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Citations for Beckman :
1 - 50 of 175 citations for Simian Virus 40 Large Tumor Antigen Amino Terminal Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Large fragments were removed using 0.4 × AMPure beads (Beckman Coulter). Libraries were sequenced on NextSeq 500.
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bioRxiv - Genomics 2021Quote: ... large fragments were removed with 0.5X vol AMPure XP (Beckman Coulter #A63880) purification ...
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bioRxiv - Genomics 2020Quote: ... and then large fragments (above 1000 bp) removed with 1X AMPure beads (Beckman Coulter). The concentration of libraries was measured with Qubit dsDNA HS Assay (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... HIV-1 p24 core antigen KC57: 6604667 from Beckman Coulter and APC HIV-1 (p24 ...
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bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Primer dimers and large >1,000 bp fragments were removed using AMPure XP beads (Beckman Coulter). The quality of libraries was checked on Agilent High Sensitivity DNA Bioanalysis and quantified by qubit ...
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bioRxiv - Microbiology 2023Quote: ... and HIV-1 core antigen-FITC (KC57, Beckman Coulter; 6604665).
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bioRxiv - Immunology 2023Quote: ... Surface antigen expression was measured with the CytoFLEX LX (Beckman Coulter) and data was analyzed with the CytExpert Software Version 2.4 (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... staining with anti-HIV-1 Core Antigen Clone KC57 FITC (Beckman Coulter) and detection of HIV-1-infected cells by flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... Cells were probed for HIV-1 core antigen-FITC KC57 (Beckman Coulter) for 1 hr at 4°C then washed with PBS and resuspended in FACS buffer (5% FBS in PBS) ...
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bioRxiv - Microbiology 2020Quote: ... and p24 antibodies (anti-HIV core antigen: clone KC57, Beckman Coulter, 1:200).
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bioRxiv - Cancer Biology 2022Quote: ... Antigen cross-presentation was assessed using a Cytoflex S flow cytometer (Beckman Coulter) and was measured as the percentage of APC+ cells within CD45highCD11b+ MdCs and CD45lowCD11b+ MG ...
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bioRxiv - Bioengineering 2021Quote: ... or 7-Amino-Actinomycin D (7-AAD, Beckman Coulter, B88526) was added to the cell suspensions ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and filtered through 0.22 µm filters to remove large sized vesicles and further centrifuged (Beckman Coulter Optima Ultracentrifuge ...
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bioRxiv - Microbiology 2024Quote: ... The virus was enriched by ultracentrifugation (Beckman Coulter Optima™ L-80XP) at 246,347g for 4 hours at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The dyes used were: KC57 anti-HIV-1 core antigen-PE (Beckman coulter CO604667), Live/Dead fixable Far Red Dead Cell Stain Kit (Invitrogen L10120 ...
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bioRxiv - Microbiology 2021Quote: ... PE) and HIV-1 Gag core antigen (FH190-1-1, Beckman Coulter; PE, FITC). All samples were acquired on either an BD Fortessa X20 or LSR II using BD FACSDiva software and analyzed using FlowJo v10 (Tree Star) ...
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bioRxiv - Genomics 2019Quote: ... Samples were size selected using a 0.5X Ampure XP cleanup to remove large fragments followed by 3 consecutive 1.5X Ampure XP (Beckman Coulter # A63880) bead cleanup followed by a final 1.2X Ampure XP cleanup to completely remove all residual primers and resuspended in a final volume of 20 µL EB using the manufacturer’s recommended protocol.
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bioRxiv - Cancer Biology 2023Quote: ... Large EVs were collected by centrifugation at 10,000 x g for 30 minutes in a Ti 45 rotor (Beckman Coulter). Small EVs were collected by a modified cushion-density gradient protocol (Li et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% NP-40) into centrifugation tubes (Beckman), 331372 ...
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bioRxiv - Cell Biology 2020Quote: ... thin-layer cell preparations for confocal microscopy of enriched small luteal cells and large luteal cells was performed using Cytofuge 2 (Beckman Counter). Cells were then fixed with 10% neutral buffered formalin at 4 °C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... then filtered through a 0.45 μm filter to remove large debris and then centrifuged at maximum speed using a SW 28 Ti Swinging-Bucket Aluminum Rotor (Beckman Coulter) for 2-4 h ...
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bioRxiv - Cell Biology 2019Quote: ... Viral supernatants were collected and large particulates were pelleted by centrifugation at 3000 RPM in a swinging bucket rotor (Beckman Coulter). Cleared supernatants were filtered through a 0.45 um syringe filter (Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA library was purified and size selected using a double-sided bead purification protocol with AMPure XP beads that removes both unwanted small and large fragments (Beckman Coulter). The QuBit dsDNA HS Assay (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... the libraries were further purified using the SPRIselect reagent to remove large DNA molecules (a right-side size selection with 0.55X followed by 1.5X ratios of beads) (Beckman Coulter, Brea, California). On average 10.6 millions of 38-nucleotide paired-end sequences were obtained using a NextSeq 500 sequencer (Illumina).
