Labshake search
Citations for Beckman :
1 - 50 of 1147 citations for 7 1H Pteridinone 4 amino 6 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... or 7-Amino-Actinomycin D (7-AAD, Beckman Coulter, B88526) was added to the cell suspensions ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by centrifugation at ∼28500g for 1h at 4°C (Beckman Coulter centrifuge, JA-14) in order to remove cell debris ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by lysate centrifugation at ∼28500g for 1h at 4°C (Beckman Coulter centrifuge, JA-14). The supernatant was incubated for 45min ...
-
bioRxiv - Biochemistry 2023Quote: ... the lysate was centrifuged at ∼18000g for 1h at 4°C (Beckman Coulter centrifuge, JA-20) and the supernatant incubated for 45min ...
-
bioRxiv - Neuroscience 2022Quote: ... Insoluble material was removed by ultracentrifugation (1h, 4°C, 40,000 rpm, Ti70 rotor, Beckman Coulter Cat#337922). The fragments were immobilized onto glutathione Sepharose (Millipore/Cytiva Cat#17-5132-02 ...
-
bioRxiv - Biochemistry 2023Quote: ... After ultracentrifugation for 1h at 258,488 x g at 4°C using a TLS-55 rotor (Beckman), the fractions were analysed by SDS-PAGE and the positive fractions were concentrated ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was then centrifuged at ∼28500g for 1h at 4°C (Beckman Coulter centrifuge, JA-14) and the supernatant incubated for 1h ...
-
bioRxiv - Microbiology 2020Quote: ... the 100,000xg centrifugation was performed for 1h at 4°C using a SW41 TI swinging bucket rotor (Beckman Coulter). The pellet was then resuspended in 50 μL or 1500 μL of D-MEM for subsequent analysis.
-
bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter ...
-
bioRxiv - Cancer Biology 2021Quote: ... The pellet was washed with PBS and ultracentrifuged at 100,000 × g 1h at 4°C to obtain small vesicles (TL-100 Ultracentrifuge, Beckman Coulter). The final pellet was resuspended in PBS for subsequent experiments ...
-
bioRxiv - Microbiology 2021Quote: ... The membrane fraction was prepared using ultracentrifugation (45,000 rpm, 4 °C, 1h; Optima-90 ultracentrifuge, Beckman-Coulter, Brea, CA, USA) and washed once in 200 mM ABC buffer ...
-
bioRxiv - Microbiology 2021Quote: ... The resting supernatants were filtered through 0.45µm syringe filters and ultracentrifuged at 100,000 x g for 1h at 4°C (SW41 Ti swinging-bucket rotor, Beckman Coulter). The supernatant was discarded and the pellet suspended in 0.22 µm-pore filtered or 0.02 µm-pore filtered (for Flow Cytometry analysis ...
-
bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter, 369650; Optima XE, Beckman Coulter). Phagosome-containing beads float at the interface between the 10% and 25% layers ...
-
bioRxiv - Neuroscience 2023Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
-
bioRxiv - Microbiology 2022Quote: ... and the viral particles were concentrated by ultracentrifugation for 1h at 30,000 rpm at 4°C in an SW40 rotor (Beckman, Fullerton, CA). Viral pellets were resuspended in TNC buffer (10 mM Tris-HCl [pH 7.5] ...
-
bioRxiv - Neuroscience 2021Quote: ... KG, Staufen, Germany) (position 6, 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge ...
-
bioRxiv - Plant Biology 2023Quote: ... The disrupted cells were subjected to ultracentrifugation at 4°C and 235000 g for 1h (Beckman Optima™ L-80 XP, rotor Ti45). The resulting supernatant was loaded onto an Äkta purifier system (GE HealthCare ...
-
bioRxiv - Microbiology 2021Quote: ... lysates were placed on top of a 20-50% sucrose gradient and sedimented at 35,000 rpm (218,000x g) for 6 hours at 4°C on SW40Ti rotor (Beckman). For flotation ...
-
bioRxiv - Molecular Biology 2019Quote: ... and centrifuged for 6 hours at 55,000 rpm at 4°C on a TLS-55 rotor (Beckman Coulter) in a TL-100 ultracentrifuge (Beckman Coulter) ...
-
bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
-
bioRxiv - Immunology 2021Quote: Cells were analyzed using 4-to 6-color direct immunofluorescence staining on a Cytoflex LX or S (Beckman Coulter) flow cytometer ...
-
bioRxiv - Cancer Biology 2021Quote: ... Libraries were further amplified 4–6 PCR cycles as described [43] and purified using AMPure XP beads (Beckman Coulter). ATAC-seq libraries were subsequently sequenced 2 x 75 bp on an Illumina NextSeq500.
-
bioRxiv - Microbiology 2022Quote: ... The gradient was spun at 45,000 rpm (~200,000 x g) for 6 hours at 4 °C in a SW60Ti rotor (Beckman Coulter). Successive 0.4 mL fractions were collected by pipetting and their densities measured ...
-
bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were seeded into 6-well plates at a concentration of 4×105 cells per well and counted cumulatively with a Coulter counter (Beckman) every three days for two weeks ...
-
bioRxiv - Cell Biology 2020Quote: ... Both subfractions were centrifuged in a Ti50 rotor at 225,000g for 1h (Beckman Type 70.1Ti rotor) at 4C ...
