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Citations for Beckman :
1 - 50 of 669 citations for Aminomethylphosphonic Acid 13C 99%;15N 98%;Methylene D2 98% 100 Ug Ml H2O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... CD4+ or CD8+ CART19 cells were sorted up to 98% purity using the MoFlo Astrios sorter (Beckman Coulter, USA). Likewise ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ug/ml cycloheximide) and macromolecular complexes were separated by velocity gradient ultracentrifugation (SW41Ti – Beckman, 39,000 rpm, 2 hr, 4oC). Fractions were collected using Biocomp Gradient Station and pooled to obtain separate monosomal and polysomal fractions ...
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bioRxiv - Genetics 2020Quote: ... 99 µl Agencourt RNAclean XP (Beckman Coulter Life Sciences) were added and incubated at RT for 15 min ...
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bioRxiv - Genetics 2022Quote: ... with purity > 99% using a MoFlo XDP instrument (Beckman Coulter) (Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... A volume of lysate containing 125 ug RNA was loaded on 11 ml 15-50% sucrose gradients in 14 x 89 mm centrifuge tubes (Beckman Coulter). (Gradients were prepared in 20 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Plant Biology 2021Quote: ... 100 μg/mL cycloheximide in 60% sucrose) in a 26 ml polycarbonate tube (Beckman Coulter). After proper balancing ...
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bioRxiv - Molecular Biology 2023Quote: ... Transduced MOLM-13C cells containing roughly 30% mCherry positive cells were sorted and collected using a FACS MoFlo Astrios Cell sorter (Beckman).
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bioRxiv - Biochemistry 2022Quote: ... by dissolving the yeast pellet in 100 mL wash buffer per 100 g cell pellet and centrifugation (JLA 8.1000 rotor, Beckman) at 5,000 rpm ...
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bioRxiv - Microbiology 2024Quote: ... 0.2% Triton X-100 and 100 µg/mL chloramphenicol) and ultracentrifuged at 42500 rpm for 5 h in type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 100 µg/mL cycloheximide) in a Beckman 14×89mm tube (Beckman Coulter 331372) and adding 5.5 mL of 15% sucrose solution (42.5 mL 1X LSB (20 mM Tris pH 7.4-7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25 % sucrose (dissolved in H2O) was overlaid with filtered supernatant and centrifuged in a swing-out rotor ((SW 32 Ti; Beckman Coulter or SureSpin™ 630/36 ...
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bioRxiv - Immunology 2022Quote: ... αCD21 biotinylated (eBiosciene) and Streptavidin Cy5 (ImmunoResearch) and isolated with a purity >99% with the MoFlo cell sorter (Beckman Coulter). Splenic naïve B cells were isolated by magnetic cell sorting using the “EasySep™ Mouse B Cell Isolation Kit” from STEMCELL according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: 50 mL of freshly prepared phage lysate (> 108 pfu/mL) was ultracentrifuged (Optima XE-100 ultracentrifuge, Beckman Coulter, Brea, CA, USA) at 109 ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction volume was adjusted with H2O to 40 μL and then purified with 72 μL AMPure XP beads (Beckman Coulter), eluting into 20 μL of H2O.
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bioRxiv - Cell Biology 2021Quote: 1 mL samples of culture were diluted 1:100 with Isoton II Diluent (Beckman Coulter) and measured on a Beckman Multisizer 3 Coulter Counter to produce a histogram of the population’s cell volumes ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μg/ml CHX) in nuclease-free water and centrifuged in a SW41Ti rotor (Beckman) for 2.5 hr at 40,000 rpm at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Sample volumes were made up to 20uL with H2O and cleanup was performed using 1.8X using AMPure XP cleanup beads (Beckman Coulter, Cat. A63881) following manufacturer protocol with the exception that samples were incubated for 10mins at room temperature followed by a single wash step using 60uL 80% EtOH and eluted 29uL of elution buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... the labeled oligonucleotide was eluted in 40 to 60 µL H2O and quantitated against a [ψ-32P]-ATP standard curve using a LS 6500 scintillation counter (Beckman-Coulter).
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bioRxiv - Biochemistry 2020Quote: ... 100 µg/ml CHX) in nuclease-free water and centrifuged in a SW41Ti rotor (Beckman Coulter) for 2.5 hours at 40,000 rpm at 4°C ...
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bioRxiv - Cell Biology 2021Quote: 1 mL samples of culture were diluted 1:100 diluted with Isoton II Diluent (Beckman Coulter) and used to determine cell concentration of the culture using a Beckman Multisizer 3 Coulter Counter ...
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bioRxiv - Microbiology 2020Quote: ... The VLP was purified through a double-cushion of 20% and 60% sucrose in H2O in an SW-41 swing-out rotor by ultracentrifugation (Beckman Coulter, Brea, CA) for 2 hours at 25,000 rpm at 4 °C in a swing-out rotor ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was transferred to a polycarbonate ultracentrifuge tube and centrifuged at 350 kg for 30 min (TL-100; Beckman Coulter, TLA-100 rotor (20 × 0.2 mL) to isolate the cytosolic fraction ...
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bioRxiv - Cell Biology 2021Quote: ... and then 100-150 µL was diluted into 10 mL of Isoton II diluent (Beckman Coulter #8546719), and 40,000-50,000 cells were measured per sample ...
