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Citations for Beckman :
1 - 50 of 547 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: CsCl linear gradients were prepared by overlaying 8.5 mL of 1.46 g/cm3 CsCl solution with 8.5 mL of 1.2 g/cm3 CsCl solution in 17 mL Ultra-Clear tubes (Beckman Coulter), which were then spun at a 45-degree angle and a speed of 20 rpm for 13.5 minutes using Gradient Master (BioComp).
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bioRxiv - Immunology 2023Quote: ... and soluble anti-CD28 (500 ng/ml; Beckman Coulter, REF# IM1376) antibodies in presence of cytokines i.e. ...
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bioRxiv - Microbiology 2019Quote: ... DNA from the labeled and unlabeled samples was added into separate quick seal 5ml tubes (Beckman Coulter, Indianapolis, IN, USA) combined with 1.88 g/ml CsCl and gradient buffer for a final buoyant density of 1.725 g/ml ...
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bioRxiv - Microbiology 2020Quote: ... DNA from the labeled and unlabeled samples was added into separate quick seal 5ml tubes (Beckman Coulter, Indianapolis, IN, USA) combined with 1.88 g/ml CsCl and gradient buffer for a final buoyant density of 1.725 g/ml ...
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bioRxiv - Immunology 2020Quote: ... After adding 2 mL of VersaLyse solution (Beckman Coulter, catalog number A09777) with 2.5% Fixative Solution (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... solution (0.2 µg/ml) and analyzed on a CytoFLEX S (Beckman Coulter) for DAPI-negative and dTomato-positive cells ...
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bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
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bioRxiv - Genomics 2020Quote: ... Then 10.5 ml solution was loaded into an ultracentrifuge tube (Beckman, Catalog 331374). Two mouse testes were freshly excised and immediately minced in a 35 mm petri dish containing 1 ml 0.9% NaCl ...
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bioRxiv - Biophysics 2021Quote: ... The density gradient was prepared by layering 4.5 mL of 30.4 % urografin solution on top of 4.5 mL of 53.2 % urografin solution in an ultra-clear centrifuge tube (Beckman Coulter; catalogue number 344059). The tubes were left at room temperature (25 °C ...
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bioRxiv - Plant Biology 2022Quote: ... was gently layered on top of 4.4 mL sucrose gradient solution (15 to 55%) in a 5.1 mL Quick-Seal polypropylene tube (Beckman Coulter) and centrifuged at 45,000 rpm for 65 min at 4°C (Sorval NVT 90 ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
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bioRxiv - Immunology 2023Quote: ... Layers of 2.1 mL of each solution were successively poured into 12.5 mL polyallomer tubes (Beckman-Coulter, Cat. # 331372), starting from the most concentrated solution (50 % ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was formed by adding 3 mL of the iodixanol solutions to a 17 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mL samples were supplemented with 500 uL of Optiprep to yield a 20% iodixanol solution and underlayered beneath 1.5 mL layers of 9% and 12% Optiprep/PBS solutions in a 4.9 mL OptiSeal tube (Beckman-Coulter, 362185) using a disposable glass cannula ...
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bioRxiv - Cell Biology 2022Quote: ... The solution was transferred to a 0.8 mL Ultra-Clear centrifuge tube (Beckman Coulter) and overlaid with 250 µL of Nycodenz 30% in buffer H and 75 µL of buffer H ...
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bioRxiv - Biochemistry 2020Quote: ... The labeled and unlabeled polymerized actin samples were pelleted down by ultracentrifugation at room temperature (45000 rpm for 1 hour, Beckman TLA-55 rotor). The pellets were rinsed once with 1 mL G-buffer and re-suspended into ice cold 0.5 mL G-buffer ...
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bioRxiv - Microbiology 2024Quote: ... Four mL cultures at ∼10% parasitemia were harvested by centrifugation (2000 rpm for 3 min in a Beckman Coulter Allegra 25R table-top centrifuge ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...
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bioRxiv - Neuroscience 2022Quote: ... and 50% sucrose solutions (1.5 ml each) were layered into a centrifuge tube (Beckman, 331372). Brain total lysate (6 mg of protein ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...
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bioRxiv - Neuroscience 2023Quote: ... The nuclei suspension was layered onto 10 mL of 29% OptiPrep solution (Buffer + 50% OptiPrep) in a 38.5 mL Ultra-Clear tube (Beckman-Coulter, CA, USA) before being centrifuged at 7,333g for 30 minutes at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 mL of 5% solution in a 14 x 89 mm (13.2 mL) thin walled ultra-clear centrifuge tubes (Beckman Coulter, Cat. # 344059). Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting lysate was loaded on top of 10 ml 29% iso-osmolar OptiPrep solution in a conical 30 ml centrifuge tube (Beckman Coulter, SW28 rotor). Samples were centrifuged at 10,100 × g (7,500 r.p.m. ...
