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Citations for Diagenode :
1 - 50 of 116 citations for 15 PGDH HPGD Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysis was carried out by sonication for 15 min (15s ON/15s OFF, medium intensity, Bioruptor, Diagenode) at 4°C in 100 µL/5000 tardigrades pellet of the following solution 12mM sodium deoxycholate ...
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bioRxiv - Molecular Biology 2023Quote: ... in 15 ml sonication tubes (Diagenode, C01020031) using 350 mg/mL of sonication beads (Diagenode ...
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bioRxiv - Plant Biology 2020Quote: ... samples were placed on ice for 10 min and sonicated for 6 cycles 15 sec “on” – 15 sec “off” using Bioruptor Plus (Diagenode). After that ...
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bioRxiv - Developmental Biology 2019Quote: ... and sonication for 2 cycles of 15s ON and 15s OFF on medium power at 4°C on a PicoBioruptor (Diagenode). After 10 minutes incubation at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... and sonicated for 2 cycles of 15s ON and 15s OFF on full power at 4°C on a Bioruptor (Diagenode). Samples were incubated for 1 hour on a rotating wheel at 4°C and centrifuged 10 minutes at 12,000 g and 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were extracted by sonication executing 40 cycles of 15s on and 15 s off at 4 °C using a Bioruptor plus (model UCD-300, Diagenode). After lysis ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell suspensions in 0.6 mL µtube were sonicated in water bath at 4°C during 12 min (15 sec. ON, 15 sec. OFF) with a Bioruptor apparatus (Diagenode) setted on high power and then clarified by 10 min centrifigation at 10 000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... sonicated for 15 min with a Bioruptor (Diagenode) and centrifuged for 5 min at 16,000 × g at room temperature ...
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bioRxiv - Genomics 2019Quote: ... transferred to 15-ml Sumilon TPX tubes (Diagenode C30010009) and sonicated using a Diagenode Bioruptor 300 at 4°C and high power ...
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bioRxiv - Neuroscience 2020Quote: ... then sonicated for 15 minutes using Biorupter sonicator (Diagenode). On the day of the assay ...
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bioRxiv - Developmental Biology 2020Quote: ... Worms were distributed into 15 ml TPX tubes (Diagenode) to reach 200–400 µl worm pellet per tube ...
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bioRxiv - Molecular Biology 2021Quote: Samples were sonicated for 15 cycles using a Bioruptor (Diagenode), to generate ∼ 500bp average fragment size ...
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bioRxiv - Genetics 2023Quote: ... and control anti IgG antibody (Diagenode, Cat. No. #C15400001-15). Pulled-down DNA fragments were analyzed via qPCR using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Cell extracts were sonicated for 15 min with a Bioruptor (Diagenode) to obtain 500-bp DNA fragments ...
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bioRxiv - Synthetic Biology 2021Quote: ... The nuclei were sonicated utilizing 15 ml Bioruptor tubes (Diagenode, C01020031) with 437.5 mg sonication beads (Diagenode ...
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bioRxiv - Genomics 2023Quote: ... Chromatin was fragmented in 15 mL tubes using the bioruptor (Diagenode) for 12 cycles (30 sec on/30 sec off) ...
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bioRxiv - Molecular Biology 2021Quote: ... and sonicated using a Bioruptor (Diagenode, medium power, 3 x 15 sec). Protein concentration was measured using the Bradford assay (Bio-Rad) ...
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bioRxiv - Cancer Biology 2019Quote: ... Chromatin was sonicated for 15 min in a Bioruptor water bath (Diagenode) using high intensity and 30”on/30”off cycles and then diluted to adjust SDS concentration to 0.1% ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin was sheared by sonication in 15 ml Bioruptor tubes (Diagenode, C01020031) with 437.5 mg sonication beads (Diagenode ...
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bioRxiv - Genetics 2021Quote: Chromatin was transferred to a 15 ml conical tube (Diagenode, cat. # C30010017) for sonication in a Bioruptor Pico instrument (Diagenode ...
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bioRxiv - Physiology 2023Quote: ... Lysates were sonicated for 15 min (Bioruptor, Diagenode, cycles of 30 sec) and protein concentrations were determined using the Pierce BCA Protein Assay (Thermo) ...
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bioRxiv - Biophysics 2024Quote: ... Chromatin was sheared by sonication in 15 ml Bioruptor tubes (Diagenode, C01020031) with 437.5 mg sonication beads (Diagenode ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were further sonicated (15 cycles at high intensity using a Bioruptor, Diagenode) and boiled (90°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 15-20 kb DNA fragments were obtained with Megaruptor (Diagenode, Denville, NJ, USA) and checked on a Fragment Analyzer (Advanced Analytical Technologies ...
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bioRxiv - Genetics 2024Quote: ... Lysates were sonicated for 4×15 minutes using a Bioruptor UCD-200 (Diagenode). Hybridization of probes was performed for 16 hours ...
