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Citations for Beckman :
1 - 42 of 42 citations for Legionella pneumophila antigen native extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... extracts were ultracentrifuged (Beckman Coulter Optima) at 100 000 rpm for two hours ...
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bioRxiv - Immunology 2022Quote: ... HIV-1 p24 core antigen KC57: 6604667 from Beckman Coulter and APC HIV-1 (p24 ...
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bioRxiv - Microbiology 2023Quote: ... and HIV-1 core antigen-FITC (KC57, Beckman Coulter; 6604665).
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bioRxiv - Molecular Biology 2019Quote: The native ChIP DNA (fragments longer than 100bp) was isolated using 1x SPRI beads (Beckman Coulter) and set up in an overnight in vitro transcription reaction (HiScribe T7 Quick High Yield RNA Synthesis kit ...
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bioRxiv - Immunology 2023Quote: ... Surface antigen expression was measured with the CytoFLEX LX (Beckman Coulter) and data was analyzed with the CytExpert Software Version 2.4 (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... staining with anti-HIV-1 Core Antigen Clone KC57 FITC (Beckman Coulter) and detection of HIV-1-infected cells by flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... Cells were probed for HIV-1 core antigen-FITC KC57 (Beckman Coulter) for 1 hr at 4°C then washed with PBS and resuspended in FACS buffer (5% FBS in PBS) ...
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bioRxiv - Microbiology 2020Quote: ... and p24 antibodies (anti-HIV core antigen: clone KC57, Beckman Coulter, 1:200).
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bioRxiv - Cancer Biology 2022Quote: ... Antigen cross-presentation was assessed using a Cytoflex S flow cytometer (Beckman Coulter) and was measured as the percentage of APC+ cells within CD45highCD11b+ MdCs and CD45lowCD11b+ MG ...
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bioRxiv - Molecular Biology 2024Quote: ... The extract was centrifuged in an ultracentrifuge (Beckman, SW60 rotor) at 4 °C for 30 min and the supernatant transferred to a fresh tube ...
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bioRxiv - Microbiology 2020Quote: ... The dyes used were: KC57 anti-HIV-1 core antigen-PE (Beckman coulter CO604667), Live/Dead fixable Far Red Dead Cell Stain Kit (Invitrogen L10120 ...
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bioRxiv - Microbiology 2021Quote: ... PE) and HIV-1 Gag core antigen (FH190-1-1, Beckman Coulter; PE, FITC). All samples were acquired on either an BD Fortessa X20 or LSR II using BD FACSDiva software and analyzed using FlowJo v10 (Tree Star) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were tagged using barcodes NB01 to NB05 of the Native Barcoding Expansion 1-12 PCR-free kit (EXP-NBD104, ONT) and purified using Agencourt AMPure XP beads (Beckman Coulter). The barcoded samples were quantified using a Qubit fluorometer and pooled equimolarly to reach 700 ng in 65 μL ...
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bioRxiv - Genomics 2023Quote: ... we extract genomic DNA from earpunch tissue using the Agencourt DNAdvance Kit (Beckman Coulter Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... The cell extract was centrifuged at 20,000 rpm in a JA 25.50 (Beckman) rotor for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... The extracts were cleared by centrifugation at 8000 rpm (JA25.50 rotor, Beckman-Coulter) for 45 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... and the crude extract was centrifuged at 39,000 xg (JA-17 rotor, Beckman-Coulter) for 30 min at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... and the crude extract was centrifuged at 17000 g (JA-17 rotor, Beckman-Coulter) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... was added to the RNA extracts that were subsequently handled and analysed by Beckman Coulter Genomics according to the standard Affymetrix GeneChip protocol for bacteria ...
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bioRxiv - Genetics 2019Quote: ... A clear extract obtained after centrifugation at 35,000 rpm for 30 min (Beckman 50.2Ti rotor) was loaded onto a HiPrep 16/10 Heparin FF column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volume (2 ml i.e 1600 µg) of cell extracts were loaded in ultracentrifuge tubes (Beckman). Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10% of the lysate was used to extract DNA by AMPure SPRI beads (Beckman Coulter, A63881) and chromatin digestion was verified by resolving on 2% Agarose gel ...
