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Citations for Diagenode :
1 - 50 of 310 citations for QuantiChrom Arginase Inhibitor Screening Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 500 μl of cold PBS + protease inhibitor (Diagenode kit). Nuclei preparation ...
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bioRxiv - Cell Biology 2023Quote: ... with protease inhibitors (Diagenode). Chromatin was then precleared with MagneGST magnetic beads (Promega ...
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bioRxiv - Developmental Biology 2022Quote: ... One hundred fifty microliters of Hank’s buffered salt solution with 1x protease inhibitor cocktail (in True MicroChIP kit, Diagenode) was added ...
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bioRxiv - Genetics 2019Quote: 0.5 × 106 frozen cells were resuspended in 250 µl buffer iL1 with proteinase inhibitors cocktail (iDeal ChIP-seq Kit for Histones, Diagenode) and incubated for 10 min at 4ºC on the Bohemian wheel ...
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bioRxiv - Molecular Biology 2022Quote: ... Protease inhibitor and RNase inhibitor) and sonicated for 10 cycles (30 secs on 30 secs off) on a Bioruptor (Diagenode) sonicator ...
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bioRxiv - Plant Biology 2020Quote: ... and protease inhibitor cocktail) and sonicated with a Bioruptor (Diagenode). The nuclei lysate was precipitated with anti-Flag (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... 1X Placental Rnase inhibitor) and sonicate using Bioruptor 300 (Diagenode) at high intensity for 60 cycles with 15 seconds ON and 45 seconds OFF each cycle ...
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bioRxiv - Cell Biology 2021Quote: ... 1X Placental Rnase inhibitor] and sonicate using Bioruptor 300 (Diagenode) at high intensity for 60 cycles with 15 seconds ON and 45 seconds OFF each cycle ...
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bioRxiv - Molecular Biology 2022Quote: ... protease inhibitor cocktail) and sonicated in the Bioruptor Pico (Diagenode) on low power at 15 seconds on 30 seconds off cycles until at least 70% cell membranes lysed and intact nuclei were visible ...
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bioRxiv - Immunology 2019Quote: ... 3 µL protease inhibitor cocktail 1µg of H3K27ac antibody (Diagenode) and incubated overnight at 4 ºC with rotation ...
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bioRxiv - Biochemistry 2021Quote: ... protease inhibitor cocktail) and sonicated in 4°C using Bioruptor (Diagenode) until lysate is clear ...
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bioRxiv - Cell Biology 2022Quote: ... protease inhibitor cocktail (Pierce #A32953) DNA was sheared using Biorup-tor (Diagenode), 20 cycles of 30 seconds on/30 seconds off ...
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bioRxiv - Neuroscience 2019Quote: ... 2.5X protease inhibitors EDTA-free) and sonicated with Bioruptor Power-up (Diagenode) to shear DNA into 150-700 bp fragments ...
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bioRxiv - Genetics 2023Quote: ... 0.1 % SDS) containing Complete proteinase inhibitor cocktail and sonicated with Picoruptor (Diagenode). After sonication debris was removed by centrifugation at 20,000 g for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: ... 10mM EDTA ph8 and proteinase inhibitors) in a Bioruptor Pico sonicating device (Diagenode). All ChIPs were incubated overnight with the respective antibodies (CTCF Active Motif 61311 ...
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bioRxiv - Genomics 2023Quote: ... the nuclei pellet was resuspended in 1mL of shearing buffer + protease inhibitor (Diagenode), incubated for 10 mins on ice ...
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bioRxiv - Genomics 2024Quote: ... proteinase inhibitor cocktail) and sonicated to fragments about 300 - 500 bp (Bioruptor, Diagenode). Dynabeads Protein A was washed twice with ChIP Buffer (10mM Tris-HCl pH7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... 150 mM NaCl and protease inhibitors) with a Bioruptor Sonication System (Diagenode, UCD-200). 20 μg of sonicated chromatin per sample was incubated overnight with 2-5 μg of antibody/protein A magnetic beads complexes ...