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bioRxiv - Biochemistry 2021Quote: ... The UV-Visible spectrum of each sample was recorded for both tannic acid and gallic acid for both pH’s using a DU730 UV-Visible Spectrometer (Beckman Coulter, Pasadena, CA). The spectra were recorded from 250-400 nm with 0.2 nm data intervals ...
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bioRxiv - Microbiology 2020Quote: ... of 1.5 mL and then centrifuged 45 min at 100,000g at 4°C in a TLA-100 ultracentrifuge (Beckman Coulter). The supernatant contains the soluble fraction and the pellet corresponds to the total membranes ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 mM DTT, 100 μg/ml cycloheximide) and centrifuged (160,000 g, 120 min, 4°C) in an SW41Ti rotor (Beckman). After centrifugation the sediment in the tube went through a UV detector at the wavelength of 254nm and simultaneously the required fractions (>=5 ribosomes ...
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bioRxiv - Plant Biology 2021Quote: ... The sonicated pollen samples were filtered through 200 µm sieves using 100 ml Coulter Isoton II diluent (Beckman Coulter) to eliminate anther debris ...
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bioRxiv - Microbiology 2022Quote: ... 1 mM dithiothreitol and 100 μg/ml cycloheximide) and spun for 1 h at 100,000 rpm using TLA-110 rotor (Beckman) at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg/mL Bacitracin) underlay was gently pipetted under the supernatant in polyallomer ultracentrifuge tubes (Beckman-Coulter, Cat #: 326823). The virus was ultracentrifuge concentrated using a SW32 Ti swinging bucket rotor 24,000 rpm for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
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bioRxiv - Systems Biology 2020Quote: ... then resuspended the pellet in 0.1% BSA-PBS containing 100 ng/mL DAPI and proceeded with FACS on a MoFlo Astrios (Beckman Coulter). After gating for singlets and live cells ...
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bioRxiv - Microbiology 2022Quote: A CsCl step gradient was prepared by underlaying 30 mL benzonase treated and clarified lysate with 3 mL 1.2 g/L CsCl solution and 3 mL 1.4 g/L CsCl solution made in 30 mM Tris and 100 mM NaCl (TN) buffer in 38.6 mL Ultra-Clear ultracentrifuge tubes (Beckman Coulter). Next ...
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bioRxiv - Physiology 2023Quote: ... and HDL (1.063
mL) using NaBr buffers in the Optima MAX-TL Ultracentrifuge with the TLA-100 rotor (Beckman Coulter). Complete blood count analysis was carried out using Heska Element HT5. -
bioRxiv - Molecular Biology 2023Quote: ... 100 μg/mL cycloheximide) and 900 μL were added to 13 x 56 mm polycarbonate thick wall tube (Beckman Coulter). Then ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acid purification was performed using SPRI Select (BECKMAN COULTER), nucleic acid concentration was determined by Qubit High Sensitivity DNA assay (Thermo Fisher) ...
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bioRxiv - Biophysics 2022Quote: ... From each sample 100 µl lysate were transferred to a special tube for ultra-centrifugation (Beckman Coulter, 1.5 ml tube, 257448). The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80 ...
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bioRxiv - Cell Biology 2019Quote: ... The culture was harvested in to 2 × 250 ml centrifuge bottles (Nalgene, 3120-0250) and centrifuged in an Optimia XPN-100 ultra centrifuge (Beckman Coulter) with a SW 32 Ti rotor at 5,000 × g for 20 min at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... CD14 BV605 (1:100) and CD11b PC5 (1:100, Beckman Coulter). Cells were analyzed on the FACSCelesta (BD).
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bioRxiv - Microbiology 2019Quote: ... Cytoplasmic lysate was layered onto 10-50% sucrose gradients (in polysome buffer with 100 μg/mL cycloheximide) in thinwall polypropylene ultracentrifuge tubes (Beckman Coulter; 331372). Gradients were then spun at 38,000 RPM for 2 h in a SW41Ti rotor (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... After purification of nucleic acids by Agencourt RNA Clean XP (Beckman Coulter) and subsequent treatment with DNase I ...
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bioRxiv - Cancer Biology 2023Quote: ... 7AAD (1:100; Beckman Coulter) was used for dead cell exclusion ...
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bioRxiv - Cancer Biology 2023Quote: ... 7AAD (1:100; Beckman Coulter) was used ...
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bioRxiv - Cancer Biology 2020Quote: ... GlyA PE (1:100, Beckman Coulter), CD71 BV650 (1:100) ...
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bioRxiv - Cancer Biology 2020Quote: ... GlyA PE (1:100, Beckman Coulter), CD71 BV650 (1:100) ...
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bioRxiv - Cancer Biology 2020Quote: ... CD4 PC5 (1:100, Beckman Coulter) and CD56 BV605 (1:100) ...
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bioRxiv - Cancer Biology 2020Quote: ... GlyA PE (1:100, Beckman Coulter), CD71 BV650 (1:100) ...
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bioRxiv - Biophysics 2022Quote: ... Optima XE-100 ultracentrifuge (Beckman Coulter). Cells from 90% Percoll were called the “dehydrated cell fraction” (DF ...
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bioRxiv - Biochemistry 2022Quote: ... 000 rpm (TLA-100; Beckman Coulter) for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... by ultracentrifugation (Beckman Optima XPN-100) with a type 70Ti rotor ...