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Citations for Beckman :
1 - 50 of 908 citations for Human V Set And Immunoglobulin Domain Containing Protein 2 VSIG2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: The ACCESS SARS-CoV-2 CLIA (Beckman Coulter Inc., California, USA) was used for semiquantitative detection of IgG directed against S protein RBD using serum obtained from venipuncture blood ...
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bioRxiv - Immunology 2021Quote: ... Immunoglobulin bound bacteria were analyzed by CytoFLEX (Beckman coulter). Experimental colitis For acute experimental colitis ...
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bioRxiv - Cancer Biology 2020Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Then small molecules were introduced from stock plates with a 384-well formatted set of 50 nL stainless steel pin-tool (V&P Scientific) on a Biomek FXP (Beckman) automation workstation to 10 μM final concentration (in 0.1% DMSO) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Developmental Biology 2019Quote: Detection of apoptotic TCam-2 cells was performed 48 h after transfection of TCam-2 cells with control and KIF18A siRNA constructs using an Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Supernatant containing proteins and membrane were ultracentrifuged at 200,000xg (Beckman Coulter Ultracentrifuge ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were immobilized on a magnetic rack and the supernatant containing the desired DNA to be sequenced was taken and purified using 1X v/v SPRI Select magnetic beads (Beckman Coulter, Brea, CA, Cat # B23317) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: The TCR V beta repertoire kit contained antibodies to over 76% of V βeta families (Beckman Coulter, Fullerton, CA). The cells were stained with these antibodies in combination with tetramers or dextramers for 20 minutes at RT to determine the TRBV repertoire of antigen specific cells ...
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bioRxiv - Cell Biology 2021Quote: ... Apoptosis was analyzed using Annexin V-FITC / 7-AAD kit (Beckman Coulter) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... 2) first-round PCRs were set up using a Biomek FX liquid handling system with a 96- well head (Beckman Coulter); 3 ...
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bioRxiv - Microbiology 2022Quote: ... The immunoturbidimetric test was used to assess serum complement (C) and immunoglobulin antibodies (AU5421; Beckman Coulter, USA). Immunoblotting and immunofluorescence were used to detect serum autoantibodies ...
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bioRxiv - Neuroscience 2019Quote: ... iodixanol solution and overlaid with 7.0 mL of 25% (v/v) iodixanol and 350 µL of 5% (v/v) iodixanol in a polyalomar tube (Beckman, Miami, FL). All of the above iodixanol solutions were prepared in lipid flotation buffer (10 mM HEPES ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... acceleration was set at Max and deceleration was set at Coast (no brake) setting (Beckman Coulter-Optima XPN 100 ultracentrifuge). Ribosome pellets were resuspended in 750 μL of resuspension buffer (20 mM Tris-Cl pH 7.5 ...
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bioRxiv - Bioengineering 2023Quote: ... and cleaned with cleanup mix containing SPRIselect Reagent Kit (Beckman Coulter) in between steps ...
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bioRxiv - Cancer Biology 2023Quote: ... the stage of apoptosis was assessed using the Annexin V FITC kit (Beckman Coulter, IM2375) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mL of 20% (w/v) sucrose in DPBS (-Ca, -Mg) is prepared in ultracentrifuge tubes (Beckman Coulter, 344058) to which the divided EnvA- pseudotyped viral media is added before pelleting (20,000 RPM for 2 hours at 4°C) ...
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 rotor (Beckman).
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bioRxiv - Immunology 2024Quote: ... PCR products were purified using 1.5x v/v AMPure XP (Beckman Coulter) with 80% ethanol washes and eluted into 25 μL of water for downstream processing ...
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bioRxiv - Immunology 2024Quote: ... PCR products were purified using 0.7x v/v AMPure XP (Beckman Coulter) with 80% ethanol washes and eluted into 10 μL of water for downstream processing ...
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bioRxiv - Microbiology 2022Quote: ... The V4 region of 16S rRNA gene was amplified using the universal primer set 515F and 806R.27 PCR products were cleaned up with an AMPure XP kit (Beckman Coulter, CA), followed by electrophoresis on 0.7% agarose gel and 1.15% Synergel (Diversified Biotech ...
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bioRxiv - Cell Biology 2019Quote: ... 30% 40% (v/v) glycerol solutions in 14×89 mm tubes (Beckman Instruments). The glycerol solutions were prepared in 20 mM Hepes pH 7.4 ...
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bioRxiv - Genomics 2019Quote: ... followed by clean-up using 1x v/v AMPure XP beads (Beckman Coulter). Sequencing adaptors ...
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bioRxiv - Cancer Biology 2023Quote: ... For cell apoptosis assay, Annexin V-PI apoptosis detection kit (Bioscience, A6030L) and Flow cytometry (Beckman, USA) were used according to the manufacturer’s instruction.
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bioRxiv - Immunology 2023Quote: ... labelled BM-MDSCs were stained with the Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter, IL, Italy) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 or SW28 rotor (Beckman, Indianapolis, IN). Alternatively ...
