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Citations for Diagenode :
1 - 50 of 176 citations for Erythromycin 90 95% Erythromycin A N N Dimethyl 13C2 ~90% 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Genomics 2022Quote: ... 90 μg of DNA was purified then sheared to 20 kb fragments using the megaruptor1 system (Diagenode). For each library ...
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bioRxiv - Genetics 2020Quote: ... The DNA was sonicated for 2 cycles of 15sec ON/90 sec OFF in a bioruptor (Diagenode) at 4°C ...
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bioRxiv - Genomics 2023Quote: ... 0.5% N-lauroylsarcosine) and sonicated using Bioruptor Pico (Diagenode) in 15mL tubes for 20 cycles of 30 s ON and 30 s OFF pulses in 4°C bath ...
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bioRxiv - Systems Biology 2019Quote: ... they were lysed after addition of MTBE by sonication (Bioruptor, Diagenode, Belgium). The dried lipid extracts from all samples were reconstituted in BuOH:IPA:water in a ratio 8:23:69 (v/v/v ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated O/N with 0.25µg of H3K9me3 (C15410193-Diagenode).
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
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bioRxiv - Genomics 2023Quote: Two micrograms of genomic DNA were sonicated for 6 cycles (6 s on, 90 s off) at 4 °C with a Bioruptor NGS (Diagenode), generating average fragments of 1 kb ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We reserved 10% of the pooled fragmented sample for the input (fragment control) and used the remaining 90% to perform the chromatin pull‐down (ChIP sample, target antibody: H3K9me3 polyclonal classic Diagenode C15410056) according to the 100,000 cell specifications ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We reserved 10% of the pooled fragmented sample for the input (fragment control) and used the remaining 90% to perform the chromatin pull-down (ChIP sample, target antibody: H3K9me3 polyclonal classic Diagenode C15410056) according to the 100,000 cell specifications ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.5% N-lauroylsarcosine) and sonicated in a water bath sonicator (Diagenode Bioruptor ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5% N-lauroylsarcosine) and sonicated for 30 minutes using a BioRuptor Pico (Diagenode), shearing genomic DNA to an average size of 0.5 kb ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 0.5% SDS and 0.2% n-lauroylsarcosine and sonicated using a Bioruptor (Diagenode) with high power settings to obtain an average fragment size distribution of 200-500 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA concentration was normalized (except for low quality samples) and mechanically fragmented to 300-600 bp fragments using a Bioruptor Pico (Diagenode; 15s ON, 90s OFF, eight cycles). Low quality DNA were then reconcentred with AMPure XP beads and libraries were constructed according to the NEBNext® Ultra II DNA Library Prep for Illumina® kit recommendations (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% N-lauroylsarcosine) and sonicated for 25 cycles (30 sec on/off) using a BioRuptor Pico sonicator (Diagenode), shearing genomic DNA to produce fragments between 300bp and 1kb ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The H3K9me3 antibody (Diagenode, 1.65 ug/ul) was incubated for >3 hours with Dynabeads Protein G to bind the antibody to the beads ...
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bioRxiv - Cancer Biology 2021Quote: ... sodium deoxycholate and 0.5% (vol/vol) N-lauroylsarcosine) and the chromatin was sheared to 300 – 800 base pair (bp) fragments by Bioruptor (Diagenode, Belgium) 12 cycles of 30 second “ON” and 30 second “OFF” intervals ...
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bioRxiv - Developmental Biology 2020Quote: ... and antibody (1.2 ug of anti-H3K4me1: Diagenode, C15410194 ...
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bioRxiv - Immunology 2023Quote: ... 0.1% deoxycholate and 0.5% N-lauroylsarcosine) and split into two in 15ml polypropylene tubes suitable for the Bioruptor® Pico (Diagenode, Catalog # C01020031). One lid of a 1.5ml tube was filled with sonication beads and added to each sample ...
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bioRxiv - Neuroscience 2020Quote: PFF transduction was conducted at 10 DIV by adding 2 ug/ml (96 well plate and coverslips) or 10 ug/ml (biochemistry) of PFFs diluted in PBS and sonicated with a BioRuptor (Diagenode) bath sonicator for 10 cycles of 30 seconds on ...
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bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... boiled for 5 min at 95°C 1000rpm in 2% SDC buffer (2% SDC, 100 mM Tris-HCl pH=8.5) and sonicated (Diagenode Bioruptor, 15 * 30s at high intensity). Protein concentration was determined via BCA Protein Assay (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS and 0.5% N-lauroylsarcosine) and the chromatin was fragmented by sonication for 10 cycles on Bioruptor (Diagenode high settings, 30’’ on/off). Chromatin was separated from cell debris using centrifugation and stored at -80C for maximum of 2 months.
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bioRxiv - Cancer Biology 2020Quote: ... Chromatin was immunoprecipitated with 10 ug of H3K27Ac antibody (Diagenode cat# C15410196), 10 ug of ASCL1 antibody (abcam ab74065 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 ug/ul of lysates were sonicated with a Bioruptor Plus (Diagenode) for 20 cycles (30 seconds ON and 30 seconds OFF) ...
