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Citations for Beckman :
1 - 50 of 767 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The mixtures were loaded into ultracentrifuge tubes (7 x 20 mm, Beckman Coulter) and fractionated at 100,000Xg using a Beckman TL-100 ultracentrifuge ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound protein was separated from liposomes by transferring 100μl of this mixture to 7×20mm PC ultracentrifuge tubes (Beckman) and carefully layering an equal volume of 0.75M sucrose buffer followed by 25μl of sucrose-free HK buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was clarified by centrifugation at 90,000 rpm (TLA-100; Beckman Coulter) for 5 minutes at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... This mixture was then spun down in a TLA-100 rotor (Beckman Coulter) at 9,892 g for 30 min at 4°C to sediment actin filament bundles into a low-speed pellet ...
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bioRxiv - Cell Biology 2020Quote: ... and layered onto a continuous 10-50 w/w % sucrose gradient prepared in a 2.2 ml ultra-clear tube (11×34 mm, Beckman Coulter) using a two-step program in Gradient Master 108 machine ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... 1.6 ml of 35% (w/v) sucrose and 5% (w/v) sucrose in MBS were layered in a 5.1 ml ultracentrifuge tube (Beckman Coulter, #326819) to form a discontinuous sucrose gradient ...
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bioRxiv - Systems Biology 2020Quote: ... then resuspended the pellet in 0.1% BSA-PBS containing 100 ng/mL DAPI and proceeded with FACS on a MoFlo Astrios (Beckman Coulter). After gating for singlets and live cells ...
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bioRxiv - Genetics 2020Quote: ... 99 µl Agencourt RNAclean XP (Beckman Coulter Life Sciences) were added and incubated at RT for 15 min ...
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bioRxiv - Genetics 2022Quote: ... with purity > 99% using a MoFlo XDP instrument (Beckman Coulter) (Table S1 ...
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bioRxiv - Microbiology 2024Quote: ... 50% (w/v) sucrose) was filled into ultracentrifuge tubes (Polypropylene 10 ml Tube, Beckman Coulter), the sheared grappling hooks were gently layered on top ...
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bioRxiv - Bioengineering 2021Quote: ... or 7-Amino-Actinomycin D (7-AAD, Beckman Coulter, B88526) was added to the cell suspensions ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was incubated on ice for 10 minutes then clarified at 90,000 rpm (TLA-100; Beckman Coulter), 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ml of each lysate was mixed with 2 ml of 1% sarkosyl and then layered on 2 ml of 150% (w/v) cesium chloride (in 1X TE, pH 7.5) in 4.9 ml OptiSeal tubes (Beckman Coulter, cat #362185). Samples were centrifuged for 18 h at 42,000 rpm in an NVT 65.2 rotor (Beckman Coulter ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant (33 mL) was placed on a 3 mL 25% (w/v) sucrose pad in SW28 tube (Beckman, Brea, CA, USA) and centrifuged at 24,000 rpm ...
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bioRxiv - Immunology 2023Quote: ... and soluble anti-CD28 (500 ng/ml; Beckman Coulter, REF# IM1376) antibodies in presence of cytokines i.e. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...
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bioRxiv - Biochemistry 2022Quote: ... Reaction mixtures were incubated for 20 minutes at 25 °C then centrifuged at 60,000 rpm (TLA-100; Beckman Coulter) for 15 minutes at 25 °C ...
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bioRxiv - Systems Biology 2019Quote: ... then resuspended in 1% BSA-PBS containing 100ng/mL propidium iodide or 200ng/mL 7-AAD and proceeded with sorting using a MoFlo Astrios (Beckman Coulter) or FACSJazz (BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... Particles were then concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion at 28,000 rpm using a Beckman TLA-100 fixed angle-rotor (Beckman Coulter) for 90 min or for the 6-well format at 44,000 rpm using a Beckman TLA-55 fixed angle-rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA mixture was then purified using a mixture of AMPure XP beads (Beckman Coulter) with 40% polyethylene glycol (mixed in a 7:3 ratio) ...
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bioRxiv - Molecular Biology 2020Quote: ... The assembled complexes were incubated for 5 min at 30 °C and for 10 min on ice before they were loaded on a 30% (w/v) sucrose cushion in TLA-100 tubes (Beckman Coulter). The sucrose cushions were centrifuged for 2 h at 390,880 xg and 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 100 ng of DNA from each sample was buffer exchanged via Ampure XP bead purification (Beckman Coulter Life Sciences product number A63881 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mL of 20% (w/v) sucrose in DPBS (-Ca, -Mg) is prepared in ultracentrifuge tubes (Beckman Coulter, 344058) to which the divided EnvA- pseudotyped viral media is added before pelleting (20,000 RPM for 2 hours at 4°C) ...
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bioRxiv - Microbiology 2019Quote: ... The entire 3 ml mixture was placed at the bottom of a Beckman SW41 ultracentrifuge tube (Beckman, Munich, Germany) and subsequently overlaid with 5 ml of ice-cold 30% Optiprep in TNE and 4 ml of ice-cold 5% Optiprep in TNE ...