-
bioRxiv - Biochemistry 2022Quote: ... Insoluble material was removed by ultracentrifugation at 158,000 ×g for 1h (Beckman L-90K, Ti70 rotor), and membranes were filtered with a 0.45 μm syringe filter and applied to a 0.5 mL anti-FLAG M2 affinity gel in a column (Millipore Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... lysosomes and mitochondria were enriched by centrifugation of the pooled PNS at 56,500 ×g for 7 min at 4 °C (Beckman-Coulter, 70 Ti fixed-angle rotor). The supernatant was removed ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were grown for 4-6 days and counted using an Incucyte (Essen Biosciences) or a Z2 Coulter Counter (Beckman Coulter). Small molecule dose-response curves were printed using an D300e Digital Dispenser (HP Specialty Printing Systems ...
-
bioRxiv - Molecular Biology 2019Quote: ... was loaded onto 4 ml of a 10% −30% (w/v) linear sucrose gradient and centrifuged at 74,000g at 4°C for 6 h (Beckman MLS-50 rotor). Fractions (250 µl ...
-
bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
-
bioRxiv - Microbiology 2020Quote: ... 1mM dithiothreitol and 100μg/ml cycloheximide) and spun for 1h at 100K RPM using TLA-110 rotor (Beckman) at 4c ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by spinning at 3,000xg followed by 20,000xg for 10min each and supernatant were loaded on a 30% sucrose cushion and spun for 1h at 31,200 r.p.m in SW60 rotor (Beckman Coulter). The non-polysomal supernatant was removed and polysomes collected below the sucrose layer was diluted with the hypotonic buffer [10mM HEPES pH 7.8 ...
-
bioRxiv - Microbiology 2020Quote: ... 1mM dithiothreitol and 100μg/ml cycloheximide) and spun for 1h at 100K RPM using TLA-110 rotor (Beckman) at 4c ...
-
bioRxiv - Microbiology 2021Quote: ... incubated samples for 7 min, centrifuged (650 x g, 7 min, RT) and measured them by flow cytometry (Cytomics FC500, Beckman Coulter, USA) after resuspension in Tyrode’s buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... Fractions were diluted and washed in PBS for 1h at 100,000 x g in a TLA 110 rotor (Beckman). Fractions were resuspended in 35 ul of PBS ...
-
bioRxiv - Biophysics 2021Quote: ... 20-24 h), the samples were ultracentrifuged (1h, 288’000 x g) (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor) to remove large aggregates ...
-
bioRxiv - Developmental Biology 2023Quote: ... the supernatant was centrifuged in a TLA100.4 fixed angle rotor at 100000 g for 1h at 37°C in an Optima TL ultracentrifuge (Beckman). The supernatant containing globular actin was collected ...
-
bioRxiv - Biochemistry 2020Quote: ... the crude mitochondria were undergone a sucrose gradient in SEM buffer for 1h at 139,400 x g with an SW40 Ti rotor (Beckman Coulter). Mitochondrial band was pooled and stored at −80 °C.
-
bioRxiv - Cell Biology 2020Quote: ... The supernatants were collected and subjected to high-speed centrifugation at 100,000 × g for 1h (TLS-55 rotor, OptimaTMMAX Ultracentrifuge; Beckman Coulter, Germany). The supernatants (S100 ...
-
bioRxiv - Neuroscience 2023Quote: ... 7-color cell sorter using Summit software (Beckman Coulter Life Sciences). The sorted RFP+ only and RFP+/GFP+ cell populations were collected and replated on coverslips with pre-seeded HA cells in NDM ...
-
bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
-
bioRxiv - Neuroscience 2022Quote: ... 6 colors cytometer Gallios (Beckman Coulter®). Gating strategy was determined based on the specific staining of markers for microglial population18.
-
bioRxiv - Cell Biology 2021Quote: ... Apoptosis was analyzed using Annexin V-FITC / 7-AAD kit (Beckman Coulter) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2019Quote: ... To collect the membrane fraction the cleared supernatant was centrifuged at 100,000g at 4°C for 1h in a Beckman Optima MAX ultracentrifuge with a TLA110 rotor using polycarbonate thick wall centrifuge tubes (Beckman coulter ref. 362305). The supernatant that contains soluble proteins was removed and the pellet-containing membranes was resuspended in 1ml ice-cold homogenization buffer by sonication and re-centrifuged at 100,000g ...
-
bioRxiv - Neuroscience 2022Quote: ... gradient (6 ml 17 %, 6 ml 25 %, 5 ml 40 % and 5 ml 60 %) contained within an OptiSeal tube (Beckman,). Gradients were spun at 242 000 g at 16 °C and the viral fraction was harvested from the 40 % fraction with a 21-gauge needle ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and supernatants were ultracentrifuged at 37 000 rpm (~100 000 g) and 10°C for 1h (Beckman Optima LE-80 K; rotor 70.1.Ti). Pellets were resuspended in 100μL of buffer (10 mM Tris-HCL ...
-
bioRxiv - Biophysics 2021Quote: ... Following incubation (37 °C, 20-24 h), the samples were ultracentrifuged (1h, 288’000 x g) (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor) to remove large aggregates ...
-
bioRxiv - Cell Biology 2020Quote: ... The mixtures were loaded into ultracentrifuge tubes (7 x 20 mm, Beckman Coulter) and fractionated at 100,000Xg using a Beckman TL-100 ultracentrifuge ...