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bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
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bioRxiv - Microbiology 2023Quote: ... PBS-washed cells were PFA-fixed for 90 minutes and immunostained with the following antibodies diluted in 0.1% Triton X-100 in PBS: anti-HIV-1-core-antigen-FITC or -PE (#6604665 or #6604667; Beckman Coulter), rabbit-anti-human-MX1/2 (#sc-166412 ...
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bioRxiv - Microbiology 2023Quote: ... followed by flow cytometry analysis of CAp24 antigen expression on fixed and permeabilized cells labelled with KC57-fluorescein isothiocyanate (FITC) (Beckman Coulter).
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bioRxiv - Molecular Biology 2022Quote: ... a constant volume of extract was layered onto an ultracentrifuge tube (tube 13.2mL-Beckman Coulter SW-41) containing a continuous 10-40% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane extract was clarified by spinning at 10,000 g for 30 minutes (Beckman Coulter JA-20 rotor). The supernatant was diluted 3 times with NiNTA A buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane extract was clarified by spinning at 10,000 RCF for 30 minutes (Beckman Coulter JA-20 rotor). The supernatant was diluted 3 times with NiNTA A buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Molecular Biology 2019Quote: ... The extracts were subjected to ultracentrifugation at 70 krpm for 15 min on a TLA100.3 rotor (Beckman-Coulter) to remove ribosomes ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane extract was clarified by spinning at 10,000 RCF for 30 minutes (Beckman Coulter JA-20 rotor). The supernatant was diluted 3 times with NiNTA A buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane extract was clarified by spinning at 10,000 RCF for 30 minutes (Beckman Coulter JA-20 rotor). The supernatant was diluted 3 times with NiNTA A buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Microbiology 2021Quote: ... and stained for 30min at room temperature with 1:160 dilution of PE-conjugated mouse-anti-human Ab to HIV-1 p24 and core antigens (Beckman Coulter, Fullerton, CA). Fluorescent profiles were recorded using a Guava easyCyte and InCyte software.
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bioRxiv - Immunology 2020Quote: ... Virus replication kinetics was monitored by SIV Gag p27 production in the supernatant using an SIV core antigen ELISA assay kit (Beckman Coulter, Miami, FL). To assess the properties of SHIV-Ag85B ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was centrifuged at 200,000 g for 1 h at 4 °C (Beckman Coulter Optima LE-80K). The membrane pellet was homogenized and solubilized in 20 mM HEPES (pH 7.8 ...
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bioRxiv - Cell Biology 2021Quote: ... The crude extract was clarified by high-speed centrifugation (60 min at 36,000 ×g at 4 °C) by Beckman Coulter centrifuge.
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bioRxiv - Plant Biology 2023Quote: ... Extract was then centrifuged at 40 000 rpm for 2 hours at 4°C using the 50Ti rotor (Beckman). A protocole previsouly used in the laboratory and described in Maclot et al ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting cell extracts were layered over a 20% sucrose cushion (polysome buffer + 20% w/v sucrose) and ultracentrifuged (Beckman Coulter TLA100.3 Fixed-Angle Rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... the extract and the protein ladder were overlaid onto separate gradients and centrifuged with an SW41Ti rotor (Beckman Coulter, 20U113807) in an Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... The microsomal (Micro) and cytoplasmic (Cyto) fractions were collected by centrifugation of soluble cell extract in a TLA- 110 rotor (Beckman Coulter ultracentrifuge) at 100,000 x g for 30 minutes at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... DNA extracts were purified using a bead clean up method with a 2:1 ratio of Ampure XP beads (Beckman Coulter, USA) to DNA elution ...
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bioRxiv - Microbiology 2024Quote: Extracts were then diluted 20-fold (in 33% acetic acid) and their absorbance at A595 determined using a spectrophotometer (Beckman Coulter, DU-800).