Labshake search
Citations for Beckman :
1 - 50 of 1673 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2020Quote: ... then resuspended the pellet in 0.1% BSA-PBS containing 100 ng/mL DAPI and proceeded with FACS on a MoFlo Astrios (Beckman Coulter). After gating for singlets and live cells ...
-
bioRxiv - Genetics 2020Quote: ... 99 µl Agencourt RNAclean XP (Beckman Coulter Life Sciences) were added and incubated at RT for 15 min ...
-
bioRxiv - Immunology 2020Quote: ... all CCMV CP preparations were purified two times using an ultracentrifuge and 30% sucrose cushion – first with additional 0.5% Triton X-100 and the second time without Triton X-100 (4 h, 50 000 rmp, 4°C; Type 70Ti rotor, Beckman) and the pellet was then dissolved in buffer A ...
-
bioRxiv - Microbiology 2020Quote: ... of 1.5 mL and then centrifuged 45 min at 100,000g at 4°C in a TLA-100 ultracentrifuge (Beckman Coulter). The supernatant contains the soluble fraction and the pellet corresponds to the total membranes ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.5 mM DTT, 100 μg/ml cycloheximide) and centrifuged (160,000 g, 120 min, 4°C) in an SW41Ti rotor (Beckman). After centrifugation the sediment in the tube went through a UV detector at the wavelength of 254nm and simultaneously the required fractions (>=5 ribosomes ...
-
bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
-
bioRxiv - Genetics 2022Quote: ... with purity > 99% using a MoFlo XDP instrument (Beckman Coulter) (Table S1 ...
-
bioRxiv - Immunology 2023Quote: ... and soluble anti-CD28 (500 ng/ml; Beckman Coulter, REF# IM1376) antibodies in presence of cytokines i.e. ...
-
bioRxiv - Biophysics 2019Quote: ... 4 °C for 10 min using TLA-100 rotor (Beckman-Coulter). Tubulin in the supernatant was polymerized by incubating in the dark at 37 °C for 20 min ...
-
bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 4.5 volumes of ice-cold extraction buffer and re- centrifuged at 11,300 RPM for 10 min at 4°C using a Ti45 rotor (Beckman). The supernatant was filtered through a 70 μm pore size cell-strainer and combined with the first supernatant ...
-
bioRxiv - Microbiology 2023Quote: ... 100 ng of DNA from each sample was buffer exchanged via Ampure XP bead purification (Beckman Coulter Life Sciences product number A63881 ...
-
bioRxiv - Plant Biology 2021Quote: ... 100 μg/mL cycloheximide in 60% sucrose) in a 26 ml polycarbonate tube (Beckman Coulter). After proper balancing ...
-
bioRxiv - Microbiology 2023Quote: ... then the colonies were diluted and inoculated into the Neg MIC EN-2J panel containing β-lactams listed in Table 2 and incubated in MicroScan Walkway40 Plus (Beckman Coulter Inc.). Breakpoint criteria for susceptible (S) ...
-
bioRxiv - Cell Biology 2020Quote: ... 4°C for 1 h with MLS-55 rotor (Beckman Coulter). The pellets were lysed in 40 μL of radioimmunoprecipitation (RIPA ...
-
bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
-
bioRxiv - Cell Biology 2023Quote: ... Samples were spun at 200,000 × g at 4°C for 4 h in an MLS-50 rotor (Beckman Coulter), and 60 µL of top layer was collected ...
-
bioRxiv - Molecular Biology 2019Quote: ... 4 ml of 10 % and 3.5 ml of 5 % iodixanol in a 17 ml Thinwall Polypropylene Tube (Beckman Coulter, Fullerton, California, USA). The DG was centrifuged 18 h at 100,000 g and 4 °C using SW 32.1 Ti rotor (Beckman Coulter ...
-
bioRxiv - Biochemistry 2019Quote: ... After ultra-centrifugation (SW-60Ti rotor Beckman Coulter, 2 h, 100 000 rpm, 4°C) the gradient was manually fractioned in 200 μl fractions from the top to the bottom of the tube ...
-
bioRxiv - Cell Biology 2023Quote: ... Upon centrifugation for 30 min at 4 °C (96,000 g, Rotor TLA-100, Beckman Coulter), the resulting supernatant yields soluble glycosomal matrix proteins while the pellet contains membrane-associated proteins ...
-
bioRxiv - Cell Biology 2022Quote: ... 4–40% sucrose gradient prepared in lysis buffer and centrifuged at 150,000 × g for 4 or 16 h at 4°C in a MLS-50 rotor (Beckman Coulter). Thereafter ...
-
bioRxiv - Biochemistry 2022Quote: ... by dissolving the yeast pellet in 100 mL wash buffer per 100 g cell pellet and centrifugation (JLA 8.1000 rotor, Beckman) at 5,000 rpm ...
-
bioRxiv - Biophysics 2022Quote: ... 100µL samples were then centrifuged at 42000rpm for 30min at 4°C (Beckman TLA 100 rotor). 2 fractions were isolated from these samples ...
-
bioRxiv - Immunology 2023Quote: ... and spun for 100 min at 38,000rpm at 4°C in a SW41Ti rotor (Beckman Ultracentrifuge). Gradients were syringe-fractionated mechanically from the bottom up and monitored for absorbance at 254nm (Teledyne Isco ...
-
bioRxiv - Microbiology 2022Quote: ... Fractions were diluted in 5 ml PBS and washed by ultracentrifugation at 200,000 × g (60 min, 4°C, AH-650 rotor, 5 ml Beckman Ultra-Clear tubes ...
