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Citations for Beckman :
1 - 44 of 44 citations for Interleukin 23 Mouse Sf9 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and Sf9 cell lysate at 50,000 rpm in Ti70 rotor (Beckman Coulter) for 30-45 minutes.
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bioRxiv - Plant Biology 2024Quote: ... Preamplification of cDNA used 23 PCR cycles and was purified using Agencourt Ampure XP beads (Beckman Coulter) with a modified bead ...
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bioRxiv - Cell Biology 2022Quote: ... milk was prevented from cooling down and was processed within 20 minutes after collection by 2 rounds of centrifugation at 3,000xg for 10 minutes at 23°C (Beckman Coulter Allegra X-12R ...
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bioRxiv - Cell Biology 2023Quote: ... was collected before ultracentrifugation at 100,000 g for 30 min at 23°C in a TLA100.3 rotors (Beckman & Coutler). Supernatant was discarded and pellet fractions were obtained by resuspending pellet in Laemmli buffer 1X with volume equal to the initial volume ...
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bioRxiv - Cell Biology 2023Quote: ... was collected before ultracentrifugation at 100,000 g for 30 min at 23°C in a TLA100.3 rotors (Beckman & Coutler). Supernatant was discarded and pellet fractions were obtained by resuspending pellet in Laemmli buffer 1X with volume equal to the initial volume ...
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bioRxiv - Biophysics 2022Quote: SpeMreBs with a volume of 200 μL were polymerised for 1 to 6 h and centrifuged (100,000 rpm, 23°C, 120 min) in a TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 200 μL of water ...
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bioRxiv - Cell Biology 2020Quote: ... This crude mitochondrial pellet was further purified on a discontinuous percoll gradient (buffer composition 15/23/40% sucrose) by centrifugation (40,000 x g, 1hr, 4°C) in an SW41 rotor (Beckman Coulter) and enriched mitochondria were collected from the 23-40% interface (adapted from [76]) ...
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bioRxiv - Biophysics 2023Quote: SpeMreBs in buffer S polymerized at room temperature with a volume of 200 μL were polymerized for 1–6 h and centrifuged (100,000 rpm at 23 °C for 120 min) in a TLA-100 rotor (Beckman Coulter) (2) ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 1 hr at RT before centrifugation at 100,000 x g (23°C) in a TLA100 rotor (Beckman-Coulter). The top 50% of the supernatant was removed ...
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bioRxiv - Cell Biology 2023Quote: ... the fifth virus was freeze-thawed three times and ultracentrifuged at 23 k rpm at 4 °C for 1.5 hours in the Super-centrifuge Optima XE-90 Ultracentrifuge (Beckman Coulter) using Ultra-Clear Centrifuge Tubes (Beckman Coulter) ...
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bioRxiv - Neuroscience 2022Quote: ... The crude viral lysate was purified with two rounds of CsCl gradient ultracentrifugation at 100,000×g and 4°C for 23 h using 13.5-mL Quick-Seal tubes and a NVT65 rotor (Beckman Coulter, Brea, CA). Each tube was placed gently and 500– 750 µL of white turbid bands were collected ...
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bioRxiv - Biophysics 2020Quote: Sedimentation velocity experiments were performed as described (23, 24, 32) with an Optima XL-A analytical ultracentrifuge and An5OTi rotor (Beckman Instruments, Fullerton, CA) at 42000 rpm in buffer H ...
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bioRxiv - Cell Biology 2023Quote: ... IgG2a Mouse-PE (A09142, Beckman Coulter), IgG1κ Mouse-AF647 (565571 BD Biosciences) ...
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bioRxiv - Systems Biology 2020Quote: ... tetramethylrhodamine (TRITC)-conjugated anti-mouse IgM (#731781, Beckman Coulter), and Alexa-594-conjugated anti-rabbit IgG (A11012 ...
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bioRxiv - Immunology 2023Quote: ... for mouse samples or a CytoFLEX LX (Beckman Coulter) for human samples ...
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bioRxiv - Microbiology 2020Quote: ... fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal antibody KC57 (Beckman Coulter) was used at a dilution of 1:100.
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bioRxiv - Cell Biology 2023Quote: ... secondary anti-mouse-HRP (Beckman Coulter, Ref. 715-035-151), secondary anti-rabbit-HRP (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... mouse anti-CD79b PE (clone CB3-1) (IM1612U) was from Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... mouse-anti-CD159a-PE (Beckman Coulter, catalog #IM3291U, dil 1:30), and mouse-anti-CD14-BV650 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... including PE-Cy7-conjugated mouse anti-human CD45 (Beckman Coulter, CA, USA), efluor660-conjugated rat anti-human OCT3/4 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Corresponding isotype controls were as follows: IgG1 Mouse-PE (A0779, Beckman Coulter), IgG2a Mouse-PE (A09142 ...
