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Citations for Beckman :
1 - 50 of 730 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... HIV-1 p24 was stained with Anti-HIV-1 p24-PE (KC57) (Beckman Coulter) for 30 min on ice and washed again 3 times before measuring on the BD FACSCelesta™.
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bioRxiv - Immunology 2022Quote: ... and anti-HIV-1 p24 PE (Beckman coulter) were used ...
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bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with the following p24 antibodies: HIV-1 p24 clone 28B7-APC (Medimabs) and HIV-1 p24 clone KC57-PE (Beckman Coulter). Acquisition was performed on the Fortessa LSR flow cytometer (BD Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... and stained for 30min at room temperature with 1:160 dilution of PE-conjugated mouse-anti-human Ab to HIV-1 p24 and core antigens (Beckman Coulter, Fullerton, CA). Fluorescent profiles were recorded using a Guava easyCyte and InCyte software.
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bioRxiv - Immunology 2022Quote: ... HIV-1 p24 core antigen KC57: 6604667 from Beckman Coulter and APC HIV-1 (p24 ...
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bioRxiv - Immunology 2020Quote: ... and RD1-conjugated anti-HIV-1 p24 (KC57, Beckman Coulter) to identify infected cells ...
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bioRxiv - Microbiology 2023Quote: ... and HIV-1 core antigen-FITC (KC57, Beckman Coulter; 6604665).
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bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
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bioRxiv - Microbiology 2021Quote: ... PE) and HIV-1 Gag core antigen (FH190-1-1, Beckman Coulter; PE, FITC). All samples were acquired on either an BD Fortessa X20 or LSR II using BD FACSDiva software and analyzed using FlowJo v10 (Tree Star) ...
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bioRxiv - Microbiology 2021Quote: ... using anti-HIV-1 p24-PE antibody (#6604667, 1:1000 dilution) purchased from Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... and anti-HIV-1 p24 clone KC57-RD1 (Beckman Coulter, CA, USA). The secondary goat anti-mouse and anti-rabbit Abs conjugated to Alexa488 ...
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bioRxiv - Microbiology 2020Quote: ... staining with anti-HIV-1 Core Antigen Clone KC57 FITC (Beckman Coulter) and detection of HIV-1-infected cells by flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... Cells were probed for HIV-1 core antigen-FITC KC57 (Beckman Coulter) for 1 hr at 4°C then washed with PBS and resuspended in FACS buffer (5% FBS in PBS) ...
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bioRxiv - Immunology 2021Quote: ... (Thermo Fisher Scientific)] and HIV-p24 (KC57, Beckman Coulter). Cells were fixed using 2% paraformaldehyde ...
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bioRxiv - Microbiology 2020Quote: ... and p24 antibodies (anti-HIV core antigen: clone KC57, Beckman Coulter, 1:200).
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bioRxiv - Microbiology 2020Quote: ... The dyes used were: KC57 anti-HIV-1 core antigen-PE (Beckman coulter CO604667), Live/Dead fixable Far Red Dead Cell Stain Kit (Invitrogen L10120 ...
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bioRxiv - Microbiology 2021Quote: Antibodies used for flow cytometry: anti-HIV-1 p24-PE (KC57-RD1 Beckman Coulter); anti-HIV-1 Env clone PGT151 (gift from Laura McCoy ...
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bioRxiv - Immunology 2020Quote: ... HIV-1 p24-PE antibody (KC57-RD1, #6604667) for intracellular staining was purchased from Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24) antibody (1:200) (Beckman Coulter) or a mixture of both antibodies ...
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bioRxiv - Microbiology 2021Quote: ... HIV-p24 PE (KC57) (Beckman Coulter, Fullerton, CA, USA), CD3 Pacific blue (UCHT1) ...
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bioRxiv - Immunology 2020Quote: ... stained intracellularly with anti-HIV-Gag Kc57-RD1 (Beckman Coulter), and analyzed on a BD LSRFortessa™ flow cytometer.
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bioRxiv - Immunology 2020Quote: ... and staining intracellularly with anti-HIV-Gag Kc57-RD1 (Beckman Coulter), and analyzed on a BD LSRFortessa™ flow cytometer ...
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bioRxiv - Immunology 2021Quote: ... following by staining for 15 minutes at room temperature with a 1:100 dilution of a fluorescent anti-HIV-1 GAG antibody (KC57-FITC, Beckman Coulter reference 6604665) in permeabilization buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Microbiology 2023Quote: ... PBS-washed cells were PFA-fixed for 90 minutes and immunostained with the following antibodies diluted in 0.1% Triton X-100 in PBS: anti-HIV-1-core-antigen-FITC or -PE (#6604665 or #6604667; Beckman Coulter), rabbit-anti-human-MX1/2 (#sc-166412 ...
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bioRxiv - Microbiology 2021Quote: ... 50 μL of each cell sample was mixed with 1 μL of KC57-FITC antibody that identifies the HIV-1 Gag and p24 (Beckman Coulter Life Sciences, USA) and incubated at 4°C for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... and spun for 1 hour in a MAX-E tabletop ultracentrifuge (Beckman) at 4°C and 100,000 × g (47,000 rpm ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with primary anti-HIV-Gag antibody (Beckman-Coulter; KC57-FITC, mouse derived), and cells were acquired using the MACs Quant Flow Cytometer (Milentyi ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with primary anti-HIV-Gag antibody (Beckman-Coulter; KC57-RD1, mouse derived), and with α-mouse Alexa Fluor555-conjugated secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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bioRxiv - Microbiology 2021Quote: 1 mL pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... APC-A750 anti-human CD90 (Thy-1/310, #B36121 Beckman Coulter) and Vioblue anti-human CD45 antibodies (REA747 ...
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bioRxiv - Cell Biology 2020Quote: ... spinning at 3,200 rpm (Beckman J-6M/E centrifuge). The centrifuge chamber was set at 4°C during all centrifugation steps until approximately 10 min before cell harvesting ...
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bioRxiv - Microbiology 2021Quote: The following fluorochrome-conjugated Abs were used for flow cytometry analysis: HIV-p24 FITC (KC57) (Beckman Coulter, Fullerton, CA, USA), HIV-p24 PE (KC57 ...
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bioRxiv - Microbiology 2024Quote: ... The virus was enriched by ultracentrifugation (Beckman Coulter Optima™ L-80XP) at 246,347g for 4 hours at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Genomics 2021Quote: Beckman Coulter Avanti J-E centrifuge (Beckman Coulter, cat, no. A20698)
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bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2020Quote: Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 28) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor) for 2h at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The lysed cells were pelleted via centrifugation (Beckman Coulter, Avanti J-E) at 40,000 × g for 35 min ...
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bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris was removed by centrifugation at 20,000 rpm at 4°C for 1 h (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 25.50). The supernatant was collected and loaded onto a pre-equilibrated 5 ml His-Trap FF crude column ...
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bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
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bioRxiv - Biochemistry 2022Quote: ... Packed eggs were crushed by centrifugation (BECKMAN, Avanti J-E, JS13.1 swinging rotor) for 20 min at 18,973×g ...
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bioRxiv - Molecular Biology 2020Quote: ... Packed eggs were crushed by centrifugation (BECKMAN, Avanti J-E, JS13.1 swinging rotor) for 20 min at 18973 × g ...