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Citations for Beckman :
1 - 39 of 39 citations for 7 Bromotryptamine HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... or 7-Amino-Actinomycin D (7-AAD, Beckman Coulter, B88526) was added to the cell suspensions ...
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bioRxiv - Cell Biology 2024Quote: ... 10 mM Tris-HCl) in 13.2 ml centrifuge tubes (Beckman Coulter Inc., 344059) and centrifuged for 2.5 h at 30000 RPM (∼154 000 g ...
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bioRxiv - Microbiology 2021Quote: ... incubated samples for 7 min, centrifuged (650 x g, 7 min, RT) and measured them by flow cytometry (Cytomics FC500, Beckman Coulter, USA) after resuspension in Tyrode’s buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 7-color cell sorter using Summit software (Beckman Coulter Life Sciences). The sorted RFP+ only and RFP+/GFP+ cell populations were collected and replated on coverslips with pre-seeded HA cells in NDM ...
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bioRxiv - Microbiology 2020Quote: ... 10 mM Tris HCl buffer (pH 7.4) (TNE buffer) in Ultra-Clear 38 ml tubes (Beckman Coulter). Samples were centrifuged at 25,000 rpm for 2h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Apoptosis was analyzed using Annexin V-FITC / 7-AAD kit (Beckman Coulter) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mM Tris-HCl in DEPC-treated water) in a thick-walled ultracentrifuge tube (Beckman Coulter, Cat # 355631) and spun at 107,000 RCF ...
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bioRxiv - Microbiology 2022Quote: ... phage suspension was layered on top of a 5–20 % sucrose (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) and centrifuged (Beckman Optima L-K90 ...
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bioRxiv - Microbiology 2022Quote: ... The light-scattering zone was collected and further purified in 20– 70 % sucrose gradient (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) by centrifugation (Beckman Optima L-K90 ...
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bioRxiv - Immunology 2020Quote: ... 10 mM Tris-HCl, pH 8.1), chromatin was eluted (1% SDS, 0.1M NaHCO3)and recovered with Agencourt AMPure XP beads (Beckman Coulter). Bound chromatin and input DNA were treated with RNase H (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... The mixtures were loaded into ultracentrifuge tubes (7 x 20 mm, Beckman Coulter) and fractionated at 100,000Xg using a Beckman TL-100 ultracentrifuge ...
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bioRxiv - Cancer Biology 2022Quote: ... GFP+7-AAD- cells were sorted using MoFlo XDP Cell Sorter (Beckman Coulter) or FACSAria III Cell Sorter (Becton Dickinson) ...
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bioRxiv - Cancer Biology 2022Quote: ... made of Sucrose Gradient Buffer (25mM Tris-HCl, pH 7.5, 150mM NaCl, 12mM MgCl2, 1mM DTT) in Ultra-Clear ultracentrifugation tubes (Beckman Coulter). The cytosolic fraction samples were fractionated by ultracentrifugation for 2h 45min at 39,000rpm and at 4°C in a Beckman Optima ultracentrifuge with a SW41 Ti swinging bucket rotor at the following settings ...
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bioRxiv - Cell Biology 2020Quote: 5-20% sucrose gradients were prepared on a Biocomp Gradient Station IP from 5% and 20% solutions of sucrose in gradient buffer (50mM Tris HCl pH7.5, 150mM NaCl, 1mM EDTA) to Seton tubes (Beckman Coulter). Single cell suspensions of approximately 3×108 cells from thymus and bone marrow were obtained as above ...
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bioRxiv - Neuroscience 2022Quote: ... 10 mM Tris-HCl) and centrifugated at 24,000 rpm for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS ...
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Dopaminoceptive D1 and D2 neurons in ventral hippocampus arbitrate approach and avoidance in anxietybioRxiv - Neuroscience 2023Quote: ... 10 mM Tris-HCl) and centrifugated at 107,000 g for 1 hour at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS supplemented with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT and 10 mM Tris-HCl in DEPC-treated water was added to the bottom of the lysis solution in ultracentrifuge tubes (Beckman Coulter) to form a gradient ...
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bioRxiv - Biochemistry 2024Quote: ... 50 mM Tris-HCl pH 7.4) and ultracentrifuged at 250 000 x g for 2 h (Beckman Optima TL 100 Ultracentrifuge). After ultracentrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... PEG was removed via centrifugation at 136,000 xg for 7 minutes in TLA100.3 (Beckman Ultracentrifuge), and supernatant was pre-cleared by incubating with Protein A Sepharose beads (GE LifeSciences #17127901 ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were transferred to 7 x 20mm polycarbonate thick-wall tubes (Beckman Coulter, Krefeld, Germany) for ultra-centrifugation (centrifuge Optima TLX with rotor TLA 100 ...
