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Citations for Beckman :
1 - 50 of 1231 citations for 7 Hydroxy 4 methylcoumarin 3 acetic acid succinimidyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Cell Biology 2023Quote: ... either the dye was solubilized with 10% (v/v) acetic acid and the absorbance was measured at 570 nm in a spectrophotometer microplate reader (Beckman Coulter, Model AD340), or alternatively ...
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bioRxiv - Microbiology 2024Quote: Extracts were then diluted 20-fold (in 33% acetic acid) and their absorbance at A595 determined using a spectrophotometer (Beckman Coulter, DU-800).
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bioRxiv - Immunology 2021Quote: ... CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1 ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 °C for 3 h (SW40 Ti rotor, Beckman-Coulter, Germany), the supernatant was discarded ...
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bioRxiv - Immunology 2023Quote: ... AlexaFluor 700 anti-human CD38 (clone LS198-4-3, Beckman Coulter), APC-Cy7 anti-human CD19 (clone SJ25C1 ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C for 3 h using a SW41 rotor (Beckman Coulter). Sucrose gradients were fractionated using a Piston Gradient Fractionator (Biocomp) ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C for 3 h using a SW41 rotor (Beckman Coulter). Sucrose gradients were fractionated using a Piston Gradient Fractionator (Biocomp) ...
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bioRxiv - Bioengineering 2021Quote: ... or 7-Amino-Actinomycin D (7-AAD, Beckman Coulter, B88526) was added to the cell suspensions ...
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bioRxiv - Genomics 2020Quote: ... and separated by ultracentrifugation at 4°C for 3 hours (35300 rpm, Beckman, SW41 rotor) through a 5-50% sucrose gradient (40 mM Tris-HCl pH 8.4 ...
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bioRxiv - Biophysics 2023Quote: ... precipitated by ultracentrifugation (35,000 rpm for 3 h at 4 ° C, Beckman-Coulter L90K, USA), and then redissolved in TNE/5 buffer.
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bioRxiv - Biophysics 2020Quote: ... After centrifugation at 30,000 rpm (4 ° C for 3 h) in the SW45Ti rotor (Beckman Instruments), eight 1.5-ml fractions were collected from the top of a gradient ...
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bioRxiv - Biochemistry 2020Quote: ... Gradients were resolved at 36,000 rpm for 3 hours at 4 °C in SW41 rotor (Beckman). Both separation and fractionation of gradients used a Biocomp Gradient Station (BioComp Instruments ...
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bioRxiv - Biochemistry 2020Quote: ... subjected to centrifugation (36,000 rpm for 3 hours at 4 °C, SW-41Ti Beckman Coulter rotor) and analysed using Biocomp Gradient Station (BioComp Instruments ...
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bioRxiv - Cell Biology 2019Quote: ... and centrifuged at 200,000 g for 3 h at 4°C in a TLS55 rotor (Beckman Coulter). The sucrose gradient was fractionated manually from the top ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were centrifuged at 35,000 rpm for 3 hours at 4°C (SW41 Ti rotor; Beckman) and subsequently fractionated using a Piston Gradient Fractionator (Biocomp instruments ...
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bioRxiv - Biophysics 2023Quote: ... iVCL supernatant was centrifugated for 3 h at 25000 rpm and 4 °C (Beckman-Coulter L90K, USA). Precipitate was resuspended in the TNE buffer (0.1 M Tris-HCl ...
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Rhes protein transits from neuron to neuron and facilitates mutant huntingtin spreading in the brainbioRxiv - Neuroscience 2021Quote: ... The gradient was spun for 3 h at 180,000 × g (average) at 4°C (SW41 Beckman ultra-centrifuge). After the spin the top of the gradient was removed and discarded ...
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bioRxiv - Microbiology 2021Quote: ... The outer membrane vesicles (OMVs) were then isolated by ultracentrifugation (140 000xg, 4°C) for 3 hours (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were ultracentrifuged at 28.000g for 3 h at 4°C in a SW 28 Ti rotor (Beckman). 1.13 mL fractions were then collected using the Gradient Station model 153 ...
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bioRxiv - Biochemistry 2023Quote: ... Gradients were centrifuged at 35K rpm for 3 hrs at 4°C in a SW-41Ti rotor (Beckman) with maximum acceleration and no brake using a Beckman Optima L-90 Ultracentrifuge ...
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bioRxiv - Biochemistry 2023Quote: ... lysosomes and mitochondria were enriched by centrifugation of the pooled PNS at 56,500 ×g for 7 min at 4 °C (Beckman-Coulter, 70 Ti fixed-angle rotor). The supernatant was removed ...
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bioRxiv - Biophysics 2021Quote: ... The VP2 capsid protein fractions were pooled and subjected to centrifugation at 28,000 rpm (139,065 × g) for 3 h at 4°C (Sw40Ti rotor, Beckman Coulter). The pellet was resuspended in 50 mM Tris (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter, # 331362). The polysome fractions were collected using the Foxy R1 Fraction Collector and immediately snap frozen with liquid nitrogen and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μg/ml cycloheximide) and centrifuged for 3 h at 55,000 g and 4 °C in a Type 70 Ti rotor (Beckman). The pellet was dissolved in 1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were spun for 3 hours at 4° C and 36,000 RPM (221,632 x g) on SW41-Ti rotor (Beckman Coulter). Monosome fractions were isolated from each sucrose gradient with Brandel Density Gradient Fractionator using 1.5 mL/min flow speed and 60% CsCl ...
