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Citations for Diagenode :
1 - 50 of 320 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Real Time qPCR was performed by using a real-time PCR SYBR green master mix (Diagenode). Samples were run and analyzed on a Quantstudio 5 (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was quantified by real-time PCR using SYBR Green Master Mix (Diagenode) on a QuantStudio 6 instrument (Themo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was quantified by real-time PCR using SYBR Green Master Mix (Diagenode) on a QuantStudio 6 instrument (Themo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was quantified by real-time PCR using SYBR Green Master Mix (Diagenode) on a Quant Studio 5 384 well plate ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila in the samples was detected by diagnostic PCR using primers and probes of the R-DiaLegTM kit (Diagenode, Belgium). The PCRs were prepared in a final volume of 20 μL by adding the Master Mix 5X (TaqMan Probe LC2.0 ...
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bioRxiv - Microbiology 2023Quote: ... at room temperature (RT) for 30min and then quenched with glycine 0.125M (C01010062, Diagenode) at RT for 10min ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were sonicated three times in refrigerated Bioruptor Plus (Diagenode), 30 cycles 30sec ON/30sec OFF in a Diagenode TPX microtube M-50001 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were sonicated three times in refrigerated BIORUPTOR Plus (Diagenode), 10 cycles 30 sec ON - 30 sec OFF in a Diagenode TPX microtube M-50001 ...
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bioRxiv - Cell Biology 2021Quote: ... sonicated three times at maximum intensity (Bioruptor®, Diagenode, Belgium) and the samples were denaturated at 65 °C for 15 min ...
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bioRxiv - Genomics 2019Quote: ... cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 times for 30 s (maximum power, Bioruptor®plus, Diagenode). The lysate was clarified by ultracentrifugation (41 000 xG ...
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bioRxiv - Genomics 2023Quote: ... Samples were then vortexed (5 times) prior to sonication (Bioruptor, Diagenode), high setting ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were fixed for 10 minutes at RT in 11% formaldehyde solution in fixation buffer (Diagenode), diluted 1:10 in RPMI with 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... and sonicated 10 times 30s on/off in a Bioruptor sonicator (Diagenode). Lysates were diluted 10-fold and quantified by BCA assay against a BSA standard curve in diluted lysis buffer ...
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bioRxiv - Plant Biology 2023Quote: ... and sonicated four times (BioruptorTM, Diagenode, 30s on/1min off on High). They were quantified at 205 nm using the Nanodrop 2000 according to the manufacturer’s instruction (Thermoscientific ...
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bioRxiv - Genomics 2020Quote: ... total on-time 10 min) with a Biorupter® Pico Sonicator Device (Diagenode). 5 - 33 μg of sonicated chromatin was incubated with 4 μg ChIP-validated CTCF antibody (Active Motif ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were sonicated with a Bioruptor Pico sonicator (Diagenode, total time 8 min) and 4μg of antibodies were added to sheared chromatin (from 4 million cells for Olig2 and from 1 million cells for histone marks ...
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bioRxiv - Cancer Biology 2020Quote: ... After sonication (30”on/30” off, 10’ total time) in a Bioruptor (Diagenode) to disrupt aggregates ...
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bioRxiv - Immunology 2020Quote: ... The cells were cross-linked with 1% formaldehyde for 10 minutes at RT and quenched with 2.5 M glycine (Diagenode) for 5 minutes at RT ...
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bioRxiv - Cell Biology 2021Quote: ... we used a total processing time of 15min per sample in a Bioruptor (Diagenode) using the following settings ...
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bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 × 107 cells / immunoprecipitation were fixed for 30 minutes (min) at room temperature (RT) using Diagenode Crosslink Gold (Diagenode, Liège, Belgium). After two washing steps with PBS (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... Samples were sonicated two times for 10 minutes (30 seconds on, 30 seconds off) by Diagenode Bioruptor Pico ...
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bioRxiv - Genomics 2022Quote: ... Cell lysates were then sonicated 20 times at 4°C (Bioruptor Plus, Diagenode, 30/30 cycles) and centrifuged at 14000 rpm for 1 minute at 4°C to remove cellular debris ...
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bioRxiv - Molecular Biology 2021Quote: ... crushed with a Dounce homogenizer and sonicated ten times for 30 s (maximum power, Bioruptorplus, Diagenode). After centrifugation at 10,000g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13.000 rpm and 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13,000 rpm and 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and subjected to sonication (30 s on and 30 s off, 80 times, Bioruptor 300 [Diagenode]). The supernatant was collected by centrifugation ...
