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Citations for Beckman :
1 - 50 of 985 citations for 6 Phenyl 1H imidazo 1 2 b pyrazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II ...
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bioRxiv - Biochemistry 2021Quote: ... 6 mM 2-mercaptoethanol and centrifuged with a 70Ti (Beckman coulter) rotor at 40,000 rpm for 18 hours at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II, respectively); and βHCG and AFP CMIA-ARCHTECT I 4000.The assays were performed as recommended by the manufacturers ...
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bioRxiv - Biochemistry 2023Quote: ... followed by centrifugation at ∼28500g for 1h at 4°C (Beckman Coulter centrifuge, JA-14) in order to remove cell debris ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... Both subfractions were centrifuged in a Ti50 rotor at 225,000g for 1h (Beckman Type 70.1Ti rotor) at 4C ...
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bioRxiv - Biochemistry 2022Quote: ... Insoluble material was removed by ultracentrifugation at 158,000 ×g for 1h (Beckman L-90K, Ti70 rotor), and membranes were filtered with a 0.45 μm syringe filter and applied to a 0.5 mL anti-FLAG M2 affinity gel in a column (Millipore Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... followed by lysate centrifugation at ∼28500g for 1h at 4°C (Beckman Coulter centrifuge, JA-14). The supernatant was incubated for 45min ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was centrifuged at ∼18000g for 1h at 4°C (Beckman Coulter centrifuge, JA-20) and the supernatant incubated for 45min ...
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bioRxiv - Neuroscience 2022Quote: ... Insoluble material was removed by ultracentrifugation (1h, 4°C, 40,000 rpm, Ti70 rotor, Beckman Coulter Cat#337922). The fragments were immobilized onto glutathione Sepharose (Millipore/Cytiva Cat#17-5132-02 ...
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bioRxiv - Biochemistry 2023Quote: ... After ultracentrifugation for 1h at 258,488 x g at 4°C using a TLS-55 rotor (Beckman), the fractions were analysed by SDS-PAGE and the positive fractions were concentrated ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was then centrifuged at ∼28500g for 1h at 4°C (Beckman Coulter centrifuge, JA-14) and the supernatant incubated for 1h ...
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bioRxiv - Microbiology 2022Quote: Chlorophyll (a+b) was extracted from 1 ml of algal or algal-bacterial pellets with ethanol (1 ml) and measured spectrophotometrically (Beckman Coulter, DU 800) (Wintermans and De Mots ...
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bioRxiv - Microbiology 2020Quote: ... 1mM dithiothreitol and 100μg/ml cycloheximide) and spun for 1h at 100K RPM using TLA-110 rotor (Beckman) at 4c ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by spinning at 3,000xg followed by 20,000xg for 10min each and supernatant were loaded on a 30% sucrose cushion and spun for 1h at 31,200 r.p.m in SW60 rotor (Beckman Coulter). The non-polysomal supernatant was removed and polysomes collected below the sucrose layer was diluted with the hypotonic buffer [10mM HEPES pH 7.8 ...
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bioRxiv - Microbiology 2020Quote: ... 1mM dithiothreitol and 100μg/ml cycloheximide) and spun for 1h at 100K RPM using TLA-110 rotor (Beckman) at 4c ...
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bioRxiv - Cancer Biology 2019Quote: ... CD10 PE-TR (B-Ly6; Beckman Coulter; www.beckmancoulter.com); CD14 FITC (M5E2) ...
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bioRxiv - Immunology 2020Quote: ... a lyse-wash protocol (Optilyse B, Beckman-Coulter) was performed ...
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bioRxiv - Microbiology 2020Quote: ... were added into cells (1×106 cells/sample in 100 μL) and incubated for 60 min at 2-6°C and analyzed using flow cytometry (CytoFLEX, Beckman Coulter).
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bioRxiv - Neuroscience 2023Quote: ... After PCR the samples were combined into small pool of 2-6 samples and size selection was carried out by adding 0.55x volume of AMPure XP beads (Beckman Coulter, #A68831). The concentrations of purified library pools were quantified with Collibri™ Library Quantification Kit (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... the 100,000xg centrifugation was performed for 1h at 4°C using a SW41 TI swinging bucket rotor (Beckman Coulter). The pellet was then resuspended in 50 μL or 1500 μL of D-MEM for subsequent analysis.
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bioRxiv - Cell Biology 2020Quote: ... Fractions were diluted and washed in PBS for 1h at 100,000 x g in a TLA 110 rotor (Beckman). Fractions were resuspended in 35 ul of PBS ...