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The epigenomic landscape regulating organogenesis in human embryos linked to developmental disordersbioRxiv - Genomics 2019Quote: ... 1% SDS and protease inhibitor cocktail) and sonicated under prior optimised conditions (Diagenode Bioruptor). Sufficient sample was prepared to allow in parallel immunoprecipitation for H3K4me3 ...
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bioRxiv - Plant Biology 2023Quote: ... Protease Inhibitor Cocktail) and sonicated by using a Bioruptor® Plus sonication device (Diagenode) to obtain the desired DNA fragment size (enriched at 500 bp) ...
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bioRxiv - Cancer Biology 2020Quote: ... Fixed cells were washed and resuspended in sonication buffer supplemented with protease inhibitors (from Diagenode) and PhosStop (Roche) ...
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bioRxiv - Physiology 2021Quote: ... 10% glycerol) supplemented with protease and phosphatase inhibitor cocktails and sonicated in a Bioruptor (Diagenode). Protein lysates were quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were resuspended in RIPA Buffer containing complete protease inhibitors (EDTA-free) and sonicated (Bioruptor, Diagenode). Cellular debris was pelleted at 10 000 rpm for 10 min at 4°C and protein concentration determined ...
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bioRxiv - Neuroscience 2022Quote: ... 1x protease inhibitor cocktail (cOmplete™) was added and solution was sonicated using Bioruptor® (Diagenode). Following centrifugation the supernatant was collected and anti-FLAG antibody (F1804 ...
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bioRxiv - Molecular Biology 2023Quote: MeDIP-Seq libraries for Ion Torrent semiconductor sequencing were performed on the Ion Torrent PGM using a modified protocol from Ion Plus Fragment Library kit (Catalog # 4471252) combined with a 5-hydroxymethylcytosine (5hmC Kit, Catalog # AF-110–0016) immunoprecipitation kit (Diagenode) according to a previously optimized protocol (Guerrero-Bosagna & Jensen ...
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bioRxiv - Biochemistry 2024Quote: ... 1 x Halt Protease Inhibitor Cocktail) and water-bath sonicated (Diagenode; 25 cycles, 30 sec on and off). Sheard chromatin was diluted to achieve 0.1 % SDS and incubated with FOXA1 antibody (Novus Biologicals ...
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bioRxiv - Immunology 2022Quote: ... iPure kit v2 (Diagenode) was used ...
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bioRxiv - Genomics 2019Quote: ... MethylCap kit (Diagenode, C02020010) was employed to obtain the methylated DNA ...
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bioRxiv - Immunology 2021Quote: ... iPure kit v2 (Diagenode) was used ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was purified according to the IPure kit v2 Kit manual (Diagenode, C03010015). The amount of immunoprecipitated DNA was quantified by qPCR ...
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bioRxiv - Cell Biology 2021Quote: ... and 50 mM Tris-HCl (pH 8.1)] in the presence of protease inhibitors and subjected to sonication (Bioruptor, Diagenode) to generate chromatin fragments of ~300 bp in length ...
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bioRxiv - Genomics 2019Quote: ... Auto hMeDIP kit (Diagenode C02010033) was used according to manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... using LowCell# ChIP kit (Diagenode), with 1μg of antibody against ARNTL (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... or hMeDIP kit (Diagenode, C02010031). Quantification of precipitated DNA was determined using real-time PCR and the delta Ct method for relative quantitation (ABI 7900HT Fast Real-Time PCR System).
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bioRxiv - Cancer Biology 2021Quote: ... lysed in 1% SDS containing buffer supplemented with 1x protease and phosphatase inhibitors, and sonicated for 4 x 10 cycles (30 sec on, 30 sec off) in temperature-controlled Bioruptor 300 (Diagenode). Debris was removed by centrifuging at 14,000 rpm at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitor cocktails and sonicated for 1 min with the Bioruptor sonication device (Diagenode). Cell lysates were centrifuged at 13,000 × g for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... lysed with RIPA lysis buffer with protease inhibitors on ice for 15 minutes and then sonicated with a Bioruptor (Diagenode) for 10 minutes on high with 30 seconds on and 30 seconds off ...
