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Citations for Beckman :
1 - 50 of 234 citations for Mouse Anti Chikungunya Virus VLP D7 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... the VLPs were pelleted for 2 h at 40,000 rpm (Beckman TLA55) at 4°C and subsequently resuspended in PBS (pH 7.4) ...
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bioRxiv - Biophysics 2020Quote: ... VLPs were pelleted for 2 h at 40,000 rpm (Beckman TLA55 rotor) at 4°C and solved in PBS (pH 7.4).
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bioRxiv - Molecular Biology 2019Quote: ... The VLPs in the supernatant were concentrated by ultracentrifugation at 35,000 rpm (Beckman Ti45) for 2 h at 4°C and then further purified using CsCl equilibrium gradient ultracentrifugation at 35,000 rpm (Beckman SW56 ...
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bioRxiv - Biophysics 2020Quote: ... VLPs in the supernatant were concentrated by ultracentrifugation at 35,000 rpm (Beckman Ti45 rotor) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... the VLPs were concentrated by ultracentrifugation (40,000 rpm, 2h, 10°C, Beckman SW41 rotor), resuspended in buffer A and observed by TEM ...
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bioRxiv - Biophysics 2020Quote: ... VLPs were further purified using CsCl equilibrium gradient ultracentrifugation at 35,000 rpm (Beckman SW56 rotor) for 18 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... VLPs were concentrated using ultracentrifuge (TLA100.3 rotor, Beckman; at 72,000 rpm for 1 hour, +5°C) or by ultrafiltration using Amicon Ultra 15 (100 kDa ...
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bioRxiv - Biophysics 2021Quote: ... 24h after transfection cells media (9mL) were filtered before performing VLPs purification by ultracentrifugation (SW41Ti rotor (Beckman) 29 000rpm ...
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bioRxiv - Immunology 2020Quote: CCMV-SS and CCMV-Ctt830-SS VLPs were separated from cellular proteins by ultracentrifugation (SW28 rotor, Beckman, Palo Alto ...
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bioRxiv - Microbiology 2021Quote: ... VLPs were concentrated by ultracentrifugation through a 20% (w/w) sucrose cushion using a Beckman SW32Ti rotor (Beckman-Coulter) at 20,200 rpm for 2h at 4°C.
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bioRxiv - Microbiology 2021Quote: ... The VLPs were concentrated by ultracentrifugation for 2 h at 31,000 rpm at 4 °C (Beckman SW-31Ti rotor), and then resuspended in 500 μL of Ex-Cell 405 medium ...
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bioRxiv - Microbiology 2022Quote: ... Pseudotypes and VLPs were then concentrated by ultracentrifugation (20,000 RPM, 4 ºC, 1.5h, Beckman Coulter Optima XPN-100, SW32Ti rotor) through a 20% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml) and cell subsets were discriminated using the following antibody panel: CD45 (Clone J33; Beckman Coulter, Brea, CA), CD11c (Clone BU15 ...
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bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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bioRxiv - Biochemistry 2022Quote: ... VLPs were concentrated over 500 μL of 20% sucrose in PBS at 50,000 x g for 45 min (Beckman Coulter Optima TLX). Infections were assessed by western blot using the following antibodies ...
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bioRxiv - Genetics 2023Quote: ... in-house VLPs were produced using the same protocol followed by ultracentrifugation at 24,000 RPM for 2 hours (SW 28 Beckman swinging bucket rotor) to concentrate the VLPs and avoid diluting the HSPCs culture media ...
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bioRxiv - Systems Biology 2020Quote: ... tetramethylrhodamine (TRITC)-conjugated anti-mouse IgM (#731781, Beckman Coulter), and Alexa-594-conjugated anti-rabbit IgG (A11012 ...