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bioRxiv - Immunology 2019Quote: The following antibodies were used: anti-p24-FITC (mouse monoclonal, Beckman and Coulter, #KC-57); anti-CD3-Pacific Blue (mouse monoclonal ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with primary anti-HIV-Gag antibody (Beckman-Coulter; KC57-RD1, mouse derived), and with α-mouse Alexa Fluor555-conjugated secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with primary anti-HIV-Gag antibody (Beckman-Coulter; KC57-FITC, mouse derived), and cells were acquired using the MACs Quant Flow Cytometer (Milentyi ...
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bioRxiv - Cell Biology 2021Quote: ... platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin ...
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bioRxiv - Cell Biology 2021Quote: Platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Incubation was performed at room temperature for 10 minutes in the dark ...
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bioRxiv - Microbiology 2021Quote: ... and 10μl of PE-conjugated mouse-anti-human langerin Ab (Clone DCGM4; Beckman Coulter, Marseille, France). In other experiments ...
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bioRxiv - Microbiology 2019Quote: ... A portion of each sample was stained with Mouse-Anti Human CD15-PE (Beckman Coulter, USA) and run under BD Accuri C6 Flow Cytometer ...
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter; 1:100 dilution). The percentage of transfected or infected cells (p24+ ...
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bioRxiv - Immunology 2022Quote: ... The occult blood in mouse fecal samples was detected using Hemoccult Test Kit (Beckman Coulter Inc, Fullerton, CA).
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from wild-type mouse livers using the Agencourt RNAdvance Tissue kit (Beckman Coulter, Brea, CA), and cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) in combination with Nef or Vpu rabbit antisera (1:1000 dilution) ...
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bioRxiv - Genomics 2022Quote: ... PCR products with no hashtag libraries (spleen, mouse lung, and human lung cells) were combined and purified twice with 0.65× AmPure XP beads (Beckman Coulter) and eluted with 21 μL of nuclease-free water ...
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bioRxiv - Immunology 2021Quote: ... as assessed by flow cytometry with Korman orange-conjugated mouse anti-human CD19 monoclonal antibody (mAb) (Beckman Coulter, CA, USA). The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza ...
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bioRxiv - Microbiology 2023Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA, 1:100 dilution). Flow cytometry measurements were performed using a FACSCalibur (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Putative LSC-enriched populations were isolated from freshly expanded MLL-AF9 mouse leukemia cells by sorting c-Kithigh (top 10%) cells on a MoFlo XDP cell sorter (Beckman Coulter). Sorted cells were plated in triplicate in semi-solid methylcellulose medium (Methocult M3434 ...
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bioRxiv - Pathology 2023Quote: ... the proportion of HIV-1-infected cells was determined by intracellular staining of the viral protein Gag-p24 with a Mouse anti-p24-FITC antibody (clone KC57, Beckman Coulter).
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bioRxiv - Immunology 2023Quote: ... These were chosen to stain splenocytes and lymphocytes from the NALT of the same mouse for the following TCR Vβ segments (clone, brand, fluorophore) to identify any overlap of clonotypes: VB1 (BL37.2, Beckman Coulter, PE), VB2 (RE654 ...
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bioRxiv - Immunology 2024Quote: ... The sections were washed and incubated for two hours with anti-human mouse CD14 Texas Red (clone: RMO52) (Beckman Coulter, USA). Afterward ...
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bioRxiv - Cancer Biology 2024Quote: Analysis of GALNT3 or fibronectin expression on human or mouse pancreatic cancer cells was performed using a flow cytometer (Gallios, Beckman Coulter) with appropriate antibodies ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal antibodies recognizing the closed conformation of β1 integrin (unoccupied β1 integrin, termed ‘inactive’) were mouse anti-human β1 integrin 4B4 (Beckman Coulter, 6603113) and rat anti-human β1 integrin mAb13 (generated in house from a hybridoma) ...
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bioRxiv - Microbiology 2019Quote: ... Permeabilized cells were then incubated with 100 μl of 1:100 dilution of phycoerythrin-conjugated mouse anti-p24 mAb (KC57-RD1; Beckman Coulter, Inc.) for 30 min at 4 °C with intermittent mixing ...
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bioRxiv - Microbiology 2021Quote: ... and stained for 30min at room temperature with 1:160 dilution of PE-conjugated mouse-anti-human Ab to HIV-1 p24 and core antigens (Beckman Coulter, Fullerton, CA). Fluorescent profiles were recorded using a Guava easyCyte and InCyte software.