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Citations for Beckman :
1 - 50 of 707 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... HIV-1 p24 was stained with Anti-HIV-1 p24-PE (KC57) (Beckman Coulter) for 30 min on ice and washed again 3 times before measuring on the BD FACSCelesta™.
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bioRxiv - Immunology 2022Quote: ... and anti-HIV-1 p24 PE (Beckman coulter) were used ...
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bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with the following p24 antibodies: HIV-1 p24 clone 28B7-APC (Medimabs) and HIV-1 p24 clone KC57-PE (Beckman Coulter). Acquisition was performed on the Fortessa LSR flow cytometer (BD Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... and stained for 30min at room temperature with 1:160 dilution of PE-conjugated mouse-anti-human Ab to HIV-1 p24 and core antigens (Beckman Coulter, Fullerton, CA). Fluorescent profiles were recorded using a Guava easyCyte and InCyte software.
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bioRxiv - Immunology 2022Quote: ... HIV-1 p24 core antigen KC57: 6604667 from Beckman Coulter and APC HIV-1 (p24 ...
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bioRxiv - Immunology 2020Quote: ... and RD1-conjugated anti-HIV-1 p24 (KC57, Beckman Coulter) to identify infected cells ...
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bioRxiv - Microbiology 2023Quote: ... and HIV-1 core antigen-FITC (KC57, Beckman Coulter; 6604665).
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bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
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bioRxiv - Microbiology 2021Quote: ... PE) and HIV-1 Gag core antigen (FH190-1-1, Beckman Coulter; PE, FITC). All samples were acquired on either an BD Fortessa X20 or LSR II using BD FACSDiva software and analyzed using FlowJo v10 (Tree Star) ...
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bioRxiv - Microbiology 2021Quote: ... using anti-HIV-1 p24-PE antibody (#6604667, 1:1000 dilution) purchased from Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... and anti-HIV-1 p24 clone KC57-RD1 (Beckman Coulter, CA, USA). The secondary goat anti-mouse and anti-rabbit Abs conjugated to Alexa488 ...
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bioRxiv - Microbiology 2020Quote: ... staining with anti-HIV-1 Core Antigen Clone KC57 FITC (Beckman Coulter) and detection of HIV-1-infected cells by flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... Cells were probed for HIV-1 core antigen-FITC KC57 (Beckman Coulter) for 1 hr at 4°C then washed with PBS and resuspended in FACS buffer (5% FBS in PBS) ...
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bioRxiv - Immunology 2021Quote: ... (Thermo Fisher Scientific)] and HIV-p24 (KC57, Beckman Coulter). Cells were fixed using 2% paraformaldehyde ...
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bioRxiv - Microbiology 2020Quote: ... and p24 antibodies (anti-HIV core antigen: clone KC57, Beckman Coulter, 1:200).
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bioRxiv - Microbiology 2020Quote: ... The dyes used were: KC57 anti-HIV-1 core antigen-PE (Beckman coulter CO604667), Live/Dead fixable Far Red Dead Cell Stain Kit (Invitrogen L10120 ...
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bioRxiv - Microbiology 2021Quote: Antibodies used for flow cytometry: anti-HIV-1 p24-PE (KC57-RD1 Beckman Coulter); anti-HIV-1 Env clone PGT151 (gift from Laura McCoy ...
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bioRxiv - Immunology 2020Quote: ... HIV-1 p24-PE antibody (KC57-RD1, #6604667) for intracellular staining was purchased from Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24) antibody (1:200) (Beckman Coulter) or a mixture of both antibodies ...
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bioRxiv - Microbiology 2021Quote: ... HIV-p24 PE (KC57) (Beckman Coulter, Fullerton, CA, USA), CD3 Pacific blue (UCHT1) ...
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bioRxiv - Immunology 2020Quote: ... stained intracellularly with anti-HIV-Gag Kc57-RD1 (Beckman Coulter), and analyzed on a BD LSRFortessa™ flow cytometer.