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bioRxiv - Cancer Biology 2020Quote: ... 50mM Tris-Cl and protease inhibitor (Genetix)] followed by sonication for DNA fragmentation to an average fragment length of ∼500bp using Bioruptor (Diagenode). Sheared chromatin was incubated at 4°C overnight with 4μg of primary or isotype control antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... protease inhibitor cocktail), and sonicated 3x (15 sec on/30 sec off cycle, low power mode) in a Bioruptor (Diagenode). Nuclei were purified by sedimentation at 1,000 rpm for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 1X HALT protease inhibitor cocktail) and split into 250 μL volumes in 1.5 mL Bioruptor Plus TPX microtubes (Diagenode, #C30010010). Cells were sonicated at 4° C for 20 minutes using a waterbath sonicator cycling 30 seconds on and 30 seconds off (Bioruptor Pico ...
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bioRxiv - Cell Biology 2019Quote: ... in the presence of protease inhibitors and subjected to 3 × 10 cycles (30 seconds on and 30 seconds off) of sonication (Bioruptor, Diagenode) to generate chromatin fragments of ∼300 bp in length ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Nuclei were lysed in NLB (50 mM Tris-HCl pH8, 10 mM EDTA, 1% SDS and 1x Proteases Inhibitor cocktail. Chromatin was sonicated using a Diagenode Bioruptor (40 Hz ...
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bioRxiv - Immunology 2022Quote: ... with a freshly added protease inhibitor cocktail and sodium vanadate and subjected to 9 cycles (30s ON-10s OFF) of ultrasonication (Bioruptor Plus; Diagenode). The sonicated samples are then spun down ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1X protease inhibitor (Genetix) followed by DNA fragmentation through sonication to an average fragment length of ∼500 bp using Bioruptor (Diagenode) and sheared chromatin was incubated at 4°C overnight with 4µg of primary β-catenin (CST ...
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bioRxiv - Plant Biology 2023Quote: ... pH 8.0 and protease inhibitor cocktail) and sheared into 200-500 bp fragments with a sonicator (Diagenode, Bioruptor Plus, Belgium). The sonicated chromatin complex was immunoprecipitated by anti-H2A.Z and isolated by binding to Dynabeads Protein A/G (26162 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was resuspended in ice-cold lysis buffer (1xPBS, 2 mM PMSF, 2 mM DTT, 1 x protease inhibitors (Pierce)) and sonicated (Bioruptor Pico, Diagenode) at 4LJ with alternating 10 s burst/10 s break ...
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bioRxiv - Cancer Biology 2023Quote: ... the fixed cells were lysed in SDS based lysis buffer with 2X mammalian Protease Inhibitor Cocktail and subjected to chromatin fragmentation to an average size range of 200-300bp using Bioruptor (Diagenode). 10% fragmented chromatin was processed as Input fraction via Phenol-Chloroform-Isoamyl Alcohol (PCI ...
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bioRxiv - Genomics 2023Quote: ... 1x protease inhibitor) and sonicated for 10 cycles (sonication cycle: 30 sec ON, 30 sec OFF) using Bioruptor Plus (Diagenode). The samples were centrifugated at 14000x g for 10 minutes at 4°C and the supernatant containing the soluble chromatin was transferred to a fresh low-binding tube ...
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bioRxiv - Cancer Biology 2024Quote: ... cells pellets were then resuspended in modified RIPA buffer (50nm Tris pH 7.5, 150nM NaCl, 5mM EDTA, 50nM NaF, both inhibitors) and sonicated using Bioruptor® Pico (Diagenode). To remove the possibility of any DNA-dependent protein interactions ...