Labshake search
Citations for Beckman :
1 - 50 of 850 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... HIV-1 p24 core antigen KC57: 6604667 from Beckman Coulter and APC HIV-1 (p24 ...
-
bioRxiv - Microbiology 2021Quote: ... PE) and HIV-1 Gag core antigen (FH190-1-1, Beckman Coulter; PE, FITC). All samples were acquired on either an BD Fortessa X20 or LSR II using BD FACSDiva software and analyzed using FlowJo v10 (Tree Star) ...
-
bioRxiv - Microbiology 2023Quote: ... and HIV-1 core antigen-FITC (KC57, Beckman Coulter; 6604665).
-
bioRxiv - Microbiology 2020Quote: ... staining with anti-HIV-1 Core Antigen Clone KC57 FITC (Beckman Coulter) and detection of HIV-1-infected cells by flow cytometry ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were probed for HIV-1 core antigen-FITC KC57 (Beckman Coulter) for 1 hr at 4°C then washed with PBS and resuspended in FACS buffer (5% FBS in PBS) ...
-
bioRxiv - Microbiology 2020Quote: ... and p24 antibodies (anti-HIV core antigen: clone KC57, Beckman Coulter, 1:200).
-
bioRxiv - Microbiology 2020Quote: ... The dyes used were: KC57 anti-HIV-1 core antigen-PE (Beckman coulter CO604667), Live/Dead fixable Far Red Dead Cell Stain Kit (Invitrogen L10120 ...
-
bioRxiv - Microbiology 2022Quote: ... Lysates were then centrifuged for 1 h (25,000 rpm, Beckman Coulter Avanti J26-XP centrifuge ...
-
bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
-
bioRxiv - Microbiology 2021Quote: ... and stained for 30min at room temperature with 1:160 dilution of PE-conjugated mouse-anti-human Ab to HIV-1 p24 and core antigens (Beckman Coulter, Fullerton, CA). Fluorescent profiles were recorded using a Guava easyCyte and InCyte software.
-
bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
-
bioRxiv - Microbiology 2023Quote: ... PBS-washed cells were PFA-fixed for 90 minutes and immunostained with the following antibodies diluted in 0.1% Triton X-100 in PBS: anti-HIV-1-core-antigen-FITC or -PE (#6604665 or #6604667; Beckman Coulter), rabbit-anti-human-MX1/2 (#sc-166412 ...
-
bioRxiv - Immunology 2023Quote: ... Surface antigen expression was measured with the CytoFLEX LX (Beckman Coulter) and data was analyzed with the CytExpert Software Version 2.4 (Beckman Coulter ...
-
bioRxiv - Biochemistry 2019Quote: ... 15 mM imidazole and lysed by sonication in the presence of 10 μL of 1 U.ml-1 DNase I per litre of over-expression culture and cell lysate was clarified (Beckman JA25.50 rotor ...
-
bioRxiv - Microbiology 2023Quote: ... The lysate was cleared at 100×kg for 1 h in Ti70 rotor (Beckman Coulter). The pellet was subsequently resuspended in lysis buffer with 8 M Urea and 20 mM imidazole at room-temperature for 1 h ...
-
bioRxiv - Neuroscience 2024Quote: ... the cell lysate underwent centrifugation at 12,000 × g for 1 h (JA 25.50, Beckman Coulter) and supernatant was loaded to anti-Flag M2 affinity resin (GenScript) ...
-
bioRxiv - Biophysics 2020Quote: ... Lysate was clarified by ultracentrifugation (Beckman L8-M ultracentrifuge ...
-
bioRxiv - Cancer Biology 2022Quote: ... Antigen cross-presentation was assessed using a Cytoflex S flow cytometer (Beckman Coulter) and was measured as the percentage of APC+ cells within CD45highCD11b+ MdCs and CD45lowCD11b+ MG ...
-
bioRxiv - Immunology 2021Quote: ... The lysate was centrifuged at 42,000 RPM for 1 h (45 Ti fixed-angle rotor, Beckman), and the supernatant was used for affinity chromatography with MBP beads by gravity flow ...
