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Citations for Beckman :
1 - 50 of 120 citations for Interleukin 35 IL 35 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and 35S was determined using a LS6500 Multipurpose Scintillation Counter (Beckman Coulter). IVT ...
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bioRxiv - Biochemistry 2020Quote: ... Crosslinking reactions were loaded on 600µl 5∼35% glycerol gradients (Beckman 344090 tubes) in BC300N buffer and centrifuged for 4hr at 55,000rpm at 4°C in a TLS-55 rotor (with Beckman 356860 adaptors) ...
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bioRxiv - Genetics 2021Quote: ... 20 ul of sample was taken in duplicate for 35S count taken by Beckman scintillation counter ...
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bioRxiv - Physiology 2023Quote: ... and the amplified library was cleaned up with 35 μL AMPureXP beads (Beckman Coulter). The library was sequenced with 75 bp paired-end mRNA reads on an Illumina NextSeq500 platform with a Mid Output 150 flowcell (Illumina).
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bioRxiv - Microbiology 2023Quote: ... Duplicate samples were pooled and cleaned using 35 uL of SPRI beads from Beckman Coulter® Life Sciences.
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bioRxiv - Biochemistry 2022Quote: ... followed by ultracentrifugation at 18,000g for 35 min at 4 °C (Optima XPN, Beckman Coulter). The clarified supernatant was loaded through a sample pump of an NGC Quest 10 Chromatography System (Bio-Rad Laboratories ...
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bioRxiv - Systems Biology 2023Quote: ... we purified the libraries using 0.7x (35 μL) AMPure XP magnetic beads (Beckman Coulter, A63881) followed by two 80% ethanol washes and a final elution of our purified barcodes in 20 μL of nuclease-free water per sample ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was performed at 50,000xg for 35 min in a SW 32.1Ti rotor (Beckman Coulter). Crude vacuoles were isolated from the top layer and collected using a pipet (∼1 mL ...
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bioRxiv - Biophysics 2019Quote: ... The lysate was spun for 35 min at 35000 rpm at 4°C (Ti70 rotor, Beckman Coulter) and the supernatant was filtered through a 5 µm syringe filter (Millex ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified by centrifugation at 35 Krpm for 30 min in a 45ti rotor (Beckman). The supernatant was filtered (0.45 um ...
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bioRxiv - Microbiology 2023Quote: ... Inoculated panels were incubated at 35°C for 18 h in MicroScan Walkway40 Plus (Beckman Coulter Inc.) or MicroScan Walkway DxM 1096 (Beckman Coulter Inc.) ...
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bioRxiv - Neuroscience 2024Quote: ... This was carefully layered on top of 2ml 35% iodixanol in a clear polycarbonate tube (Beckman 355672) and centrifuged at 10,000x g for 30 min at 4C with deceleration turned off ...
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bioRxiv - Biophysics 2020Quote: ... and then sonicated and ultracentrifuged (440,000 g, 35 min, 4°C; MLA80 rotor, Beckman Optima™ MAX-TL). The supernatant was applied to a Ni-NTA column (Machery-Nagel ...
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bioRxiv - Microbiology 2020Quote: ... and separated in potassium tartrate-glycerol gradient 10–35% for 2 hrs at 175,000 ×g (Beckman SW41 Ti rotor). Purified viral particles were suspended in 10 µg/ml BSA solution or 10 µg/ml CbAE-1 solution and incubated for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... and the amount of released [35S]-labeled N-formyl-methionine was measured using a scintillation counter (Beckman Coulter, Inc.) in 3.5 ml of Econo-Safe scintillation cocktail (RPI) ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were mounted on bottomless 35 mm dishes with a 7 mm microwell using silicone vacuum grease (Beckman Coulter). Conditioned media from the coverslip’s well ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μl M2 eluate was added to the top of a 12 ml 15 - 35 % glycerol gradient and centrifuged in an SW40Ti rotor (Beckman) at 40 ...
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bioRxiv - Microbiology 2021Quote: ... ‘Ex− medium’ was prepared by sedimenting 35 ml of ‘RPMI+’ medium supplemented with 20 % FBS by ultracentrifugation in a SW32 or SW28 swing-out rotor (Beckman) at 4 °C for at least 16 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Percoll in STE and loaded on six linear 10-35% Percoll gradients in STE in polycarbonate tubes for SW28 rotor (Beckman). Gradients were centrifuged for 1 h at 24,000 rpm ...
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bioRxiv - Biophysics 2020Quote: ... Thylakoids were obtained from the supernatant cell homogenate by ultracentrifugation (35 min at 40,000 rpm in a Beckman Ti45 rotor) and thylakoid-containing pellets were suspended in Chelex-100-treated breaking buffer to a concentration of 1.0-1.5 mg of Chl mL-1 with [Chl] determined from methanol extracts according to (54) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The pellet was resuspended in 60% Percoll in STE and loaded on the bottom of six 10-35% Percoll gradient in STE in polycarbonate tubes for SW28 rotor (Beckman). Gradients were centrifuged for 1 hour at 24000 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant from this step was then subjected to ultracentrifugation at 200,000g for 35 min (Beckman Type 45 Ti rotor) to collect microsomes ...