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bioRxiv - Microbiology 2019Quote: ... Gradients were centrifuged at 210,000 x g during 3 h at 4°C using a SW-41 swinging bucket rotor (Beckman) in an Optima L-100 XP Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was solubilized for 3 hours at 4°C before separation by ultracentrifugation at 100,000 g (Ti45, Beckman) for 45 min ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was centrifuged for 3 h at 115,000 g and 4°C using the XPN-80 ultracentrifuge equipped with a Ti70 rotor (Beckman). EV pellets were resolved in 1 mL ice-cold PBS (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The tube was centrifuged for 3 hours at 150’000 x g (4°C) using a SW 32.1 Ti swinging bucket (369651, Beckman Coulter). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... the liposomes were isolated by centrifugation for 3 h 40 mins at 55,000 rpm at 4°C in a SW 60 rotor (SW 60 Ti, Beckman). 20 µl were harvested from the top of the gradient and mixed with 8 µl of 4x Laemmli buffer (final 62.5 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The post-nuclear supernatants were ultracentrifuged using SW41Ti rotor at 100,000 g for 3 h at 4 °C on Optima L-90K (Beckman Coulter). The fraction between 0.4–0.5 M sucrose was collected ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... UV-inactive SARS-CoV-2 was then partially-purified using a 20% sucrose cushion centrifuged at 175,000 x g for 3-4 hr using SW32Ti rotor (Beckman Coulter). The sample that has passed through the sucrose was collected and concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were centrifuged at 4°C for 3 h at 100,000 g by using Avanti J-30XP (Beckman, USA), and the fractions were collected using a Brandel Fractionation System (USA ...
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bioRxiv - Microbiology 2022Quote: ... 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter, USA) through a 20% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... using 10-40 % sucrose gradients in buffer A after 3 hours of centrifugation at 35,000 rpm and 4 °C in SW41 rotor (Beckman Coulter). To isolate ribosomes for structural analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were ultra-centrifuged for 3 h at 175,000g at 4°C in a SW41Ti rotor (Beckman-Coulter, USA) and analyzed by measuring the absorbance continuously at 260 nm using a piston gradient fractionator (Biocomp Instruments) ...
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bioRxiv - Molecular Biology 2022Quote: ... and spun at 40,000 rpm for 3 hours at 4°C using a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter). Sucrose gradient fractionation was performed by using a Brandel Density Gradient Fractionation System ...
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bioRxiv - Molecular Biology 2023Quote: 8 mL of viral supernatant on a 3 mL of 20% sucrose cushion were ultracentrifugated for 3 h at 100,000 x g at 4°C using an Optima L80-XP instrument (Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Synthetic Biology 2023Quote: ... The gradients were ultra-centrifuged at 41,000 rpm for 3 hours at 4°C (Optima L-80 XP ultracentrifuge (Beckman-Coulter)) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
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bioRxiv - Microbiology 2023Quote: ... Each tube was centrifuged for 3 hours at 150’000 x g (4°C) using an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW 32 Ti swinging bucket (369650 ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained supernatant was layered onto 30 w/v% sucrose solution in ultracentrifugation tubes and subjected to ultracentrifugation at 100,000 × g for 3 h at 4°C using SW32Ti rotor (Beckman courter). The collected sucrose solutions were mixed with PBS and washed by ultracentrifugation at 100,000 × g for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... The sucrose density gradient was centrifuged at 50,000 rpm for 3 hours at 4°C in a TLS-55 swinging bucket rotor (Beckman Coulter) with minimum acceleration up to 5,000 RPM and deceleration with no break ...
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bioRxiv - Bioengineering 2023Quote: ... For performing cryogenic transmission electron microscopic imaging EVs were centrifuged and washed in PBS at 100,000 x g for 3 hours at 4°C in a SW41 Ti rotor (Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... The concentrated CFS was then ultracentrifuged at 100,000 ˗ g and 4 °C for 3 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) ...
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bioRxiv - Microbiology 2021Quote: ... incubated samples for 7 min, centrifuged (650 x g, 7 min, RT) and measured them by flow cytometry (Cytomics FC500, Beckman Coulter, USA) after resuspension in Tyrode’s buffer ...
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bioRxiv - Cell Biology 2019Quote: Viability of unlabeled and ferumoxytol-labeled murine MSCs (n=4) and human MSCs (n=3) was assessed by Trypan blue dye exclusion using ViCell (Beckman Coulter).
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bioRxiv - Molecular Biology 2019Quote: ... followed by 3 h ultracentrifugation at 100,000 g and 4 °C using SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Resulting pellets were resuspended in 100 µl PBS and stored at −80 °C until further use ...