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Citations for Beckman :
1 - 50 of 57 citations for Elabela 19 32 TFA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... in a swing rotor (SW-32-Ti; Beckman). A single orange band in the upper third of the gradient ...
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bioRxiv - Biophysics 2021Quote: ... and analyzed in Expo 32 software (Beckman Coulter).
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bioRxiv - Microbiology 2020Quote: ... and centrifuged at 100,000 × g (Beckman SW 32 Ti rotor) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... equipped with a SW 32 Ti swinging bucket (369650, Beckman Coulter). Supernatants were discarded by inverting tubes ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were purified using 32 μL Ampure XP beads (Beckman Coulter, A63881) and eluted in 12 μL NF H2O ...
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bioRxiv - Cell Biology 2023Quote: ... followed by centrifuge at 95,000g on a SW 32 Ti rotor (Beckman) for 1 h at 4 °C to obtain the pure MAM (the pellets).
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bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was centrifuged at 12,000rpm in a SW-32 rotor (Beckman Coulter) for 15min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Samples from COVID-19 patients and controls were assessed on a Cytoflex LX cytometer (Beckman Coulter), verified through daily calibration with CytoFLEX Daily QC Fluorospheres (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2020Quote: ... Gradients were centrifuged at 20,755 × g in an SW 32 Ti rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS inside a 32 mL open-top thickwall polycarbonate ultracentrifuge tube (Beckman Coulter) and centrifuged using SW32 Ti rotor in Optima XE-90 ultracentrifuge (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... 4°C for 2 h (SW 32 Ti swinging-bucket rotor, Optima LE-80K Beckman Coulter) using a 25% w/v sucrose cushion ...
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bioRxiv - Genomics 2021Quote: ... up to 32 single cell reactions were pooled and 1.2x SPRI bead purified (AMPureX beads, Beckman Coulter). The DNA was eluted in 10 µl of 10 mM Tris-Cl (pH8.5 ...
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bioRxiv - Genetics 2019Quote: ... Libraries were amplified again for an additional 17-19 cycles and left side size selected with SPRIselect beads (Beckman Coulter) at a 1:1 ratio following manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Extracellular HSV-1 or HSV-2 particles were harvested by sedimentation at 12,000 rpm for 90 minutes at 4°C (Type 19 rotor, Beckman-Coulter) from the medium of BHK-21 cells (40 x 175 cm² flasks ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Ribosomes were pelleted by centrifugation at 63,000g for 16–19 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). For all ultracentrifugation steps indicated here ...
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bioRxiv - Microbiology 2022Quote: ... at 20 KPSI at 4°C and then centrifuged at 19 000 RPM in a JA-25.50 rotor (Beckman Coulter) for 30 minutes at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Gradients were centrifuged at 28,000 rpm (97,000 xg) for 16 hours in a SW-32 rotor (Beckman-Coulter). An ISCO gradient fraction system was used to isolate the 70S fraction (Figure S13).
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged through a 10–35% sucrose gradient for 19 h at 16,000 rpm in a SW28 rotor (Beckman Coulter, Inc.). Fractions containing the 70S peaks were pooled and kept at −80℃ for further use.
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bioRxiv - Cell Biology 2020Quote: ... Crude EVs were obtained by centrifugation at 20,000 g (30 minutes) or 100,000 g (70 minutes) (Optima XPN Ultracentrifuge, SW 32 Ti Rotor, Beckman Coulter) for large and/or small populations ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was than applied on a glycerol step gradient (30-12% glycerol) and centrifuged for 15 h at 25,000 rpm (SW 32 rotor, Beckman). The separation and distribution of proteins was confirmed by SDS-PAGE gel (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels ...
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bioRxiv - Plant Biology 2020Quote: ... and centrifuged at 131,500 × g for 30 min at 4 °C using a SW 32 Ti rotor (Beckman Coulter). Thylakoid membranes sedimented in 1.3 M sucrose cushion were collected and washed with 25 mM Tricine-KOH (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... Viral particles were pelleted at 30,000 rpm for 1.5 h at 25°C in an Optima XPN ultracentrifuge using an SW 32 Ti rotor (Beckman Coulter), resuspended in 500 μL DMEM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... with 2 ml of 20% sucrose in dPBS cushion and ultracentrifugated at 20,000 RPM (Beckman Coulter SW 32 Ti rotor) at 4°C for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25 % sucrose (dissolved in H2O) was overlaid with filtered supernatant and centrifuged in a swing-out rotor ((SW 32 Ti; Beckman Coulter or SureSpin™ 630/36 ...
