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Citations for Beckman :
1 - 50 of 1139 citations for 6 Hydroxy 2 oxo 2H 1 benzopyran 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (6 mg/ml) and floated for 2h at 55 000 rpm in a TLS-55 rotor (Beckman) at 25°C through a sucrose gradient ...
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bioRxiv - Microbiology 2020Quote: ... filtered through 45 μm-pore size filters and concentrated onto a 20% sucrose cushion by ultracentrifugation (24,000 rpm, 2h, 6°C) using a SW32 rotor (Beckman). HIV-1-based lentiviral particles were produced by co-transfecting 293T cells with packaging (psPAX2) ...
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bioRxiv - Cell Biology 2022Quote: ... and the post-nuclear supernatant was loaded onto an 8-step discontinuous sucrose density gradient (HEPES-buffered 0.2–2 M sucrose) and centrifuged at 55,000 rpm for 2h at 4 °C using an MLS50 rotor (Beckman Coulter). Extracellular acidification rate (ECAR ...
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (5 mg/ml) and floated for 2h at 55,000 rpm in a TLS-55 rotor (Beckman) at 25 °C through a sucrose gradient ...
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bioRxiv - Biochemistry 2021Quote: ... 6 mM 2-mercaptoethanol and centrifuged with a 70Ti (Beckman coulter) rotor at 40,000 rpm for 18 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Gradients were then centrifuged for 2h 45 min on 35.000 rpm (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... the VLPs were concentrated by ultracentrifugation (40,000 rpm, 2h, 10°C, Beckman SW41 rotor), resuspended in buffer A and observed by TEM ...
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bioRxiv - Neuroscience 2024Quote: ... and centrifuged at 28,000rpm for 2h at 4 °C in a SW 41 rotor (Beckman). The virus pellet was resuspended in 60μL of PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were ultracentrifuged at 95,000 rpm at 4°C for 2h using a TLA100.2 rotor (Beckman). The pellets were resuspended in 200 µL of W-Buffer and treated with 1% SDS (Sigma cat ...
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Plasma derived cell-free mitochondrial DNA originates mainly from circulating cell-free mitochondriabioRxiv - Genomics 2021Quote: ... and finally centrifuged at 200,000 g for 2h at 4°C (Beckman MLA-130 Ultracentrifuge Rotor). After each centrifugation step ...
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bioRxiv - Neuroscience 2021Quote: ... after which the supernatant was filtered (0.45 µm filter) and centrifuged 2h at 19,400 rpm (SW28 Beckman rotor). The pellets were re-suspended in phosphate buffered saline (PBS ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Cancer Biology 2021Quote: ... at the bottom as the cushion and centrifuged at 50,000 × g for 2h using an SW32Ti rotor (Beckman Coulter). After centrifugation ...
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bioRxiv - Neuroscience 2019Quote: ... of the lysates were loaded on top of the gradients and spun at 36,000 rpm at 4°C for 2h with a SW41-Ti rotor (Beckman). Fractions from each sample were collected every 9 sec using a density gradient fractionation system (Teledyne Isco ...
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bioRxiv - Microbiology 2022Quote: ... at 4°C for 1.5-2h followed by 180,000 x g (Ti 70i rotor; Beckman Instruments, Inc., Fullerton, CA) at 4°C for 1.5-2h and resuspended in 1mL of phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... which was spun in a ultracentrifuge at 30,000 x g for 2h at 4°C (SW 60 Ti rotor, Beckman) The pellet was resuspended in 20 μl of PBS for 1 h at 4 °C ...
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bioRxiv - Pathology 2023Quote: ... and culture supernatant was concentrated by ultracentrifugation (2h at 26,000rpm; Ultraclear Tubes; SW28 rotor and Optima L-100 XP Ultracentrifuge; Beckman). Viruses were suspended in cold sterile PBS and titrated by transduction of Jurkat cells for 48h ...
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bioRxiv - Neuroscience 2024Quote: ... The total 200mL of supernatant was centrifuged at 28,000rpm for 2h at 40 °C in a 45Ti rotor (Beckman), which can sustain faster speeds ...
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bioRxiv - Microbiology 2020Quote: VLPs were collected after removal of cells from the enrichment culture (7,000 rpm, 20 min, Sorvall 1500 rotor) and concentrated by ultracentrifugation (40,000 rpm, 2h, 10°C, Beckman SW41 rotor). The particles were resuspended in buffer A ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by ultracentrifugation at 100,000 g for 2h in polyallomer tubes at 4 °C in a SW40 rotor (Beckman Coulter). Pelleted material was washed two times in filtered PBS at 100,000 g for 2h and re-suspended in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Filtered supernatants were concentrated by ultracentrifugation at 22.000 rpm for 2h at 4°C with OptimaTM L-90K ultracentrifuge (Beckman Coulter) and stored at −80°C (never refrozen).
