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Citations for Beckman :
1 - 50 of 314 citations for SARS CoV 2 Nucleoprotein His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: CD27+ IgM- B cells cross-binding to SARS-CoV-1 and SARS-CoV-2 RBD were sorted from SARS-CoV-2 vaccinated SARS convalescents with MoFlo Astrios EQ Cell Sorter (Beckman Coulter). Briefly ...
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bioRxiv - Immunology 2020Quote: ... Access SARS-CoV-2 IgG (Beckman Coulter), and LIAISON SARS-CoV-2 S1/S2 IgG (DiaSorin) ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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bioRxiv - Immunology 2021Quote: The ACCESS SARS-CoV-2 CLIA (Beckman Coulter Inc., California, USA) was used for semiquantitative detection of IgG directed against S protein RBD using serum obtained from venipuncture blood ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 RBD+ were sorted with MoFlo Astrios EQ Cell Sorter (Beckman Coulter).
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 RBD+ were sorted with MoFlo Astrios EQ Cell Sorter (Beckman Coulter). FACS data were analyzed using FlowJo™ v10.8 (BD Biosciences).
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bioRxiv - Bioengineering 2021Quote: ... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
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bioRxiv - Immunology 2023Quote: The SARS-CoV-2 lentiviral pseudotyped neutralization assays were performed on integrated automation platforms consisting of a Biomek liquid handler from Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... Beckman Coulter Access SARS-CoV-2 IgG assays (Brea, CA, USA) targeting the RBD were performed on UniCel DxI 800 Access Immunoassay System (Beckman Coulter). Index value threshold for positivity was 1 as recommended ...
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bioRxiv - Genomics 2019Quote: ... the Hi-C libraries were purified with AMPure XP beads (Beckman Coulter). The concentration of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... the Hi-C libraries were purified with AMPure XP beads (Beckman Coulter). Quantity and integrity of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies).
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bioRxiv - Systems Biology 2021Quote: HeLa Kyoto and R1/E mESCs (gifts from Hyman lab, Dresden) and HEK293 (gift from Beckman lab, Berlin), Ptk2 cell lines (gift from Simons lab ...
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bioRxiv - Genetics 2019Quote: ... The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter, A63881) and sequenced using an Illumina sequencing platform.
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bioRxiv - Genetics 2022Quote: ... and the final Hi-C library was purified with AMPure XP beads (Beckman Coulter). Promoter Capture Hi-C was performed using a customdesign Agilent SureSelect system following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter). Test sera were diluted 1:40 in growth medium and serially diluted ...
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bioRxiv - Genomics 2023Quote: ... and Hi-C template DNA was purified using 1x AMPure XP beads (Beckman Coulter, A63881) and stored at 4°C until library preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibody derived tags (ADTs) and cDNA were separated using the Ampure Beads (Beckman Coulter, USA) and constructed libraries dependently ...
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bioRxiv - Immunology 2021Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2022Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted to a DNA concentration of 50 ng/µl followed by ligation of 454 sequencing adaptors and molecular identifier (MID) tags using the SPRIworks Fragment Library System II for Roche GS FLX+ DNA Sequencer (Beckman Coulter), excluding fragments smaller than 350 base pairs ...
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bioRxiv - Immunology 2022Quote: ... Targeted amplification was performed for 13 cycles and the products were separated according to size into <400 bp (DNA barcode-tags) and >400 bp (the TCR sequences) using 0.6x SPRIselect beads (Beckman Coulter, B23318). Separate processing of the >400 bp bead-bound TCR sequences and the <400 bp in solution DNA barcodes was conducted according to manufacturer’s instruction and the TCR amplification products were sequenced on a NovaSeq running a 150 paired-end program ...
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bioRxiv - Plant Biology 2019Quote: ... The corresponding TAG spots were scraped and quantified for radioactivity using a LS 6500 multi-purpose scintillation counter (Beckman-Coulter, Mississauga, Canada).
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bioRxiv - Immunology 2022Quote: ... and mRNA PCR products were separated from sample tag and AbSeq products with double-sided size selection using Agentcourt® AMPure® XP magnetic beads (Beckman Coulter, #A63880). mRNA and Sample Tag products were further amplified ...
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bioRxiv - Neuroscience 2024Quote: ... 20.000 rpm for 2 hours (Beckman). Collected viral particles were then suspended in DMEM and stored to –80°C ...
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bioRxiv - Cell Biology 2022Quote: ... and the post-nuclear supernatant was loaded onto an 8-step discontinuous sucrose density gradient (HEPES-buffered 0.2–2 M sucrose) and centrifuged at 55,000 rpm for 2h at 4 °C using an MLS50 rotor (Beckman Coulter). Extracellular acidification rate (ECAR ...
