...
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bioRxiv - Neuroscience 2020Quote: ... We purified the transcription products using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and eluted them in Ambion nuclease-free water (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
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bioRxiv - Microbiology 2020Quote: Sulfate concentrations in interstitial water samples of microcosms were determined by capillary electrophoresis (P/ACETM MDQ molecular characterization system, Beckman Coulter) with the CEofixTM anions 5 kit (Analis) ...
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bioRxiv - Cancer Biology 2020Quote: ... The emulsions were then broken using perfluoro-1-octanol and the aqueous fraction was diluted in water and collected for DNA purification with SPRI beads (Beckman Coulter). Sample indexes and Illumina adaptor sequences were then added via a 10 cycle PCR reaction and the amplified material was then SPRI purified a second time ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT and 10 mM Tris-HCl in DEPC-treated water was added to the bottom of the lysis solution in ultracentrifuge tubes (Beckman Coulter) to form a gradient ...
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bioRxiv - Genomics 2023Quote: ... After a subsequent wash step with ice cold 70 % ethanol the resulting DNA pellet was resuspended with 100 μL PCR-grade water followed by further purification via solid-phase reversible immobilization using 1.5 volume of AMPure XP Beads (Beckman Coultier™) and final elution in 20 μL 1× TE ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatant was incubated in a boiling water bath for 5 min and centrifuged for 10 min at 40,000 rpm (Beckman Ti45 rotor). Supernatant was supplemented with ammonium sulfate (1 g per 6.1 ml) ...
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bioRxiv - Biochemistry 2021Quote: Cell free BAL was weighed (=400-800 µL) and ultra-centrifuged (J2-MC, Beckman Coulter, Krefeld, Germany) for 1h at 4°C at 38.730 g ...
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bioRxiv - Microbiology 2020Quote: ... Unsieved soil samples were suspended in water and analyzed on a LS 13 320 Series Laser Diffraction Particle Size Analyzer (Beckman Coulter Brea CA 92821). Particle sizes were grouped according USDA soil texture classification ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM HEPES pH 7.4 and EDTA free protease inhibitor were prepared in polyallomer optiSeal ultracentrifuge tubes (11.2 ml capacity; Beckman Coulter) gradients were allowed to diffuse 1h at 20 °C ...
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bioRxiv - Biophysics 2020Quote: ... GLIC proteoliposomes were collected by centrifugation of the Biobead-free sample at 100,000 rpm in TLA-120.2 rotor (Beckman Coulter) for 1 hr ...
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bioRxiv - Microbiology 2024Quote: The resulting amplicons were then cleaned of free primers and primer dimer species using AMPure XP beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT library preparation kit (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... Then 0.8 mL of Cold 60% sucrose buffer A was added to RNAse-free 5 mL Ultraclean tubes for ultracentrifugation in a SW55Ti (Beckman). The ribosome-containing supernatant was used to fill these tubes and an ultracentrifugation step was performed for 16 hs at 150.000g ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Developmental Biology 2022Quote: ... TH concentrations are measured by a medical laboratory in Perpignan (Médipole) using an ELISA kit (Access Free T3, T4, Beckman Coulter).
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bioRxiv - Molecular Biology 2019Quote: ... Rnase-free sucrose) and gradients were centrifuged at 35,000 rpm for 3 hours 20 minutes using a SW41Ti rotor (Beckman Coulter). Absorbance was followed at 254 nm ...
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bioRxiv - Neuroscience 2022Quote: ... DNase-treated RNA was subsequently purified using RNase-free Agencourt AMPure XP beads (Beckman Coulter; 2:1 volume of beads:sample).
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bioRxiv - Immunology 2023Quote: ... The ratio of Ca2+-bound Indo-1 versus Ca2+-free Indo-1 was analyzed using FlowJo 9 software (Beckman Coulter).