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bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2020Quote: Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 28) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor) for 2h at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and filtered through 0.22 µm filters to remove large sized vesicles and further centrifuged (Beckman Coulter Optima Ultracentrifuge, Beckman Coulter, Brea, CA) at 100,000 g at 4°C for 120 minutes with a Type 70 Ti rotor to pellet sEVs ...
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bioRxiv - Genomics 2023Quote: ... 40 µL of Ampure XP beads (Beckman Coulter) was added to every well ...
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bioRxiv - Immunology 2023Quote: ... 40 and 60% iodixanol in Optiseal tubes (Beckman) followed by centrifugation at 350,000g using a Type 70 Ti ultracentrifuge rotor (Beckman) ...
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bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... Double-sided magnetic bead based DNA purification (to remove primer dimers and large >1,000 bp fragments) was performed using Agencourt AMPure XP beads (Beckman Coulter, High Wycombe, UK). DNA was quality controlled using an Agilent 2100 Bioanalyzer (Agilent ...
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bioRxiv - Genomics 2019Quote: ... Membranes and other cellular debris were sedimented away from soluble protein on a Beckman J2-21 large floor centrifuge (Beckman Coulter, Brea, CA). Supernatant containing the recombinant protein was incubated with 10 mL of precleared Ni-NTA beads for 10min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... was then added to the product which passed microfluidizer and unbroken cells and large debris were eliminated by centrifugation at 4000 × g (Beckman Coulter™, USA). The supernatant containing the IMVs was collected and washed several times with resuspension buffer by centrifugation at approximately 310,000 × g at 4°C for 30 minutes (SORVALL® Centrifuges ...
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bioRxiv - Microbiology 2019Quote: ... run in a SW-40 Ti rotor (Beckman Coulter) at 57471 × g for 16.5 h and analyzed using a Gradient Station (Biocomp ...
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bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... and 100,000 g for 70 min (Beckman, SW 40 rotor). The obtained pellets were resuspended in cold PBS and ultracentrifuged at 100,000g for an additional 70 min ...
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bioRxiv - Biophysics 2019Quote: Sedimentation velocity data of the N-terminal RBCC domain of KAP1 (0.5 mg/mL) were obtained using a Beckman Optima XL-I analytical ultracentrifuge (Beckman Coulter) with an absorbance optical system ...
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bioRxiv - Cancer Biology 2023Quote: ... 20,000 CD45+DAPI− live tumor-infiltrating leukocytes were isolated by using a MoFlo Astrios EQ jet-in-air sorting flow cytometer (Beckman Coulter ...
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bioRxiv - Biochemistry 2021Quote: ... The virus-containing filtrate was then further purified using an ultracentrifuge (Optima XPN-90 Ultracentrifuge, Beckman Coulter, USA) with a cycle of 1,000 rpm (116 g ...
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bioRxiv - Molecular Biology 2020Quote: ... Gradients were spun in an SW 40 Ti rotor (Beckman Coulter) at 202048 × g for 4 h and fractionated at a BioComp Gradient Station ip using a Triax Flow Cell for UV measurement.
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bioRxiv - Biochemistry 2023Quote: ... sonicated and centrifuged at 40 000 rpm for 45 minutes (Beckman L-90 ultracentrifuge using a Ti70 rotor) ...
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bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
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bioRxiv - Microbiology 2023Quote: ... PBS-washed cells were PFA-fixed for 90 minutes and immunostained with the following antibodies diluted in 0.1% Triton X-100 in PBS: anti-HIV-1-core-antigen-FITC or -PE (#6604665 or #6604667; Beckman Coulter), rabbit-anti-human-MX1/2 (#sc-166412 ...
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bioRxiv - Cell Biology 2021Quote: ... BM analyses were terminal analyses at the time of tissue harvest and BM cellularity was determined using a ViCell automated cell counter (Beckman-Coulter).
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bioRxiv - Genetics 2023Quote: ... STR DNA fragments were amplified using a pair of specific primers with 5’ terminal parts of P5 and P7 Illumina adapters (Table S1) and purified by 100% (v/v) SPRIselect beads (Beckman Coulter). In the second step ...
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bioRxiv - Cancer Biology 2024Quote: ... CD138 and CD70 for CAR-T (see table of antibodies) and tumor identification and analyzed by flow cytometry using a CytoFLEX Flow Cytometer (Beckman Coulter). Data was analyzed using FlowJo 10.10.0 software ...
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bioRxiv - Microbiology 2020Quote: ... The virus was then purified over a 20% sucrose cushion by centrifugation at 35,000 rpm (Beckman Coulter SW41 rotor) for 3 h at 4°C ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...