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Citations for Beckman :
1 - 50 of 87 citations for GDF 11 BMP 11 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... at 50.000 rpm and 11 °C (Beckman SW65 Ti Rotor). The virion band was extracted using an 18‐ gauge needle syringe ...
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bioRxiv - Cancer Biology 2022Quote: ... a 0.55× volume (11 µL) of SPRIselect (Beckman Coulter, B23317) was added to the purified library ...
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bioRxiv - Biophysics 2020Quote: ... in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman, ref. 343788). Ribosome pellets were washed once and subsequently resuspended with ribosome storage buffer (30 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 M sucrose) in a 11×34 mm polypropylene tube (Beckman Coulter) and centrifuged for 6 hours at 55,000 rpm at 4°C on a TLS-55 rotor (Beckman Coulter ...
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bioRxiv - Biochemistry 2019Quote: ... in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman, ref. 343788). Ribosome pellets were washed once and subsequently resuspended with ribosome storage buffer ...
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bioRxiv - Systems Biology 2020Quote: ... in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778), and the resultant supernatant was ultracentrifuged at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 40 min at 20 000 g (8 °C, 11 300 RPM, Beckman JA-13.1) to run a 30 ml 20% Percoll purification (in 250 mM Sorbitol ...
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bioRxiv - Cell Biology 2023Quote: ... The PMF was loaded in two 11 × 60 mm centrifuge tubes (cat. 344062, Beckman) and then centrifuged on an SW 60 Ti rotor (Beckman ...
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bioRxiv - Biophysics 2024Quote: ... Floatation assay was performed with polycarbonate centrifugation 11×34 mm tubes (Beckman Coulter, USA) and sucrose solutions prepared in HK buffer ...
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bioRxiv - Immunology 2021Quote: ... Purified cDNA was amplified for 11 cycles before being cleaned up using SPRIselect beads (Beckman). Samples were diluted 4:1 (elution buffer (Qiagen):cDNA ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.5) in a 11 x 34 mm Ultra-Clear centrifuge tubes (Beckman Coulter: 347356). The sucrose density gradient was centrifuged at 50,000 rpm for 3 hours at 4°C in a TLS-55 swinging bucket rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of the eluate was transferred into a Beckman 11-mm ultracentrifuge tube (Beckman Coulter) for ultracentrifugation in an Optima TLX ultracentrifuge (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... Purified cDNA was amplified for 11 cycles before being cleaned up using SPRIselect beads (Beckman Coulter). Samples were diluted 9:1 (elution buffer (Qiagen):cDNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 20 min at 20 000 g to pellet mitochondria (8 °C, 11 300 RPM, Beckman JA-13.1), 40 min at 20 000 g (8 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and further centrifuged 20 min at 20 000 g (8 °C, 11 300 RPM, Beckman JA-13.1) to pellet mitochondria after Percoll removal by dilution with 40 mL mitochondrial wash buffer (250 mM Sorbitol ...
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bioRxiv - Genomics 2019Quote: ... we cleaned up single cell RNA by adding 11 µl of RNAClean SPRI beads (Beckman Coulter Genomics) into each well of 5 µl of cell or nucleus lysate ...
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bioRxiv - Pathology 2021Quote: ... 20 U/ml SUPERase·In) in thickwall 11 x 34 mm polycarbonate tubes (Beckman Coulter Life Sciences; 343778). Samples were weighed and balanced by adding PEB and centrifuged in TLA100.3 rotor at 100,000 rpm for 1.4 h in a pre-chilled ultracentrifuge (Beckman coulter Optima MAX-XP Ultracentrifuge) ...
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bioRxiv - Physiology 2019Quote: ... Lysates (≈11 ml) were centrifuged at 26,000xg for 30 min at 4°C in a SW41Ti rotor (Beckman). The floating lipid droplet layers were harvested with a glass pipette and adjusted to 25% sucrose and 100 mM sodium carbonate ...
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bioRxiv - Biophysics 2022Quote: ... We purified the folded chassis by glycerol gradient sedimentation by centrifugation[47] through a 10-45% glycerol gradient in TBE buffer supplemented with 11 mM MgCl2 for 130 min at 242,704g in a SW50.1 rotor (Beckman) at 4°C and collected in fractions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Linear 10–30% glycerol gradients were prepared in 11□mm□×□34□mm polyallomer centrifuge tubes (Beckman Coulter, Brea, CA) with HEM buffer [25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2020Quote: ... and layered onto a continuous 10-50 w/w % sucrose gradient prepared in a 2.2 ml ultra-clear tube (11×34 mm, Beckman Coulter) using a two-step program in Gradient Master 108 machine ...
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bioRxiv - Microbiology 2022Quote: ... and layered onto a preformed 11 mL 5% to 15% (wt/vol) Ficoll gradient in a 13.2 mL thin-wall polyallomer ultracentrifuge tube (Beckman Coulter). Gradients were centrifuged at 12,000 rpm for 2 h at 4°C in an SW41 Ti swinging-bucket rotor in a Sorvall Discovery SE ultracentrifuge ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting cDNA was PCR amplified (11 cycles) and purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics). For Illumina NextSeq sequencing ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.5) at 45,000 RPM for 45 minutes at 11 °C in a Beckman 70.1 Ti rotor (Beckman Coulter, Brea, California). The pellet was resuspended in 10 mM Tris-HCl ...
