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Citations for Beckman :
1 - 50 of 179 citations for Complement Factor B CFB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The immunoturbidimetric test was used to assess serum complement (C) and immunoglobulin antibodies (AU5421; Beckman Coulter, USA). Immunoblotting and immunofluorescence were used to detect serum autoantibodies ...
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bioRxiv - Cell Biology 2024Quote: ... k factor 81.3 (Beckman Coulter). RNA was isolated from the pelleted samples with a miRNeasy micro kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... Centrifugations at 10,000 g (k-factor = 2547.2) and 100,000 g (k-factor = 254.7) were done at +4°C using polyallomer tubes (Beckman Coulter Inc. ...
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bioRxiv - Cancer Biology 2019Quote: ... CD10 PE-TR (B-Ly6; Beckman Coulter; www.beckmancoulter.com); CD14 FITC (M5E2) ...
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bioRxiv - Immunology 2020Quote: ... a lyse-wash protocol (Optilyse B, Beckman-Coulter) was performed ...
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bioRxiv - Cell Biology 2020Quote: ... EVs were ultracentrifuged (Type 45 Ti rotor, k-Factor 133, Beckman Coulter) with 100000 g for 90 min 4°C ...
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bioRxiv - Immunology 2020Quote: ... CD103 (APC, B-Ly7, eBioscience, or biotin, 2G5, Beckman Coulter ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II ...
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bioRxiv - Immunology 2023Quote: ... and B cells were determined using Flow-Count fluorospheres (Beckman) and a Cytoflex flow cytometer (Beckman ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... fixed angle Type 45 Ti rotor (k factor = 133; Beckman Coulter, Miami, FL, USA), at 4°C ...
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bioRxiv - Immunology 2021Quote: ... live B cells were sorted on an Astrios EQ (BeckMan Coulter) into PBS containing 30% FBS (Supplementary Data 2) ...
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bioRxiv - Immunology 2021Quote: ... Stained B cells were analyzed using a Navios cytometer (Beckman coulter). The phenotype of the cells was analyzed using Kaluza Flow Cytometry Analysis and FlowJo software.
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bioRxiv - Synthetic Biology 2023Quote: ... or a CytoFLEX-S (V-B-Y-R) instrument (Beckman Coulter), according to the manufacturers’ protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... Inhibin B was quantified through an enzyme-linked immunosorbent assay (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... while human B cells were CD38+/- (Beckman Coulter #6699531; 1µl per test) and CD19 (BD #557791 ...
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bioRxiv - Physiology 2023Quote: ... apolipoprotein B (apoB) were measured using immuno-turbidimetric assays (Beckman Coulter (UK), Ltd) ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was centrifuged at 10,500 rpm (∼20,000 × g, k factor = 1,753) for 70 min (SW28 rotor, Beckman). Pelleted lEVs were resuspended in 13.5 ml of PBS (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... and sedimented again at 10,500 rpm (∼20,000 × g, k factor = 1,983) for 70 min (SW40 rotor, Beckman). The supernatant containing sEVs was centrifuged at 25,000 rpm (∼ 110,000 × g ...
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bioRxiv - Cancer Biology 2024Quote: ... and the FOXP3/Transcription Factor Staining Kit (eBioscience) and run on the CytoFlex S cytometer (Beckman Coulter). Analysis was performed in FlowJo.
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bioRxiv - Immunology 2021Quote: ... FITC+ APC+ probe-specific B cells were sorted using MoFloAstrios EQ (Beckman Coulter). For bulk sorting ...
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bioRxiv - Microbiology 2020Quote: ... FITC+APC+ Env-specific B cells were sorted using MoFloAstrios EQ (Beckman Coulter). Rabbit PBMCs obtained 30 days after the last injection were prepared for sorting ...
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bioRxiv - Genomics 2020Quote: ... B cells and plasma cells were enriched on a MoFlo Astrio (Beckman Coulter) by excluding duplets ...
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bioRxiv - Genomics 2020Quote: ... The round B product is purified using Agencourt AMPure XP beads (Beckman Coulter).
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed and lysed in 100 µL of OptiLyse B (Beckman Coulter) for 10 min at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... Gradients were centrifuged 18-20h at 192,000g in a SW40 rotor (k-factor 144.5) (Beckman Coulter, Brea, CA). For RNA extraction and small RNA library preparation ...
