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Citations for Beckman :
1 - 50 of 1068 citations for Norovirus GII.4 VLP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The VLPs were concentrated by ultracentrifugation for 2 h at 31,000 rpm at 4 °C (Beckman SW-31Ti rotor), and then resuspended in 500 μL of Ex-Cell 405 medium ...
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bioRxiv - Microbiology 2022Quote: ... Pseudotypes and VLPs were then concentrated by ultracentrifugation (20,000 RPM, 4 ºC, 1.5h, Beckman Coulter Optima XPN-100, SW32Ti rotor) through a 20% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... the VLPs were pelleted for 2 h at 40,000 rpm (Beckman TLA55) at 4°C and subsequently resuspended in PBS (pH 7.4) ...
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bioRxiv - Biophysics 2020Quote: ... VLPs were pelleted for 2 h at 40,000 rpm (Beckman TLA55 rotor) at 4°C and solved in PBS (pH 7.4).
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bioRxiv - Molecular Biology 2019Quote: ... The VLPs in the supernatant were concentrated by ultracentrifugation at 35,000 rpm (Beckman Ti45) for 2 h at 4°C and then further purified using CsCl equilibrium gradient ultracentrifugation at 35,000 rpm (Beckman SW56 ...
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bioRxiv - Biophysics 2020Quote: ... VLPs in the supernatant were concentrated by ultracentrifugation at 35,000 rpm (Beckman Ti45 rotor) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... the VLPs were concentrated by ultracentrifugation (40,000 rpm, 2h, 10°C, Beckman SW41 rotor), resuspended in buffer A and observed by TEM ...
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bioRxiv - Biophysics 2020Quote: ... VLPs were further purified using CsCl equilibrium gradient ultracentrifugation at 35,000 rpm (Beckman SW56 rotor) for 18 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... VLPs were concentrated using ultracentrifuge (TLA100.3 rotor, Beckman; at 72,000 rpm for 1 hour, +5°C) or by ultrafiltration using Amicon Ultra 15 (100 kDa ...
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bioRxiv - Biophysics 2021Quote: ... 24h after transfection cells media (9mL) were filtered before performing VLPs purification by ultracentrifugation (SW41Ti rotor (Beckman) 29 000rpm ...
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bioRxiv - Immunology 2020Quote: CCMV-SS and CCMV-Ctt830-SS VLPs were separated from cellular proteins by ultracentrifugation (SW28 rotor, Beckman, Palo Alto ...
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bioRxiv - Microbiology 2021Quote: ... VLPs were concentrated by ultracentrifugation through a 20% (w/w) sucrose cushion using a Beckman SW32Ti rotor (Beckman-Coulter) at 20,200 rpm for 2h at 4°C.
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml) and cell subsets were discriminated using the following antibody panel: CD45 (Clone J33; Beckman Coulter, Brea, CA), CD11c (Clone BU15 ...
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bioRxiv - Biochemistry 2022Quote: ... VLPs were concentrated over 500 μL of 20% sucrose in PBS at 50,000 x g for 45 min (Beckman Coulter Optima TLX). Infections were assessed by western blot using the following antibodies ...
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bioRxiv - Genetics 2023Quote: ... in-house VLPs were produced using the same protocol followed by ultracentrifugation at 24,000 RPM for 2 hours (SW 28 Beckman swinging bucket rotor) to concentrate the VLPs and avoid diluting the HSPCs culture media ...
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bioRxiv - Molecular Biology 2022Quote: ... 4°C (Beckman Coulter). The pellet containing lentiviral particles was suspended in the residual medium ...
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bioRxiv - Genomics 2020Quote: ... 4°C (Beckman Coulter). Supernatant was loaded onto 5 ml HisTrap FF columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C (Beckman Coulter). Clarified lysates were poured several times over 0.5-1 ml Streptactin resin (IBA ...
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bioRxiv - Cancer Biology 2023Quote: ... 4°C (Beckman Coulter). The pellet containing lentiviral particles was suspended in the residual medium ...
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bioRxiv - Biochemistry 2019Quote: ... 4 °C (Beckman JLA 16.2500) and then bound to 0.1 cell paste volumes of anti-FLAG resin (Sigma M2 ...
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bioRxiv - Biochemistry 2019Quote: ... 4 °C (Beckman JLA 8.1000), washed in pre-chilled PBS and resuspended in 20 cell paste volumes of preparation buffer (50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... A Multisizer 4 (Beckman Coulter) machine was used ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (SW55Ti rotor, Beckman). The pellets were resuspended in 25% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (VTi65.1 rotor, Beckman) with the maximum acceleration and minimum deceleration allowing for isopycnic separation of subcellular particles and membranes ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C (SW41 rotor, Beckman). The supernatant was concentrated with a 100-kDa molecular weight cutoff concentrator (Merck Millipore ...