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bioRxiv - Plant Biology 2022Quote: ... Sucrose step gradient (sucrose in resuspension buffer) were prepared in Ultra-Clear™ centrifuge tube 11×89 mm (Beckman Coulter) starting from the bottom (%w/w) ...
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bioRxiv - Neuroscience 2023Quote: ... The top 11 mL and the bottom 2 mL of the gradient were transferred to separate 60 mL 45Ti ultracentrifuge tubes (Beckman), topped up with PBS and centrifuged at 100,000 x g for 70 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... starting with 50% and topping off with 15% for a total of 11 mL was added to thin wall polypropylene ultracentrifuge tubes (Beckman Coulter). The tubes were immediately frozen and stored at -80 °C ...
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Cell Biology 2023Quote: ... A volume of lysate containing 125 ug RNA was loaded on 11 ml 15-50% sucrose gradients in 14 x 89 mm centrifuge tubes (Beckman Coulter). (Gradients were prepared in 20 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell suspensions were diluted 250 μL into 10 mL of ISOTON diluent and cell size was measured on a Coulter Z2 Cell Analyzer (Cat# 844 80 11, Beckman, Indianapolis, IN). Median cell volume was used for calculations ...
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bioRxiv - Microbiology 2020Quote: ... were further purified by centrifugation through a 1.2 g/cm3 to 1.6 g/cm3 cesium chloride density gradient at 50,000 RPM for 4 hours at 11 °C in a Beckman SW 65 Ti rotor (Beckman Coulter, Brea, California). Visible “virus bands” in the gradients were collected using a Hamilton syringe and diluted 1:10 in 10 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Diluted virions were then centrifuged at 75,000 RPM for 30 minutes at 11 °C in a Beckman TLA 120 rotor (Beckman Coulter, Brea, California). The pellet was resuspended in 10 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... Tie2-PE and CD34-FITC antibodies (eBioscience 17-5821-81, 17-1171, 12-5987, 25-0411, 11-0341-82) by flow cytometry (Beckman Coulter CyAn ADP) or confocal microscopy using Confocal Microscope Zeiss LSM 880 with Airyscan.
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bioRxiv - Microbiology 2022Quote: Whole-cell absorbance spectra from 550 to 800 nm were taken from cells grown in BG-11 under VL and FRL for 30 days using a DU 640 spectrophotometer and associated software (Beckman Coulter, Brea, CA). For low-temperature fluorescence measurements at 77 K ...
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bioRxiv - Systems Biology 2022Quote: ... supernatant containing metabolites extracted from 1.1×109 cells and 11 spike-ins were aliquoted into 1,000 vials using an automated liquid handler (Biomek 4000, Beckman Coulter, Inc., Brea, California, USA), with 5 μL solution per tube ...
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bioRxiv - Bioengineering 2021Quote: ... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
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bioRxiv - Systems Biology 2021Quote: HeLa Kyoto and R1/E mESCs (gifts from Hyman lab, Dresden) and HEK293 (gift from Beckman lab, Berlin), Ptk2 cell lines (gift from Simons lab ...
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bioRxiv - Immunology 2021Quote: Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter). Test sera were diluted 1:40 in growth medium and serially diluted ...
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bioRxiv - Developmental Biology 2023Quote: The anti–human uncoupled primary antibodies used are listed: anti-human CD34 (Beckman, QBEnd/10), anti-human ACE (BB9 ...
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bioRxiv - Immunology 2022Quote: ... anti-human TCR Vβ1 (Beckman Coulter), anti-human CD3 and CD8 antibodies (BD Bioscience).
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bioRxiv - Immunology 2021Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2022Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Immunology 2022Quote: Human Vγ9Vδ2 T lymphocytes were stained with FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and APC-labeled anti-human CD3ε mAb (#UCHT1 ...
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bioRxiv - Bioengineering 2021Quote: ... APC-labelled anti-human CD8 (Beckman Coulter, #IM2469), APC- or PacificBlue-labelled anti-human CD3 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD4-FITC (clone 13B8.2, Beckman Coulter), anti-human CD8a-BV421 (clone RPA-T8 ...
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bioRxiv - Molecular Biology 2021Quote: ... or 13 nM anti-human CD81 PE (Beckman Coulter), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... or 13 nM anti-human CD81 FITC (Beckman Coulter) antibody solution ...
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bioRxiv - Immunology 2022Quote: ... FITC anti-human TCR Vβ17 (Beckman Coulter, cat. IM1234), FITC anti-human TCR Vβ21.3 (Backman Coulter ...
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bioRxiv - Immunology 2022Quote: ... FITC anti-human TCR Vβ7.2 (Beckman Coulter, cat. B06666), and Beta Mark TCR Vbeta Repertoire Kit (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... and FITC-conjugated anti-human CD42a mAb purchased from Beckman Coulter (Brea ...