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bioRxiv - Cell Biology 2021Quote: ... 100K EVs were isolated by ultracentrifugation of 10,000xg supernatant at 100,000xg for 65 min in a SW40 rotor (k-factor 280.3) (Beckman-Coulter). EV-containing 100K ultracentrifugation pellets were resuspended in 20 μL PBS containing 0.2% BSA (cleared from aggregates by ultracentrifugation for 16-20 hrs at 100,000xg) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
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bioRxiv - Cancer Biology 2020Quote: ... The solutions were analysed by flow cytometry on a Cyan B FACS analyser (Beckman Coulter).
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bioRxiv - Microbiology 2020Quote: ... FITC−Dylight 405+APC+ Env-specific B cells were sorted using MoFloAstrios EQ (Beckman Coulter). For bulk sorting ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... NK and NKT or B cells populations were evaluated via flow cytometry (GALLIOS, Beckman Coulter).
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bioRxiv - Synthetic Biology 2023Quote: ... and measurements were conducted using a CytoFLEX-S (V-B-Y-R) instrument (Beckman Coulter) by analyzing 80 µL of each replicate ...
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bioRxiv - Molecular Biology 2020Quote: ... columns were spun at 100.000 g for 24 hours using a SW-28.1 rotor (k-factor: 276) in an optima XPN-80 ultracentrifuge (Beckman coulter), and subsequently fractions of 1 mL were collected ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
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bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
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bioRxiv - Cell Biology 2020Quote: ... The 1 ml concentrates were overlaid on top of the gradient, and centrifuged at 100 000 g (28 000 rpm, k-factor 266) in an Optima L-90K ultracentrifuge (Beckman Coulter) using an SW41 Ti rotor for 18 h at 4 °C with slow deacceleration ...
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were ultracentrifuged at 180,000 × g (38,000 rpm) in a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter, k-factor 145) for 2 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: Single cell RNA was purified from individual B cells using magnetic beads (Beckman Counter, RNAClean XP, A63987) and used for cDNA synthesis by reverse transcription (SuperScript III Reverse Transcriptase ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II, respectively); and βHCG and AFP CMIA-ARCHTECT I 4000.The assays were performed as recommended by the manufacturers ...
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bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... Virus was concentrated from cell-free culture supernatant by high-speed ultracentrifugation through a 30% sucrose cushion at 80,000xg for 16 hrs in a SW32 rotor (k-factor 321) (Beckman Coulter, Brea, CA).
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bioRxiv - Neuroscience 2023Quote: ... the samples were concentrated to a final volume of 240 µl through ultracentrifugation at 42,300 rpm using a Beckman TLA-55 rotor (RCFavg: 80,160; RCFmax: 110,220; k-factor: 110 and the Optima MAX-XP ultracentrifuge (both Beckman Coulter, Brea, CA, USA) and equal sample volumes were immediately used for the EV Neuro panel A and B (115±25 µl ...
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bioRxiv - Cell Biology 2024Quote: ... while the supernatant was further centrifuged at 118.000 × g (38,800 rpm) (Type Ti 45 fixed-angle rotor, Beckman Coulter, k-factor 178) for 2.5 hours at 4°C to isolate small EVs that were resuspended in PBS and stored at –80°C.
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bioRxiv - Microbiology 2020Quote: EV-depleted medium was obtained by overnight ultracentrifugation of DMEM supplemented with 20% FBS at 100,000xg in a Type 45 Ti rotor (Beckman Coulter, K-factor 1042.2). After ultracentrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 45,000 rpm for 16 hours at 4 °C using an Optima™ XPN-80 centrifuge with SW60 Ti rotor (average RCF of 207,976 ×g, maximum RCF of 272,840 ×g, κ-factor of 81; Beckman-Coulter, Fullerton, United States). After centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... and centrifuged at 16,500 × g (14,500 rpm) at 4°C for 20 min using a Type Ti 45 fixed-angle rotor (Beckman Coulter, k-factor 1.275) to isolate large EVs ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to an ultracentrifuge tube and overlayed with layers of Lysis Buffer A with 12% Ficoll and Buffer B with 8% Ficoll (AAB2209518, Fisher) and centrifuged at 28,500 rpm for 45 min (Beckman 42.1). Afterwards ...
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bioRxiv - Microbiology 2020Quote: We stained and sorted single memory B cells as previously described29 using a MoFlo Astrios EQ Cell Sorter (Beckman Coulter). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Ramos B cells were double-stained with Alexa 488-cojugated Nef and anti-CD19-PC-5.5 (J3-119, Beckman Coulter), and interacting infected cell-invaded B cell doublet images acquired with ImageStream X MKII (Amnis/Merck Millipore ...