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Citations for Beckman :
1 - 50 of 86 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... SPRI (solid-phase reversible immobilization, Beckman Coulter) cleanup was performed after ligation ...
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bioRxiv - Developmental Biology 2019Quote: ... Solid Phase Reversible Immobilization (SPRI) beads (Agencourt AMPure XP, Beckman Coulter) were used at a 1.8X beads ratio ...
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bioRxiv - Plant Biology 2022Quote: ... followed by DNA purification using solid phase reversible immobilization (SPRI) beads (Beckman Coulter Life Sciences ...
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bioRxiv - Immunology 2020Quote: ... Two rounds of 0.6X solid-phase reversible immobilization beads (AmpureXP, Beckman, or CleanNGS, Proteigene) cleaning were used to purify 100 μl of pooled cDNA with final elution in 15 μl PCR-grade H2O (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA purification was performed by solid-phase reversible immobilization using AMPure XP (Beckman Coulter) magnetic beads ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting cDNA libraries were purified with solid-phase reversible immobilization SPRI beads (Beckman) and checked on a BioAnalyzer High sensitivity chip (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting ATAC libraries were purified with solid-phase reversible immobilization SPRI beads (Beckman) and checked on a BioAnalyzer High sensitivity chip (Agilent ...
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bioRxiv - Bioengineering 2023Quote: ... purified by solid phase reversible immobilization beads (Agencourt AMPure XP, Beckman Coulter, cat# A63882). Sequencing was performed on an Illumina NextSeq 500 platform.
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were then purified via solid phase reversible immobilization (SPRI) using AMPure XP beads (Beckman Coulter) to remove fragments less than 100 bp ...
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bioRxiv - Immunology 2022Quote: ... purified with two rounds of 0.6X solid-phase reversible immobilization beads (AmpureXP, Beckman, or CleanNGS, Proteigene) and finally eluted in nuclease free water ...
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bioRxiv - Immunology 2022Quote: ... followed by purification with Agencourt AMPure XP solid-phase reversible immobilization (SPRI) beads (Beckman Coulter #A63881). DNA was quantified using a Qubit dsDNA High Sensitivity Assay Kit (Life Technologies #Q32851 ...
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bioRxiv - Immunology 2019Quote: ... Amplified libraries were purified using AMpure XP solid-phase reversible immobilization beads (Beckman Coulter Inc., Indianapolis, IN) and quantified using a high sensitivity DNA Qubit assay (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... DNA size selection was performed using solid-phase reversible immobilization (SPRI) beads (AMPure XP [Beckman Coulter, A63881]) at an SPRI to DNA ratio of 0.5 ...
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bioRxiv - Immunology 2020Quote: ... The resulting library was purified with 0.8X solid-phase reversible immobilization beads (AmpureXP, Beckman, or CleanNGS, Proteigene). Libraries generated from multiple 96-well plates of single cells and carrying distinct i7 barcodes were pooled for sequencing on an Illumina NextSeq550 platform ...
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bioRxiv - Microbiology 2019Quote: ... Extracted DNA was purified using AMPure XP solid-phase reversible immobilization (SPRI) beads (Beckman Coulter, High Wycombe, UK), eluted in 26 µl of TE buffer and quantified using a Qubit 2.0 fluorometer (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... The size selection was performed using solid-phase reversible immobilization (SPRI) beads (Agencourt AMPure XP Beads) from Beckman Coulter ...
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bioRxiv - Genomics 2021Quote: ... The size selection was performed using solid-phase reversible immobilization (SPRI) beads (Agencourt AMPure XP Beads) from Beckman Coulter ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The pooled samples were purified and size-selected using solid-phase reversible immobilisation beads (Beckman Coulter, Inc. CA). We used the Agilent Bioanalyzer 2100 system to assess the quality of the pooled library by the obtention of a single clear band of 420 bp ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from single cells using RNAClean XP Solid Phase Reversible Immobilization (SPRI) beads (Agentcourt, Beckman Coulter), and hybridized first using RT primer (5′/5Biosg/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3′ ...
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bioRxiv - Immunology 2022Quote: ... RNA from single-sorted cells was extracted using RNAClean XP Solid Phase Reversible Immobilization (SPRI) beads (Beckman Coulter). Extracted RNA was first hybridized using an RT primer (/5BiosG/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN) ...
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bioRxiv - Microbiology 2020Quote: The amplified ATAC-seq libraries were immediately purified and size selected with a double-sided solid-phase reversible immobilization (SPRI) approach (0.5x/1.4x) using AMPure XP beads (Beckman Coulter) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Each amplicon was purified with solid phase reversible immobilization (SPRI) paramagnetic bead-based technology (AMPure XP beads, Beckman Coulter) with a Bead:DNA ratio of 0.7:1 (v/v ...
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bioRxiv - Genetics 2020Quote: ... PCR products were purified using the AMPure XP solid-phase reversible immobilization kit (SPRI; Beckman Coulter, cat. no. A63881) and subjected to Illumina next-generation sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... Pooled purification of the PCR amplicons was then performed using Solid Phase Reversible Immobilisation (SPRI) beads (Beckman Coulter, Singapore). Purified PCR amplicons were sequenced via next-generation sequencing (NGS ...
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bioRxiv - Genomics 2020Quote: ... The size selection for all the libraries were done using solid-phase reversible immobilization (SPRI) beads (Agencourt AMPure XP Beads) from Beckman Coulter ...
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bioRxiv - Bioengineering 2021Quote: ... and pooled into 100 µl reactions for Solid Phase Reversible Immobilization (SPRI) cleanup (1X) using AMPure XP beads according to the manufacturer’s instructions (Beckman Coulter). Each 100 µl starting product was eluted into 5 µl of water ...