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bioRxiv - Synthetic Biology 2023Quote: ... heated at 95°C for 10 min and sonicated with the Bioruptor sonicator (Diagenode) for 15 cycles (30 s ON ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were denatured at 95°C and DNA was sonicated using a Bioruptor 300 (Diagenode) on the high setting for 8 cycles 30 sec ON / 30 sec OFF ...
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bioRxiv - Developmental Biology 2019Quote: ... 2.5 μ 10% Triton X-100 and 1 l of anti-methylcytidine antibody (1 mg/ml Diagenode # MAb-081-μ 100) overnight ...
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bioRxiv - Genomics 2022Quote: ... 5 ug of genomic DNA was sheared to approximately 15-20 kb using Megaruptor3 (Diagenode). The sheared DNA was treated with DNA Prep to remove single-stranded ends ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... and subsequently lysed by 5min boiling at 95°C and sonication (Bioruptor, model UCD-200, Diagenode) for 15min ...
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bioRxiv - Microbiology 2023Quote: ... 1 ug of genomic DNA was sheared by sonication using a Picorupter (Diagenode s.a., Liege, Belgium) to obtain fragments with an average size of 300 bp ...
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bioRxiv - Cancer Biology 2019Quote: ... and subsequently lysed by boiling for 5 min at 95°Cand sonication (Bioruptor, model ACD-200, Diagenode) for 15 min at level 5 (30 sec ON ...
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bioRxiv - Genetics 2023Quote: ... The mixture was heated to 95°C for 10 min and sonicated in a Bioruptor (Diagenode, Liege, Belgium) (30 sec sonication ...
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bioRxiv - Genomics 2023Quote: 10 ug of C5 genomic DNA was sheared to 30 kb - 50 kb using the Megaruptor 3 (Diagenode). The sheared DNA was treated with exonuclease to remove single-stranded ends ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... SDS lysates were boiled at 95°C for 10 minutes and then 10 cycles in a Bioruptor Pico (Diagenode) of 30 seconds on and 30 second off per cycle ...
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Deep learning the collisional cross sections of the peptide universe from a million training samplesbioRxiv - Systems Biology 2020Quote: ... The samples were boiled at 95°C for 10 min and subsequently sonicated at maximum power (Bioruptor, Diagenode, Belgium). Proteolytic digestion was performed overnight at 37 °C by adding either i ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended samples were boiled for 5 min at 95°C and sonicated for 15 min in the Bioruptor (Diagenode) at 30 sec on ...
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bioRxiv - Microbiology 2023Quote: ... 40 mM 2-Chloroacetamide, 50 mM HEPES pH 8.5), boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). For the intracellular proteomes ...
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bioRxiv - Microbiology 2023Quote: ... was dissolved in 75 µL lysis buffer, boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). Lysates from the secretomes and intracellular fractions were centrifuged (10 min ...
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bioRxiv - Bioengineering 2024Quote: ... The cell lysates were heated at 95[ for 5 minutes and sonicated for 15 min using a Bioruptor Plus (Diagenode). The supernatant was collected after centrifugation at 18,000 g for 30 min at 4[ ...
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bioRxiv - Biochemistry 2022Quote: ... heated to 95°C for 10 min shaking (1000 rpm) followed by 2 cycles à 20 s of sonication in BioRuptor (Diagenode). Aliquots containing 50 μg protein were transferred into a cartridge and cooled ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein was extracted by incubating the islets with Laemmli buffer at 95°C for 5 mins and homogenized for another 5 mins in a sonication device (Diagenode, B01060001) at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... 100 mM Tris-HCl pH 8.5) and boiled for 7 min at 95 °C before high-energy sonication to shear DNA (Diagenode Bioruptor pico). Protein concentrations were determined via a BCA assay (Thermo Fisher) ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2 ug of genomic DNA was fragmented into 200-300 bp using the Bioruptor UCD-200 sonicator (Diagenode, Denville, NJ). Libraries were constructed using the DNA library prep kit for BGI platform (BGI ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 95°C for 10 min and sonicated for 5 min using a Bioruptor® Plus sonication device (Diagenode, Liège, Belgium). Protein lysates were then run on a precast NuPAGE Bis-Tris 4–12% SDS–PAGE (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and the samples were heated for 5 min at 95°C prior to sonication with a Bioruptor UCD-200 (Diagenode, Seraing, Belgium) device for ten cycles of 1 min pulse and 30 sec pause at high power ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % Triton X-100) supplemented with 1 x HALT in a 15 ml Bioruptor tube containing one scoop of sonication beads (Diagenode) and sonicated in Bioruptor Pico (Diagenode ...
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bioRxiv - Cancer Biology 2022Quote: ... 200 mM NaCl) supplemented with 100 μg/ml ssDNA and PI and sonicated using Bioruptor Pico sonicator (Diagenode, Denville, NJ, USA). In order to reach DNA fragments in the range of 200-700 bp ...
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bioRxiv - Neuroscience 2022Quote: ... PFFs were diluted in 1× PBS to a final concentration of 100 μL/Ml and sonicated with Bioruptor sonication device (Diagenode, Liege, Belgium, #B01020001) at the following settings ...