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Citations for Beckman :
1 - 50 of 2555 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... The dissected tissues were held in a glass-bottom dish (35 × 10 mm, IWAKI #3910-035) with an insect pin (φ0.10 mm, Ento Sphinx Insect Pins) and silicone grease (Beckman), and 20 μL of SDM was added to cover the tissue ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 24 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 18 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
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bioRxiv - Microbiology 2022Quote: ... at 4°C cells were resuspended in Isoflow (Beckman) for further reading the percentage of fluorescent labelled cells on the cytometer (FC500 ...
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bioRxiv - Microbiology 2024Quote: ... The virus was enriched by ultracentrifugation (Beckman Coulter Optima™ L-80XP) at 246,347g for 4 hours at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Volumes of single cells were measured using Multisizer 4 Instrument (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were acquired on a 4 laser CytoFlex S (Beckman Coulter) and analyzed using FlowJo software (V10.8.0).
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bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2020Quote: Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 28) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor) for 2h at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... At OD600 1.2 the cells were pelleted at 4 °C (TLA10.500 (Beckman), 15 min at 5,000-8,000 rcf) ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were harvested by centrifugation (Beckman JA10 rotor, 5000rpm, 5min, 4°C) and resuspended in 50 ml buffer A (20 mM HEPES/NaOH ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell count was acquired using a Multisizer 4 Coulter Counter (Beckman-Coulter) using standard protocols and a threshold of 12 to 30 μm.
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bioRxiv - Cell Biology 2024Quote: Cell volumes were measured using a Coulter counter (Multisizer 4, Beckman Coulter), which is based on the principle that a cell traversing an orifice filled with electrolyte will produce an impedance change proportional to the volume of the cell due to displacement of electrolyte ...
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bioRxiv - Microbiology 2023Quote: ... The denatured protein fraction was then removed via ultracentrifugation at 100,000 g for 20 min at 4°C (Beckman Coulter Optima XE-90 – IVD ultracentrifuge with a 42.2 Ti rotor) ...
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bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... and cell size and cell density were measured using the Multisizer 4 Coulter Counter (Beckman-Coulter, Mississauga, ON) or collected for whole cell lysis at time points indicated in the figures ...
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bioRxiv - Biochemistry 2020Quote: ... Protein-liposome complexes were separated from unbound protein by centrifugation (2 h at 45.000 rpm, 4 °C, MLS50 rotor -Beckman Coulter) in the density gradient ...
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bioRxiv - Biochemistry 2020Quote: ... cells were harvested by centrifugation (6000 rpm, JA10, 20 min, 4 °C, Beckman). The pellet was re-suspended in 15-20 ml of 50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were collected after 4 days and counted by Z2 Coulter Counter (Beckman). For cell proliferation assays with fold change in cell number ...
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bioRxiv - Microbiology 2024Quote: ... The cell volume was analysed with a Multisizer 4 Coulter Counter (Beckman-Coulter). Runs were completed in the volumetric mode (100 μL) ...
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bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation of IL-32 KO and WT mock cells was assessed by cell counting every day for 4 days using cell coulter (Beckman Coulter Diagnostics). Cell viability was assessed by Trypan blue staining or by annexin/PI flow cytometry and analyzed by FACS Diva software (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... After cell debris and undisrupted cells were removed by centrifugation (45,000 rpm, 30 min, 4°C, 70Ti rotor; Beckman), supernatant was applied to an open column with Ni-IMAC resin (BIO-RAD) ...
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bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... were pre-clarified to remove aggregate proteins by spinning at 65,000 rpm for 30 min at 4°C in a TLA100 rotor (Beckman Coulter, Brea, CA) before incubating with F-actin ...
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bioRxiv - Cell Biology 2019Quote: ... The obtained supernatant (containing membrane and cytosolic proteins) was then centrifuged at 180,000 × g for 90 min at 4°C using a TLA-55 rotor (Beckman Coulter, Brea, CA). The obtained pellet (containing intact membranes ...
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bioRxiv - Systems Biology 2021Quote: ... Cells expressing the DsRed fluorescent protein were FAC sorted with a MoFlo cell sorter (Beckman Coulter GmbH, Krefeld, Germany) to obtain a highly enriched fraction for neuronal cells.
