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Citations for Beckman :
1 - 50 of 495 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Neuroscience 2022Quote: ... APC-tagged anti-CD41 (Beckman Coulter).
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bioRxiv - Cell Biology 2024Quote: ... k factor 81.3 (Beckman Coulter). RNA was isolated from the pelleted samples with a miRNeasy micro kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... The immunoturbidimetric test was used to assess serum complement (C) and immunoglobulin antibodies (AU5421; Beckman Coulter, USA). Immunoblotting and immunofluorescence were used to detect serum autoantibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Centrifugations at 10,000 g (k-factor = 2547.2) and 100,000 g (k-factor = 254.7) were done at +4°C using polyallomer tubes (Beckman Coulter Inc. ...
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bioRxiv - Immunology 2020Quote: ... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... recombinant protein and anti-CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Genomics 2019Quote: ... the Hi-C libraries were purified with AMPure XP beads (Beckman Coulter). The concentration of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... the Hi-C libraries were purified with AMPure XP beads (Beckman Coulter). Quantity and integrity of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies).
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bioRxiv - Cell Biology 2020Quote: ... EVs were ultracentrifuged (Type 45 Ti rotor, k-Factor 133, Beckman Coulter) with 100000 g for 90 min 4°C ...
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bioRxiv - Microbiology 2019Quote: ... performed ultracentrifugation (40,000 rpm, either 4 h or 18 h, +4 °C, SW41 rotor, Beckman Coulter), and collected approximately 600 μl fractions by puncturing the tubes with a thin (23G or 25G ...
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bioRxiv - Biochemistry 2021Quote: ... 1 h 10 min (45Ti, Beckman), resuspended in lysis buffer and stored in aliquots at –80°C until use ...
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bioRxiv - Genetics 2019Quote: ... The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter, A63881) and sequenced using an Illumina sequencing platform.
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bioRxiv - Genetics 2022Quote: ... and the final Hi-C library was purified with AMPure XP beads (Beckman Coulter). Promoter Capture Hi-C was performed using a customdesign Agilent SureSelect system following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The tagged PCR products were then purified with the Agencourt AMPure XP kit (Beckman Coulter, Switzerland) and eluted in 30 µL DNA/RNA-free water ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... fixed angle Type 45 Ti rotor (k factor = 133; Beckman Coulter, Miami, FL, USA), at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions were subjected to SDS-PAGE and Coomassie blue staining.
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions ...
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bioRxiv - Genomics 2023Quote: ... and Hi-C template DNA was purified using 1x AMPure XP beads (Beckman Coulter, A63881) and stored at 4°C until library preparation ...
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bioRxiv - Neuroscience 2020Quote: ... for 4 h with SW28 rotor (Beckman Coulter). The concentrated vector was stored at −80 °C until use ...
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bioRxiv - Plant Biology 2021Quote: ... 4 °C for 18 h (Beckman SW41 rotor). The sugar solution contains 0.65 M sucrose ...
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bioRxiv - Microbiology 2021Quote: ... Growth rates were monitored for 96 h and cell densities were determined every 24 h using a Coulter Counter Z2 particle counter (Beckman Coulter). Procyclic forms (PCF ...
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bioRxiv - Cell Biology 2024Quote: ... The population density of the control and induced TbMyo21 RNAi cells was measured every 24 h over a time course of 96 h using a Z2 Coulter Counter (Beckman Coulter).
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bioRxiv - Developmental Biology 2023Quote: The anti–human uncoupled primary antibodies used are listed: anti-human CD34 (Beckman, QBEnd/10), anti-human ACE (BB9 ...
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bioRxiv - Biochemistry 2021Quote: ... Following centrifugation (Beckman SW60 rotor, 32,000 rpm, 18 h), the PSI-LHCI band was collected and used for subsequent experiments.
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bioRxiv - Immunology 2022Quote: ... anti-human TCR Vβ1 (Beckman Coulter), anti-human CD3 and CD8 antibodies (BD Bioscience).
