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Citations for Beckman :
901 - 950 of 1240 citations for ssc mir 323 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... A total of 25 μl of the PCR product was cleaned using 1X Agencourt AMPure XP beads (Beckman Coulter, Brea, USA) and eluted in 25 μl TE buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter). The second PCR was performed for 15 cycles for wild type-specific and for 21 cycles for tag-specific amplicons respectively using 60°C annealing and 30 s elongation ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were purified using 1.8X Agencourt AMPure XP PCR Purification beads according to the manufacturer’s instructions (Beckman Coulter, cat no A63881). The sheared DNA samples were quantified and qualified on a BioAnalyzer system using the DNA7500 assay kit (Agilent Technologies cat no ...
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bioRxiv - Molecular Biology 2020Quote: ... The library was amplified with 9 PCR cycles and cleaned with 0.9x AMPure® XP beads (A63881, Beckman Coulter, CA, USA) to enrich for DNA fragments shorter than 50nt ...
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bioRxiv - Molecular Biology 2020Quote: ... The target library derived from the oocyte RNA polymerase II transcripts was PCR-amplified (15 cycles for P14 WT, 16 cycles for P14 Tbpl2−/− mutant) and purified using AMPure beads (Beckman Coulter) to remove short PCR artifacts (< 200bp ...
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bioRxiv - Molecular Biology 2021Quote: ... Indexing PCR amplicons were pooled (4◻μl of each) and purified using AMPure XP beads (Beckman Coulter, California, United States) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: The obtained PCR products were purified and filtered by using AMPure XP magnetic beads with double size selection (Beckman Coulter, Inc.). In brief ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were verified with a 2% agarose gel electrophoresis and purified using AMPure XP bead (Beckman Coulter, Brea, CA, USA). The purified amplicon library was quantified and sequenced on the MiSeq Platform (Illumina ...
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bioRxiv - Genetics 2020Quote: ... PCR was then analyzed on an agarose gel and DNA was cleaned up using Ampure XP beads (Beckman Coulter, cat. A63881). For this the four PCR reactions were pooled resulting in 100 μL ...
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bioRxiv - Immunology 2021Quote: ... The 1st-round PCR product was cleaned up and followed by a double size selection step using SPRIselect reagent (Beckman Coulter) to separate protein library from cDNA library.
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bioRxiv - Neuroscience 2021Quote: ... 50 μL of PCR product from the pre-amplification step were purified using 50 μL of Ampure XP beads (Beckman Coulter), resuspended ...
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bioRxiv - Developmental Biology 2021Quote: ... PCRs were monitored by qPCR to avoid over-ampliciation and following every PCR reaction the samples were purified using SPRI beads (Beckman Coulter) and libraries were sequenced at 1:10 proportion of the transcriptome library on Illumina’s NovaSeq.
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bioRxiv - Genomics 2020Quote: ... We carried out the first round of PCR for 3 cycles and purified the reactions with Ampure XP beads (Beckman-Coulter). We then carried out a second round of PCR with primers targeting the P5 and P7 sequences only ...
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bioRxiv - Microbiology 2021Quote: ... with a final extension at 72 °C for 10 min PCR products were purified using Agencourt AMPure XP beads (Beckman Coulter) ratio 0.6× ...
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bioRxiv - Microbiology 2020Quote: ... We pooled the triplicate PCR products and the 24 μL of final volume were purified by adding 44 μL of AMPure XP beads (Beckman Coulter), mixing with pipette followed by a 5 minute incubation ...
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bioRxiv - Immunology 2020Quote: ... 2) first-round PCRs were set up using a Biomek FX liquid handling system with a 96- well head (Beckman Coulter); 3 ...
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bioRxiv - Genomics 2020Quote: ... These pooled single-cell libraries were used in an AMpure XP Bead-based Dual Bead Cleanup and Size Selection reaction (Agencourt AMPure XP PCR Purification modified Instructions for Use, Beckman Coulter). In both bead cleanup reactions ...
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bioRxiv - Molecular Biology 2023Quote: ... Size selection purifications following the ligation and amplification PCR steps were performed with 0.9× reaction volumes of Agencourt AMPure XP beads (Beckman Coulter A63880). Purified libraries were combined as an 8-sample equimolar pool containing a combination of indexes from 1–12 and sequenced on an Illumina NextSeq on an Illumina NextSeq 2000 on a P2 flow cell (paired-end 75-bp reads).
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bioRxiv - Genomics 2024Quote: ... c) increasing PCR cycles (15 in total) and eliminating primer dimers prior to sequencing (Agencourt AMPure XP magnetic beads, Beckman Coulter). Primary PDAC were sequenced on the NovaSeq6000 ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... Samples were purified using 1.8X Agencourt AMPure XP PCR Purification beads according to the manufacturer’s instructions (Beckman Coulter, cat no A63881). The sheared DNA samples were quantified and qualified on a BioAnalyzer system using the DNA7500 assay kit (Agilent Technologies cat no ...
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bioRxiv - Genomics 2024Quote: ... the PCR product was cleaned up and followed by the 10X Genomics double size selection step using SPRIselect reagent (Beckman Coulter) to separate the protein library (2X SPRI ...
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bioRxiv - Genomics 2023Quote: PCR reaction products were cleaned and size selected using a 0.7:1 ratio of AMPure XP SPRI beads (Beckman Coulter Inc.) to sample ...