-
bioRxiv - Immunology 2021Quote: ... The lysate was centrifuged at 42,000 RPM for 1 h (45 Ti fixed-angle rotor, Beckman), and the supernatant was used for affinity chromatography with anti-FLAG M2 affinity gel (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysate was clarified by centrifugation at 55,000 g for 1 h in 25.50 rotor (Beckman Coulter). Clarified supernatant was loaded on 1.5 mL of amylose resin (NEB ...
-
bioRxiv - Biochemistry 2023Quote: ... The crude lysate was centrifuged for 1 hour at 20,000 rpm and 4 °C (Beckman Coulter). The supernatant was filtered using a 0.44 μm syringe filter (Sartorius ...
-
bioRxiv - Biophysics 2023Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour by Beckman Optima™ L-80XP ultracentrifuge equipped with a Ti-45 rotor (Beckman ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
-
bioRxiv - Microbiology 2024Quote: ... The virus was enriched by ultracentrifugation (Beckman Coulter Optima™ L-80XP) at 246,347g for 4 hours at 4 °C ...
-
bioRxiv - Immunology 2022Quote: ... The cell lysate was centrifuged at 42,000 rpm for 1 h (45 Ti fixed-angle rotor, Beckman) at 4 °C.
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were ultracentrifuged (Beckman Optima XE-100) at 27000 rpm for 30 minutes at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation (Beckman-Coulter JA-14 rotor ...
-
bioRxiv - Cell Biology 2021Quote: ... The soluble lysate fraction was collected after centrifugation for 1 hour at 16000 rpm (Beckman Coulter JA-25.50) at 4°C ...
-
bioRxiv - Biophysics 2019Quote: ... and the lysate was clarified by centrifugation for 1 h at 192,838 g in a Type 50.2 Ti rotor (Beckman) at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... the lysate transferred into ultracentrifugation tubes (Beckman Coulter). The iodixanol discontinuous gradient (15% ...
-
bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
-
bioRxiv - Synthetic Biology 2020Quote: Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 28) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor) for 2h at 4 °C ...
-
bioRxiv - Neuroscience 2019Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by aspirating the interface between 40-60% iodixanol with a syringe ...
-
bioRxiv - Neuroscience 2022Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by collecting the interface between 40 and 60% iodixanol with a syringe ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
-
bioRxiv - Biochemistry 2020Quote: ... Cell debris was removed from lysates by ultracentrifugation (Beckman Coulter 70-Ti rotor ...
-
bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were lysed and the lysate was clarified by centrifugation for 1 h at 100,000g (45 Ti rotor, Beckman Coulter). The clarified lysate was incubated with Ni2+-NTA agarose resin for 1 h at 4 °C ...
-
bioRxiv - Microbiology 2020Quote: ... The cell lysate was collected and centrifuged at 100,000 x g for 1 h in an ultracentrifuge (Model XL-90, Beckman Coulter). The supernatant was collected ...
-
bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
-
bioRxiv - Molecular Biology 2021Quote: Normalized cell lysates were in polypropylene ultracentrifuge tubes (Beckman; #357448) and spun in a Beckman-Coulter Optima Max-XP ultracentrifuge ...
-
bioRxiv - Biochemistry 2021Quote: ... lysates were then cleared by ultracentrifugation (Type 45 Ti (Beckman), 35 min ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged using an Avanti JXN30 (Beckman Coulter) with a JA30.50 Ti rotor at 28,000 rpm (94,830 ×g) ...
-
bioRxiv - Molecular Biology 2022Quote: ... the lysate was centrifuged in a JLA 16.250 (Beckman Coulter) at 16,000 rpm for 15 min at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... The lysate was ultra-centrifuged (Beckman Coulter Optima L90K 77LR271) at 40,000 x g ...
-
bioRxiv - Biochemistry 2019Quote: ... Cell lysate was centrifuged with a JA25.5 rotor (Beckman Coulter) at 39,000 g for 40min at 4 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... The clarified lysate was transferred to Optiseal tubes (Beckman #361625) containing layered gradients of iodixanol (15% ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was centrifuged in a JLA-16.250 rotor (Beckman) at 16,000 rpm for 15 min at 4 °C ...