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bioRxiv - Biochemistry 2021Quote: ... The virus-containing filtrate was then further purified using an ultracentrifuge (Optima XPN-90 Ultracentrifuge, Beckman Coulter, USA) with a cycle of 1,000 rpm (116 g ...
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bioRxiv - Biochemistry 2020Quote: ... the filtered cell culture fluid was ultracentrifuged (25k rpm, 4 °C, SW55Ti Rotor, Beckman Coulter) through a 20% sucrose cushion prepared in TNE buffer (10 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation (Beckman Avanti, 45 min, 40,000 x g, 4 °C) and supernatants were diluted 3-fold in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... Resuspended cells were lysed by French Press (8,000 psi, 4°C, 2 passages) and lysates were centrifuged (Beckman 60Ti rotor, 30 min, 50,000 rpm, 4°C). Pellets were resuspended in 100 ml of buffer B (PBS [140 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The virus was then purified over a 20% sucrose cushion by centrifugation at 35,000 rpm (Beckman Coulter SW41 rotor) for 3 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Virus particles in the supernatant were pelleted by ultracentrifugation at 45.000 rpm for 45 min (Beckman 70.1 Ti Rotor) over a pad of 15% sucrose in 10 mM Tris-HCl buffer (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... Virus RNA was extracted with a magnetic bead-based RNA extraction kit (RNAdvance Viral Reagent kit, Beckman Coulter, C63510) on an epMotion® 5073t liquid handler (Eppendorf ...
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bioRxiv - Plant Biology 2021Quote: ... reinhardtii cells were collected at 1,870 × g and 4°C for 5 min (JLA9.1000, Beckman Coulter). The cells were resuspended at 0.125 mg Chl/mL with disruption buffer containing 25 mM MES-NaOH (pH 6.5) ...
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An oscillating MinD protein determines the cellular positioning of the motility machinery in archaeabioRxiv - Microbiology 2020Quote: The cell volume was analyzed with a Multisizer 4 Coulter Counter (Beckman Coulter, Brea, California, USA) equipped with a 30 nm aperture tube ...
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bioRxiv - Biochemistry 2022Quote: ... Cells debris were pelleted by centrifugation (46,000 x g, 30 min, 4°C, Beckman 45Ti rotor). The supernatant was loaded overnight (in a closed circuit on a peristaltic pump ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed with 4% PFA-PBS and analyzed using a CytoFLEX flow cytometer (Beckman Coulter). ADCP assays were performed in two independent experiments ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris was removed by centrifugation (Beckman JA-25.50, 48,000 x g, 4°C, 30 min), and the supernatant loaded onto a freshly charged 5 mL HisTrap FF Ni-affinity column (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: ... Cell densities in the cultures were determined using a Multisizer 4 Coulter counter (Beckman, Brea, U.S.A).
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bioRxiv - Biophysics 2023Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour by Beckman Optima™ L-80XP ultracentrifuge equipped with a Ti-45 rotor (Beckman ...
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bioRxiv - Bioengineering 2023Quote: ... green fluorescent protein (GFP)-positive cells were FACS-sorted (MoFlo Astrios, Beckman Coulter, California, USA) as single cells into a 96-well plate and clonally propagated ...
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bioRxiv - Biochemistry 2023Quote: ... expression from the viral backbone and cells harvested between 72h-84h after cell proliferation arrest by centrifugation at 1200g for 15min at 4°C (Beckman Coulter centrifuge, JLA8.1000). Further ...
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bioRxiv - Biophysics 2023Quote: ... Virus-containing supernatants were collected at 48 hours and 72 hours after transfection and concentrated using ultracentrifugation (Ultraclear, Beckman 344058). The transduction capacity of the viral vector product was confirmed by culturing non-transfected HEK293T cells with the virus after 24 hours and assessing via fluorescent imaging.
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bioRxiv - Biochemistry 2020Quote: The cell lysates obtained after sonication were centrifuged (16000 rpm, JA-17, 45 min, 4 °C, Beckman) to remove unlysed cells and other high molecular weight debris ...
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bioRxiv - Cancer Biology 2021Quote: ... and cell diameters determined using a particle sizing and counting analyser (Multisizer 4 Coulter counter, Beckman Coulter) with Multisizer 4 software.