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bioRxiv - Genomics 2023Quote: ... The PCR amplification is followed by a bead clean up step with 1X sample-to-bead ratio AMPure XP beads (Beckman Coulter), binding at room temperature for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of samples treated with a single gRNA was subjected to purification with AMPure XP reagent (Beckman Coulter) according to the manufacturer’s manual using AMPure beads ...
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bioRxiv - Genomics 2023Quote: ... Full length transcripts were selected by PCR amplification using the LongAmp Taq Master Mix and the product was purified with AMPure XP beads (Beckman Coulter). An aliquot of 1 µL of Rapid Adapter was added to the amplified cDNA library ...
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bioRxiv - Molecular Biology 2023Quote: ... The content of the PCR tubes was pooled and mixed with an equal volume of AMPure XP beads (Beckman Coulter, A63881). After 5 min incubation ...
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bioRxiv - Immunology 2023Quote: ... The 50 μL PCR product was then purified by adding 40 μL of AMPure XP Beads (Beckman Coulter, cat. no: A63881) with some modifications to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The 50 μL PCR product was then purified by adding 40 μL of AMPure XP Beads (Beckman Coulter, cat. no: A63881) with some modifications to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: The 50 μL PCR product was then purified by adding 40 μL of AMPure XP Beads (Beckman Coulter, cat. no: A63881) with some modifications to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... except for the pre-PCR clean-up step where DNA was cleaned with 35 μL (0.76×) of AMPure XP beads (Beckman Coulter, A63881). 12 cycles of PCR were used to amplify the ChIP-seq library ...
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bioRxiv - Developmental Biology 2024Quote: ... Post-capture PCR enrichment was followed by final purification of the libraries with AMPure XP Beads (Beckman Coulter, catalog no. A63881). Library quality was evaluated using Bioanalyzer profiles (Agilent Technologies) ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were generated using a PCR-free approach (33) and then cleaned up using Agencourt AMPure XP SPRI beads (Beckman Coulter). The resulting libraries were sequenced on the Illumina NovaSeq platform at the Wellcome Sanger Institute ...
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bioRxiv - Immunology 2024Quote: ... The N26 barcode was PCR amplified using the above extracted plasmid as the template and final PCR products were purified by Ampure XP beads (Beckman Coulter), quantified ...
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bioRxiv - Genomics 2020Quote: ... After cleanup with the SPRIselect reagent kit (Beckman Coulter), the libraries were constructed by performing the following steps ...
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bioRxiv - Genomics 2020Quote: ... and the SPRIselect Reagent Kit (Beckman Coulter, Indianapolis, IN). Next ...
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bioRxiv - Genomics 2019Quote: ... and the SPRIselect Reagent kit (Beckman Coulter, Indianapolis, IN). Next ...
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bioRxiv - Microbiology 2021Quote: ... purified using the Agencourt AMPureXP kit (Beckman Coulter Genomics), and sequenced on an Illumina NextSeq 500 system (60 bp read length ...
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Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone receptor 1bioRxiv - Pharmacology and Toxicology 2022Quote: ... cAMP was measured using a RIA kit (Beckman Coulter), and inositol phosphates were separated by chromatographic separation of myo-[2-3H]inositol phosphates as described previously (29) ...
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bioRxiv - Microbiology 2022Quote: ... along with the Agencourt RNAdvance Tissue Kit (Beckman Coulter) and a KingFisher™ Flex Purification System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... After a cleanup with SPRIselect Reagent Kit (Beckman, C10640), final libraries were quantified using a Qubit™ 1X dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified using Agencourt RNAClean XP Kit (Beckman Coulter). The rRNA depleted cytoplasmic RNA and nuclear RNA were then treated with RNaseIII according to Ion Total RNA-Seq protocol v2 and purified with Magnetic Bead Cleanup Module (Thermo Fisher) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using the Agencourt DNAdvance Magnetic Bead Kit (Beckman Coulter) or the MagAttract HMW DNA Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... Amplified DNA was cleaned using a commercial kit (Beckman Coulter Agencourt AMPure XP ...
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bioRxiv - Synthetic Biology 2024Quote: ... kit and Agencourt AMPure XP beads (Beckman Coulter, USA), respectively ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... permeabilized using the IntraPrep Permeabilization Reagent Kit (Beckman Coulter) and stained with anti-Granzyme-APC ...
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bioRxiv - Physiology 2023Quote: ... Commercial radioimmunoassay kits (M/s Beckman Coulter IM 1188) with analytical sensitivity of 0.03 ng/mL and including a highly specific progesterone antibody were used for progesterone estimation ...
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bioRxiv - Neuroscience 2023Quote: ... Size selection with SPRIselect Reagent Kit (Beckman Coulter, B23318) was used to separate amplified cDNA molecules for 3’ gene expression and cell surface protein construction ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... permeabilized using the IntraPrep Permeabilization Reagent Kit (Beckman Coulter) and stained with anti-CD107a-PE or isotype control ...
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bioRxiv - Neuroscience 2020Quote: ... we generated double-stranded DNA template for transcription of each sgRNA via a template-free polymerase chain reaction (PCR) with partially overlapping primers (IDT, PAGE purified) and purified the product with Ampure XP beads (Beckman Coulter, A63880). We then transcribed sgRNAs in vitro using the MEGAscript T7 Transcription Kit (ThermoFisher Scientific ...
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bioRxiv - Genomics 2021Quote: Sequencing library amplified from the second round of PCR were size-selected and purified with a magnetic bead-based SPRIselect reagent (Beckman Coulter, B23318), and subjected to HiSeq4000 Illumina NGS platform using a single read (SR75 ...