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bioRxiv - Immunology 2020Quote: ... and staining intracellularly with anti-HIV-Gag Kc57-RD1 (Beckman Coulter), and analyzed on a BD LSRFortessa™ flow cytometer ...
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bioRxiv - Immunology 2021Quote: ... following by staining for 15 minutes at room temperature with a 1:100 dilution of a fluorescent anti-HIV-1 GAG antibody (KC57-FITC, Beckman Coulter reference 6604665) in permeabilization buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Microbiology 2023Quote: ... PBS-washed cells were PFA-fixed for 90 minutes and immunostained with the following antibodies diluted in 0.1% Triton X-100 in PBS: anti-HIV-1-core-antigen-FITC or -PE (#6604665 or #6604667; Beckman Coulter), rabbit-anti-human-MX1/2 (#sc-166412 ...
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bioRxiv - Microbiology 2021Quote: ... 50 μL of each cell sample was mixed with 1 μL of KC57-FITC antibody that identifies the HIV-1 Gag and p24 (Beckman Coulter Life Sciences, USA) and incubated at 4°C for 20 min ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with primary anti-HIV-Gag antibody (Beckman-Coulter; KC57-FITC, mouse derived), and cells were acquired using the MACs Quant Flow Cytometer (Milentyi ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with primary anti-HIV-Gag antibody (Beckman-Coulter; KC57-RD1, mouse derived), and with α-mouse Alexa Fluor555-conjugated secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... APC-A750 anti-human CD90 (Thy-1/310, #B36121 Beckman Coulter) and Vioblue anti-human CD45 antibodies (REA747 ...
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bioRxiv - Microbiology 2021Quote: The following fluorochrome-conjugated Abs were used for flow cytometry analysis: HIV-p24 FITC (KC57) (Beckman Coulter, Fullerton, CA, USA), HIV-p24 PE (KC57 ...
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bioRxiv - Microbiology 2024Quote: ... The virus was enriched by ultracentrifugation (Beckman Coulter Optima™ L-80XP) at 246,347g for 4 hours at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2020Quote: Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 28) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor) for 2h at 4 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
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bioRxiv - Developmental Biology 2023Quote: The anti–human uncoupled primary antibodies used are listed: anti-human CD34 (Beckman, QBEnd/10), anti-human ACE (BB9 ...
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bioRxiv - Biochemistry 2021Quote: ... The virus-containing filtrate was then further purified using an ultracentrifuge (Optima XPN-90 Ultracentrifuge, Beckman Coulter, USA) with a cycle of 1,000 rpm (116 g ...
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bioRxiv - Immunology 2022Quote: ... anti-human TCR Vβ1 (Beckman Coulter), anti-human CD3 and CD8 antibodies (BD Bioscience).
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bioRxiv - Microbiology 2020Quote: ... The virus was then purified over a 20% sucrose cushion by centrifugation at 35,000 rpm (Beckman Coulter SW41 rotor) for 3 h at 4°C ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Molecular Biology 2020Quote: ... Virus particles in the supernatant were pelleted by ultracentrifugation at 45.000 rpm for 45 min (Beckman 70.1 Ti Rotor) over a pad of 15% sucrose in 10 mM Tris-HCl buffer (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... Virus RNA was extracted with a magnetic bead-based RNA extraction kit (RNAdvance Viral Reagent kit, Beckman Coulter, C63510) on an epMotion® 5073t liquid handler (Eppendorf ...
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bioRxiv - Immunology 2022Quote: Human Vγ9Vδ2 T lymphocytes were stained with FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and APC-labeled anti-human CD3ε mAb (#UCHT1 ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
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bioRxiv - Biophysics 2023Quote: ... Virus-containing supernatants were collected at 48 hours and 72 hours after transfection and concentrated using ultracentrifugation (Ultraclear, Beckman 344058). The transduction capacity of the viral vector product was confirmed by culturing non-transfected HEK293T cells with the virus after 24 hours and assessing via fluorescent imaging.