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Citations for Beckman :
1 - 50 of 2173 citations for 6 9 Ethano 4H imidazo 4 5 d pyridazino 1 2 a pyridazin 4 one 2 butyl 3 6 7 8 9 11 hexahydro 6 9 dimethyl 3 2' 2H tetrazol 5 yl 1 1' biphenyl 4 yl methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... KG, Staufen, Germany) (position 6, 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge ...
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bioRxiv - Cell Biology 2022Quote: ... and the post-nuclear supernatant was loaded onto an 8-step discontinuous sucrose density gradient (HEPES-buffered 0.2–2 M sucrose) and centrifuged at 55,000 rpm for 2h at 4 °C using an MLS50 rotor (Beckman Coulter). Extracellular acidification rate (ECAR ...
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bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Biochemistry 2021Quote: ... 9 s relapse) and centrifuged twice at 11,000 x g for 20 min at 4°C (Beckman) to pellet cell debris ...
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bioRxiv - Cell Biology 2021Quote: ... About 9 ml interface that came from 3×SW28 tubes (25×89 mm, Beckman Coulter) was loaded in a SW41 tube (14×89 mm ...
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bioRxiv - Biochemistry 2021Quote: ... 6 mM 2-mercaptoethanol and centrifuged with a 70Ti (Beckman coulter) rotor at 40,000 rpm for 18 hours at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... gradient (6 ml 17 %, 6 ml 25 %, 5 ml 40 % and 5 ml 60 %) contained within an OptiSeal tube (Beckman,). Gradients were spun at 242 000 g at 16 °C and the viral fraction was harvested from the 40 % fraction with a 21-gauge needle ...
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bioRxiv - Immunology 2023Quote: ... The ratio of Ca2+-bound Indo-1 versus Ca2+-free Indo-1 was analyzed using FlowJo 9 software (Beckman Coulter).
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (6 mg/ml) and floated for 2h at 55 000 rpm in a TLS-55 rotor (Beckman) at 25°C through a sucrose gradient ...
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bioRxiv - Immunology 2023Quote: ... Bacteria cultures were collected and discarded by centrifugation for 60 min at 4,000-8,000 x g and the supernatants were subjected to high-speed centrifugation at 11,9000 x g for 2-3 h at 4°C (Beckman Coulter Optima Ultracentrifuge). The pellets containing the OMVs were washed with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×10^6 cells counted by Multisizer 4e Coulter Counter (Beckman) were plated on a 60 mm plate and serum starved before analysis unless otherwise indicated ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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bioRxiv - Immunology 2021Quote: ... CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1 ...
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bioRxiv - Microbiology 2021Quote: ... lysates were placed on top of a 20-50% sucrose gradient and sedimented at 35,000 rpm (218,000x g) for 6 hours at 4°C on SW40Ti rotor (Beckman). For flotation ...
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bioRxiv - Molecular Biology 2019Quote: ... and centrifuged for 6 hours at 55,000 rpm at 4°C on a TLS-55 rotor (Beckman Coulter) in a TL-100 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Biochemistry 2020Quote: ... to a final volume of 1 ml and then further centrifuged (120000 g, 4°C, 2 h) in a TLA-120.2 rotor (Beckman Coulter, Brea, CA). Pellets were washed twice with 4°C cold PBS and centrifuged (120000 g ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Cat no UZ-PA-38.5-1) at 100,000 g at 4 °C for 2 h in a swinging-bucket rotor (Beckman Coulter; SW28 or SW32).
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was filtered with a membrane pore size of 0.2 μm (Whatman) and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Immunology 2021Quote: Cells were analyzed using 4-to 6-color direct immunofluorescence staining on a Cytoflex LX or S (Beckman Coulter) flow cytometer ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were further amplified 4–6 PCR cycles as described [43] and purified using AMPure XP beads (Beckman Coulter). ATAC-seq libraries were subsequently sequenced 2 x 75 bp on an Illumina NextSeq500.
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bioRxiv - Microbiology 2022Quote: ... The gradient was spun at 45,000 rpm (~200,000 x g) for 6 hours at 4 °C in a SW60Ti rotor (Beckman Coulter). Successive 0.4 mL fractions were collected by pipetting and their densities measured ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) and centrifuged for 2 hours and 15 minutes at 39000 rpm at 4°C (Beckman Coulter TLS55 rotor). Fractions corresponding to the large mitochondrial subunit were collected and subjected to buffer exchange (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... followed by ultracentrifugation at 4°C for 2 h (38000 rpm, Beckman, SW41 rotor) through a 20-60% sucrose gradient (40 mM Tris-HCl pH 8.4 ...