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bioRxiv - Biophysics 2021Quote: ... 20-24 h), the samples were ultracentrifuged (1h, 288’000 x g) (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor) to remove large aggregates ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter ...
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bioRxiv - Developmental Biology 2023Quote: ... the supernatant was centrifuged in a TLA100.4 fixed angle rotor at 100000 g for 1h at 37°C in an Optima TL ultracentrifuge (Beckman). The supernatant containing globular actin was collected ...
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bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
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bioRxiv - Immunology 2020Quote: ... CD103 (APC, B-Ly7, eBioscience, or biotin, 2G5, Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... The pellet was washed with PBS and ultracentrifuged at 100,000 × g 1h at 4°C to obtain small vesicles (TL-100 Ultracentrifuge, Beckman Coulter). The final pellet was resuspended in PBS for subsequent experiments ...
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bioRxiv - Biochemistry 2020Quote: ... the crude mitochondria were undergone a sucrose gradient in SEM buffer for 1h at 139,400 x g with an SW40 Ti rotor (Beckman Coulter). Mitochondrial band was pooled and stored at −80 °C.
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bioRxiv - Microbiology 2021Quote: ... The membrane fraction was prepared using ultracentrifugation (45,000 rpm, 4 °C, 1h; Optima-90 ultracentrifuge, Beckman-Coulter, Brea, CA, USA) and washed once in 200 mM ABC buffer ...
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bioRxiv - Microbiology 2021Quote: ... The resting supernatants were filtered through 0.45µm syringe filters and ultracentrifuged at 100,000 x g for 1h at 4°C (SW41 Ti swinging-bucket rotor, Beckman Coulter). The supernatant was discarded and the pellet suspended in 0.22 µm-pore filtered or 0.02 µm-pore filtered (for Flow Cytometry analysis ...
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bioRxiv - Neuroscience 2023Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter, 369650; Optima XE, Beckman Coulter). Phagosome-containing beads float at the interface between the 10% and 25% layers ...
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bioRxiv - Immunology 2023Quote: ... and B cells were determined using Flow-Count fluorospheres (Beckman) and a Cytoflex flow cytometer (Beckman ...
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bioRxiv - Microbiology 2022Quote: ... and the viral particles were concentrated by ultracentrifugation for 1h at 30,000 rpm at 4°C in an SW40 rotor (Beckman, Fullerton, CA). Viral pellets were resuspended in TNC buffer (10 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were collected and subjected to high-speed centrifugation at 100,000 × g for 1h (TLS-55 rotor, OptimaTMMAX Ultracentrifuge; Beckman Coulter, Germany). The supernatants (S100 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Immunology 2021Quote: ... live B cells were sorted on an Astrios EQ (BeckMan Coulter) into PBS containing 30% FBS (Supplementary Data 2) ...
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bioRxiv - Immunology 2021Quote: ... Stained B cells were analyzed using a Navios cytometer (Beckman coulter). The phenotype of the cells was analyzed using Kaluza Flow Cytometry Analysis and FlowJo software.
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bioRxiv - Synthetic Biology 2023Quote: ... or a CytoFLEX-S (V-B-Y-R) instrument (Beckman Coulter), according to the manufacturers’ protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... Inhibin B was quantified through an enzyme-linked immunosorbent assay (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... 6 colors cytometer Gallios (Beckman Coulter®). Gating strategy was determined based on the specific staining of markers for microglial population18.
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bioRxiv - Microbiology 2021Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 μL of sterile water ...
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bioRxiv - Microbiology 2022Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 µL of RNAase free water ...
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bioRxiv - Biochemistry 2019Quote: ... To collect the membrane fraction the cleared supernatant was centrifuged at 100,000g at 4°C for 1h in a Beckman Optima MAX ultracentrifuge with a TLA110 rotor using polycarbonate thick wall centrifuge tubes (Beckman coulter ref. 362305). The supernatant that contains soluble proteins was removed and the pellet-containing membranes was resuspended in 1ml ice-cold homogenization buffer by sonication and re-centrifuged at 100,000g ...
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bioRxiv - Plant Biology 2023Quote: ... The disrupted cells were subjected to ultracentrifugation at 4°C and 235000 g for 1h (Beckman Optima™ L-80 XP, rotor Ti45). The resulting supernatant was loaded onto an Äkta purifier system (GE HealthCare ...
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... PCR amplicons for pools 1 and 2 were combined for each sample and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30μL of sterile water ...
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bioRxiv - Immunology 2020Quote: ... while human B cells were CD38+/- (Beckman Coulter #6699531; 1µl per test) and CD19 (BD #557791 ...