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bioRxiv - Genetics 2020Quote: ... 30 s ON/30 s OFF for 15 min × 3 times in Bioruptor Sonication System UCD200 (Diagenode) in 15 ml polystyrene conical tubes ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended nuclei were sonicated 5 times 30 sec on followed by 30 sec off using the Bioruptor (Diagenode). Insoluble proteins were removed by centrifugation for 10 min at 14,000 rpm at 4 °C.
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bioRxiv - Molecular Biology 2021Quote: ... crushed with a Dounce homogenizer and sonicated ten times for 30 s (maximum power, Bioruptor®plus, Diagenode). After centrifugation ...
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bioRxiv - Genomics 2019Quote: ... Resultant PCR products were sheared to ∼400bp using the Bioruptor Pico (Diagenode) and Nextera XT indexes added as previously described29 ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were sonicated 12 times (30 sec on, 30 sec off) at the high intensity in a Bioruptor (Diagenode) device ...
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bioRxiv - Plant Biology 2019Quote: ... sonicated in an ice bath four times for 30 seconds on high setting using a Bioruptor UCD-200 (Diagenode) with 1.5-minute breaks on ice and supplemented with Triton-X-100 to reach a final concentration of 2 % ...
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bioRxiv - Molecular Biology 2023Quote: ... After this time, samples were sonicated twice (7 cycles 30s on, 30s off) with a Bioruptor Plus sonicator (Diagenode). In between the two sonication cycles samples were incubated at 12°C with 0.8ul of Benzonase (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were sonicated for 5 cycles of 30 s on/off (2.5 min total sonication time) at 4°C in a Bioruptor Plus sonication system (Diagenode) and pelleted by centrifugation at 8000 RPM for 15 min at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... By ultrasonically treating the pellet three times for 30 s with intervals of 30 s with a Bioruptor (Diagenode), the proteins precipitated with acetone for 3 h at −30 °C were dispersed in 50 mM triethylammonium bicarbonate ...
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bioRxiv - Plant Biology 2020Quote: ... Nuclei were disrupted by sonication 10 times for 30 s each and 30 s of cooling in a Bioruptor (Diagenode). IPs were performed using α-GFP (Ab6556 ...
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bioRxiv - Genetics 2020Quote: ... The top 100 µl sperm suspension was sonicated at the medium level for five times with 3 seconds each (Bioruptor UCD-200; Diagenode). An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Cell Biology 2019Quote: ... 30” by 30” at mild intensity for myoblasts and five times for nascent myotubes using a Bioruptor UCD-200 (Diagenode). The samples were diluted to a final volume of 1 ml in ChIP buffer (16 mM Tris pH 8.1 ...
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bioRxiv - Cell Biology 2020Quote: ... the lysate was diluted 10 times with lysis buffer and subjected to sonication for 15 min in a refrigerated water bath (Bioruptor, Diagenode). Part of the sonicated chromatin was treated with proteinase K and RNase A and analyzed by electrophoresis in a 1.5% agarose gel to control the size of the DNA fragments (200-500 bp) ...
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bioRxiv - Plant Biology 2019Quote: ... The extracts were then distributed into 1.5 ml reaction tubes and sonicated in an ice bath four times for 30 seconds on high setting using a Bioruptor UCD-200 (Diagenode) with 1.5-minute breaks on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... 8 μg of gDNA were sonicated two times 7 min (30 sec ON /30 sec OFF) and two times 14 min (30 sec ON /30 sec OFF) with a bioruptor (Diagenode). Glucosylation and biotinylation of 5hmC were performed with the Hydroxymethyl Collector kit (Active Motif 55013) ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were sonicated five times (30-s pulses with 30-s break interval) using the Bioruptor water bath sonicator (Diagenode). The chromatin extracts were then centrifuged at 9,400 g for 10 min at 4 °C ...
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bioRxiv - Zoology 2023Quote: ... The lysate was pipetted up and down several times to homogenize the bee tissues and then sonicated (Bioruptor Pico,Diagenode) for 10 cycles consisting of a 30 s pulse and 30 s rest at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 % SDS in PBS) and sonicated 5 times for 5 min (30s ON/ 30s OFF) at high mode in a Bioruptor Pico (Diagenode). IP were performed overnight at 4°C using 1.5 mg of pre-coated epoxy Epoxy Dynabeads (M-270 ...
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bioRxiv - Molecular Biology 2023Quote: MeDIP-Seq libraries for Ion Torrent semiconductor sequencing were performed on the Ion Torrent PGM using a modified protocol from Ion Plus Fragment Library kit (Catalog # 4471252) combined with a 5-hydroxymethylcytosine (5hmC Kit, Catalog # AF-110–0016) immunoprecipitation kit (Diagenode) according to a previously optimized protocol (Guerrero-Bosagna & Jensen ...