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bioRxiv - Immunology 2022Quote: Human Vγ9Vδ2 T lymphocytes were stained with FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and APC-labeled anti-human CD3ε mAb (#UCHT1 ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was centrifuged at 10,500 rpm (∼20,000 × g, k factor = 1,753) for 70 min (SW28 rotor, Beckman). Pelleted lEVs were resuspended in 13.5 ml of PBS (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... and sedimented again at 10,500 rpm (∼20,000 × g, k factor = 1,983) for 70 min (SW40 rotor, Beckman). The supernatant containing sEVs was centrifuged at 25,000 rpm (∼ 110,000 × g ...
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bioRxiv - Cancer Biology 2024Quote: ... and the FOXP3/Transcription Factor Staining Kit (eBioscience) and run on the CytoFlex S cytometer (Beckman Coulter). Analysis was performed in FlowJo.
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bioRxiv - Microbiology 2022Quote: ... Lysates were then centrifuged for 1 h (25,000 rpm, Beckman Coulter Avanti J26-XP centrifuge ...
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bioRxiv - Biochemistry 2021Quote: ... Following centrifugation (Beckman SW40 rotor, 105,400xg, 18 h, 4°C), the heavier green band was collected and precipitated using 8% PEG6000 (Hampton Research ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was ultracentrifuged (Beckman 45Ti, 40,000 rpm, 1 h), and the supernatant was applied to an anti-FLAG M2 affinity gel (Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... After ultracentrifugation (Beckman Ti70 rotor, 150,000 × g for 1 h), the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [TN buffer + 1% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... for 16 h using ultra-clear tubes (Beckman Coulter, 344060). Twelve fractions of 1 mL each were collected manually from the top ...
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bioRxiv - Molecular Biology 2021Quote: ... according to the manufacturer’s instructions using a random nonamer tagged partial p7 sequence (CACGACGCTCTTCCGATCTNNNNNNNNN) and the first strand synthesis product was purified using AMPure XP beads (Beckman) following the manufacturer’s instructions with 1.8 volumes ...
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bioRxiv - Molecular Biology 2019Quote: ... Gradients were centrifuged 18-20h at 192,000g in a SW40 rotor (k-factor 144.5) (Beckman Coulter, Brea, CA). For RNA extraction and small RNA library preparation ...
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bioRxiv - Cell Biology 2021Quote: ... 100K EVs were isolated by ultracentrifugation of 10,000xg supernatant at 100,000xg for 65 min in a SW40 rotor (k-factor 280.3) (Beckman-Coulter). EV-containing 100K ultracentrifugation pellets were resuspended in 20 μL PBS containing 0.2% BSA (cleared from aggregates by ultracentrifugation for 16-20 hrs at 100,000xg) ...
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bioRxiv - Cancer Biology 2021Quote: ... 100,000xg for 3 h in an Optima XPN-80 (Beckman Coulter) ultracentrifuge using UltraClear Thinwall tubes ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 °C for 3 h (SW40 Ti rotor, Beckman-Coulter, Germany), the supernatant was discarded ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C for 1 h with MLS-55 rotor (Beckman Coulter). The pellets were lysed in 40 μL of radioimmunoprecipitation (RIPA ...
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bioRxiv - Genomics 2021Quote: ... following ultra-centrifugation 30,000g for 1 h (Rotor:Beckman Coulter, MLS-50) at 4°C ...
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bioRxiv - Genomics 2021Quote: ... following ultra-centrifugation 30,000xg for 1 h (Rotor:Beckman Coulter, MLS-50) at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... After ultracentrifugation (Beckman Ti70 rotor, 150,000 x g for 1 h), the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [HN buffer + 1% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C for 3 h using a SW41 rotor (Beckman Coulter). Sucrose gradients were fractionated using a Piston Gradient Fractionator (Biocomp) ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C for 3 h using a SW41 rotor (Beckman Coulter). Sucrose gradients were fractionated using a Piston Gradient Fractionator (Biocomp) ...