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bioRxiv - Biochemistry 2020Quote: ... One was through twice daily measurement of 2 or 3 levels of QC specimens obtained from Beckman Coulter Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 °C for 3 h (SW40 Ti rotor, Beckman-Coulter, Germany), the supernatant was discarded ...
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bioRxiv - Immunology 2023Quote: ... AlexaFluor 700 anti-human CD38 (clone LS198-4-3, Beckman Coulter), APC-Cy7 anti-human CD19 (clone SJ25C1 ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C for 3 h using a SW41 rotor (Beckman Coulter). Sucrose gradients were fractionated using a Piston Gradient Fractionator (Biocomp) ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C for 3 h using a SW41 rotor (Beckman Coulter). Sucrose gradients were fractionated using a Piston Gradient Fractionator (Biocomp) ...
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bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded into 6-well plates at a concentration of 4×105 cells per well and counted cumulatively with a Coulter counter (Beckman) every three days for two weeks ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 minutes at 4°C with TLA100 rotor (Beckman). 100 µg cell lysate was brought to a final volume of 250 µl BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Immunology 2021Quote: ... 1×106 thawed PBMCs were centrifuged (750×g for 5 minutes at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown for 4-6 days and counted using an Incucyte (Essen Biosciences) or a Z2 Coulter Counter (Beckman Coulter). Small molecule dose-response curves were printed using an D300e Digital Dispenser (HP Specialty Printing Systems ...
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bioRxiv - Biochemistry 2019Quote: ... After ultra-centrifugation (SW-60Ti rotor Beckman Coulter, 2 h, 100 000 rpm, 4°C) the gradient was manually fractioned in 200 μl fractions from the top to the bottom of the tube ...
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bioRxiv - Molecular Biology 2019Quote: ... was loaded onto 4 ml of a 10% −30% (w/v) linear sucrose gradient and centrifuged at 74,000g at 4°C for 6 h (Beckman MLS-50 rotor). Fractions (250 µl ...
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bioRxiv - Cell Biology 2021Quote: ... After centrifugation (285, 000 g for 2 h at 4 °C using a Beckman SW40Ti rotor), polysome profiles were recorded using an Äktaprime plus chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C for 2 h (SW 32 Ti swinging-bucket rotor, Optima LE-80K Beckman Coulter) using a 25% w/v sucrose cushion ...
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bioRxiv - Cell Biology 2022Quote: ... 4°C for 1 hour (JA 25.5 rotor, Beckman Coulter). The supernatant was kept and clarified by a 0.45 µM syringe filter (Pall Corporation) ...
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Biochemistry 2019Quote: ... Resuspended cells were lysed by French Press (8,000 psi, 4°C, 2 passages) and lysates were centrifuged (Beckman 60Ti rotor, 30 min, 50,000 rpm, 4°C). Pellets were resuspended in 100 ml of buffer B (PBS [140 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... were added into cells (1×106 cells/sample in 100 μL) and incubated for 60 min at 2-6°C and analyzed using flow cytometry (CytoFLEX, Beckman Coulter).
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bioRxiv - Neuroscience 2023Quote: ... After PCR the samples were combined into small pool of 2-6 samples and size selection was carried out by adding 0.55x volume of AMPure XP beads (Beckman Coulter, #A68831). The concentrations of purified library pools were quantified with Collibri™ Library Quantification Kit (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... and were centrifuged at 82,500 x g for 2 h at 4 °C (Beckman SW28 swing rotor). The band between 1.0 and 1.2 M sucrose was collected as the synaptosome fraction and diluted with 80 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... in 67 mM HEPES-KOH pH 7.2) after 2 h of ultracentrifugation (4 °C, 5000 RPM, Beckman SW41 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by ultracentrifugation at 70k RPM at 4⁰C for 2 h (Optima TLX ultracentrifuge, Beckman Coulter). Ribosome pellets were then resuspended in Trizol and extracted for RNA according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... centrifuged twice at 100,000 × g for 2 h at 4°C (Beckman Coulter Optima XE-90 Ultracentrifuge). The supernatant was replaced with DPBS between ultracentrifugation spins ...
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bioRxiv - Neuroscience 2021Quote: ... 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge; Beckman Coulter, Fullerton, CA) for 30 min ...
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bioRxiv - Immunology 2023Quote: Koff rates were determined as dissociation of reversible pMHC-Streptamers upon addition of D-Biotin in a flow cytometry-based assay (CyAn ADP Lx 9 color flow cytometer, Beckman Coulter) as described previously (44) ...
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bioRxiv - Microbiology 2019Quote: ... the extracts were added on top of layers in LCM buffer with 45% and 5% glycerol and ultracentrifuged at 77 000 x g for 1 h at +4°C (Beckman Coulter SW41Ti rotor). Viral nucleocapsids were recovered from the bottom of the ultracentrifuge tube ...