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bioRxiv - Microbiology 2024Quote: ... The virus was enriched by ultracentrifugation (Beckman Coulter Optima™ L-80XP) at 246,347g for 4 hours at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... secondary anti-mouse-HRP (Beckman Coulter, Ref. 715-035-151), secondary anti-rabbit-HRP (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... mouse anti-CD79b PE (clone CB3-1) (IM1612U) was from Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... mouse-anti-CD159a-PE (Beckman Coulter, catalog #IM3291U, dil 1:30), and mouse-anti-CD14-BV650 (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2020Quote: Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 28) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor) for 2h at 4 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... including PE-Cy7-conjugated mouse anti-human CD45 (Beckman Coulter, CA, USA), efluor660-conjugated rat anti-human OCT3/4 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
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bioRxiv - Biochemistry 2021Quote: ... The virus-containing filtrate was then further purified using an ultracentrifuge (Optima XPN-90 Ultracentrifuge, Beckman Coulter, USA) with a cycle of 1,000 rpm (116 g ...
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bioRxiv - Immunology 2019Quote: The following antibodies were used: anti-p24-FITC (mouse monoclonal, Beckman and Coulter, #KC-57); anti-CD3-Pacific Blue (mouse monoclonal ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with primary anti-HIV-Gag antibody (Beckman-Coulter; KC57-FITC, mouse derived), and cells were acquired using the MACs Quant Flow Cytometer (Milentyi ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with primary anti-HIV-Gag antibody (Beckman-Coulter; KC57-RD1, mouse derived), and with α-mouse Alexa Fluor555-conjugated secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The virus was then purified over a 20% sucrose cushion by centrifugation at 35,000 rpm (Beckman Coulter SW41 rotor) for 3 h at 4°C ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Molecular Biology 2020Quote: ... Virus particles in the supernatant were pelleted by ultracentrifugation at 45.000 rpm for 45 min (Beckman 70.1 Ti Rotor) over a pad of 15% sucrose in 10 mM Tris-HCl buffer (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... Virus RNA was extracted with a magnetic bead-based RNA extraction kit (RNAdvance Viral Reagent kit, Beckman Coulter, C63510) on an epMotion® 5073t liquid handler (Eppendorf ...
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bioRxiv - Cell Biology 2021Quote: ... platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin ...
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bioRxiv - Cell Biology 2021Quote: Platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Incubation was performed at room temperature for 10 minutes in the dark ...
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bioRxiv - Microbiology 2021Quote: ... and 10μl of PE-conjugated mouse-anti-human langerin Ab (Clone DCGM4; Beckman Coulter, Marseille, France). In other experiments ...
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bioRxiv - Microbiology 2019Quote: ... A portion of each sample was stained with Mouse-Anti Human CD15-PE (Beckman Coulter, USA) and run under BD Accuri C6 Flow Cytometer ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
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bioRxiv - Biophysics 2023Quote: ... Virus-containing supernatants were collected at 48 hours and 72 hours after transfection and concentrated using ultracentrifugation (Ultraclear, Beckman 344058). The transduction capacity of the viral vector product was confirmed by culturing non-transfected HEK293T cells with the virus after 24 hours and assessing via fluorescent imaging.
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter; 1:100 dilution). The percentage of transfected or infected cells (p24+ ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and virus pelleted through 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) in combination with Nef or Vpu rabbit antisera (1:1000 dilution) ...
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bioRxiv - Immunology 2021Quote: ... as assessed by flow cytometry with Korman orange-conjugated mouse anti-human CD19 monoclonal antibody (mAb) (Beckman Coulter, CA, USA). The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza ...
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bioRxiv - Microbiology 2023Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA, 1:100 dilution). Flow cytometry measurements were performed using a FACSCalibur (BD Biosciences ...
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bioRxiv - Pathology 2023Quote: ... the proportion of HIV-1-infected cells was determined by intracellular staining of the viral protein Gag-p24 with a Mouse anti-p24-FITC antibody (clone KC57, Beckman Coulter).
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bioRxiv - Immunology 2024Quote: ... The sections were washed and incubated for two hours with anti-human mouse CD14 Texas Red (clone: RMO52) (Beckman Coulter, USA). Afterward ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal antibodies recognizing the closed conformation of β1 integrin (unoccupied β1 integrin, termed ‘inactive’) were mouse anti-human β1 integrin 4B4 (Beckman Coulter, 6603113) and rat anti-human β1 integrin mAb13 (generated in house from a hybridoma) ...