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bioRxiv - Biochemistry 2020Quote: ... One was through twice daily measurement of 2 or 3 levels of QC specimens obtained from Beckman Coulter Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated for 2h at 20°C in an AN60-Ti rotor in the Optima-AUC analytical ultracentrifuge (Beckman Coulter) before data acquisition for 15h at 42,000 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradients were centrifuged at 40000 rpm for 2h at 4°C in a SW 60 Ti swinging-bucket rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Microbiology 2024Quote: ... 450 µl of the lysate was carefully layered on top of the sucrose gradient and centrifuged at 35000 rpm (210000g) for 2h on a pre-chillled SW40 Ti rotor with the OptimaXL-100K ultracentrifuge system (Beckman Coulter). After the centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... were added into cells (1×106 cells/sample in 100 μL) and incubated for 60 min at 2-6°C and analyzed using flow cytometry (CytoFLEX, Beckman Coulter).
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bioRxiv - Neuroscience 2023Quote: ... After PCR the samples were combined into small pool of 2-6 samples and size selection was carried out by adding 0.55x volume of AMPure XP beads (Beckman Coulter, #A68831). The concentrations of purified library pools were quantified with Collibri™ Library Quantification Kit (ThermoFisher ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were purified by using 1.3X Agencourt AMPure XP beads (2 or 3 cycles; Beckman Coulter) and evaluated by using an Agilent High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Microbiology 2019Quote: ... and adding this to 3 ml sterile inoculum water (Beckman Coulter, Inc. Brea, CA, cat no B1015-2) and 840 μl phenol red solution ...
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bioRxiv - Biochemistry 2021Quote: ... Lentivirus from the cell supernatant was collected 24h later by ultracentrifugation with a Sw28 rotor (Beckman Coulter; 22,000 rpm, 2h, 4°C). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... The plates were then centrifuged at 2000 rpm (931 x g) for 2h at 30°C (Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A) ...
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bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
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bioRxiv - Microbiology 2020Quote: ... by centrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2h at 4°C using a Beckman SW28 rotor (Beckman Coulter, Brea, CA, USA). Supernatants were aspirated and the pellets were re-suspended in PBS (pH 7.4) ...
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bioRxiv - Neuroscience 2022Quote: ... The material in the flow-through of the washing steps was pelleted by ultracentrifugation (at 100,000g for 2h; Optima MAX-XP Ultracentrifuge from Beckman Coulter equipped with a TLA55 rotor) before lysis as described (Théry et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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bioRxiv - Genomics 2021Quote: ... samples were purified with 45 µl of Agencourt AMPure XP magnetic beads with a sample to beads ratio of 3:2 (Beckman Coulter), normalized by Qubit quantification and pooled to generate a 4 nM library ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... Bacteria cultures were collected and discarded by centrifugation for 60 min at 4,000-8,000 x g and the supernatants were subjected to high-speed centrifugation at 11,9000 x g for 2-3 h at 4°C (Beckman Coulter Optima Ultracentrifuge). The pellets containing the OMVs were washed with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... 6 colors cytometer Gallios (Beckman Coulter®). Gating strategy was determined based on the specific staining of markers for microglial population18.
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bioRxiv - Microbiology 2021Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 μL of sterile water ...
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bioRxiv - Microbiology 2022Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 µL of RNAase free water ...
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bioRxiv - Neuroscience 2019Quote: ... and 3% Percoll (1 ml each) in Polycarbonate centrifuge tubes (13 x 51 mm; Beckman Coulter). The gradients were then centrifuged for 15 min at 18,700 rcf ...
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... PCR amplicons for pools 1 and 2 were combined for each sample and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30μL of sterile water ...
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bioRxiv - Neuroscience 2022Quote: ... gradient (6 ml 17 %, 6 ml 25 %, 5 ml 40 % and 5 ml 60 %) contained within an OptiSeal tube (Beckman,). Gradients were spun at 242 000 g at 16 °C and the viral fraction was harvested from the 40 % fraction with a 21-gauge needle ...
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bioRxiv - Biophysics 2022Quote: SpeMreBs with a volume of 200 μL were polymerised for 1 to 6 h and centrifuged (100,000 rpm, 23°C, 120 min) in a TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 200 μL of water ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were centrifuged at 41399 g and 6 °C and for 1 hour (Beckman Avanti J-25 centrifuge) to obtain the cytoplasmic protein extract.
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bioRxiv - Biophysics 2023Quote: SpeMreBs in buffer S polymerized at room temperature with a volume of 200 μL were polymerized for 1–6 h and centrifuged (100,000 rpm at 23 °C for 120 min) in a TLA-100 rotor (Beckman Coulter) (2) ...
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bioRxiv - Biochemistry 2021Quote: ... The UV-Visible spectrum of each sample was recorded for both tannic acid and gallic acid for both pH’s using a DU730 UV-Visible Spectrometer (Beckman Coulter, Pasadena, CA). The spectra were recorded from 250-400 nm with 0.2 nm data intervals ...
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bioRxiv - Cancer Biology 2022Quote: ... the genomic DNA was cleaned up with AMPure XP beads at 3:1 ratio (Beckman Coulter A63880) and eluted in the 10 mM Tris-HCl (pH 8.0) ...