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bioRxiv - Genomics 2020Quote: ... Libraries were size selected using 0.7x-0.9x (v/v) AMpure XP beads (Beckman Coulter) to final length of 200-500 bp and sequenced by NextSeq 500 using 60 sequencing cycles for Read 1 and 15 cycles for Read 2.
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bioRxiv - Cell Biology 2023Quote: ... The resulting DNA was purified using 1x v/v AMPure XP (Beckman Coulter, A63881) and annealed at 50 °C for 1 hour in the presence of 50 mM NaCl and 100 nM splint oligo ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.9) were set up in 14 × 95 mm tubes (Beckman Coulter). 166 μl reactions were assembled as described previously for Benzonase-treated affinity pull-down assay ...
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bioRxiv - Cell Biology 2020Quote: ... 30% Percoll (v/v) (pH 7.4)) by ultracentrifugation (SW55-Ti rotor, Optima-XE100 ultracentrifuge, Beckman Coulter) at 95,000g for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was purified and concentrated using 1X v/v SPRI Select beads (Beckman Coulter, Cat # B23317) and eluted in 50 μL of water according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... MT seeds were diluted in BRB80 containing 2 μM taxol and centrifuged at 100,000 g (Optima TL100; Beckman Coulter) for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
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bioRxiv - Biophysics 2019Quote: Sedimentation velocity data of the N-terminal RBCC domain of KAP1 (0.5 mg/mL) were obtained using a Beckman Optima XL-I analytical ultracentrifuge (Beckman Coulter) with an absorbance optical system ...
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bioRxiv - Microbiology 2022Quote: ... Samples fixed in formaldehyde solution were diluted to 1% (v/v) in filtered IsoFlow Sheath Fluid (Beckman). Of this ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EGTA and 30% Percoll (v/v)) in 14 × 89-mm centrifuge tubes (cat. 344059, Beckman). The tubes were then centrifuged on a SW 41 Ti rotor (Beckman ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked in 2% human serum and PBS for 30 min at 4°C and stained with DC-SIGN (Beckman Coulter #A07407), CD80 (BioLegend #305214) ...
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bioRxiv - Molecular Biology 2023Quote: ... stained the cells by a Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, A211-01) and detected cells distribution using a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter).
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bioRxiv - Biophysics 2021Quote: ... solubilized supernatant was collected and subjected to sucrose gradient ultracentrifugation (10% to 50% (w/v) sucrose in buffer A with 0.03% (w/v) digitonin) at 35000 rpm (sw41 Ti rotor, Beckman Coulter) for 16 hours at 4℃ ...
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bioRxiv - Microbiology 2021Quote: ... and 1.3 mL of 5% (v/v) glycerol in TNE buffer) in 5 mL thin-walled Ultraclear centrifuge tubes (Beckman Coulter). Viral nucleocapsids were pelleted by centrifugation at 23,000 rpm in an SW 55 Ti rotor for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 1x BRB80 solution supplemented with 60 % v/v glycerol and 0.5 mM GMPCPP in a 230 μL thick-walled centrifuge tube (Beckman-Coulter). Care was taken to maintain the two distinct liquid phases ...
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bioRxiv - Cancer Biology 2020Quote: ... cellular nucleic acids from each lysed single cell were extracted from TCL lysis buffer using a 2.2x (v/v) RNA SPRI (RNA-clean AMPure beads, Beckman-Coulter). After ...
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bioRxiv - Bioengineering 2020Quote: ... microbubbles rested for 10 min before the suspension was mixed and diluted to 0.2% v/v in Isoton (Beckman-Coulter). For each vial ...
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bioRxiv - Biochemistry 2019Quote: ... in iso-osmolar RB150/Mg2+ (containing a reduced level (2%) of glycerol) and 80µl were transferred to 7 × 20 mm polycarbonate tubes (Beckman Coulter, Brea CA), overlaid with 80µl of 35% ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the two amplifications obtained (one per set) were purified with AMPure XP beads (Beckman-Coulter) at a 1:1 ratio according to the manufacturer’s recommendations and pooled at equimolar concentrations ...
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bioRxiv - Cell Biology 2019Quote: ... apoptotic and necrotic cells with YO-PRO-1 / PI in parallel with a Annexin V / PI staining realized with the Annnexin-V-FITC when needed (Immunotech, Beckman-Coulter). All samples were measured and analyzed on a FACS Calibur 4C as previously described (23 ...
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bioRxiv - Cell Biology 2019Quote: ... apoptotic and necrotic cells with YO-PRO-1 / PI in parallel with a Annexin-V / PI staining realized with the Annnexin-V-FITC when needed (Immunotech, Beckman-Coulter). All samples were measured and analyzed on a FACS Calibur 4C as previously described (73 ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... 1.6 ml of 35% (w/v) sucrose and 5% (w/v) sucrose in MBS were layered in a 5.1 ml ultracentrifuge tube (Beckman Coulter, #326819) to form a discontinuous sucrose gradient ...