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bioRxiv - Genomics 2021Quote: ... lentiviral supernatant was ultracentrifuged at 23,000 RPM at 4 °C for 2 hours in an SW 32 Ti swinging bucket rotor (Beckman Coulter). After centrifugation ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... FCS was diluted 1:1 with RPMI and centrifuged at 100,000 g at 4 °C in a swinging-bucket rotor (SW28 or 32, Beckman Coulter) for 18 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Ultra-centrifugation was performed (160,000 x g; 16 hours; 4°C; SW 32 Ti rotor; Optima XPN-80, Beckman Coulter) and 1mL fractions were collected and analyzed by BN-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... purified by ultracentrifugation through a sucrose cushion (25% weight/volume in Tris-NaCl-EDTA buffer) for 75 min at 4°C and 28,000 rpm using a SW 32 TI rotor (Beckman Coulter), resuspended in serum-free RPMI 1640 or DMEM medium and stored in small aliquots at −80°C ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
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bioRxiv - Microbiology 2022Quote: ... purified by ultracentrifugation through a sucrose cushion (25% weight/volume in Tris-NaCl-EDTA buffer) for 75 min at 4°C and 28,000 rpm using a SW 32 TI rotor (Beckman Coulter), resuspended in serum-free DMEM medium and stored in small aliquots at −80°C ...
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bioRxiv - Biochemistry 2023Quote: ... made in TEMB buffer) and ultracentrifuged at 105,000 x g for 35 min (SW 32 Ti Swinging-bucket, Beckman Coulter). Gradients were fractionated ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysed cells were centrifuged and the viscous supernatant was immediately loaded on a 5-24% (w/v) sucrose density gradient and centrifuged in SW 32 Ti rotor (Beckman) overnight ...
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bioRxiv - Immunology 2024Quote: ... and nanocontainers were then further purified by sucrose density ultracentrifugation (10-40% w/v gradients prepared in 50 mM potassium phosphate buffer at pH 7.5) in a SW-32 rotor (Beckman Coulter) spinning at 28,000 rpm for 4 hours at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... 150 μl of the sample was ultracentrifuged at 22°C and 205000 × g for 19 min (TLA 100.3, Beckman Coulter Inc., Fullerton, California). The experiments were performed in HEPES buffer ...
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bioRxiv - Neuroscience 2019Quote: ... with 2 ml of a 20% sucrose in dPBS cushion and ultracentrifugated at 20,000 RPM (Beckman Coulter SW 32 Ti rotor) at 4°C for 2 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared supernatant was layered over 15-40% sucrose gradients in disassociation buffer and spun 28000 rpm for 16 hours at 4°C in a SW-32 rotor (Beckman-Coulter). Gradients were fractionated with an ISCO fractionation system and 30S + 50S peaks were combined ...
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bioRxiv - Biochemistry 2019Quote: ... The resulting supernatant was loaded onto a 32% sucrose cushion prepared in Buffer A and centrifuged at 28,000 rpm (Beckman SW41Ti rotor) for 16 h ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was then collected and subjected to ultracentrifugation at 100,000 x g for 70 min at 4°C using an Optima XPN-80 ultracentrifuge with SW 32 Ti swinging rotor (Beckman Coulter). Pellets were washed in PBS 1X and EVs were subjected to an additional centrifugation at 100,000 x g for 30 min at 4°C using an Optima MAX ultracentrifuge ...
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bioRxiv - Neuroscience 2020Quote: ... centrifuged at 100,000 g for 70 min at 4°C using an Optima XPN-80 ultracentrifuge with SW 32 Ti swinging rotor (Beckman Coulter) and then processed for further analyses.
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bioRxiv - Plant Biology 2022Quote: ... Thylakoid membranes were extracted via ultracentrifugation at 24,000 rpm for 1 h and 20 min (Beckman Coulter SW 32 Ti rotor). Thylakoids were collected from the step gradient interphases with a Pasteur pipet ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 4°C at 31,000 rpm for 2 h and 30 min using an SW 32 Ti rotor(Beckman Coulter, 369694). The gradients were fractionated using a Teledyne ISCO Density Gradient Fractionation System and 12 fractions were collected ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were filled into 38 mL open-top polypropylene tubes and subjected to DUC using a SW Type 32 Ti swinging bucket rotor (Beckman Coulter) in an Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... mtEFG1 was subsequently removed from the mitoribosomes via a 10 – 40 % (w/v) sucrose gradient in 1x MB (17 h, 22500 rpm, SW 32 Ti rotor (Beckman Coulter), 4 °C) ...
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bioRxiv - Cell Biology 2022Quote: ... A 12 ml 2 % step Optiprep gradient in LB ranging from 32 % to 10 % was prepared beforehand in Ultra-Clear tubes (Beckman Coulter). Samples were spun for 16 hours at 100000 g at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... the 30S and 50S subunits were separated on a 15-35% (w/v) sucrose gradient in dissociation buffer on a SW-32 rotor (Beckman-Coulter) spun at 28,000 rpm (97,000 xg ...
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bioRxiv - Cancer Biology 2023Quote: ... and concentrated by ultracentrifugation in V-bottom polypropylene centrifuge tubes on a SW 32 Ti in an Optima XE 90 (Beckman Coulter) at a speed of 22,000 rpm at 4°C for 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 rcf for 70 min at 4 °C using a Optima XE-90 ultracentrifuge with a SW 32 Ti rotor (Beckman Coulter). The EV pellet was resuspended in 0.22 μm-filtered sterile PBS and freshly-used or stored at −80 °C until further use ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation of IL-32 KO and WT mock cells was assessed by cell counting every day for 4 days using cell coulter (Beckman Coulter Diagnostics). Cell viability was assessed by Trypan blue staining or by annexin/PI flow cytometry and analyzed by FACS Diva software (BD Biosciences).