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bioRxiv - Microbiology 2019Quote: ... performed ultracentrifugation (40,000 rpm, either 4 h or 18 h, +4 °C, SW41 rotor, Beckman Coulter), and collected approximately 600 μl fractions by puncturing the tubes with a thin (23G or 25G ...
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bioRxiv - Cancer Biology 2021Quote: ... 4°C (Beckman Coulter Allegra X15r). After the final wash to remove Matrigel ...
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bioRxiv - Molecular Biology 2021Quote: ... 4°C (SW41Ti rotor, Beckman Coulter) and 16 fractions were collected ...
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bioRxiv - Molecular Biology 2021Quote: ... 4°C (SS34 Rotor, Beckman Coulter).
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bioRxiv - Cell Biology 2020Quote: ... 4°C (with Beckman SW32 rotor). The pellet will contain rough microsomes and the interface between 0.25-1.3M will contain smooth microsomes ...
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bioRxiv - Neuroscience 2021Quote: ... 4°C (Beckman Coulter F301.5 rotor) to remove large aggregates ...
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bioRxiv - Biophysics 2023Quote: ... 4 °C (TLA-45 Beckman rotor). The solubilized proteins were separated on 4–15 % BN-gels and analyzed by immune detection using polyclonal α-YidC antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... KG, Staufen, Germany) (position 6, 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Microbiology 2020Quote: ... 4°C in an SW55Ti rotor (Beckman), and the virus pellet harvested for analysis by WB with αGag.
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Cell Biology 2020Quote: ... 4 °C with 70Ti rotor (Beckman Coulter) to collect sEV pellets in double 0.22 μm-filtered PBS ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C in SW41 rotor (Beckman Coulter). Samples were fractionated and collected using Biocomp Piston Gradient Fractionator (BioComp Instruments) ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C in SW28 rotor (Beckman Coulter). Gradients were fractionated using Biocomp Piston Gradient Fractionator (BioComp Instruments) ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C in SW41 rotor (Beckman Coulter). Polysomes profiles were obtained using Biocomp Piston Gradient Fractionator (BioComp Instruments ...
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bioRxiv - Microbiology 2024Quote: ... 4°C (Optima TLX Ultracentrifuge, Beckman Coulter). The pellet was resuspended in 200 µL of ultrapure water.
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bioRxiv - Cell Biology 2021Quote: ... then finally at 100,000 g for 4 h at 4 ℃ using an SW28 rotor (Beckman Coulter, Fullerton, CA, USA). The pelleted exosomes were collected then subsequently resuspended in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Hettich) at 4°C and then centrifuged at 40000 rpm for 4h30 at 4°C using SW41Ti rotor (Beckman). The supernatant was then UV inactivated at a wavelength of 254 nm for 4 min (3 times ...
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bioRxiv - Microbiology 2023Quote: ... The lysates containing matured PsVs were loaded on an OptiPrep gradient that had been stabilized at least 1 h and centrifuged at 50k x g for 3.5-4 h at 4°C in a SW-55Ti rotor (Beckman). Fractions were collected in siliconized tubes and subjected to SDS-PAGE followed by Coomassie blue staining to assess the abundance of L1 and L2 proteins ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were spun at 200,000 × g at 4°C for 4 h in an MLS-50 rotor (Beckman Coulter), and 60 µL of top layer was collected ...
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bioRxiv - Biophysics 2019Quote: ... Total volume was centrifuged at 10000 xg for 30 minutes at 4°C at 10000 xg for 30 minutes at 4 °C (Beckman Coulter Optima L-80 XP Ultracentrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... which were centrifuged at 50,000rpm for 4 hours at 4°C using a TLS55 rotor and an Optima MAX-XP Ultracentrifuge (Beckman Coulter). The sample was physically fractionated into 10 fractions of 85 uL each ...
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bioRxiv - Molecular Biology 2022Quote: ... 800 µl of EDTA-treated lysate was layered on top of the sucrose gradient and ultracentrifuged at 35000 rpm for 4 hours at 4 °C in a SW41Ti rotor (Beckman). After centrifugation ...
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bioRxiv - Immunology 2020Quote: ... all CCMV CP preparations were purified two times using an ultracentrifuge and 30% sucrose cushion – first with additional 0.5% Triton X-100 and the second time without Triton X-100 (4 h, 50 000 rmp, 4°C; Type 70Ti rotor, Beckman) and the pellet was then dissolved in buffer A ...