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bioRxiv - Plant Biology 2021Quote: ... The pool was then purified using the solid phase reversible immobilization (SPRI) Agencourt AMPure XP kit (Beckman Coulter, Milan, Italy).
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bioRxiv - Microbiology 2021Quote: ... followed by purification with a 2.2:1 volumetric ratio of RNAClean XP solid phase reverse immobilization (SPRI) beads (A63987, Beckman Coulter). Copy numbers of resulting cDNAs were determined by limiting-dilution PCR using fluorescence-assisted clonal amplification (FCA ...
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bioRxiv - Synthetic Biology 2024Quote: ... Double-stranded DNA was purified by solid phase reversible immobilization (SPRI) bead cleanup using AMPure XP beads (Beckman Coulter #A63881). Beads were added in 1.8:1 volume:volume ratio to PCR template and isolated per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The 40 µL elution was further purified and size-selected using magnetic solid phase reversible immobilization (SPRI) beads following the manufacturer’s instructions (Beckman Coulter, A63880) and eluted in 20 µL ...
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bioRxiv - Genomics 2023Quote: ... After a subsequent wash step with ice cold 70 % ethanol the resulting DNA pellet was resuspended with 100 μL PCR-grade water followed by further purification via solid-phase reversible immobilization using 1.5 volume of AMPure XP Beads (Beckman Coultier™) and final elution in 20 μL 1× TE ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product (barcoded 16S amplicon) was cleaned up from the PCR reaction mixtures using AMPure XP Solid Phase Reversible Immobilization (SPRI) paramagnetic beads (Beckman-Coulter #A63880). 50 µL of the PCR reaction mixture containing the amplified product was mixed with equal volume of SPRI bead suspension and a magnetic separation rack was used to separate DNA-bound beads from rest of the solution ...
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bioRxiv - Genomics 2021Quote: ... Potential contaminating DNA was removed by digestion with rDNase then RNA was purified using Beckman Coulter’s solid-phase reversible immobilization (SPRI) paramagnetic beads (Agencourt AMPure XP, Beckman Coulter, Brea, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... products within the 200-400 bp size range were selected by solid phase reversible immobilization with the Agencourt AMPure XP reagent (Beckman Coulter, C.A., U.S.A.) and PCR amplification consisted of 12 cycles ...
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bioRxiv - Bioengineering 2024Quote: PCR amplicons of the CAR and mCherry transgenes were generated as described previously.34 PCR products were pooled into 600 μL and were purified using a singular round of Solid Phase Reversible Immobilization (SPRI) cleanup using AMPure XP beads (Beckman Coulter, Brea, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... These reactions were then pooled into 600 uL reactions for Solid Phase Reversible Immobilization (SPRI) cleanup (1x) using AMPure XP (Cat # B23318, Beckman-Coulter, Brea, CA) beads according to the manufacturer’s instructions and eluted at 2 mg/mL in DNAase free water ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The upper aqueous phase was further processed for total RNA extraction using the Agencourt RNAdvance Tissue Kit (Beckman Coulter, Germany) on a KingFisher Flex Purification System (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2021Quote: ... 561 nm and 640 nm solid state lasers (Beckman Coulter). Data analysis was performed using FlowJo v10.6.1 software following compensation with single color controls.
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bioRxiv - Bioengineering 2019Quote: ... The organic phase was removed and discarded while the remaining aqueous phase was purified using AMPure XP beads (Beckman Coulter, A63881). DNA was eluted in 50 μL of water.
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bioRxiv - Molecular Biology 2022Quote: ... a reverse-phase 0.65x AMPure XP beads (Beckman Coulter) DNA purification step was done ...
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bioRxiv - Bioengineering 2020Quote: ... 37 °C and further purified by reversed-phase HPLC (Beckman System Gold) run on a semi-preparative reversed-phase C18 column (Vydac ...
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bioRxiv - Biochemistry 2023Quote: ... The extraction was centrifuged at 39,000 × g (Beckman) for 60 minutes to remove insoluble components ...
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bioRxiv - Biochemistry 2024Quote: ... The extraction was centrifuged at 39,000 g (Beckman) for 1 hour to remove the insoluble components ...
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bioRxiv - Biochemistry 2019Quote: ... The Cherenkov-radiation in the KOH-phase was determined in a β-counter (Beckman), using 3H-settings ...
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bioRxiv - Genetics 2022Quote: ... the aqueous phase was processed using the Agencourt® RNAdvance Tissue kit (Beckman Coulter) and the KingFisher Flex system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... before extraction of RNA using RNAClean XP beads (Beckman). RNA aliquots were the quantified for HIV Gag RNA using TaqMan Fast Virus 1-step master mix (Thermo ...
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bioRxiv - Plant Biology 2021Quote: ... and extraction using AMPure XP beads (Beckman Coulter, Cat. #A63881). DNA concentration and absorbance were measured with a spectrophotometer (NanoDrop OneC Microvolume UV-Vis Spectrophotometer with WiFi ...
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bioRxiv - Genomics 2022Quote: ... The aqueous phase was then incubated for 30 minutes with 1x AMPure XP beads (Beckman Coulter, A63881), placed on a magnetic stand and washed twice with 80% ethanol before eluting the DNA in 30 μL of nuclease-free water ...
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bioRxiv - Cancer Biology 2021Quote: ... The partially purified MsDef1 was further purified by reverse-phase HPLC column chromatography (Beckman Coulter, Brea, CA) using a reverse phase C18 column (Delta Pak Wat 011793 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA extractions were done using an Agencourt DNAdvance kit (Beckman-Coulter A48705). Library preparation was done using a diluted Nextera kit (Illumina FC-131-1024 ...