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bioRxiv - Biochemistry 2023Quote: ... made in TEMB buffer) and ultracentrifuged at 105,000 x g for 35 min (SW 32 Ti Swinging-bucket, Beckman Coulter). Gradients were fractionated ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 40,000 rpm for 35 min at 4°C (Type 45 Ti Rotor, Beckman Coulter). The resulting supernatant was mixed with 1ml pre-washed IgG Sepharose 6 FastFlow beads (Cytiva ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were centrifuged at 35 000 RPM for 18 hours at 4°C in a 70Ti rotor and L8-M ultracentrifuge (Beckman Coulter). The ribosome pellets were then gently washed with RNase-free water and resuspended in 300 μl of resuspension buffer (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged through a 10–35% sucrose gradient for 19 h at 16,000 rpm in a SW28 rotor (Beckman Coulter, Inc.). Fractions containing the 70S peaks were pooled and kept at −80℃ for further use.
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bioRxiv - Biochemistry 2020Quote: ... and the amount of released [35S]-labeled dipeptide was measured for each time point using a scintillation counter (Beckman Coulter, Inc.).
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bioRxiv - Microbiology 2021Quote: ... Cell supernatants containing the pseudotype viral particles were centrifuged at 109,000 g or 35 min at 4 °C using a TLA100.3 rotor with an Optima TLX ultracentrifuge (Beckman Coulter, CA, USA), and the pellet was then lysed for western blotting analysis.
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bioRxiv - Biophysics 2022Quote: ... the 30S and 50S subunits were separated on a 15-35% (w/v) sucrose gradient in dissociation buffer on a SW-32 rotor (Beckman-Coulter) spun at 28,000 rpm (97,000 xg ...
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bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged at 35 000 rpm for 18 h in a Beckman SW41 rotor in an OptimaXE-90 Ultra-centrifuge (Beckman Coulter). Eleven fractions were manually collected from each tube ...
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bioRxiv - Genomics 2023Quote: ... except for the pre-PCR clean-up step where DNA was cleaned with 35 μL (0.76×) of AMPure XP beads (Beckman Coulter, A63881). 12 cycles of PCR were used to amplify the ChIP-seq library ...
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bioRxiv - Microbiology 2021Quote: ... density gradient (35 to 0% in PBS) and ultracentrifuged at 200,000 × g for at least 12 hours at 4 °C (Beckman Optima XL-100K). The corresponding clear yellow band was collected ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
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bioRxiv - Bioengineering 2021Quote: ... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
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bioRxiv - Biochemistry 2021Quote: ... Cytomics FC 500 (Beckman Coulter) or CytoFLEX (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: ... FCS files were exported using CytExpert Software (Beckman Coulter). Values for forward scatter (FSC area ...
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bioRxiv - Cancer Biology 2020Quote: ... on a Cytotomics FC 500 (Beckman Coulter, Brea, CA) flow cytometer ...
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bioRxiv - Cancer Biology 2023Quote: ... Debarcoded FCS files were uploaded to Cytobank (Beckman Coulter), gated for Gaussian parameters ...
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bioRxiv - Cell Biology 2023Quote: ... and analyzed by flow cytometry (Cytomics FC 500, Beckman-Coulter) for the residual fluorescence in dividing cells ...
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bioRxiv - Immunology 2020Quote: ... during 30min and then transferred into a DuraClone IF T Helper cell tube (CD3, CD4, IL-4, IL-17A, IFNγ) (Beckman Coulter), using the IntraPrep fixation permeabilization kit (Beckman Coulter) ...
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bioRxiv - Systems Biology 2021Quote: HeLa Kyoto and R1/E mESCs (gifts from Hyman lab, Dresden) and HEK293 (gift from Beckman lab, Berlin), Ptk2 cell lines (gift from Simons lab ...
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bioRxiv - Cancer Biology 2023Quote: ... FCS files were analyzed using CytExpert version 2.2 software (Beckman Coulter).
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bioRxiv - Cancer Biology 2023Quote: FCS files were analyzed using CytExpert version 2.2 software (Beckman Coulter). Dead cells (Annexin V+/PI+ cells ...
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bioRxiv - Cell Biology 2022Quote: ... with analysis performed on a flow cytometer (Beckman Coulter Cytomics FC 500). hPSCs were labelled with pluripotency markers (SSEA-4 ...
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bioRxiv - Immunology 2020Quote: ... Cells were analyzed by flow cytometry (FC 500, Beckman Coulter, Brea, USA) and data were analyzed with the CFX software package (Beckman Coulter).
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bioRxiv - Cancer Biology 2023Quote: ... Normalized fcs datafiles were then exported and uploaded to Cytobank software (Beckman Coulter). Each file was cleaned up by a series of manually set gates to exclude normalization beads ...
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bioRxiv - Biochemistry 2023Quote: ... The stained cells were analysed on a Cytomics FC 500 flow cytometer (Beckman) (FL1 /FL2 /FL3 /FL4 /FL5 ...
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bioRxiv - Immunology 2021Quote: Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter). Test sera were diluted 1:40 in growth medium and serially diluted ...
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bioRxiv - Developmental Biology 2023Quote: The anti–human uncoupled primary antibodies used are listed: anti-human CD34 (Beckman, QBEnd/10), anti-human ACE (BB9 ...
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bioRxiv - Immunology 2022Quote: ... all from BD Biosciences as well as IL-2 PE (clone N7.48A, Beckman, Cat IM2718U) and TNF PCP-Cy5.5 (clone MAb11 ...