-
bioRxiv - Systems Biology 2023Quote: ... the supernatant was then divided into 4 x 1.5 mL Ultracentrifuge Tubes (Beckman) and processed for differential ultracentrifugation step with an Optima TLX-BenchTop Ultracentrifuge (Beckman ...
-
bioRxiv - Immunology 2021Quote: ... 4 ng of RNA were retrotranscribed and cDNA was amplified using 13 cycles and purified with AMPure XP beads (Beckman Coulter). Concentration was determined using Qubit 3.0 (Life Technologies ...
-
bioRxiv - Immunology 2020Quote: ... and antibodies against membrane markers were added (CD45, CD14, HLA-DR from Beckman Coulter). Intracellular staining was performed using the IntraPrep fixation permeabilization kit (Beckman Coulter ...
-
bioRxiv - Microbiology 2024Quote: ... 0.2% Triton X-100 and 100 µg/mL chloramphenicol) and ultracentrifuged at 42500 rpm for 5 h in type 70 Ti rotor (Beckman Coulter) at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... Following ultra-centrifugation (100,000 x g; 4°C; 15 min; rotor TL-100; OptimaMax-XP, Beckman-Coulter), solubilized membrane protein samples (20μl extract with BN-PAGE loading buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... 086 x g for 2.5 hours at 4°C using Optima L-100 XP ultracentrifuge (Beckman Coulter), and the pellet was resuspended in LHCN medium ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... 100 ng of DNA was sheared to 300 bp using the Covaris LE220 and size selected using SPRI beads (Beckman Coulter). The fragments were treated with end-repair ...
-
bioRxiv - Microbiology 2022Quote: ... 100 µg/mL cycloheximide) in a Beckman 14×89mm tube (Beckman Coulter 331372) and adding 5.5 mL of 15% sucrose solution (42.5 mL 1X LSB (20 mM Tris pH 7.4-7.5 ...
-
bioRxiv - Pathology 2022Quote: Cell death and apoptotic markers were recorded by flow cytometry (FC500, Beckman Coulter, Villepinte, France). Cell viability was analyzed by FDA (Fluorescein di-acetate ...
-
Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... The samples were then centrifuged at 107,163.6 X g for 2.5 hours at 4°C in an ultracentrifuge (XPN 100, Beckman Coulter). After centrifugation ...
-
bioRxiv - Microbiology 2024Quote: ... The eluent was ultracentrifuged (100,000 ×g, 4°C, 16 h) using Optima L-100 XP Ultracentrifuge (Beckman Coulter) to sediment ribosomes.
-
bioRxiv - Cell Biology 2023Quote: ... pH 11.5) and were centrifuged for 60 min at 4 °C (150,000 g, Rotor TLA-100, Beckman Coulter). The yielded pellet was analyzed by immunoblotting and mass spectrometry (Supplementary Figure 1).
-
bioRxiv - Cell Biology 2023Quote: ... pH 11.5) and is centrifuged for 60 min at 4 °C (150,000 g, Rotor TLA-100, Beckman Coulter). The resulting supernatant contains the released matrix proteins and peripheral membrane proteins ...
-
bioRxiv - Cell Biology 2023Quote: ... pH 11.5) and was centrifuged for 60 min at 4 °C (150,000 g, Rotor TLA-100, Beckman Coulter). The resulting supernatant contains matrix proteins and peripheral membrane proteins ...
-
bioRxiv - Immunology 2022Quote: ... αCD21 biotinylated (eBiosciene) and Streptavidin Cy5 (ImmunoResearch) and isolated with a purity >99% with the MoFlo cell sorter (Beckman Coulter). Splenic naïve B cells were isolated by magnetic cell sorting using the “EasySep™ Mouse B Cell Isolation Kit” from STEMCELL according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2022Quote: ... samples (10 mL) were first centrifuged at 7000Xg at 4°C for 30 minutes (Beckman Coulter Avanti J-E Series ...
-
bioRxiv - Microbiology 2023Quote: 50 mL of freshly prepared phage lysate (> 108 pfu/mL) was ultracentrifuged (Optima XE-100 ultracentrifuge, Beckman Coulter, Brea, CA, USA) at 109 ...
-
bioRxiv - Biochemistry 2024Quote: ... The extraction was centrifuged at 39,000 g (Beckman) for 1 hour to remove the insoluble components ...
-
bioRxiv - Biochemistry 2023Quote: ... The extraction was centrifuged at 39,000 × g (Beckman) for 60 minutes to remove insoluble components ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 600 ng of pool amplicons in 100 µl of volume were cleaned up with 1.0X volume of AMPureXP (Beckman-Coulter, West Sacramento, CA) beads according to the manufacturer’s directions ...
-
bioRxiv - Molecular Biology 2019Quote: ... The 80S fraction was pelleted for 1 h at 436000 x g at 4 °C (TLA-100, Beckman Coulter), resuspended in 20 mM HEPES pH 7.4 ...
-
bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 10,000g and 4°C for 30 minutes (SW41 rotor, Optima XPN-100 ultracentrifuge; Beckman Coulter, Inc.) to remove any potential large debris ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman). Supernatants were kept aside as “Supernatant” fraction ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 82,600×g at 4°C for 2 h (Beckman ultracentrifuge optima L-100 XP). After the first ultracentrifugation ...
-
bioRxiv - Cell Biology 2023Quote: ... 20µM ATP and 1 mM DTT and subsequently sedimented (100,000 x g, 15 min, 4°C, TLA-100 rotor) in an Ultima MAX-XP ultracentrifuge (Beckman). For competition assays ...
-
bioRxiv - Neuroscience 2024Quote: ... and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...