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bioRxiv - Microbiology 2021Quote: ... The genomic DNA was sheared to ∼15-20 kb size by Megaruptor (Diagenode B06010001). The sheared DNA samples were then concentrated and purified by AMPure PB Beads (Pacific Biosciences 100-265-900 ...
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bioRxiv - Cancer Biology 2021Quote: ... and sonicated using a bioruptor (Diagenode, 15 min 30 sec ON /30 sec OFF). Sonicated chromatin was incubated at 4°C overnight with either an Anti-H3K4me3 (Millipore 04-745 ...
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bioRxiv - Biochemistry 2020Quote: ... samples were sonicated for 15 minutes (37°C) using a Bioruptor plus ultrasonicator (Diagenode). The second digestion step was done using fresh LysC (1:50 enzyme to protein ratio ...
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bioRxiv - Immunology 2020Quote: ... Samples were sonicated for 15 cycles (30s on/30s off) on a bioruptor (Diagenode). Protein concentration was determined using the BCA assay and about 30 μg of proteins were digested ...
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bioRxiv - Neuroscience 2021Quote: ... 1mg/mL BSA) and sonicating at full strength for 15 min (Diagenode, Bioruptor Plus). Protein concentration was measured using a Bradford Assay and normalized so that all assay inputs contained the same amount of protein ...
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bioRxiv - Microbiology 2023Quote: ... Samples were sonicated using a Bioruptor Plus from Diagenode (15 cycles of 30 s). The protein concentration was measured using a tryptophan assay ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were transferred into 15-ml TPX tubes and sonicated using Bioruptor Plus (Diagenode) with the following parameters ...
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bioRxiv - Biochemistry 2020Quote: ... and transferred (about 1.6 ml/tube) into 15 ml-Bioruptor® Plus TPX tubes (Diagenode). Sonication was performed with a Bioruptor® Plus sonication device (Diagenode) ...
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bioRxiv - Molecular Biology 2020Quote: ... This was followed by sonication of DNA for 15 min using the BioRuptor Pico (Diagenode), shearing genomic DNA to an average size of less than 1 kb ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclei were incubated for 15 min on ice followed by sonication with a BioRuptor (Diagenode) (18 cycles ...
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bioRxiv - Genomics 2022Quote: ... 5 ug of genomic DNA was sheared to approximately 15-20 kb using Megaruptor3 (Diagenode). The sheared DNA was treated with DNA Prep to remove single-stranded ends ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated for 15 min at high power with the Bioruptor sonicator (Diagenode). Protein quantification was performed using the Bradford reagent (Biorad #5000001) ...
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bioRxiv - Genetics 2024Quote: ... Cell lysates were transferred to Diagenode 15 ml sonication tubes and sonicated (Bioruptor pico, Diagenode) 30 sec ON/ 45 sec OFF at 4°C for 60 cycles (6x10) ...
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bioRxiv - Genetics 2019Quote: ... we used ChIP grade antibodies specific to human H3K27ac histones (Diagenode). A negative control undergoing no IP (input ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoprecipitation was done using 15-20 μg of DNA and 1 μg of CTCF Ab (Diagenode:C15410210) and all steps were performed following the manufacturer instructions ...
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bioRxiv - Genomics 2021Quote: ... After sonication (15 sec on/ 30 sec off, 5 cycles, 4 C) using Bioruptor 300 (Diagenode) 5 µl were stained with DAPI to verify isolation of intact Macs ...
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin was sheared in Bioruptor® Pico 15 mL tubes with 300 µL beads (Diagenode C30010017) by 25 cycles of 30 sec on and 30 sec off using a Bioruptor® (Diagenode B01060010) ...
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bioRxiv - Cell Biology 2023Quote: ... for 15 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016). Finally ...
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bioRxiv - Microbiology 2023Quote: ... Purification steps were performed using AMPure PB beads (PacBio).15 μg of DNA was purified then sheared at 20 kb using the Megaruptor1 system (Diagenode). Using SMRTbell Express Template prep kit 2.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... for 15 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... cells lysates were sonicated (15 cycles: 10 sec. on/10 sec. off) using sonicator (Bioruptor, Diagenode) and centrifuged at 12,000 g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 sec off) for 15 minutes to shear chromatin to less than 500 bp with Bioruptor (Diagenode). After pelleting the insoluble fraction ...
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bioRxiv - Developmental Biology 2019Quote: ... DNA was crosslinked in formaldehyde and sonicated in a Bioruptor for 15 minutes at high power (Diagenode). Cells were resuspended in a total volume of 250 ml of Buffer A and Buffer B mix (half of the recommended volume for 0.5×106cells) ...
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bioRxiv - Plant Biology 2019Quote: ... The extracts were sonicated in 15 ml tubes in an ice bath using Bioruptor UCD-200 (Diagenode) on high setting ...