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bioRxiv - Plant Biology 2021Quote: ... 100 μg/mL cycloheximide in 60% sucrose) in a 26 ml polycarbonate tube (Beckman Coulter). After proper balancing ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
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bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
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bioRxiv - Neuroscience 2023Quote: ... Fractions were washed by diluting with 7 mL of PBS and ultracentrifuged at 100,000 x g (Type 70.1 Ti rotor, Beckman Coulter) for 2 h ...
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bioRxiv - Microbiology 2021Quote: ... VLPs were concentrated by ultracentrifugation through a 20% (w/w) sucrose cushion using a Beckman SW32Ti rotor (Beckman-Coulter) at 20,200 rpm for 2h at 4°C.
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bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
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bioRxiv - Biophysics 2021Quote: ... The proteoliposomes were mixed with 60% (w/v) sucrose and loaded at the bottom of a 10 ml polycarbonate centrifuge tube (Beckman Coulter). A discontinuous sucrose gradient was prepared (20% ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were solubilised in 150 μL of cold TNE buffer and a volume of 1.5 ml was laid on a five step 20 - 60% (w/v) sucrose gradient prepared in Ultra-Clear 13 ml tubes (Beckman Coulter). The tubes were centrifuged at 35,000 rpm for 2h at 4°C in a Beckman SW41 rotor ...
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bioRxiv - Biophysics 2021Quote: ... solubilized supernatant was collected and subjected to sucrose gradient ultracentrifugation (10% to 50% (w/v) sucrose in buffer A with 0.03% (w/v) digitonin) at 35000 rpm (sw41 Ti rotor, Beckman Coulter) for 16 hours at 4℃ ...
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bioRxiv - Microbiology 2020Quote: ... OBs were loaded on to a discontinuous 35–60% (w/w) sucrose gradient and centrifuged at 20000 rpm and 4°C for 1 hour in a SW41 rotor (Beckman). The band containing the OBs was removed ...
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bioRxiv - Physiology 2019Quote: ... MBS was added to the homogenate until a volume of 2.5 mL was reached then mixed with 2.5 mL of 90% sucrose in MBS and 4mL of this mixture was added to an ultracentrifuge tube (Beckman Coulter 344061). A sucrose gradient was generated by adding 4 mL 35% sucrose followed by 4mL of 5% sucrose ...
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bioRxiv - Biochemistry 2022Quote: ... by dissolving the yeast pellet in 100 mL wash buffer per 100 g cell pellet and centrifugation (JLA 8.1000 rotor, Beckman) at 5,000 rpm ...
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bioRxiv - Microbiology 2021Quote: ... The remaining lysate was layered on top of a 30% (w/w) sucrose cushion and centrifuged using a Beckman SW41 rotor (Beckman Coulter) at 32,000rpm and 4°C for 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... was loaded onto 4 ml of a 10% −30% (w/v) linear sucrose gradient and centrifuged at 74,000g at 4°C for 6 h (Beckman MLS-50 rotor). Fractions (250 µl ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (18) ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (16) ...
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bioRxiv - Microbiology 2024Quote: ... Liposomes and liposome-protein complexes were separated from unbound protein by ultracentrifugation of the reaction mixture in 5 mL Optiprep™ (Axis-Shield PoC AS) density gradients for 1 hour at 40,000 ×g (SW55Ti Beckman Coulter rotor). The liposome-protein mixture was adjusted to 34% Optiprep concentration in a volume of 300 μl ...
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bioRxiv - Genomics 2023Quote: ... Five million cells were harvested at 315xg for 7 minutes in a 15 mL centrifuge tube (Beckman Coulter Allegra X-14R) and stored at -80°C ...
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bioRxiv - Microbiology 2021Quote: ... unbound compound was separated from the astodrimer:virus mixture by pelleting the preincubated mixture through a 20% sucrose cushion (Beckman SW40 Ti rotor). The astodrimer sodium-containing supernatant was removed (i.e. ...
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bioRxiv - Immunology 2020Quote: ... and antibodies against membrane markers were added (CD45, CD14, HLA-DR from Beckman Coulter). Intracellular staining was performed using the IntraPrep fixation permeabilization kit (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... 0.2% Triton X-100 and 100 µg/mL chloramphenicol) and ultracentrifuged at 42500 rpm for 5 h in type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 100 ng of DNA was sheared to 300 bp using the Covaris LE220 and size selected using SPRI beads (Beckman Coulter). The fragments were treated with end-repair ...
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bioRxiv - Microbiology 2022Quote: ... 100 µg/mL cycloheximide) in a Beckman 14×89mm tube (Beckman Coulter 331372) and adding 5.5 mL of 15% sucrose solution (42.5 mL 1X LSB (20 mM Tris pH 7.4-7.5 ...
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bioRxiv - Pathology 2022Quote: Cell death and apoptotic markers were recorded by flow cytometry (FC500, Beckman Coulter, Villepinte, France). Cell viability was analyzed by FDA (Fluorescein di-acetate ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1% DMSO) by pin tool to 30 µL of the reaction mixture A (buffer assay mixture and substrate) with shaking (Beckman Biomek FXP, Brea, CA). The activity assay was started by adding reaction mixture B (enzyme in storage buffer ...