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bioRxiv - Cancer Biology 2023Quote: ... After 5-7 days cells were counted using a Z1 Coulter Particle Counter (Beckman Coulter).
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bioRxiv - Molecular Biology 2020Quote: ... diluted in 9 mL of 10 mM Tris-HCl buffer and centrifuged for 45 min at 45.000 rpm (Beckman 70.1 Ti Rotor). Pellets were suspended in the same buffer and analyzed by SDS-PAGE using 12.5% polyacrylamide gel ...
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bioRxiv - Immunology 2023Quote: ... lysates were fractionated on a linear sucrose gradient (7–47%) using the SW-41Ti rotor (Beckman Coulter) at 221,633x g for 2lllhours at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Sucrose gradients (7-47%) were centrifuged at 39,000 rpm with a SW41-Ti Rotor (Beckman Coulter, Fullerton, CA) for 90 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound protein was separated from liposomes by transferring 100μl of this mixture to 7×20mm PC ultracentrifuge tubes (Beckman) and carefully layering an equal volume of 0.75M sucrose buffer followed by 25μl of sucrose-free HK buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were mounted on bottomless 35 mm dishes with a 7 mm microwell using silicone vacuum grease (Beckman Coulter). Conditioned media from the coverslip’s well ...
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bioRxiv - Neuroscience 2023Quote: ... Fractions were washed by diluting with 7 mL of PBS and ultracentrifuged at 100,000 x g (Type 70.1 Ti rotor, Beckman Coulter) for 2 h ...
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bioRxiv - Cancer Biology 2022Quote: ... was removed using right-side size selection with AMPure XP beads at a 1:1 and 7:1 bead:sample ratio (Beckman Coulter) described previously26 ...
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bioRxiv - Genomics 2020Quote: ... PCR was performed (7 cycles) with standard Illumina paired-end primers and the amplicons were then purified with AMPure XP beads (Beckman). The length ...
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bioRxiv - Genetics 2023Quote: ... Libraries were amplified using 5-7 cycles of PCR using KAPA HiFi DNA polymerase and purified with 1.1X SPRI (Beckman Coulter). Nucleosome profiles were manually inspected using a Tapestation with the D1000 Screen Tape system (Agilent) ...
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bioRxiv - Immunology 2023Quote: Flow cytometry analysis was performed at the end of the 7 day T cell progenitor differentiation stage using a CytoFLEX LX cytometer (Beckman), and data were compensated using single-stained controls in CytExpert v2.3 ...
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bioRxiv - Systems Biology 2019Quote: ... then resuspended in 1% BSA-PBS containing 100ng/mL propidium iodide or 200ng/mL 7-AAD and proceeded with sorting using a MoFlo Astrios (Beckman Coulter) or FACSJazz (BD Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... with anti-Met-PE (clone eBioclone 7, eBiosciences) or isotype control (clone eBRG1, eBiosciences) or permeabilized (IntraPrep Permeabilization kit, Beckman Coulter) before staining (total Met) ...
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bioRxiv - Genomics 2023Quote: ... Five million cells were harvested at 315xg for 7 minutes in a 15 mL centrifuge tube (Beckman Coulter Allegra X-14R) and stored at -80°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was then applied on a continuous 7-47% sucrose gradient (mol weight/volume) in ultra clear tubes (Beckman & Coulter, #331372) and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample was centrifuged at a speed of 180000Xg (180000 RCF) in 7 mm x 20 mm polycarbonate centrifugation tubes (Beckman Coulter, USA) for at least 2h ...
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bioRxiv - Biochemistry 2019Quote: ... in iso-osmolar RB150/Mg2+ (containing a reduced level (2%) of glycerol) and 80µl were transferred to 7 × 20 mm polycarbonate tubes (Beckman Coulter, Brea CA), overlaid with 80µl of 35% ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 M sucrose in HK was added to the reaction to a final concentration of 1 M (“Input” sample) and transferred to 7 × 20 mm polycarbonate centrifuge tubes (Beckman Coulter cat #343775). HK buffer with 0.75 M sucrose was gently laid on top of the reaction mixture followed by a layer of HK ...
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bioRxiv - Biochemistry 2023Quote: ... lysosomes and mitochondria were enriched by centrifugation of the pooled PNS at 56,500 ×g for 7 min at 4 °C (Beckman-Coulter, 70 Ti fixed-angle rotor). The supernatant was removed ...