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bioRxiv - Molecular Biology 2019Quote: ... The concentrated urine sample was resuspended in 3.2 ml of a 50% iodixanol solution and layered on the bottom of a 17 ml Thinwall Polypropylene Tube (Beckman Coulter, Fullerton, California, USA). A discontinuous DG was prepared by additional layering of 4 ml of 20% ...
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bioRxiv - Bioengineering 2023Quote: ... by adding 10 μL of the PBMC solution to 10 mL of Isoton II diluent (Beckman Coulter) in a 20 mL cuvette ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 ml of each solution were used to form the gradient in a 14 ml Ultra-Clear™ centrifuge tube (Beckman Coulter, Brea, CA, USA), with the highest sucrose concentration at the bottom of the tube ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells mixed with polybrene and lentivirus were then plated in 1 mL aliquots onto 12 well plates and centrifuged at 2000 rpm (931 x g) [Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A] for 2 hours at 30°C ...
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bioRxiv - Systems Biology 2020Quote: ... then resuspended the pellet in 0.1% BSA-PBS containing 100 ng/mL DAPI and proceeded with FACS on a MoFlo Astrios (Beckman Coulter). After gating for singlets and live cells ...
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bioRxiv - Immunology 2022Quote: ... Every 4 days cells were fed by replacing 25 μl of media with fresh I10 containing irradiated 2.5 × 103 3T3-msCD40L cells and 50 ng/mL IL-21 using a Biomek NXP liquid handler (Beckman Coulter). Cells were cultured for 2-3 weeks before harvesting the final supernatant and either freezing the cultures in the 384-well plate (50ul freezing media per well (FBS + 10% DMSO)) ...
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bioRxiv - Microbiology 2022Quote: ... solution was added 1:1 to EV suspensions and transferred to an MLS-50 rotor tube (Beckman; 344057). Iodixanol stock was then diluted in 0.25 M sucrose-Tris buffer to make 10% and 20% iodixanol solutions ...
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bioRxiv - Plant Biology 2020Quote: ... A Biomek 2000 workstation (Beckman Coulter, CA, USA) was used to aliquot the qRT-PCR master mix into a 384-well plate ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 % solutions on top of each other in a 13.2 ml open-top polyallomer tube (331372, Beckman Coulter). The 1 ml concentrates were overlaid on top of the gradient ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 mL of 30% and 1.8 mL 10% of iodixanol solutions and floated into the gradient by ultracentrifugation in a SW60Ti rotor (Beckman) at 192,000 x g ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 5.1 ml of the solution was transferred to an ultracentrifuge tube (Beckman Coulter QuickSeal, 13 mm × 51 mm) and heat-sealed ...
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bioRxiv - Microbiology 2023Quote: ... and 5.5 ml was layered on top of a two-step sucrose gradient consisting of 5 ml of 40% sucrose solution layered on top of 1.5 ml of a 65% sucrose solution in a 14 x 89mm Ultra-Clear tube (Beckman Coulter). All sucrose (w/w ...
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bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
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bioRxiv - Cell Biology 2022Quote: ... which was then slowly poured on top of 10 ml cushion solution (2.05M sucrose with protease inhibitors, DTT and PMSF) in the ultracentrifugation tube (Beckman polyallomer #326823). After a 45-min spin at 24,600 rpm in pre-chilled SW32Ti rotor at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Plant Biology 2023Quote: ... The combined cAW and vAW solutions were then centrifuged using 29 x 104 mm (50 mL) polypropylene centrifuge tubes (Beckman Coulter, # 357007) at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Biochemistry 2019Quote: ... Plates were centrifuged at 2000 rpm (Allegra X-15R - Beckman Coulter, Inc.) for 2 min followed by 2 additional 10% TCA washes and one ethanol wash followed by centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... CD81-APC (Beckman Coulter, clone JS64, A87789; 0.6 ng/µl), H3-AF488 (Invitrogen ...
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bioRxiv - Systems Biology 2021Quote: ... E2T between 101 and 102 using Latin Hypercube Sampling (McKay, Beckman, & Conover, 2000) for 20000 different sets ...
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bioRxiv - Pathology 2020Quote: ... 50 μl of Truecount bead solution (Beckman Coulter) was added to each sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Illumina RNASeq sequencing (HiSeq 2000, 2 × 75 pb) and trimming were performed by Beckman Coulter Genomics ...
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bioRxiv - Immunology 2020Quote: ... resuspended in VersaLyse solution (Beckman Coulter, catalog number A09777) with 2.5% Fixative Solution (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... with 2.5% Fixative Solution (Beckman Coulter, catalog number A07800) and incubated 15 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... with 2.5% Fixative Solution (Beckman Coulter, catalog number A07800), samples were further incubated for 15 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Recordings of children speaking were from the CHILDES database (MacWhinney, 2000; Edwards and Beckman, 2008) from TalkBank (talkbank.org) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were harvested after 72 h by centrifugation at 2000 rpm (JLA8.1 rotor, Beckman-Coulter), washed with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... pelleted SVF was resuspended in VersaLyse solution (Beckman Coulter #A09777) according to the manufacturer’s recommendations and washed once with 1% albumin solution ...