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bioRxiv - Immunology 2019Quote: ... Receptor (R; Beckman Coulter, Z199, GL183, CD158e1/2) and KIR2DS4 (S4 ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) and centrifuged for 2 hours and 15 minutes at 39000 rpm at 4°C (Beckman Coulter TLS55 rotor). Fractions corresponding to the large mitochondrial subunit were collected and subjected to buffer exchange (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... calibrating with 2-micron latex bead standard (Beckman Coulter) diluted in 18% BSW as the electrolyte ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II ...
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bioRxiv - Immunology 2022Quote: ... Tagmented DNA was isolated using 2× SPRI Beads (Beckman Coulter) and amplified via PCR ...
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bioRxiv - Biochemistry 2020Quote: ... Fragments were purified using 2 volumes AMPure XP beads (Beckman Coulter) and amplified for 8-10 cycles using NEBNext Multiplex Oligos ...
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bioRxiv - Biochemistry 2021Quote: ... 6 mM 2-mercaptoethanol and centrifuged with a 70Ti (Beckman coulter) rotor at 40,000 rpm for 18 hours at 4°C ...
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bioRxiv - Genomics 2022Quote: ... and purified with 2 rounds of AMPure XP beads (Beckman Coulter). Libraries were sequenced on an Illumina NextSeq 550 ...
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bioRxiv - Biochemistry 2020Quote: ... Fragments were purified using 2 volumes AMPure XP beads (Beckman Coulter) and amplified for 8-10 cycles using NEBNext Multiplex Oligos ...
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bioRxiv - Genomics 2023Quote: ... prepare sucrose gradient in 2 ultracentrifuge buckets (Beckman Coulter cat: 344058) by layering 5 mL of Interphase buffer atop 10 mL of SCB in each ...
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bioRxiv - Molecular Biology 2019Quote: ... the VLPs were pelleted for 2 h at 40,000 rpm (Beckman TLA55) at 4°C and subsequently resuspended in PBS (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 mM DTT) with 0.7M sucrose in ti-45 tubes (Beckman-Coulter). Ribosomes were pelleted in a Ti-45 rotor at 27000 rpm for 16 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... using an An50Ti rotor and standard 2-channel epon centerpieces (Beckman-Coulter). All the samples were measured in the same run and at multiple wavelengths (278 nm ...
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bioRxiv - Biophysics 2020Quote: ... VLPs were pelleted for 2 h at 40,000 rpm (Beckman TLA55 rotor) at 4°C and solved in PBS (pH 7.4).
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bioRxiv - Genomics 2021Quote: ... Libraries were purified with 2 rounds of Ampure XP beads (Beckman-Coulter) and evaluated on a 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: ... using an An50Ti rotor and standard 2-channel epon centerpieces (Beckman-Coulter). All samples were measured in the same run ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μL of the compounds were added automatically (Beckman FX 96 tip) to 200 μL media to make it up to the final concentration for each drug ...
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bioRxiv - Immunology 2020Quote: ... After adding 2 mL of VersaLyse solution (Beckman Coulter, catalog number A09777) with 2.5% Fixative Solution (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... all from BD Biosciences as well as IL-2 PE (clone N7.48A, Beckman, Cat IM2718U) and TNF PCP-Cy5.5 (clone MAb11 ...
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bioRxiv - Biochemistry 2024Quote: ... using an An60Ti rotor and standard 2-channel epon centerpieces (Beckman-Coulter). Measurements were performed at 4 °C ...
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bioRxiv - Biochemistry 2019Quote: ... followed by spinning at 158,000 g for 2 hours (Ti45 rotor, Beckman Coulter). The lysate was passed through a column containing 2 mL TALON superflow at 0.5 mL/min ...
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bioRxiv - Microbiology 2020Quote: ... Centrifugation was performed at 120,000 × g for 2 hr (Beckman TLS 55 rotor), after which the interface between 0.25 M and 1.1 M sucrose was collected and resuspended in 1 ml 19% OptiPrep for a step gradient containing 0.5 ml 22.5% ...
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bioRxiv - Cell Biology 2022Quote: ... 2× – G2) was interrogated using a using a Gallios flow cytometer (Beckman Coulter) and subsequent data analysis with FlowJo (v.10.8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged at 1500 rpm for 2 minutes (Beckman Allegra X15R, 524xg), supernatant was removed and tissue was carefully triturated using fire-polished glass pipettes with 2 mL pre-warmed media ...
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bioRxiv - Genomics 2019Quote: ... Tagmented DNA was purified using 2× SPRI beads (Agencourt AMPure XP-Beckman Coulter). Finally ...